Title: Proteomic analysis of mouse liver plasma membrane: Use of differential extraction to enrich hydrophobic membrane proteins
Abstract: PROTEOMICSVolume 5, Issue 17 p. 4510-4524 Regular Article Proteomic analysis of mouse liver plasma membrane: Use of differential extraction to enrich hydrophobic membrane proteins Lijun Zhang, Lijun Zhang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorJinyun Xie, Jinyun Xie College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXi'e Wang, Xi'e Wang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXiaohui Liu, Xiaohui Liu College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXinke Tang, Xinke Tang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorRui Cao, Rui Cao College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorWeijun Hu, Weijun Hu College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorSong Nie, Song Nie College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorChunming Fan, Chunming Fan College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorSongping Liang, Corresponding Author Songping Liang [email protected] College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaCollege of Life Science, Hunan Normal University, Changsha, 410081, P. R. China Fax: +86-731-886-1304===Search for more papers by this author Lijun Zhang, Lijun Zhang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorJinyun Xie, Jinyun Xie College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXi'e Wang, Xi'e Wang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXiaohui Liu, Xiaohui Liu College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorXinke Tang, Xinke Tang College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorRui Cao, Rui Cao College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorWeijun Hu, Weijun Hu College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorSong Nie, Song Nie College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorChunming Fan, Chunming Fan College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaSearch for more papers by this authorSongping Liang, Corresponding Author Songping Liang [email protected] College of Life Science, Hunan Normal University, Changsha 410081, P. R. ChinaCollege of Life Science, Hunan Normal University, Changsha, 410081, P. R. China Fax: +86-731-886-1304===Search for more papers by this author First published: 17 November 2005 https://doi.org/10.1002/pmic.200401318Citations: 56AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract To comprehensively identify proteins of liver plasma membrane (PM), we isolated PMs from mouse liver by sucrose density gradient centrifugation. An optimized extraction method for whole PM proteins and several methods of differential extraction expected to enrich hydrophobic membrane proteins were tested. The extracted PM proteins were separated by 2-DE, and were identified by MALDI-TOF-MS, and ESI-quadrupole-TOF MS. As the complementary method, 1-DE-MS/MS was also used to identify PM proteins. The optimized lysis buffer containing urea, thiourea, CHAPS and NP-40 was able to extract more PM proteins, and treatment of PM samples with chloroform/methanol and sodium carbonate led to enrichment of more hydrophobic PM proteins. From the mouse liver PM fraction, 175 non-redundant gene products were identified, of which 88 (about 50%) were integral membrane proteins with one to seven transmembrane domains. The remaining products were probably membrane-associated and cytosolic proteins. The function distribution of all the identified liver PM proteins was analyzed; 40% represented enzymes, 12% receptors and 9% proteins with unknown function. Citing Literature Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2120/2005/pro1318_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume5, Issue17No. 17 November 2005Pages 4510-4524 RelatedInformation
Publication Year: 2005
Publication Date: 2005-10-13
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 76
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot