Title: Synergistic Effects of Plant Extracts and Antibiotics on Staphylococcus aureus Strains Isolated from Clinical Specimens
Abstract: This study has been done to evaluate the interaction between water extracts of Psidium guajava, Rosmarinus officinalis, Salvia fruticosa, Majorana syriaca, Ocimum basilucum, Syzygium aromaticum, Laurus nobilis and Rosa damascena alone and then synergy testing of these extracts with known antimicrobial agents of different mechanisms (protein synthesis inhibition: oxytetracycline HCl and gentamicin sulfate; cell wall synthesis inhibition: penicillin G and cephalexin; folic acid synthesis inhibition: Sulfadimethoxine as sodium; and nucleic acid synthesis inhibition: enrofloxacin) using both well-diffusion and microdilution method. This study was conducted against five S. aureus isolates; one is Methicillin-resistant Staphylococcus aureus (MRSA) and 4 Methicillin-sensitive Staphylococcus aureus (MSSA). The results of the conducted experiments using well-diffusion method demonstrate that these plants showed in vitro interactions between antimicrobial agents and plant extracts were additive against the five strains of S. aureus, while using microdilution method showed synergistic effects between combination of antibiotics and plant extracts with significant reduction in the MICs of the test antibiotics against these strains of S. aureus. This change in MIC was noticed in all plant extracts against test antibiotics including these plants showed weak antibacterial activity by well diffusion method. Also our results showed that synergism effect between antimicrobial agent and plant extract was occurred in both sensitive and resistant strains but the magnitude of minimum fold inhibition in resistant strains especially MRSA strain was higher than the sensitive strains.
Publication Year: 2009
Publication Date: 2009-06-01
Language: en
Type: article
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Cited By Count: 21
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