Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2103687648', 'doi': 'https://doi.org/10.1093/protein/gzn002', 'title': 'Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties', 'display_name': 'Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties', 'publication_year': 2008, 'publication_date': '2008-01-31', 'ids': {'openalex': 'https://openalex.org/W2103687648', 'doi': 'https://doi.org/10.1093/protein/gzn002', 'mag': '2103687648', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/18239072'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1093/protein/gzn002', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S44492648', 'display_name': 'Protein Engineering Design and Selection', 'issn_l': '1741-0126', 'issn': ['1741-0126', '1741-0134'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5074421080', 'display_name': 'Markus Alahuhta', 'orcid': 'https://orcid.org/0000-0001-9375-778X'}, 'institutions': [{'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}], 'countries': ['FI'], 'is_corresponding': False, 'raw_author_name': 'M. Alahuhta', 'raw_affiliation_strings': ['Biocenter Oulu', 'Department of Biochemistry'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry', 'institution_ids': []}, {'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5022018882', 'display_name': 'Mikko Salin', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}], 'countries': ['FI'], 'is_corresponding': False, 'raw_author_name': 'M. Salin', 'raw_affiliation_strings': ['Biocenter Oulu', 'Department of Biochemistry'], 'affiliations': [{'raw_affiliation_string': 'Department of Biochemistry', 'institution_ids': []}, {'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5000387665', 'display_name': 'Marco G. Casteleijn', 'orcid': 'https://orcid.org/0000-0001-6617-1513'}, 'institutions': [{'id': 'https://openalex.org/I98381234', 'display_name': 'University of Oulu', 'ror': 'https://ror.org/03yj89h83', 'country_code': 'FI', 'type': 'education', 'lineage': ['https://openalex.org/I98381234']}, {'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}], 'countries': ['FI'], 'is_corresponding': False, 'raw_author_name': 'M. G. Casteleijn', 'raw_affiliation_strings': ['Biocenter Oulu', 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland'], 'affiliations': [{'raw_affiliation_string': 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland', 'institution_ids': ['https://openalex.org/I98381234']}, {'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5053381061', 'display_name': 'Christian Kemmer', 'orcid': 'https://orcid.org/0000-0002-1012-5128'}, 'institutions': [{'id': 'https://openalex.org/I98381234', 'display_name': 'University of Oulu', 'ror': 'https://ror.org/03yj89h83', 'country_code': 'FI', 'type': 'education', 'lineage': ['https://openalex.org/I98381234']}, {'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}], 'countries': ['FI'], 'is_corresponding': False, 'raw_author_name': 'C. Kemmer', 'raw_affiliation_strings': ['Biocenter Oulu', 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland'], 'affiliations': [{'raw_affiliation_string': 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland', 'institution_ids': ['https://openalex.org/I98381234']}, {'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5088451915', 'display_name': 'Ibrahim El Tantawy El Sayed', 'orcid': 'https://orcid.org/0000-0003-1227-7931'}, 'institutions': [{'id': 'https://openalex.org/I149213910', 'display_name': 'University of Antwerp', 'ror': 'https://ror.org/008x57b05', 'country_code': 'BE', 'type': 'education', 'lineage': ['https://openalex.org/I149213910']}], 'countries': ['BE'], 'is_corresponding': False, 'raw_author_name': 'I. El-Sayed', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium', 'institution_ids': ['https://openalex.org/I149213910']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5013840465', 'display_name': 'Koen Augustyns', 'orcid': 'https://orcid.org/0000-0002-5203-4339'}, 'institutions': [{'id': 'https://openalex.org/I149213910', 'display_name': 'University of Antwerp', 'ror': 'https://ror.org/008x57b05', 'country_code': 'BE', 'type': 'education', 'lineage': ['https://openalex.org/I149213910']}], 'countries': ['BE'], 'is_corresponding': False, 'raw_author_name': 'K. Augustyns', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium', 'institution_ids': ['https://openalex.org/I149213910']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5001739345', 'display_name': 'Peter Neubauer', 'orcid': 'https://orcid.org/0000-0002-1214-9713'}, 'institutions': [{'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}, {'id': 'https://openalex.org/I98381234', 'display_name': 'University of Oulu', 'ror': 'https://ror.org/03yj89h83', 'country_code': 'FI', 'type': 'education', 'lineage': ['https://openalex.org/I98381234']}], 'countries': ['FI'], 'is_corresponding': False, 'raw_author_name': 'P. Neubauer', 'raw_affiliation_strings': ['Biocenter Oulu', 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland'], 'affiliations': [{'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}, {'raw_affiliation_string': 'Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland', 'institution_ids': ['https://openalex.org/I98381234']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5111521914', 'display_name': 'R.K. Wierenga', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210122519', 'display_name': 'Biocenter Finland', 'ror': 'https://ror.org/03aay6c48', 'country_code': 'FI', 'type': 'facility', 'lineage': ['https://openalex.org/I4210122519']}], 'countries': ['FI'], 'is_corresponding': True, 'raw_author_name': 'R. K. Wierenga', 'raw_affiliation_strings': ['Biocenter Oulu', 'Department of Biochemistry'], 'affiliations': [{'raw_affiliation_string': 'Biocenter Oulu', 'institution_ids': ['https://openalex.org/I4210122519']}, {'raw_affiliation_string': 'Department of Biochemistry', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 3, 'corresponding_author_ids': ['https://openalex.org/A5111521914'], 'corresponding_institution_ids': ['https://openalex.org/I4210122519'], 'apc_list': None, 'apc_paid': None, 'fwci': 1.929, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 16, 'citation_normalized_percentile': {'value': 0.826332, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 86, 'max': 87}, 'biblio': {'volume': '21', 'issue': '4', 'first_page': '257', 'last_page': '266'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10839', 'display_name': 'Pancreatic function and diabetes', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2746', 'display_name': 'Surgery'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10839', 'display_name': 'Pancreatic function and diabetes', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2746', 'display_name': 'Surgery'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12286', 'display_name': 'Erythrocyte Function and Pathophysiology', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/2737', 'display_name': 'Physiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12827', 'display_name': 'Biochemical and Molecular Research', 'score': 0.9875, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/triosephosphate-isomerase', 'display_name': 'Triosephosphate isomerase', 'score': 0.5419577}, {'id': 'https://openalex.org/keywords/a-site', 'display_name': 'A-site', 'score': 0.41182357}], 'concepts': [{'id': 'https://openalex.org/C41183919', 'wikidata': 'https://www.wikidata.org/wiki/Q423026', 'display_name': 'Active site', 'level': 3, 'score': 0.8882884}, {'id': 'https://openalex.org/C107824862', 'wikidata': 'https://www.wikidata.org/wiki/Q616005', 'display_name': 'Binding site', 'level': 2, 'score': 0.70583826}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.57475656}, {'id': 'https://openalex.org/C2778727959', 'wikidata': 'https://www.wikidata.org/wiki/Q24777683', 'display_name': 'Triosephosphate isomerase', 'level': 3, 'score': 0.5419577}, {'id': 'https://openalex.org/C71240020', 'wikidata': 'https://www.wikidata.org/wiki/Q186011', 'display_name': 'Stereochemistry', 'level': 1, 'score': 0.5324653}, {'id': 'https://openalex.org/C112887158', 'wikidata': 'https://www.wikidata.org/wiki/Q169324', 'display_name': 'Hydrogen bond', 'level': 3, 'score': 0.51188767}, {'id': 'https://openalex.org/C176944494', 'wikidata': 'https://www.wikidata.org/wiki/Q1415380', 'display_name': 'Point mutation', 'level': 4, 'score': 0.41294533}, {'id': 'https://openalex.org/C96267730', 'wikidata': 'https://www.wikidata.org/wiki/Q4647010', 'display_name': 'A-site', 'level': 3, 'score': 0.41182357}, {'id': 'https://openalex.org/C8010536', 'wikidata': 'https://www.wikidata.org/wiki/Q160398', 'display_name': 'Crystallography', 'level': 1, 'score': 0.40315282}, {'id': 'https://openalex.org/C32909587', 'wikidata': 'https://www.wikidata.org/wiki/Q11369', 'display_name': 'Molecule', 'level': 2, 'score': 0.39958236}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.3490429}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.20019063}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.17289725}, {'id': 'https://openalex.org/C178790620', 'wikidata': 'https://www.wikidata.org/wiki/Q11351', 'display_name': 'Organic chemistry', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D017354', 'descriptor_name': 'Point Mutation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D020641', 'descriptor_name': 'Polymorphism, Single Nucleotide', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D015202', 'descriptor_name': 'Protein Engineering', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D014305', 'descriptor_name': 'Triose-Phosphate Isomerase', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D014305', 'descriptor_name': 'Triose-Phosphate Isomerase', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D000096', 'descriptor_name': 'Acetone', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000096', 'descriptor_name': 'Acetone', 'qualifier_ui': 'Q000031', 'qualifier_name': 'analogs & derivatives', 'is_major_topic': False}, {'descriptor_ui': 'D000096', 'descriptor_name': 'Acetone', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D002384', 'descriptor_name': 'Catalysis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019343', 'descriptor_name': 'Citric Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019343', 'descriptor_name': 'Citric Acid', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D019343', 'descriptor_name': 'Citric Acid', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D018360', 'descriptor_name': 'Crystallography, X-Ray', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018698', 'descriptor_name': 'Glutamic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008958', 'descriptor_name': 'Models, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D050505', 'descriptor_name': 'Mutant Proteins', 'qualifier_ui': 'Q000037', 'qualifier_name': 'antagonists & inhibitors', 'is_major_topic': False}, {'descriptor_ui': 'D050505', 'descriptor_name': 'Mutant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D050505', 'descriptor_name': 'Mutant Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D050505', 'descriptor_name': 'Mutant Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D050505', 'descriptor_name': 'Mutant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015202', 'descriptor_name': 'Protein Engineering', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014305', 'descriptor_name': 'Triose-Phosphate Isomerase', 'qualifier_ui': 'Q000037', 'qualifier_name': 'antagonists & inhibitors', 'is_major_topic': False}, {'descriptor_ui': 'D014305', 'descriptor_name': 'Triose-Phosphate Isomerase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D014305', 'descriptor_name': 'Triose-Phosphate Isomerase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1093/protein/gzn002', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S44492648', 'display_name': 'Protein Engineering Design and Selection', 'issn_l': '1741-0126', 'issn': ['1741-0126', '1741-0134'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/18239072', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'display_name': 'Clean water and sanitation', 'score': 0.59, 'id': 'https://metadata.un.org/sdg/6'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 36, 'referenced_works': ['https://openalex.org/W1502020096', 'https://openalex.org/W1558542256', 'https://openalex.org/W1574135748', 'https://openalex.org/W1678327261', 'https://openalex.org/W1971942119', 'https://openalex.org/W1974579447', 'https://openalex.org/W1985066480', 'https://openalex.org/W1988702615', 'https://openalex.org/W1996664166', 'https://openalex.org/W1997430881', 'https://openalex.org/W1998217927', 'https://openalex.org/W2000840258', 'https://openalex.org/W2001916175', 'https://openalex.org/W2019535338', 'https://openalex.org/W2034399259', 'https://openalex.org/W2046696671', 'https://openalex.org/W2047928199', 'https://openalex.org/W2052656018', 'https://openalex.org/W2059917692', 'https://openalex.org/W2070364411', 'https://openalex.org/W2070439048', 'https://openalex.org/W2074986801', 'https://openalex.org/W2096993581', 'https://openalex.org/W2097493124', 'https://openalex.org/W2103622571', 'https://openalex.org/W2104940225', 'https://openalex.org/W2112791315', 'https://openalex.org/W2135059712', 'https://openalex.org/W2138817605', 'https://openalex.org/W2140439679', 'https://openalex.org/W2143991598', 'https://openalex.org/W2151448506', 'https://openalex.org/W2159415657', 'https://openalex.org/W2159848960', 'https://openalex.org/W2161262895', 'https://openalex.org/W85707285'], 'related_works': ['https://openalex.org/W4390830021', 'https://openalex.org/W2397671206', 'https://openalex.org/W2138594732', 'https://openalex.org/W2061058223', 'https://openalex.org/W2039263150', 'https://openalex.org/W1988880849', 'https://openalex.org/W1980625821', 'https://openalex.org/W1974116801', 'https://openalex.org/W1973920394', 'https://openalex.org/W1933686656'], 'abstract_inverted_index': {'A': [0], 'monomeric': [1], 'variant': [2, 19, 45], 'of': [3, 46, 52, 65, 71, 85, 96, 116, 140, 188], 'triosephosphate': [4], 'isomerase': [5], '(TIM)': [6], 'with': [7, 133, 198], 'a': [8, 72, 183], 'new': [9, 200], 'engineered': [10], 'binding': [11, 23, 30, 69, 83, 187, 201], 'groove': [12], 'has': [13], 'been': [14, 26, 59], 'characterized': [15], 'further.': [16], 'In': [17], 'this': [18, 189], '(ml8bTIM),': [20], 'the': [21, 29, 43, 53, 62, 68, 82, 86, 93, 112, 117, 134, 137, 144, 158, 178, 199], 'phosphate': [22, 87], 'loop': [24], 'had': [25], 'shortened,': [27], 'causing': [28], 'site': [31, 57, 70, 84, 95, 114, 161], 'to': [32, 150, 169], 'be': [33, 151], 'much': [34], 'more': [35, 124], 'extended.': [36], 'Here,': [37], 'it': [38], 'is': [39, 90, 109, 120, 147, 154, 192], 'reported': [40], 'that': [41, 92, 111, 136, 157], 'in': [42, 128], 'V233A': [44], 'ml8bTIM': [47], '(A-TIM),': [48], 'three': [49], 'important': [50], 'properties': [51, 64], 'wild-type': [54, 129, 170], 'TIM': [55, 100, 145, 171, 180], 'active': [56, 94, 113, 160], 'have': [58], 'restored:': [60], '(i)': [61], 'structural': [63], 'loop-7,': [66], '(ii)': [67], 'conserved': [73], 'water': [74], 'molecule': [75, 191], 'between': [76], 'loop-7': [77], 'and': [78, 80, 104, 123, 172], 'loop-8': [79], '(iii)': [81], 'moiety.': [88], 'It': [89, 108, 153], 'shown': [91, 156], 'A-TIM': [97, 118, 141, 159], 'can': [98, 162], 'bind': [99, 163, 168], 'transition': [101], 'state': [102], 'analogs': [103], 'suicide': [105], 'inhibitors': [106], 'competently.': [107], 'found': [110], 'geometry': [115], 'complexes': [119], 'less': [121], 'compact': [122], 'solvent': [125], 'exposed,': [126], 'as': [127], 'TIM.': [130], 'This': [131], 'correlates': [132], 'observation': [135], 'catalytic': [138], 'efficiency': [139], 'for': [142], 'interconverting': [143], 'substrates': [146], 'too': [148], 'low': [149], 'detected.': [152], 'also': [155], 'compounds': [164], 'which': [165, 173], 'do': [166], 'not': [167], 'are': [174], 'completely': [175], 'different': [176], 'from': [177], 'normal': [179], 'substrate,': [181], 'like': [182], 'citrate': [184, 190], 'molecule.': [185], 'The': [186], 'stabilized': [193], 'by': [194], 'hydrogen': [195], 'bonding': [196], 'interactions': [197], 'groove.': [202]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2103687648', 'counts_by_year': [{'year': 2020, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-11T05:29:37.412533', 'created_date': '2016-06-24'}