TSG101/Mammalian VPS23 and Mammalian VPS28 Interact Directly and Are Recruited to VPS4-induced Endosomes

Name: Work Video:
Duration: 3 min 30 s
Naomi Bishop; Philip Woodman
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3883, 3974, 4008, 4054, 4340], 'TSG101/mammalian': [47, 182], 'VPS23': [48, 183], 'to': [49, 115, 184, 250, 290, 294, 340, 374, 573, 597, 735, 792, 797, 849, 859, 1113, 1119, 1124, 1136, 1200, 1260, 1289, 1305, 1362, 1421, 1522, 1589, 1695, 1813, 1842, 2062, 2074, 2128, 2155, 2220, 2452, 2575, 2618, 2668, 2929, 2972, 2980, 3008, 3088, 3243, 3252, 3277, 3578, 3647, 3818, 3900, 3908, 3977, 4052, 4091, 4094, 4135, 4216, 4236, 4248, 4261, 4296, 4338, 4358, 4370], 'form': [50, 185, 1290, 3244, 4371], 'part': [51, 186, 1325, 4277], 'multiprotein': [54, 189, 868, 3245], 'complex.': [55, 190], 'Co-immunoprecipitation': [56, 191], 'cross-linking': [58, 193, 2671, 3412, 3450, 3513, 3710], 'experiments': [59, 194, 3182], 'demonstrated': [60, 195], 'hVPS28': [62, 82, 113, 197, 217, 248, 1358, 1454, 1619, 1678, 1697, 1738, 1752, 2131, 2226, 2828, 2910, 3010, 3020, 3069, 3086, 3103, 3117, 3128, 3158, 3250, 3260, 3279, 3333, 3425, 3644, 3655, 3668, 3734, 3789, 3928], 'TSG101': [64, 80, 111, 124, 199, 215, 246, 259, 1122, 1130, 1199, 1208, 1256, 1277, 1356, 1381, 1774, 1851, 3281, 3374, 3427, 3642, 3653, 3666, 3754, 3794, 3806, 3834, 3849, 3875, 3897, 3926, 4125, 4231, 4252, 4262, 4339, 4359, 4366], 'interact': [65, 200, 1279, 3239, 4053], 'directly': [66, 130, 201, 265, 1351, 2123, 3646], 'binding': [69, 204, 796], 'requires': [70, 205], 'structural': [71, 206, 668, 1318], 'information': [72, 207], 'conserved': [75, 210, 4300], 'C-terminal': [76, 211, 1824, 4349, 4363], 'portion': [77, 108, 212, 243, 4288, 4364], 'TSG101.': [79, 214, 1353], 'predominantly': [84, 219], 'cytosolic.': [85, 220, 3110], 'However,': [86, 221, 1156, 3193, 3346, 3535, 3709], 'when': [87, 222, 2290, 3055, 3341], 'endosomal': [88, 133, 223, 268, 447, 801, 1274, 3916], 'vacuolization': [89, 224], 'was': [90, 225, 978, 1133, 1390, 1401, 1419, 1439, 1447, 1467, 1507, 1514, 1556, 1598, 1607, 1679, 1753, 1777, 1788, 1804, 1810, 1830, 1869, 1883, 2056, 2153, 2539, 2553, 2616, 2657, 2795, 2826, 3003, 3032, 3095, 3108, 3142, 3151, 3261, 3280, 3398, 3401, 3414, 3419, 3538, 3605, 3622, 3669, 3743, 3790, 3876, 3935, 4089, 4192, 4214], 'induced': [91, 226], 'by': [92, 227, 400, 455, 566, 986, 1264, 1366, 1461, 1527, 1538, 1764, 1856, 2099, 2239, 2384, 2391, 2405, 2423, 2432, 2529, 2541, 2987, 3118, 3358, 3388, 3404, 3581, 3745, 3751, 3856], 'expression': [94, 229, 1267, 1684, 1815, 2209, 2303, 3083], 'dominant-negative': [97, 232, 1370], 'mutant': [98, 233, 1371], 'another': [100, 235, 927], 'class': [101, 236, 394, 426, 466, 593, 622, 650, 673, 863, 928, 1285, 1338, 3235, 3255, 3268], 'vps': [103, 238, 363, 396, 428, 675, 930, 1287, 1340, 3237, 3257], 'protein,': [104, 239, 1341], 'human': [105, 240, 1031, 1347, 1542, 1614, 2050, 2816, 3036, 4095, 4228], 'VPS4,': [106, 241, 1373], 'both': [110, 245, 1355, 3704], 'translocated': [114, 249], 'surface': [117, 252, 271, 293, 942, 1296], 'these': [119, 254, 335, 1057, 1111, 1734, 3638, 3722, 4190], 'vacuoles.': [120, 255], 'conclude': [122, 257], 'its': [126, 261, 789, 1006, 2237, 3275], 'interacting': [127, 262], 'components': [128, 263, 865, 3406, 3913], 'involved': [131, 266, 1272, 3914], 'sorting.': [134, 269, 362, 878, 1385], 'Cell': [270, 322, 496, 538, 2567, 3461, 3487], 'internalized': [274], 'vesicles': [277], 'enter': [279], 'early': [281, 1363, 3220], 'endosome,': [282], 'where': [283], 'they': [284, 440], 'sorted.': [286], 'Many': [287], 'return': [289], 'cell': [292, 941, 2434, 3855, 3880], 'engage': [295], 'further': [297, 643, 875, 1337, 2913, 3728, 4128], 'rounds': [298, 876], 'ligand': [300], 'uptake,': [301], 'whereas': [302], 'other': [303, 861, 1284, 2833, 2947, 3242, 3253, 3267, 3649, 3719, 4238], 'targeted': [306], 'via': [307, 1893], 'body': [310], '(MVB)1/late': [311], 'endosome': [312, 1364], 'degradation': [314, 939, 1263], 'lysosome': [317], '(1Mellman': [318], 'I.': [319, 411, 704, 827], 'Ann.': [320], 'Rev.': [321], 'Dev.': [323], 'Biol...': [324, 497, 539, 552, 588, 909, 959, 1933, 1946, 1997, 2568, 3308, 3462, 3488], '1996;': [325, 529, 553, 589, 1001, 1038, 1152, 1571, 1662, 1920, 1959, 2009, 2899, 3287, 3489, 3961], '12:': [326], '575-625Google': [327], 'Scholar).': [328, 391, 422, 556, 641, 840, 976, 1097, 1155, 1192, 1254, 1499, 2048, 2608, 3325, 3492, 3964, 4002, 4084, 4324], 'The': [329, 465, 1129, 1500, 1582, 1595, 1674, 1785, 1807, 1867, 2081, 2125, 2170, 2198, 2228, 2386, 2535, 2755, 2783, 2934, 3019, 3210, 3595, 3686, 3931, 4003, 4185, 4224, 4287, 4352, 4362], 'molecular': [330, 3511, 3544, 3598, 3688, 3763, 3835], 'basis': [331], 'selection': [333, 1409], 'their': [338, 571, 2093], 'transfer': [339], 'MVB': [342, 630], 'remains': [343, 3846], 'poorly': [344], 'characterized.': [345], 'Studies': [346], 'have,': [349], 'however,': [350], 'number': [353, 665, 1017, 1282, 1624, 2793, 2823, 4270], 'gene': [355, 985, 994, 1012, 1787, 2132, 2168, 3933, 4179], 'products': [356, 2085, 2230, 2242, 2251, 2677, 3711], 'post-endosomal': [361], '(vacuolar': [364], 'proteinsorting)': [365], 'mutants': [366, 397, 429], 'defective': [368], 'delivering': [370], 'newly': [371], 'synthesized': [372], 'hydrolases': [373, 478], 'vacuole': [376, 606], '(the': [377], 'lysosome)': [379], '(2Bryant': [380], 'N.J.': [381, 547, 583], 'Stevens': [382, 414, 493, 548, 584], 'T.H.': [383, 415, 494, 549, 585], 'Microbiol.': [384], 'Mol.': [385, 416, 526, 550, 586, 682, 711, 807, 834, 907, 957, 1177, 1932, 1945, 1996, 2602, 2896, 3306, 3987], 'Biol.': [386, 417, 527, 683, 712, 808, 835, 2603, 2897], 'Rev...': [387], '1998;': [388, 638, 759, 1094, 1235, 2605, 4029], '62:': [389], '230-247Google': [390], 'Among': [392, 3271], 'these,': [393], 'characterized': [399], 'formation': [402, 628, 3702], 'an': [404, 987, 1699, 1819, 1846, 2878, 2917, 2950, 3364, 3381, 3761, 3971, 4218, 4372], 'exaggerated': [405], 'compartment': [407, 468], '(3Raymond': [408], 'C.K.': [409], 'Howald-Stevenson': [410], 'Vater': [412], 'C.A.': [413, 781], 'Cell..': [418, 528, 637, 684, 713, 809, 836, 1000, 1037, 1151, 2604, 2898, 3286, 3960], '1992;': [419], '3:': [420], '1389-1402Google': [421], 'At': [423, 3162], 'least': [424, 2681], '15': [425, 2441, 2648], 'been': [431, 437, 452, 1060, 1165, 2204, 3430, 4182], 'yeast.': [434], 'It': [435, 3845, 3902], 'has': [436, 451, 1059, 1163, 4180, 4367], 'proposed': [438], 'act': [441, 1210, 1258], 'at': [442, 1706, 1717, 1822, 1832, 2243, 2324, 2410, 2436, 2580, 2680, 2737, 2767, 2799, 3091, 3376], 'common': [444], 'step': [445], 'sorting,': [448, 3917], 'this': [450, 880, 3200, 3396, 4131, 4330, 4356], 'borne': [453], 'out': [454], 'several': [456, 2212], 'studies': [457, 1019, 1328], 'demonstrating': [458, 4111], 'physical': [459], 'and/or': [460], 'genetic': [461], 'interactions': [462, 886], 'between': [463, 2940, 3366, 3837, 4187], 'them.': [464], 'contains': [469, 3911], 'endocytosed': [470], 'markers,': [471], 'well': [473], 'precursors': [475], 'markers': [480, 2259, 3218], 'normally': [481], 'associated': [482, 1102], 'trans': [485, 564], 'Golgi': [486, 565], '(4Piper': [487], 'R.C.': [488], 'Cooper': [489], 'A.A.': [490], 'Yang': [491], 'H.': [492, 536, 775, 1226, 4020], 'J.': [495, 537, 1079, 1081, 1176, 1228, 1481, 1926, 1931, 1941, 1944, 1952, 1995, 2019, 2595, 3485, 3986, 4022], '1995;': [498, 2045, 4081], '131:': [499], '603-617Google': [500], 'Scholar,': [501, 515, 532, 543, 813, 913, 963, 1041, 1075, 1182, 1238, 1653, 1937, 1950, 1962, 2037, 3312, 3466, 3483, 3992, 4032, 4067], '5Babst': [502], 'M.': [503, 634, 706, 708, 750, 829, 831, 915, 965, 1043, 1077, 1218, 1567, 2563, 3314, 3457, 3773, 4012, 4099], 'Sato': [504], 'T.K.': [505], 'Banta': [506, 518, 2888], 'L.M.': [507, 519, 2889], 'Emr': [508, 524, 635, 755, 920, 970, 2894, 3319, 3778, 4104], 'S.D.': [509, 525, 636, 756, 921, 971, 2895, 3320, 3779, 4105], 'EMBO': [510, 757, 1249, 4043], 'J...': [511, 758, 1250, 4044], '1997;': [512, 1072, 1179, 1189, 3989, 3999, 4321], '16:': [513, 554, 590, 1073, 1190, 1973, 4000, 4065, 4322], '1820-1831Google': [514], '6Rieder': [516], 'S.E.': [517, 2887], 'Köhrer': [520, 2890], 'K.': [521, 2891], 'McCaffery': [522, 2892], 'J.M.': [523, 2015, 2893], '7:': [530, 2035, 2900], '985-999Google': [531, 2901], '7Munn': [533], 'A.L.': [534], 'Riezman': [535], '1994;': [540, 784, 1650, 2034, 4207], '127:': [541], '373-386Google': [542], '8Nothwehr': [544], 'S.F.': [545, 581], 'Bryant': [546, 582], 'Cell.': [551, 587, 908, 958, 3307], '2700-2707Google': [555, 591], 'Because': [557], 'retained': [560], 'mechanism': [568], 'involves': [570], 'transport': [572], 'retrieval': [575], 'from': [576, 608, 800, 866, 1440, 1546, 1558, 1729, 1779, 2511, 3176, 3230, 3740, 3879], 'pathway': [579], '(8Nothwehr': [580], 'Scholar),': [592, 688, 717, 762, 926, 1004, 1052, 1665, 1912, 1923, 1975, 1989, 2001, 2012, 2025, 3290, 3784, 4110, 4210], 'appear': [596], 'be': [598, 850, 1360, 2122, 3336, 4092], 'important': [599], 'material': [602], 'bound': [603, 2713], 'away': [607], 'cycle': [611, 794, 2108], 'through': [612, 2395], 'system.': [615, 2479], 'Indeed,': [616], 'recent': [617, 1195], 'data': [618, 853, 2808, 2837, 2996, 3723], 'consistent': [620, 855, 1374, 3700, 3768, 3821], 'participating': [625], '(9Odorizzi': [631], 'G.': [632, 917, 967, 1083, 1085, 1904, 1970, 1984, 2031, 2041, 3316, 3775, 4101], 'Babst': [633], '95:': [639], '847-858Google': [640], 'To': [642, 3073, 3247, 3393, 3726], 'our': [644, 852, 1327], 'understanding': [645], 'on': [653, 1294, 2314, 2473, 2643, 2751, 2806, 2835, 3715], 'we': [658, 882, 1329, 3164, 3919, 4145], 'generated': [660], 'specific': [661, 1030, 1269], 'reagents': [662], 'close': [667], 'homologues': [670, 2841], 'proteins.': [676, 3270, 3368], '(10Bishop': [678, 803], 'N.': [679, 698, 804, 821, 2597], 'Woodman': [680, 805, 2600], 'P.G.': [681, 806], '2000;': [685, 714, 810, 837, 923, 973, 1972, 3322, 3781, 4107], '11:': [686, 715, 811, 838, 2046], '227-239Google': [687, 812], 'others': [690], '(11Yoshimori': [691], 'T.': [692, 815, 900, 950, 1224, 1485, 3299, 4018], 'Yamagata': [693, 816], 'F.': [694, 771, 817], 'Yamamoto': [695, 818], 'A.': [696, 702, 819, 825, 1866, 4060, 4069, 4073, 4164], 'Mizushima': [697, 820], 'Kabeya': [699, 822], 'Y.': [700, 710, 823, 833, 898, 948, 1220, 1222, 1978, 3297, 4014, 4016], 'Nara': [701, 824], 'Miwako': [703, 826], 'Ohashi': [705, 828], 'Ohsumi': [707, 709, 830, 832], '747-763Google': [716, 839], 'shown': [719, 3098, 3327], 'orthologues': [722, 4136, 4149], 'AAA': [725], '(ATPases': [726], 'Associated': [727], 'cellular': [729, 990, 3076, 3896], 'Activities)-type': [730], 'ATPase,': [731], 'Vps4p,': [732], 'maintain': [736], 'morphological': [738], 'functional': [740, 4353], 'organization': [741], 'pathway.': [745], 'Like': [746], 'Vps4p': [748], '(12Babst': [749], 'Wendland': [751], 'B.': [752, 1906, 1930, 1943, 1956, 1992, 2017], 'Estapa': [753], 'E.J.': [754, 919, 969, 3318, 3777, 4103], '17:': [760], '2982-2993Google': [761], '(m)VPS4': [764], '(and': [765], 'closely': [767, 2927, 4234, 4259], 'related': [768, 2163, 2928, 4235, 4260], 'SKD1': [769], '(13Périer': [770], 'Coulter': [772], 'K.L.': [773], 'Liang': [774], 'Radeke': [776], 'C.M.': [777], 'Gaber': [778], 'R.F.': [779], 'Vandenberg': [780], 'FEBS': [782, 2020], 'Lett...': [783, 2021], '351:': [785], '286-290Google': [786], 'Scholar))': [787], 'couples': [788], 'ATPase': [790], 'activity': [791], 'release': [799, 860], 'membranes': [802, 1365], '11Yoshimori': [814], 'Although': [841, 1098, 3811], 'immediate': [843], 'substrates': [844], 'mVPS4': [846, 857], 'yet': [848], 'identified,': [851], 'acting': [858], 'soluble': [862], 'endosome-associated': [867], 'complex(es)': [869], 'thus': [871], 'recycle': [872], 'them': [873], 'In': [879, 2501, 4133], 'study': [881], 'examined': [884, 2336, 3038], 'TSG101,': [888, 932], 'previously': [889, 2557], 'Vps23p/Stp22p': [896, 3295], '(14Li': [897, 947, 3296], 'Kane': [899, 949, 3298], 'Tipper': [901, 951, 3300], 'C.': [902, 952, 1565, 1781, 1964, 1966, 1968, 1980, 1994, 2029, 2039, 3301, 4151, 4249], 'Spatrick': [903, 953, 3302], 'P.': [904, 954, 1063, 1175, 1982, 2006, 2601, 3303, 3470, 3985], 'Jenness': [905, 955, 3304], 'D.D.': [906, 956, 3305], '1999;': [910, 960, 1049, 1251, 1986, 3309, 4045], '19:': [911, 961, 3310], '3588-3599Google': [912, 962, 3311], '15Babst': [914, 964, 3313], 'Odorizzi': [916, 966, 3315, 3774, 4100], 'Estepa': [918, 968, 3317, 3776, 4102], 'Traffic..': [922, 972, 3321, 3780, 4106], '1:': [924, 974, 1910, 3323, 3782, 4108], '248-258Google': [925, 975, 3324, 3783, 4109], 'protein.': [931, 2962, 4132], 'like': [933], 'Vps23p/Stp22p,': [934], 'positively': [935], 'regulates': [936], 'lysosomal': [938], 'proteins,': [943, 3258, 4305], 'including': [944], 'mitogenic': [945, 1126], 'Tsg101': [977], 'originally': [980, 3936], 'tumor': [983, 1010], 'susceptibility': [984, 1109], 'assay': [988], 'transformation': [991], 'upon': [992, 3517, 3550], 'random': [993], 'disruption': [995], '(16Li': [996, 1033, 1147, 3282, 3956], 'L.': [997, 1034, 1148, 3283, 3957], 'Cohen': [998, 1035, 1149, 3284, 3958], 'S.N.': [999, 1036, 1150, 3285, 3959], '85:': [1002, 1039, 1153, 3288, 3962], '319-329Google': [1003, 1040, 1154, 3289, 3963], 'although': [1005, 1053, 4171], 'importance': [1007], 'suppressor': [1011, 1216], 'is': [1013, 1117, 2925, 2949, 3022, 3190, 3195, 3202, 3864, 3898, 3903, 4050, 4114, 4263, 4294, 4360], 'still': [1014, 1797], 'debated.': [1015], 'A': [1016, 1488, 1541, 2049, 2147, 2998, 4257], 'reported': [1021], 'presence': [1023, 3436, 3826, 3969], 'aberrant': [1025], 'splice': [1026, 3066], 'variants': [1027, 3051, 3067], 'ofTSG101': [1028], 'tumors': [1032], '17Ferrer': [1042], 'López-Borges': [1044], 'S.': [1045, 1230, 2599, 2941, 3478, 4024, 4079, 4143, 4177], 'Lazo': [1046], 'P.A.': [1047], 'Oncogene..': [1048], '18:': [1050, 1252, 4046], '2253-2259Google': [1051], 'significance': [1055], 'findings': [1058, 3771], 'disputed': [1061], '(18Steiner': [1062], 'Barnes': [1064], 'D.M.': [1065], 'Harris': [1066], 'W.H.': [1067], 'Weinberg': [1068], 'R.A.': [1069, 1186, 3996, 4318], 'Nat.': [1070, 1187, 3997, 4319], 'Genet...': [1071, 1188, 3998, 4320], '332-333Google': [1074], '19Hampl': [1076], 'Hempl': [1078], 'Plaschke': [1080], 'Fitze': [1082], 'Scheckert': [1084], 'Saeger': [1086], 'H.D.': [1087], 'Schackert': [1088], 'H.K.': [1089], 'Biochem.': [1090, 1231, 4025, 4062], 'Biophys.': [1091, 1232, 4026], 'Res.': [1092, 1233, 4027], 'Commun...': [1093, 1234, 4028], '248:': [1095], '753-760Google': [1096], 'defect': [1101], 'TSG101-deficient': [1104], 'cells': [1105, 1112, 2263, 2287, 2347, 2366, 2388, 3107, 3134, 3742], 'could': [1106, 1257, 2121, 3334], 'underlie': [1107], 'transformation,': [1114], 'limited': [1115], 'evidence': [1116, 1206, 3362, 3662, 3729, 3923], 'available': [1118], 'explain': [1120], 'how': [1121], 'acts': [1123], 'regulate': [1125], 'receptor': [1127, 1262, 1384], 'down-regulation.': [1128], 'peptide': [1131], 'sequence': [1132, 1584, 1627, 2785, 2881, 2907, 2919, 2939, 2983, 4122], 'first': [1134, 4276, 4308], 'interpreted': [1135], 'contain': [1137, 3579], 'two': [1138, 1682, 3542], 'DNA-binding': [1139, 1161], 'motifs,': [1140], 'perhaps': [1141], 'indicative': [1142], 'transcription': [1145], 'factor': [1146], 'existence': [1158], 'motifs': [1162, 2984], 'not': [1164, 3063, 3071, 3166, 3184, 3215, 3227, 3335, 3391, 3402, 3619, 3658, 3684, 3894, 4181], 'substantiated': [1166], 'subsequent': [1168], 'analyses': [1169, 4118], '(20Ponting': [1170, 3980], 'C.P.': [1171, 3981], 'Cai': [1172, 3982], 'Y.-D.': [1173, 3983], 'Bork': [1174, 3984], 'Med...': [1178, 3988], '75:': [1180, 3990], '467-469Google': [1181, 3991], '21Koonin': [1183, 3993], 'E.V.': [1184, 3994, 4316], 'Abagyan': [1185, 3995, 4317], '330-331Google': [1191, 4001, 4323], 'Notwithstanding': [1193], 'this,': [1194], 'reports': [1196], 'linked': [1198], 'transcriptional': [1201, 1215], 'regulation': [1202], 'provided': [1205], 'can': [1209, 1359, 3929], 'vitro': [1212, 3432], '(22Watanabe': [1217, 4011], 'Yanagi': [1219, 4013], 'Masuhiro': [1221, 4015], 'Yano': [1223, 4017], 'Yoshikawa': [1225, 4019], 'Yanagisawa': [1227, 4021], 'Kato': [1229, 4023], '245:': [1236, 4030], '900-905Google': [1237, 4031], '23Hittelman': [1239, 4033], 'A.B.': [1240, 4034], 'Burakov': [1241, 4035], 'D.': [1242, 2004, 2486, 2559, 2932, 3453, 4036, 4267], 'Iniguez-Lluhı́': [1243, 4037], 'J.A.': [1244, 4038], 'Freedman': [1245, 4039], 'L.P.': [1246, 4040], 'Garabedian': [1247, 4041], 'M.J.': [1248, 1917, 4042], '5380-5388Google': [1253, 4047], 'Therefore,': [1255], 'indirectly': [1259], 'aid': [1261], 'modulating': [1265], 'protein(s),': [1270], 'themselves': [1271], 'trafficking.': [1275], 'Alternatively,': [1276], 'might': [1278, 1303], 'complex': [1293, 1382, 3807, 3871, 3910], 'endosome.': [1300], 'Here,': [1301, 3416], 'it': [1302, 1606, 3194], 'recognize': [1306], 'cargo': [1307], 'destined': [1308], 'inclusion': [1310], 'body,': [1314], 'or': [1315, 1415, 1435, 1526, 1714, 1724, 1742, 2994, 3224, 3422, 3426, 3497, 3625, 3667, 3859, 3927], 'provide': [1316, 3727], 'component': [1319], 'event.': [1323], 'As': [1324, 3097, 3326], 'Vps28p.': [1342], 'now': [1344], 'demonstrate': [1345, 3394], 'VPS28': [1348, 2871, 2924, 3141, 3173, 3189, 3212], '(hVPS28)': [1349], 'Moreover,': [1354, 3797], 'recruited': [1361], 'co-expression': [1367], 'direct': [1377, 3399], 'role': [1378], 'Escherichia': [1386], 'colistrain': [1387], 'XL1Blue': [1388], '(Stratagene)': [1389, 1519], 'used': [1391, 1402, 1455, 1479, 1508, 2127, 2154, 2202, 2574], 'maintenance,': [1393], 'sequencing,': [1394, 1511, 1604], 'mutation': [1396], 'plasmids.': [1398], 'Luria-Bertani': [1399], 'medium': [1400, 2271, 2351], 'growth': [1404], 'ofE.': [1405], 'coli': [1406], 'cells.': [1407], 'For': [1408, 1749, 2284, 2342, 2425, 2545, 2609, 2670, 2705], 'plasmids,': [1411], '100': [1412, 2465, 2629], 'μg/ml': [1413, 1417], 'ampicillin': [1414], '25': [1416, 2587], 'kanamycin': [1418], 'added': [1420], 'media.': [1423], 'Proteins': [1424, 2480], 'tagged': [1425, 3375], 'withmyc/His6': [1426], 'were': [1427, 1459, 1478, 1536, 1629, 1668, 1693, 1727, 1736, 1854, 2086, 2133, 2175, 2231, 2252, 2264, 2288, 2310, 2317, 2335, 2348, 2367, 2389, 2403, 2421, 2430, 2450, 2481, 2503, 2527, 2678, 2701, 2715, 2749, 2758, 2773, 2777, 2985, 3052, 3494, 3515, 3548, 3562, 3576, 3601, 3676], 'detected': [1428, 3033, 3053, 3549], 'using': [1429, 1469, 1516, 1631, 1670, 1871, 1885, 2058, 2066, 2206, 2293, 2322, 2329, 2337, 2359, 2483, 2505, 2688, 3005, 4194], 'mouse': [1430, 1773, 4230], 'monoclonal': [1431], 'antibody': [1432, 1446, 2763, 3113, 3137, 3340, 3585, 3682, 3886], '9E10': [1433], '(Sigma)': [1434], 'anti-polyhistidine': [1436], '(Sigma).': [1437], 'Anti-TSG101': [1438], 'AbCam,': [1441], 'Cambridge,': [1442, 2697], 'UK.': [1443], 'An': [1444, 1826, 3111], 'anti-hVPS28': [1445, 3885], 'raised': [1448], 'sheep,': [1450], 'His6-tagged': [1452, 3332], 'recombinant': [1453, 1475], 'antigen.': [1457], 'Immunizations': [1458], 'performed': [1460, 1515, 1630, 1669, 1855, 2504, 2702, 4215], 'Diagnostics': [1462], 'Scotland': [1463], '(Carluke,': [1464], 'Lanarkshire).': [1465], 'Antibody': [1466], 'affinity-purified': [1468, 3112], 'glutathioneS-transferase-tagged': [1470], 'hVPS28.': [1471, 3901], 'Standard': [1472], 'protocols': [1473], 'DNA': [1476, 1510, 1539, 1596, 1868, 2257, 2520], 'manipulation': [1477], '(24Sambrook': [1480], 'Fritsch': [1482], 'E.F.': [1483], 'Maniatis': [1484], 'Molecular': [1486, 2299], 'Cloning:': [1487], 'Laboratory': [1489], 'Manual.': [1490], 'Cold': [1491, 1495], 'Spring': [1492, 1496], 'Harbor': [1493], 'Laboratory,': [1494], 'Harbor,': [1497], 'NY1989Google': [1498], 'ABI': [1501], 'PRISM': [1502], 'big': [1503], 'dye': [1504], 'terminator': [1505], 'system': [1506], 'mutagenesis': [1513, 1529], 'QuikChange': [1518], 'method': [1520, 2297], 'according': [1521, 2061], "manufacturer's": [1524, 2064], 'instructions,': [1525, 2065], 'cassette': [1528], '(see': [1530, 4284, 4377], 'below).': [1531], 'Mutagenesis': [1532], 'subcloning': [1534], 'constructs': [1535], 'confirmed': [1537, 2540, 3577], 'sequencing.': [1540], 'cDNA': [1543, 1611, 1675, 1776, 2053, 2813, 3001], 'EST': [1544, 1583, 2788], 'clone': [1545, 2789], 'Soares': [1548, 1568], 'senescent': [1549], 'fibroblast': [1550], '(NbHSF)': [1551], 'library': [1552], '(Clone': [1553], 'ID': [1554], '1331807)': [1555], 'obtained': [1557, 1778, 3563, 3670], 'IMAGE': [1560], 'consortium': [1561], '(25Lennon': [1562], 'G.G.': [1563], 'Auffray': [1564], 'Polymeropoulos': [1566], 'M.B.': [1569], 'Genomics..': [1570], '33:': [1572], '151-152Google': [1573], 'Scholar)': [1574, 2494, 2572, 2902, 4048], 'vector': [1576, 1839, 3084], 'pT7T3D,': [1577], 'modified': [1580, 2268, 2273], 'polylinker.': [1581], 'had': [1585, 2203, 3429], 'similarity': [1588, 2920], '5′-end': [1591], 'VPS28.': [1594, 3180], 'insert': [1597, 1676, 2306], 'subcloned': [1599, 1680, 1754, 1790], 'into': [1600, 1681, 1755, 1791], 'pBluescript': [1601, 1768], 'SKII(−)': [1602, 1769], 'found': [1608, 2846, 3921, 4090, 4147, 4265], 'encoded': [1612, 2814], 'homologue': [1615, 2817], 'yeastVPS28,': [1617], 'denoted': [1618], '(human': [1620], 'VPS28;': [1621], 'GenBank™': [1622], 'accession': [1623, 2792, 2822], 'AF316887).': [1625], 'Multiple': [1626], 'alignments': [1628], 'BCM': [1633], 'search': [1634], 'launcher': [1635], 'ClustalW': [1638, 4196], '1.8': [1639], 'program': [1640, 4197], '(26Thompson': [1641, 4198], 'J.D.': [1642, 1657, 4199], 'Higgins': [1643, 4200], 'D.G.': [1644, 1655, 4201], 'Gibson': [1645, 1658, 4202], 'T.J.': [1646, 1659, 4203], 'Nucleic': [1647, 4204], 'Acids': [1648, 4205], 'Res...': [1649, 4206], '22:': [1651, 4208], '4673-4680Google': [1652, 4209], '27Higgins': [1654], 'Thompson': [1656], 'Methods': [1660, 1957, 2566, 3460], 'Enzymol...': [1661, 1958], '266:': [1663, 1960], '383-402Google': [1664], 'graphics': [1667], 'BOXSHADE': [1672], 'server.': [1673, 1899], 'vectors': [1685, 1692, 1735, 1763, 2304], 'pEGFPC': [1686], '(CLONTECH)': [1687, 2055], 'pcDNA3.1myc/His': [1689], '(Invitrogen).': [1690], 'These': [1691, 1900, 2963], 'designed': [1694, 1841], 'express': [1696, 1843], 'enhanced': [1700], 'green': [1701], 'fluorescent': [1702], '(eGFP)': [1704], 'tag': [1705, 1716, 1744, 1829, 3090], 'N': [1708, 1834, 3378], 'terminus': [1709, 1720, 1835, 3094, 3379], '(hVPS28-pEGFP)': [1713], 'amyc/His6': [1715, 3089], 'C': [1719, 3093], '(hVPS28-pcDNA3.1-myc/His).': [1721], 'Subsequently,': [1722], 'themyc': [1723], 'His6': [1725, 1741], 'epitopes': [1726], 'deleted': [1728, 1812], 'construct': [1730], 'hVPS28-pcDNA3.1-myc/His,': [1731], 'and,': [1732], 'therefore,': [1733], 'expressed': [1737, 3104, 3127, 3157, 3179], 'myc': [1743], 'alone': [1745, 2309, 3343, 3521, 3624], '(hVPS28-pcDNA3.1-His': [1746], 'hVPS28-pcDNA3.1-myc).': [1748], 'prokaryotic': [1750], 'expression,': [1751], 'pGEX': [1756], '(Amersham': [1757], 'Pharmacia': [1758, 2476], 'Biotech)': [1759], 'pTrcHis': [1761], '(Invitrogen)': [1762], 'standard': [1765, 2506], 'methods.': [1766], 'Plasmid': [1767], 'containing': [1770, 2279, 2352, 2636, 2674, 2712, 3085], 'full-length': [1772], '(mTSG101)': [1775], 'Dr.': [1780], 'Miller': [1782], '(Stanford': [1783], 'University).': [1784], 'mTSG101': [1786, 1817, 1844], 'subsequently': [1789], 'pcDNA3.1myc/His,': [1792], 'stop': [1795, 1808], 'codon': [1796, 1809, 4174], 'present': [1798], 'before': [1799, 2089, 2233], 'myc/His6': [1801], 'tag,': [1802], 'named': [1805], 'mTSG101-pcDNA3.1.': [1806], 'then': [1811, 1878, 4244], 'allow': [1814], 'in-frame': [1820], 'myc/His6tag': [1821], 'end.': [1825], 'HA': [1827, 1848], 'epitope': [1828, 1849], 'introduced': [1831], 'mTSG101-pcDNA3.1,': [1837], 'creating': [1838], 'HA-mTSG101-pcDNA3.1,': [1840], 'N-terminal': [1847, 3972, 4309], 'tag.': [1850], 'domain': [1852, 3943, 4006, 4283, 4292, 4336, 4376], 'deletions': [1853], 'introducing': [1857], 'restriction': [1858, 1875], 'enzyme': [1859], 'sites': [1860], 'indicated': [1862, 2810, 2839], 'Fig.': [1864, 3100, 3329, 4285, 4378], '4': [1865, 2765, 2768, 4169, 4222, 4379], 'cut': [1870], 'pair-wise': [1872], 'combinations': [1873], 'enzymes,': [1876], 'religated.': [1879], 'Secondary': [1880], 'structure': [1881, 1890, 4337], 'prediction': [1882, 1891], 'determined': [1884, 2098], 'variety': [1887], 'secondary': [1889, 2706], 'methods': [1892], 'Network': [1895], 'Protein': [1896, 1907, 1918, 2007, 2032, 2770], 'Sequence': [1897, 2797], 'Analysis': [1898], 'included': [1901], 'DPM': [1902], '(28Deleage': [1903], 'Roux': [1905], 'Eng...': [1908, 2008, 2033], '1987;': [1909, 1947], '289-294Google': [1911], 'DSC': [1913], '(29King': [1914], 'R.D.': [1915], 'Sternberg': [1916], 'Sci...': [1919, 4063], '5:': [1921], '2298-2310Google': [1922], 'GOR': [1924], '(30Garnier': [1925], 'Osguthorpe': [1927], 'D.J.': [1928], 'Robson': [1929, 1942, 1955, 2016], '1978;': [1934], '120:': [1935], '97-120Google': [1936], '31Gibrat': [1938], 'J.F.': [1939], 'Garnier': [1940, 2018], '198:': [1948], '425-443Google': [1949], '32Garnier': [1951], 'Gibrat': [1953], 'J.-F.': [1954], '540-553Google': [1961], '33Blanchet': [1963], 'Combet': [1965], 'Geourjon': [1967, 1979], 'Deleage': [1969, 1983, 2030, 2040], 'Bioinformatics..': [1971, 1985], '286-287Google': [1974], 'MLRC': [1976], '(34Guermeur': [1977], 'Gallinari': [1981], '15:': [1987], '413-421Google': [1988], 'PHD': [1990], '(35Rost': [1991], 'Sander': [1993], '1993;': [1998], '232:': [1999], '584-599Google': [2000], 'Predator': [2002], '(36Frishman': [2003], 'Argos': [2005], '9:': [2010, 2606], '133-142Google': [2011], 'SIMPA96': [2013], '(37Levin': [2014], '1986;': [2022, 3480], '205:': [2023], '303-308Google': [2024], 'SOPM': [2027], '(38Geourjon': [2028], '157-164Google': [2036], '39Geourjon': [2038], 'Comput.': [2042], 'Appl.': [2043], 'Biosci...': [2044], '681-684Google': [2047], 'multiple': [2051, 2199, 2999], 'tissue': [2052, 2120, 2200, 3000], 'panel': [2054, 2201, 2219, 3002], 'probed': [2057], 'hVPS28-specific': [2059], 'primers': [2060, 2126, 2174, 3007], 'good': [2067], 'PCR': [2068, 2073, 2084, 2104, 2229, 2241, 2250, 3006, 3026, 3059], 'practices,': [2069], 'utilizing': [2071], 'touchdown': [2072], 'increase': [2075], 'specificity': [2077, 2536], 'reaction.': [2080], 'levels': [2082, 2210], 'also': [2087, 2845, 3124, 4264], 'analyzed': [2088, 2232, 3750], 'reactions': [2091, 2579, 3060], 'reached': [2092, 2236], 'plateau': [2094], '(i.e.before': [2095], 'saturation),': [2096], 'taking': [2100], 'samples': [2101, 2420, 2673, 2748, 2776], 'each': [2103, 2119, 3241, 3648, 4188, 4237], 'product': [2105, 3027, 3934], 'after': [2106, 2137, 3867], 'varying': [2107], 'times.': [2109], 'This': [2110, 4272], 'ensured': [2111], 'relative': [2114, 2223], 'abundance': [2115, 2224], 'target': [2117], 'compared.': [2124], 'amplify': [2129], 'located:': [2134], '(i)': [2135, 2176], 'just': [2136], 'initiation': [2139], 'codon,': [2140], '(ii)': [2142, 2188], '3′-untranslated': [2145, 2151, 3014], 'region.': [2146], 'primer': [2148], 'region': [2152, 3012, 3015, 3955, 3973], 'reduce': [2156], 'any': [2157], 'possible': [2158, 3196], 'risk': [2159], 'amplifying': [2161], 'member': [2164], 'same': [2167, 3596, 4334], 'family.': [2169], 'sequences': [2171], 'cat': [2177], 'ggg': [2178], 'atc': [2179], 'cca': [2180], 'gcc': [2181], 'acg': [2182], 'ccg': [2183], 'ggc': [2184, 2193, 2196], 'ata': [2185], 'gg': [2186], 'gtg': [2189], 'gat': [2190], 'gac': [2191], 'cac': [2192], 'ctg': [2194], 'tgt': [2195], 'gg.': [2197], 'normalized': [2205], 'mRNA': [2208, 3021], 'housekeeping': [2213], 'genes,': [2214], 'allowing': [2215], 'use': [2216], 'estimate': [2221], 'expression.': [2227], 'reaction': [2235], 'plateau,': [2238], 'monitoring': [2240], 'five': [2244], 'time': [2245], 'points': [2246], 'during': [2247, 3204, 3872], 'thermal': [2248], 'cycling.': [2249], 'run': [2253, 3713], 'parallel': [2255, 2419], 'size': [2258, 3050, 3746], '(MBI': [2260], 'Fermentas).': [2261], 'All': [2262], 'grown': [2265, 2313], "Dulbecco's": [2267], 'minimal': [2269], 'essential': [2270], "(Dulbecco's": [2272], "Eagle's": [2274], 'medium;': [2275], 'Life': [2276], 'Technologies,': [2277], 'Inc.)': [2278, 2656], '10%': [2280], 'fetal': [2281, 2354], 'calf': [2282, 2355], 'serum.': [2283], 'fluorescence': [2285], 'studies,': [2286], 'transfected': [2289, 2358], '∼30%': [2291], 'confluent': [2292], 'lipid-based': [2295], 'FuGene': [2296, 2308], '(Roche': [2298], 'Biochemicals).': [2300], 'Controls': [2301], 'without': [2305, 3498, 3564], 'used.': [2311, 3096], 'Cells': [2312, 2334], 'glass': [2315], 'coverslips': [2316], 'fixed': [2318], '18–24': [2319], 'h': [2320, 2766], 'post-transfection': [2321], 'methanol': [2323, 3205], '−20': [2325], '°C': [2326], 'mounted': [2328], 'ProLong': [2330], 'mount': [2331], '(Molecular': [2332], 'Probes).': [2333], 'Leica': [2339], 'confocal': [2340], 'microscope.': [2341], 'large': [2343], 'scale': [2344], 'transfections,': [2345], 'COS': [2346, 3741], 'passaged': [2349], 'HyClone': [2353], 'serum': [2356], 'ProFection': [2361], 'CaPO4-based': [2362], 'reagent': [2363], '(Promega).': [2364], 'COS-1': [2365], 'washed': [2368], 'homogenization': [2370], 'buffer': [2371, 2623, 2722], '(10': [2372], 'mm': [2373, 2380, 2461, 2466, 2469, 2533, 2625, 2630, 2724, 2729], 'triethanolamine,': [2374], 'pH': [2375, 2463, 2627, 2726], '7.4,': [2376, 2464, 2628], '1': [2377, 2468, 2517, 2532, 2637, 2865, 3017, 3101, 3122, 3130, 3160], 'mmMgCl2,': [2378], '250': [2379], 'sucrose)': [2381], 'harvested': [2383], 'scraping.': [2385], 'suspended': [2387], 'lysed': [2390], 'passaging': [2392], '20': [2393, 2460], 'times': [2394], 'fine': [2397], 'micropipette': [2398], 'tip,': [2399], 'postnuclear': [2401], 'supernatants': [2402, 2757], 'prepared': [2404, 2431, 2554], 'centrifugation': [2406], '5': [2408, 2686, 2735], 'min': [2409, 2442, 2736], '1000': [2411], '×': [2412, 2438], 'g.': [2413], 'Where': [2414], 'appropriate,': [2415], 'concentrations': [2417], 'equalized': [2422], 'dilution.': [2424], 'gel': [2426], 'filtration': [2427], 'experiments,': [2428, 2672], 'cytosols': [2429], 'centrifuging': [2433], 'extracts': [2435, 3881], '400,000': [2437], 'g': [2439], 'TL100': [2445], 'bench-top': [2446], 'ultracentrifuge': [2447], '(Beckman).': [2448], 'Samples': [2449, 3493], 'applied': [2451], '2.4-ml': [2454], 'Superose': [2455], '12': [2456], 'column': [2457], 'equilibrated': [2458], 'Hepes,': [2462], 'KOAc,': [2467], 'MgCl2,': [2470], 'fractionated': [2472, 3744], 'Amersham': [2475], 'Biotech': [2477], 'Smart': [2478], 'precipitated': [2482, 3337], 'chloroform/methanol': [2484], '(40Wessel': [2485], 'Flügge': [2487], 'U.I.': [2488], 'Anal.': [2489], 'Biochem...': [2490], '1984;': [2491], '138:': [2492], '141-143Google': [2493], 'processed': [2496], 'SDS-PAGE': [2498, 2542], 'immunoblotting.': [2500], 'vitrotranslations': [2502], 'procedures': [2507], 'reticulocyte': [2509, 3409], 'lysates': [2510], 'Promega.': [2512], 'Each': [2513], 'translation': [2514, 2538, 2590, 2614, 2676], 'typically': [2515, 3152], 'contained': [2516, 3446], 'μg': [2518], '60': [2522], 'μCi': [2523], '[35S]methionine.': [2525], 'Translations': [2526], 'terminated': [2528], 'addition': [2530, 2741, 4134], 'cycloheximide.': [2534], 'phosphorimaging.': [2544], 'generation': [2546], 'TDBA-lysyl': [2548, 2675], '[35S]protein,': [2549], 'TDBA-modified': [2550], 'lysyl': [2551, 3441], 'tRNA': [2552, 3442], 'described': [2556, 3937], '(41Görlich': [2558, 3452], 'Kurzchalia': [2560, 3454], 'T.V.': [2561, 3455], 'Wiedmann': [2562, 3456], 'Rapoport': [2564, 3458], 'T.A.': [2565, 3459], '1991;': [2569, 3463, 4064], '34:': [2570, 3464], '241-262Google': [2571, 3465], 'supplement': [2576], 'vitrotranslation': [2578], 'level': [2582, 2803], '40': [2584], 'pmol': [2585], 'per': [2586], 'μl': [2588, 2613, 2620, 2638, 2649, 2719, 2744], 'mix': [2591, 2615], '(42Swanton': [2592], 'E.': [2593], 'Sheehan': [2594], 'Bishop': [2596], 'High': [2598], '1633-1647Google': [2607], 'native': [2610, 2781, 3504], 'immunoprecipitations,': [2611, 2708], '5–20': [2612], 'diluted': [2617, 2679], '200': [2619], 'immunoprecipitation': [2622, 2746, 3355, 3385, 3582], '(20': [2624], 'Hepes-NaOH,': [2626], 'NaCl,': [2631, 2730], '1%': [2632, 2731], '(w/v)': [2633, 2732], 'Triton': [2634], 'X-100)': [2635], 'ascites': [2640], 'incubated': [2642, 2716, 2759, 3342, 3420, 3623, 3882], 'ice': [2644, 2752], '3–4': [2646], 'h.': [2647], 'G-agarose': [2652, 2710, 2771], 'beads': [2653, 2711, 2772], '(Zymed': [2654], 'Laboratories': [2655], 'added,': [2658, 2774], 'incubation': [2661, 3348, 3370], 'continued': [2662], 'overnight': [2663], 'constant': [2665], 'mixing,': [2666], 'prior': [2667, 3347], 'washing.': [2669], '5-fold': [2682], 'irradiated': [2684], 's': [2687], 'UV': [2690, 3499, 3518, 3551, 3565], 'lamp': [2691], '(SpotCure,': [2692], 'Ultra': [2693], 'Violet': [2694], 'Products': [2695], 'Ltd.,': [2696], 'UK).': [2698], 'Native': [2699], 'immunoprecipitations': [2700], 'normal.': [2704], 'denaturing': [2707, 2721, 3587], 'antigen': [2714], '30': [2718], '(100': [2723], 'Tris-HCl,': [2725], '7.6,': [2727], '140': [2728], 'SDS)': [2733], '95': [2738], '°C.': [2739, 2769], 'After': [2740, 4325], '170': [2743], 'buffer,': [2747], 'mixed': [2750], 'centrifuged.': [2754], 'resulting': [2756], 'second': [2762], 'treated': [2778, 3495], 'immunoprecipitations.': [2782], 'nucleotide': [2784], 'EMBLEST': [2791], 'W47604': [2794], 'determined.': [2796], 'comparisons': [2798], 'those': [2805, 3272], 'bases': [2809], '(GenBank™': [2821], 'U50630),': [2824], 'designated': [2827], '(Fig.1': [2829], 'A).': [2830, 3796], 'Comparison': [2831], 'base': [2838], 'Drosophila': [2848], 'melanogaster': [2849, 4268], '(SPTREMBL:': [2850, 2853, 2857, 2862], 'Q9V359),Caenorhabditis': [2851], 'elegans': [2852], 'Q9NA26),': [2854], 'Arabidopsis': [2855], 'thaliana': [2856, 4165], 'Q9S9T7),': [2858], 'Schizosaccharomyces': [2860], 'pombe': [2861, 4178], 'O13872)': [2863], '(Fig.': [2864, 3016, 3121, 3129, 3159, 3527, 3553, 3567, 3610, 3628, 3808, 3842, 3887, 4168, 4221], 'B).': [2866, 3810, 3844, 4223], 'Overall': [2867], 'comparison': [2868], 'six': [2875], 'species': [2876, 2948, 3759], 'revealed': [2877, 2986, 3138], 'overall': [2879, 2918, 4335], '34%': [2880], 'similarity.': [2882], 'Saccharomyces': [2883, 4153], 'cerevisiae': [2884], '(6Rieder': [2886], 'shows': [2903], '28%': [2904], 'identity': [2908], '38%': [2914], 'similarity,': [2915], 'giving': [2916], '66%.': [2922], 'Human': [2923], 'melanogaster.': [2933], 'major': [2935], 'difference': [2936], 'cerevisiaeVPS28': [2942], 'insertion': [2951], 'around': [2953, 3029], '20–35': [2954], 'acids': [2956], 'middle': [2959], 'hydrophilic': [2966], 'no': [2968, 3661, 3922], 'hydrophobic': [2969], 'regions': [2970], 'predicted': [2971, 4051, 4295], 'span': [2973], 'lipid': [2975], 'bilayer.': [2976], 'No': [2977, 3049, 3509, 3560], 'significant': [2978, 3975], 'similarities': [2979, 4186], 'known': [2981], 'searching': [2988], 'Prosite': [2990], 'pattern,': [2991], 'Blocks,': [2992], 'ProDom,': [2993], 'Prints': [2995], 'bases.': [2997], 'screened': [3004], '5′coding': [3011], 'C).': [3018], 'ubiquitous,': [3023], 'because': [3024, 3918], '750': [3030], 'bp': [3031], 'all': [3035, 3812], 'tissues': [3037], '(brain,': [3039], 'heart,': [3040], 'kidney,': [3041], 'liver,': [3042], 'lung,': [3043], 'pancreas,': [3044], 'placenta,': [3045], 'skeletal': [3047], 'muscle).': [3048], 'even': [3054], 'saturation': [3056], 'occurred': [3061], '(data': [3062, 3183, 3226, 3390, 3683], 'shown),': [3064], 'indicating': [3065], 'expressed.': [3072], 'determine': [3074], 'localization': [3077], 'VPS28,': [3080], 'fused': [3087], 'D,': [3102], 'NRK': [3106], 'exclusively': [3109], 'specifically': [3115], 'recognized': [3116, 3125], 'Western': [3119, 3752], 'blot': [3120], 'F)': [3123], 'E).': [3131], 'Staining': [3132], 'untransfected': [3133], 'anti-VPS28': [3136], 'endogenous': [3140, 3172, 3188], 'likewise': [3143], 'distributed': [3144], 'throughout': [3145], 'cytoplasm,': [3147], 'though': [3148], 'staining': [3150, 3174, 3213], 'more': [3153], 'granular': [3154], 'than': [3155], 'G).': [3161], 'present,': [3163], 'do': [3165], 'know': [3167], 'why': [3168], 'pattern': [3170], 'differs': [3175], 'Fractionation': [3181], 'shown)': [3185], 'indicate': [3186], 'largely': [3191], 'soluble.': [3192], 'much': [3198], 'pool': [3201], 'lost': [3203], 'fixation': [3206], 'immunofluorescence': [3208], 'staining.': [3209], 'particulate': [3211], 'did': [3214], 'co-localize': [3216], 'endosomes,': [3221, 3223], 'late': [3222], 'lysosomes': [3225], 'shown).': [3228, 3392, 3685], 'Evidence': [3229], 'suggests': [3232], 'many': [3234], 'complexes.': [3246], 'examine': [3248], 'whether': [3249, 3848, 3860], 'binds': [3251], '35S-labeled': [3259, 3417], 'vitro-translated': [3263], 'combined': [3265, 3524, 3539, 3606], 'tested': [3273], 'ability': [3276], 'bind': [3278, 3645], '2': [3330, 3528, 3532, 3554, 3568, 3611, 3629, 3634], 'A,': [3331], 'anti-myc': [3339, 3584, 3681], '(lane': [3344], '6).': [3345], 'hVPS28-His6': [3350, 3357, 3372, 3387, 3604, 3621], 'TSG101-myc/His6': [3352], 'allowed': [3353, 3383], 'efficient': [3354, 3384], 'anti-myc(lane': [3359], '7),': [3360], 'providing': [3361], 'interaction': [3365, 3397], 'Likewise,': [3369, 3660], 'HA-tag': [3382], 'anti-HA': [3389], 'mediated': [3403], 'additional': [3405, 3912], 'lysate,': [3410], 'approach': [3413], 'employed.': [3415], 'TSG101-His6': [3418, 3520, 3523, 3537], 'alone,': [3421], 'unlabeledmyc-tagged': [3424], 'translated': [3433], '4-(trifluoromethyldiazirino)benzoyl-N-hydroxysuccinic': [3438], '(TDBA)-modified': [3440], 'therefore': [3445], 'monovalent': [3448], 'UV-activated': [3449], 'moiety': [3451], '43Krieg': [3467], 'U.C.': [3468], 'Walter': [3469], 'Johnson': [3471], 'A.E.': [3472], 'Proc.': [3473, 4074], 'Natl.': [3474, 4075], 'Acad.': [3475, 4076], 'Sci.': [3476, 4077], 'U.': [3477, 4078], 'A...': [3479, 4080], '83:': [3481], '8604-8608Google': [3482], '44Brunner': [3484], 'Trends': [3486, 4061], '6:': [3490], '154-157Google': [3491], 'irradiation': [3500, 3552, 3566], 'immunoprecipitated': [3502, 3679], 'under': [3503, 3586], 'conditions': [3505, 3588], 'anti-His': [3507], 'antibody.': [3508], 'high': [3510, 3543, 3597, 3737], 'weight': [3512, 3545, 3599], 'adducts': [3514, 3547, 3561, 3575, 3600, 3698], 'observed': [3516, 3602], 'irradiating35S-labeled': [3519], 'or35S-labeled': [3522], 'TDBA-TSG101-myc': [3526, 3609], 'B,': [3529, 3590, 3612, 3630], 'top': [3530, 3591], 'panel,lanes': [3531], '4).': [3534, 3636], 'when35S-labeled': [3536, 3603, 3620, 3671], 'TDBA-hVPS28-myc,': [3541], 'radiolabeled': [3546], 'B,top': [3555, 3569], 'panel,': [3556, 3570, 3592, 3614, 3632], 'lane': [3557, 3571, 3593, 3615], '6,': [3558, 3616], 'asterisks).': [3559], '5).': [3572], 'Furthermore,': [3573], 'hVPS28-myc': [3580, 3800, 3816, 3828], '(Fig.2': [3589], '12,asterisks).': [3594], 'unlabeled': [3608], 'bottom': [3613, 3631], 'asterisks)': [3617], 'but': [3618, 3650], 'TDBA-myc-hVPS28': [3627], 'lanes': [3633], 'Together,': [3637], 'results': [3639], 'confirm': [3640], 'suggest': [3651], 'homodimers': [3656], 'formed.': [3659], 'homodimerization': [3664], 'His6-': [3672], 'myc-tagged': [3674], 'co-translated': [3677], 'apparent': [3687, 3762, 3866], 'masses': [3689], '∼80': [3691], '110': [3693], 'kDa': [3694, 3841], 'TSG101·hVPS28': [3697], 'TSG1011-hVPS281': [3705], 'TSG1011-hVPS282': [3707], 'species.': [3708], 'may': [3712], 'aberrantly': [3714], 'SDS-PAGE,': [3716], 'so': [3717], 'interpretations': [3720], 'possible.': [3725], 'TSG101/mVPS23': [3732], 'interact,': [3735], 'speed': [3738], 'supernatant': [3739], 'exclusion': [3747], 'chromatography': [3748], 'blotting.': [3753], 'migrated': [3755, 3817], 'single': [3758], 'mass': [3764, 3836], '350–400': [3766], 'kDa,': [3767], 'previous': [3770], '(15Babst': [3772, 4098], 'migration': [3787], 'precisely': [3791], 'coincident': [3792], '(Fig.3': [3795], 'overexpression': [3798], 'affected': [3801], 'stability': [3803, 3863], '3': [3809, 3843, 3888], 'detectable': [3815], 'position': [3820], 'monomer,': [3824], 'resulted': [3829], 'spread': [3832], '∼100': [3838], '400': [3840], 'unclear': [3847], 'complexes': [3850], 'destabilized': [3852], 'excess': [3857], 'hVPS28-myc,': [3858], 'reduced': [3862], 'only': [3865], 'dilution': [3868], 'chromatography.': [3873], 'Finally,': [3874], 'substantially': [3877], 'depleted': [3878], 'immobilized': [3884], 'C),': [3889], 'confirming': [3890], 'most,': [3892], 'if': [3893], 'all,': [3895], 'complexed': [3899], 'likely': [3904], '350-': [3907], '400-kDa': [3909], 'either': [3925], 'homodimerize.': [3930], 'mammaliantsg101': [3932], 'having': [3939], 'coiled': [3941, 4004], 'coil': [3942, 4005], 'encompassing': [3944], 'leucine': [3946, 4345], 'zipper': [3947], 'four': [3949], 'helical': [3950, 4350, 4375], 'turns': [3951], 'proline-rich': [3954, 4342], 'Subsequent': [3965], 'analysis': [3966, 4213], 'noted': [3967], 'homology': [3976], 'ubiquitin-conjugating': [3978, 4280], 'enzymes': [3979], 'nuclear': [4009], 'stathmin': [4058], '(58Sobel': [4059], '301-305Google': [4066], '59Maucuer': [4068], 'Camonis': [4070], 'J.H.': [4071], 'Sobel': [4072], '92:': [4082], '3100-3103Google': [4083], 'Recently,': [4085], 'geneSTP22': [4088], 'orthologous': [4093], 'mousetsg101': [4097], 'tsg101': [4113], 'highly': [4115], 'conserved.': [4116], 'Our': [4117], 'features': [4129], 'Homo': [4138], 'sapiens,': [4139], 'Mus': [4140], 'musculus,': [4141], 'cerevisiae,': [4144], 'potential': [4148, 4369], 'elegans,': [4152], 'carlbergensis': [4154], 'pastorianus,S.': [4155], 'pombe,': [4156], 'Chelonia': [4157], 'mydas': [4158, 4250], 'caranigra': [4159, 4251], '(Green': [4160], 'sea': [4161], 'turtle)': [4162], '(Mouse-ear': [4166], 'cress)': [4167], 'A),': [4170], 'start': [4173], 'conclusively': [4183], 'identified.': [4184], 'calculated': [4193], 'phylogenetic': [4212], 'create': [4217], 'unrooted': [4219], 'tree': [4220, 4225], 'indicates': [4226], '(95%': [4239], 'identical;': [4240, 4254], '99%': [4241], 'similar)': [4242], 'similar': [4247], '(89%': [4253], '97%': [4255], 'similar).': [4256], '(Accession': [4269], 'Q9VVA7).': [4271], 'lacks': [4274], '(': [4281], 'UbC)': [4282], '4C).': [4286], 'UbC': [4291, 4304, 4328], 'missing': [4293], 'delete': [4297], 'motif': [4299], 'inactive': [4302], 'E2': [4303], 'loop': [4310], 'active': [4313], 'UbCs': [4314], '(21Koonin': [4315], 'truncated': [4327], 'region,': [4329, 4343], 'retains': [4332], 'zipper,': [4346], 'domain.': [4351], 'relationship': [4354], 'unknown.': [4361], 'amphipathic': [4373], 'alpha': [4374], 'D),': [4380], 'det': [4382]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2102891026', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 5}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 5}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 5}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 4}, {'year': 2012, 'cited_by_count': 2}], 'updated_date': '2025-01-09T11:27:46.852445', 'created_date': '2016-06-24'}
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