Heterologous expression, purification and characterization of nitrilase from Aspergillus nigerK10

Name: Work Video:
Duration: 3 min 30 s
Ondřej Kaplan; Karel Bezouška; Ondřej Plíhal; Rüdiger Ettrich; Natalia Kulik; Ondřej Vaněk; Daniel Kavan; Oldřích Benada; Anna Malandra; Ondřej Šveda; A Veselá; Anna Rinágelová; Kristýna Slámová; Maria Cantarella; Jürgen Felsberg; Jarmila Dušková; Jan Dohnálek; Michael Kotik; Vladimı́r Křen; Ludmila Martı́nková
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'one': [226], 'month': [227], 'at': [228], '4°C': [229], 'fractionated': [231], 'gel': [233], 'filtration,': [234], 'fractions': [236, 244], 'were': [237, 245], 'electron': [240, 274], 'microscopy.': [241], 'late': [243], 'further': [246, 376], 'analyzed': [247], 'analytical': [249], 'centrifugation': [250], 'dynamic': [252], 'light': [253], 'scattering,': [254], 'shown': [256, 327], 'consist': [258], 'rather': [261], 'homogeneous': [262, 384], 'protein': [263, 381, 385], 'species': [264, 386], 'composed': [265], '12-16': [267], 'subunits.': [268], 'This': [269], 'hypothesis': [270], 'consistent': [272], 'microscopy': [275], 'our': [277], 'modelling': [278], 'multimeric': [281], 'nitrilase,': [282], 'supports': [284], 'arrangement': [286], 'dimers': [288], 'helical': [290], 'segments': [291], 'as': [292], 'plausible': [294], 'solution.': [296], 'Conclusions': [297], 'K10': [303], 'highly': [305], 'homologous': [306], '(≥86%)': [307], 'proteins': [309], 'deduced': [310], 'sequencing': 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