Title: Reviews on Strategies of Enhance Heterologous Protein Soluble Expression in Escherichia Coli
Abstract: Echerichia coli expression system has become a first choice to express heterologous protein because of its numerous advantages,but sometimes heterologous protein can make incorrect folding and form insoluble inclusion bodies in Escherichia coli. So researchers use a variety of methods to enhance heterologous protein expression,including change the expression,such as use of enhancing fusion tags,change of Escherichia coli strains,co-expression of molecular chaperone and folding regulator. This review summarized the common strategies to enhance heterologous protein expression.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 1
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