Abstract: The Schwartz technique is a simple method for labeling McAb directly with99mTc and can be utilized to design a one-step radiopharmacelltical kit. In this study, it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2-mercaptoethanol. The mean labeling efficiency of99mTc-3H11 was more than 95%. The immunoreactivity of99mTc-3H11 was more than 80% by ELISA’s method. Competition resultsin vitro showed that99mTc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney. The tumor uptake was 8.98±2.42% i.d/g. The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection. And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that99mTc-3H11 was stablein vivo and well good located at the lesion sites.
Publication Year: 1995
Publication Date: 1995-09-01
Language: en
Type: article
Indexed In: ['crossref']
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