Title: Quantification of the ninhydrin color reaction as applied to paper chromatography
Abstract: 1. The conditions necessary for the quantification of the ninhydrin color reaction have been defined and applied to the paper chromatographic determination of amino acids. Reproducible, quantitative determination of micro amounts of amino acids was achieved. Satisfactory recovery of ninhydrin-reactive compounds added to urine, plasma, and liver samples indicates that substances ordinarily found in urine and tissues do not interfere with the quantification procedure. 2. In addition, it is indicated that a ninhydrin spray made slightly alkaline with sodium hydroxide should be used when one assays for, or wishes to detect, taurine on chromatograms developed with acidic solvents.
Publication Year: 1956
Publication Date: 1956-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 131
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