Purified NPC1 Protein

Name: Work Video:
Duration: 3 min 30 s
Rodney E. Infante; Lina Abi-Mosleh; Arun Radhakrishnan; Jarrod D. Dale; Michael S. Brown; Joseph L. Goldstein
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1097, 1110, 1131, 1187, 1228, 1355, 1365, 1463, 2674, 3122, 3318, 3409, 3874, 3940, 4007, 4049], '(NPC1)': [5, 217, 573, 3892], 'is': [6, 34, 158, 218, 246, 370, 433, 454, 482, 537, 815, 1094, 1471, 3213, 3286], 'required': [7, 160, 219, 372], 'for': [8, 43, 161, 220, 255, 373, 1225, 1258, 1504, 2037, 2050, 2202, 2239, 2278, 2345, 2394, 2420, 2583, 2698, 2801, 2822, 2904, 3514, 3635, 3702, 3738, 3788, 3805, 4293, 4303, 4311, 4316, 4329, 4411, 4416, 4424], 'the': [9, 92, 101, 139, 162, 174, 201, 221, 304, 313, 351, 374, 386, 413, 428, 462, 485, 488, 545, 548, 556, 617, 620, 700, 774, 855, 886, 905, 920, 932, 1046, 1109, 1129, 1138, 1143, 1177, 1182, 1207, 1211, 1218, 1235, 1241, 1249, 1295, 1375, 1461, 1469, 1479, 1489, 1494, 1505, 1509, 1650, 1947, 1978, 2008, 2030, 2061, 2067, 2123, 2144, 2153, 2163, 2195, 2226, 2245, 2286, 2288, 2368, 2400, 2480, 2487, 2497, 2625, 2654, 2668, 2703, 2736, 2740, 2771, 3044, 3120, 3145, 3157, 3165, 3198, 3204, 3210, 3234, 3242, 3271, 3277, 3283, 3302, 3334, 3443, 3512, 3705, 3756, 4013, 4022, 4093, 4104, 4107, 4417, 4422], 'transport': [10, 222, 447, 1093, 1251], 'of': [11, 32, 131, 136, 155, 166, 176, 191, 203, 223, 244, 343, 348, 367, 378, 388, 403, 415, 507, 547, 558, 596, 610, 658, 702, 769, 819, 841, 844, 857, 883, 885, 1108, 1142, 1184, 1197, 1238, 1243, 1252, 1260, 1297, 1482, 1488, 1493, 1508, 1654, 1946, 1970, 1977, 1981, 1988, 1998, 2010, 2063, 2075, 2088, 2104, 2136, 2143, 2146, 2152, 2186, 2198, 2210, 2213, 2229, 2265, 2285, 2298, 2311, 2356, 2359, 2371, 2411, 2426, 2489, 2504, 2532, 2614, 2631, 2650, 2708, 2735, 2746, 2755, 2798, 2877, 2934, 2981, 3039, 3068, 3116, 3150, 3156, 3183, 3189, 3201, 3206, 3233, 3258, 3264, 3274, 3279, 3296, 3310, 3316, 3331, 3338, 3356, 3405, 3420, 3437, 3466, 3489, 3504, 3526, 3572, 3580, 3593, 3603, 3659, 3766, 3771, 3820, 3849, 3855, 3866, 3923, 3933, 3946, 3953, 3962, 3973, 3986, 3997, 4041, 4055, 4062, 4096, 4103, 4287, 4318, 4384, 4390, 4447, 4460], 'lipoprotein-derived': [12, 224, 703], 'cholesterol': [13, 225, 432, 453, 481, 536, 560, 611, 704, 924, 1015, 1074, 1092, 1122, 1239, 1261, 1515], 'from': [14, 62, 226, 274, 435, 456, 484, 563, 567, 629, 688, 705, 914, 1102, 1349, 1374, 1516, 1538, 1544, 1552, 1557, 1564, 1580, 1955, 2070, 2506, 2710, 2805, 3152, 3321, 3575, 4012, 4071, 4082, 4336, 4382, 4439, 4457, 4468], 'lysosomes': [15, 227, 451, 615, 706, 820, 860, 1517], 'to': [16, 82, 108, 193, 228, 294, 320, 405, 439, 487, 541, 555, 695, 916, 1013, 1126, 1128, 1134, 1194, 1206, 1371, 1473, 1518, 1974, 2005, 2029, 2162, 2178, 2673, 2678, 2729, 3042, 3167, 3244, 3353, 3448, 3475, 3537, 3733, 3778, 3983, 4253, 4266, 4473], 'endoplasmic': [17, 229, 489, 636], 'reticulum.': [18, 230], '1278-amino': [20, 232], 'acid,': [21, 233, 3492], 'polytopic': [22, 234, 900], 'membrane': [23, 45, 235, 257, 437, 901, 2643, 3745, 3780, 4322, 4466], 'has': [25, 237, 895, 1010, 1043, 1081, 1098], 'not': [26, 142, 152, 159, 238, 354, 364, 371, 1082, 1147, 2167], 'been': [27, 239, 896, 1011, 1044, 1084, 1100, 1113, 3909], 'purified,': [28, 240], 'and': [29, 51, 66, 78, 181, 241, 263, 278, 290, 393, 533, 593, 612, 622, 633, 821, 830, 926, 1041, 1105, 1140, 1231, 1262, 1323, 1383, 1451, 1485, 1496, 1533, 1550, 1561, 1568, 1573, 1615, 1617, 1656, 1711, 1764, 1778, 1799, 1818, 1837, 1860, 1876, 1892, 1909, 1925, 1940, 2044, 2176, 2225, 2306, 2374, 2441, 2559, 2576, 2608, 2622, 2637, 2667, 2692, 2808, 2817, 2825, 2849, 2880, 2937, 2959, 2984, 3025, 3033, 3089, 3119, 3132, 3186, 3261, 3328, 3387, 3459, 3498, 3534, 3596, 3607, 3615, 3710, 3730, 3760, 3782, 3862, 3970, 4034, 4075, 4178, 4192, 4205, 4218, 4234, 4251, 4284, 4306, 4321, 4341, 4408, 4434, 4443, 4462, 4465], 'its': [30, 80, 125, 242, 292, 337, 831, 1384], 'mechanism': [31, 243, 429, 557, 1087, 1510], 'action': [33, 245, 546], 'unknown.': [35, 247, 1095], 'Unexpectedly,': [36, 248], 'we': [37, 249, 1180, 1352], 'encountered': [38, 250], 'NPC1': [39, 69, 77, 113, 157, 195, 251, 281, 289, 325, 369, 407, 691, 780, 1090, 1130, 1450, 1484, 1513, 1934, 3574, 3964, 3999, 4079], 'in': [40, 117, 169, 185, 197, 205, 252, 329, 381, 397, 409, 417, 441, 450, 565, 614, 699, 767, 773, 898, 923, 931, 1075, 1115, 1204, 1240, 1387, 1649, 1708, 1983, 1990, 2014, 2066, 2082, 2091, 2109, 2113, 2169, 2173, 2206, 2223, 2274, 2308, 2340, 2352, 2385, 2438, 2486, 2529, 2550, 2594, 2647, 2739, 2768, 2892, 2970, 3136, 3144, 3164, 3195, 3209, 3241, 3268, 3282, 3290, 3301, 3306, 3333, 3511, 3585, 3590, 3598, 3637, 3661, 3712, 3752, 3877, 3949, 3978, 4021, 4058, 4067, 4184, 4189, 4194, 4224], 'a': [41, 44, 88, 118, 186, 253, 256, 300, 330, 398, 625, 697, 777, 839, 892, 928, 1120, 1185, 1195, 1227, 1354, 1502, 1941, 2011, 2015, 2041, 2083, 2110, 2114, 2182, 2189, 2207, 2250, 2258, 2331, 2353, 2405, 2556, 2562, 2572, 2663, 2777, 2829, 2852, 2886, 2964, 2987, 3000, 3057, 3075, 3092, 3126, 3291, 3307, 3390, 3413, 3430, 3724, 3817, 3829, 3899, 3994, 4028, 4072, 4076, 4337, 4342], 'search': [42, 254], 'that': [47, 259, 459, 814, 852, 894, 907, 1188, 1356, 1453, 1460, 2261, 2795, 3100, 3178, 3253, 4421], 'binds': [48, 260, 1073, 1189, 1357, 1455, 1464], '25-hydroxycholesterol': [49, 261, 647, 1190], '(25-HC)': [50, 262], 'other': [52, 264, 899, 1263, 1555], 'oxysterols.': [53, 167, 265, 379], 'A': [54, 266, 553, 1742, 2535, 2616, 2752, 3600, 3639, 3663, 3715], '25-HC-binding': [55, 267, 3085], 'was': [57, 141, 269, 353, 1124, 1381, 1706, 1953, 1972, 2003, 2026, 2035, 2120, 2130, 2157, 2247, 2270, 2291, 2303, 2320, 2327, 2349, 2402, 2418, 2432, 2527, 2545, 2568, 2592, 2645, 2660, 2676, 2686, 2705, 2727, 2748, 2759, 2774, 2815, 2847, 2871, 2910, 2928, 2975, 3101, 3110, 3123, 3141, 3160, 3237, 3351, 3410, 3423, 3473, 3496, 3519, 3654, 3747, 3800, 3814, 3825, 3842, 3875, 3916, 3941, 3965, 3991, 4050, 4426], 'purified': [58, 270, 1101, 1353, 1382, 1447, 1712, 2235, 2372, 3317, 3963], 'more': [59, 271], 'than': [60, 272], '14,000-fold': [61, 273], 'rabbit': [63, 275, 1350, 1483, 1495, 2521, 2756, 3153], 'liver': [64, 276, 621, 2230, 2522, 2757, 4458], 'membranes': [65, 277, 818, 1348, 2758], 'identified': [67, 279, 1442], 'as': [68, 280, 1366, 1444, 1647, 1661, 1713, 2126, 2446, 2761, 3162, 3239, 3549, 3671, 3993, 4019, 4110, 4413], 'by': [70, 121, 282, 333, 430, 539, 838, 1088, 1439, 1511, 1645, 1937, 1959, 2058, 2099, 2122, 2132, 2272, 2293, 2322, 2329, 2443, 2600, 2750, 2961, 3425, 3532, 3542, 3749, 3967, 4100, 4327], 'mass': [71, 283, 1440, 3546], 'spectroscopy.': [72, 284], 'We': [73, 285, 1222, 1362, 1446, 1457, 1476], 'prepared': [74, 286, 1644, 1660, 2081, 2098, 2760], 'recombinant': [75, 287, 1106, 1448, 1462], 'human': [76, 112, 288, 324, 508, 659, 824, 1449, 1497, 1616, 1933, 4097, 4258, 4282, 4418], 'confirmed': [79, 291, 1452], 'ability': [81, 293], 'bind': [83, 295, 1014, 3179, 3254], 'oxysterols,': [84, 296, 1200], 'including': [85, 297, 903], 'those': [86, 298], 'with': [87, 299, 569, 690, 1347, 1359, 2295, 2334, 2408, 2423, 2434, 2477, 2537, 2555, 2571, 2618, 2681, 2840, 2864, 2873, 2885, 2921, 2930, 2941, 2977, 2986, 3011, 3035, 3064, 3074, 3103, 3112, 3125, 3396, 3401, 3412, 3434, 3442, 3501, 3656, 3664, 3723, 3837, 3845, 3880, 3911, 3918, 3943, 4037, 4052, 4078, 4092, 4106, 4211, 4248, 4280, 4299], 'hydroxyl': [89, 301, 1208, 1214], 'group': [90, 302, 1209], 'on': [91, 100, 303, 312, 817, 1210, 1217, 2007, 2040, 2159, 2257, 2514], '24,': [93, 305], '25,': [94, 306], 'or': [95, 104, 307, 316, 859, 1079, 1985, 2021, 2094, 2138, 2234, 2364, 2380, 2390, 2494, 2516, 3336, 3726, 3929, 3957, 4289], '27': [96, 308], 'positions.': [97, 309], 'Hydroxyl': [98, 310], 'groups': [99, 311], '7,': [102, 314], '19,': [103, 315], '20': [105, 317, 1390, 3354, 3821, 3863], 'positions': [106, 318], 'failed': [107, 319], 'confer': [109, 321], 'binding.': [110, 322], 'Recombinant': [111, 323, 1703], 'also': [114, 326, 1458], 'bound': [115, 327, 2881, 3121, 3408], '[3H]cholesterol': [116, 328, 2076, 2381], 'reaction': [119, 331, 1145, 2179, 2333, 2348, 3350], 'inhibited': [120, 332], 'Nonidet': [122, 334, 661, 1562, 1794, 1813, 1832, 1852, 1874, 1890, 1923, 3927, 4010, 4046], 'P-40': [123, 335, 662, 1563], 'above': [124, 336, 2726, 2763, 4023, 4415], 'critical': [126, 338, 639], 'micellar': [127, 339, 497, 640], 'concentration.': [128, 340, 3046], 'Low': [129, 341, 4001], 'concentrations': [130, 342, 2145, 4286], 'unlabeled': [132, 145, 344, 357, 2089, 2337, 2478, 2499, 3341], '25-HC': [133, 172, 345, 384, 1192, 1230, 1247, 1358, 2338, 4288], 'abolished': [134, 346], 'binding': [135, 154, 196, 347, 366, 408, 503, 652, 1144, 1491, 2326, 2723, 3208, 3281], '[3H]cholesterol,': [137, 349, 2139], 'but': [138, 350, 1137, 1466], 'converse': [140, 352], 'true,': [143, 355], 'i.e.': [144, 356], 'cholesterol,': [146, 358, 1465], 'even': [147, 359], 'at': [148, 360, 1747, 1789, 1809, 1828, 1848, 1870, 1886, 1902, 1919, 2000, 2046, 2053, 2077, 2181, 2242, 2279, 2397, 2517, 2579, 2602, 2689, 2694, 2718, 2810, 3346, 3481, 3587, 3643, 3699, 3741, 3775, 3784, 3808, 3816, 4000, 4186, 4230, 4387], 'high': [149, 361, 1360], 'concentrations,': [150, 362], 'did': [151, 363], 'abolish': [153, 365], '[3H]25-HC.': [156, 368], 'known': [163, 375, 3980], 'regulatory': [164, 178, 376, 390, 514, 531, 669, 910, 929, 1236], 'actions': [165, 377, 1237], 'Thus,': [168, 380, 1246], 'NPC1-deficient': [170, 382], 'fibroblasts': [171, 383, 4070, 4220, 4419], 'blocked': [173, 385], 'processing': [175, 387], 'sterol': [177, 389, 668, 909, 1490, 2106, 2119, 2148, 2156, 2500], 'element-binding': [179, 391, 515, 670, 911], 'proteins': [180, 392, 912, 2882, 2938, 3071, 3177, 3252], 'activated': [182, 394], 'acyl-CoA:cholesterol': [183, 395, 551, 1326], 'acyltransferase': [184, 396, 1327], 'normal': [187, 399, 4073], 'fashion.': [188, 400], 'availability': [190, 402], 'assays': [192, 404, 2482], 'measure': [194, 406], 'vitro': [198, 410], 'may': [199, 411], 'further': [200, 412], 'understanding': [202, 414], 'ways': [204, 416], 'which': [206, 418, 431, 845, 1089, 1254, 1380, 1512, 2033, 2140, 2702, 3494, 3812, 3907, 3979, 4324], 'oxysterols': [207, 419, 1233], 'regulate': [208, 420], 'intracellular': [209, 421], 'lipid': [210, 422], 'transport.': [211, 423], 'Despite': [424], 'intense': [425], 'scientific': [426], 'interest,': [427], 'transported': [434, 483], 'one': [436, 1118], 'compartment': [438], 'another': [440, 1213], 'animal': [442, 1244], 'cells': [443, 566, 1710, 3582, 3631, 3706, 3767, 4105, 4181, 4244, 4263, 4296, 4470], 'remains': [444], 'obscure.': [445], 'One': [446], 'pathway': [448, 934], 'begins': [449], 'where': [452, 527, 534], 'liberated': [455], 'plasma': [457], 'lipoproteins': [458, 1589], 'have': [460, 696, 1111], 'entered': [461], 'cell': [463, 4173], 'through': [464, 544, 2561, 2733], 'receptor-mediated': [465], 'endocytosis': [466], '(1Brown': [467], 'M.S.': [468, 942, 960, 1023, 1052, 1274, 1310, 1329, 1400, 1424, 1597, 1632, 1665, 1689, 1723, 2456, 3368, 3680], 'Goldstein': [469, 939, 961, 1024, 1053, 1275, 1307, 1332, 1401, 1421, 1602, 1668, 1690, 1724, 2457, 3369, 3677], 'J.L.': [470, 940, 962, 1025, 1054, 1276, 1308, 1333, 1402, 1422, 1603, 1628, 1669, 1691, 1725, 2458, 3370, 3678], 'Science.': [471, 805, 4371], '1986;': [472], '232:': [473], '34-47Crossref': [474], 'PubMed': [475, 731, 749, 760, 809, 876, 952, 973, 1001, 1036, 1065, 1171, 1288, 1318, 1343, 1412, 1434, 1638, 1683, 1698, 1736, 2469, 3382, 3566, 3692, 4130, 4165, 4375, 4402], 'Scopus': [476, 732, 761, 810, 877, 953, 974, 1002, 1037, 1066, 1172, 1289, 1319, 1639, 1699, 1737, 2470, 3383, 3567, 3693, 4131, 4166, 4376], '(4362)': [477], 'Google': [478, 734, 750, 763, 812, 879, 955, 976, 1004, 1039, 1068, 1174, 1291, 1321, 1344, 1413, 1435, 1641, 1684, 1701, 1739, 2472, 3385, 3569, 3695, 4133, 4168, 4378, 4403], 'Scholar).': [479, 604, 764, 880, 1005, 1069, 1175, 1345, 1436, 1702, 1740, 2473, 3570, 3696, 4169, 4404], 'This': [480, 1499, 1951, 2684, 3247, 3823], 'lysosome': [486], 'reticulum': [490, 637], '(ER)': [491], '6The': [492], 'abbreviations': [493], 'used': [494, 2728, 3992], 'are:ERendoplasmic': [495], 'reticulumALLNN-acetyl-Leu-Leu-norleucinalCMCcritical': [496], 'concentrationCMVcytomegalovirusDTTdithiothreitolFCSfetal': [498], 'calf': [499, 645, 1619, 3624], 'serum25-HC25-hydroxycholesterolLPDSlipoprotein-deficient': [500], 'serumMOBPmembrane-bound': [501], 'oxysterol': [502, 651], 'proteinOSBPoxysterol-binding': [504], 'proteinhTERTcatalytic': [505], 'subunit': [506, 657, 4095], 'telomeraseNP-40Nonidet': [509], 'P-40NPC1Niemann-Pick,': [510], 'C1photo-[3H]25-HC[3β-3H]7,7-azocholestan-3β,': [512], '25-diolSREBPsterol': [513], 'proteinβ-VLDLβ-migrating': [516], 'very': [517, 673, 1586], 'low': [518, 674, 684, 1587], 'density': [519, 525, 675, 685, 1588, 4423], 'lipoproteinMES4-morpholineethanesulfonic': [520], 'acidNi-NTAnickel-nitrilotriacetic': [521], 'acidCHOChinese': [522], 'hamster': [523, 682, 1704], 'ovaryLDLlow': [524], 'lipoprotein.': [526, 686], 'it': [528, 1042, 1293, 1324, 1373, 1454, 2034, 3495], 'performs': [529], 'vital': [530], 'functions,': [532], 'excess': [535], 'stored': [538, 3510], 'conversion': [540], 'cholesteryl': [542], 'esters': [543], 'ER': [549, 915, 1242], 'enzyme': [550, 922], 'acyltransferase.': [552], 'clue': [554], 'this': [559, 1008, 1071, 1364], 'movement': [561], 'comes': [562], 'observations': [564], 'patients': [568, 689, 771], 'Niemann-Pick': [570], 'disease': [574, 692, 4080], '(2Pentchev': [575], 'P.G.': [576, 708, 753, 802, 4368], 'Vanier': [577, 713], 'M.T.': [578, 714], 'Suzuki': [579], 'K.': [580, 796, 4362], 'Patterson': [581], 'M.C.': [582], 'Scriver': [583], 'C.R.': [584], 'Beaudet': [585], 'A.L.': [586], 'Sly': [587], 'W.S.': [588], 'Valle': [589], 'D.': [590, 2894], 'Metabolic': [592], 'Molecular': [594], 'Basis': [595], 'Inherited': [597], 'Disease.': [598], 'McGraw-Hill': [599], 'Inc.,': [600], 'New': [601], 'York1995:': [602], '2625-2639Google': [603], 'These': [605], 'individuals': [606], 'accumulate': [607], 'large': [608, 848], 'amounts': [609, 2135, 3981], 'sphingolipids': [613], 'throughout': [616], 'body,': [618], 'particularly': [619], 'brain.': [623], 'As': [624, 2056, 2766], 'result,': [626], 'they': [627, 1202], 'suffer': [628], 'severe': [630], 'neurological': [631], 'disorders': [632], 'hepatic': [634], 'failure.': [635], 'N-acetyl-Leu-Leu-norleucinal': [638, 2540], 'concentration': [641, 2062, 2185, 2659, 3155, 3232, 3819, 3961], 'cytomegalovirus': [642, 1948], 'dithiothreitol': [643, 1758], 'fetal': [644, 3623], 'serum': [646, 649, 1621, 3625, 3988], 'lipoprotein-deficient': [648, 1620], 'membrane-bound': [650, 1186], 'oxysterol-binding': [654, 1368, 1377], 'catalytic': [656, 4094], 'telomerase': [660, 4098], '[3β-3H]7,7-azocholestan-3β,': [666, 1528], '25-diol': [667, 1529], 'β-migrating': [672, 1585], 'lipoprotein': [676], '4-morpholineethanesulfonic': [677], 'acid': [678, 680, 1535], 'nickel-nitrilotriacetic': [679], 'Chinese': [681], 'ovary': [683], 'Cells': [687, 3720, 4405], 'were': [693, 1146, 1223, 1643, 1659, 2080, 2097, 2171, 2483, 2511, 2715, 2796, 2803, 2883, 2899, 2939, 2953, 2997, 3031, 3054, 3072, 3087, 3299, 3446, 3454, 3509, 3530, 3583, 3632, 3707, 3721, 3895, 4025, 4090, 4182, 4221, 4245, 4264, 4297, 4314, 4325, 4380, 4406, 4437, 4471], 'shown': [694, 1012, 1125], 'defect': [698, 766], 'exit': [701], '(3Pentchev': [707], 'Comly': [709], 'M.E.': [710], 'Kruth': [711], 'H.S.': [712], 'Wenger': [715], 'D.A.': [716, 800, 4366], 'Patel': [717], 'S.': [718, 726, 968, 1060, 1166, 1609, 4140], 'Brady': [719], 'R.O.': [720], 'Proc.': [721, 963, 1055, 1161, 1604], 'Natl.': [722, 964, 1056, 1162, 1605], 'Acad.': [723, 965, 1057, 1163, 1606], 'Sci.': [724, 966, 1058, 1164, 1607, 4398], 'U.': [725, 967, 1059, 1165, 1608], 'A.': [727, 792, 938, 958, 969, 1017, 1050, 1061, 1167, 1610, 1673, 1717, 2450, 4358], '1985;': [728, 1312], '82:': [729], '8247-8251Crossref': [730], '(325)': [733], 'Scholar,': [735, 751, 956, 977, 1414, 1685, 4134], '4Liscum': [736], 'L.': [737], 'Faust': [738, 1303, 1666], 'J.R.': [739, 1304, 1667], 'J.': [740, 794, 865, 985, 990, 1268, 1277, 1334, 1403, 1425, 1674, 1692, 3362, 3371, 3560, 3681, 4360, 4395, 4396], 'Biol.': [741, 866, 991, 1278, 1335, 1404, 1426, 1675, 3372, 3682], 'Chem.': [742, 867, 992, 1279, 1336, 1405, 1427, 1676, 3373, 3683], '1987;': [743], '262:': [744], '17002-17008Abstract': [745], 'Full': [746, 871, 873, 947, 949, 996, 998, 1031, 1033, 1283, 1285, 1315, 1340, 1409, 1431, 1680, 1731, 1733, 2464, 2466, 3377, 3379, 3687, 3689], 'Text': [747, 872, 874, 948, 950, 997, 999, 1032, 1034, 1284, 1286, 1316, 1341, 1410, 1432, 1681, 1732, 1734, 2465, 2467, 3378, 3380, 3688, 3690], 'PDF': [748, 875, 951, 1000, 1035, 1287, 1317, 1342, 1411, 1433, 1682, 1735, 2468, 3381, 3691], '5Pentchev': [752], 'Biochim.': [754], 'Biophys.': [755, 4124], 'Acta.': [756], '2004;': [757, 1028, 1168, 1280, 1728, 2461, 3374], '1685:': [758], '3-7Crossref': [759], '(72)': [762], 'most': [768], 'these': [770], 'lies': [772], 'gene': [775], 'encoding': [776], 'designated': [779, 1363, 4176], '(6Loftus': [781, 4347], 'S.K.': [782, 1599, 1630, 4348], 'Morris': [783, 4349], 'J.A.': [784, 4142, 4350], 'Carstea': [785, 4351], 'E.D.': [786, 4352], 'Gu': [787, 4353], 'J.Z.': [788, 4354], 'Cummings': [789, 4355], 'C.': [790, 2972, 3138, 3398, 4356], 'Brown': [791, 941, 959, 1022, 1051, 1273, 1309, 1399, 1423, 1596, 1631, 1688, 1722, 2455, 3367, 3679, 4357], 'Ellison': [793, 4359], 'Ohno': [795, 4361], 'Rosenfeld': [797, 4363], 'M.A.': [798, 4364], 'Tagle': [799, 4365], 'Pentchev': [801, 4367], 'Pavan': [803, 4369], 'W.J.': [804, 4370], '1997;': [806, 4372], '277:': [807, 4373], '232-235Crossref': [808, 4374], '(699)': [811, 4377], 'Scholar)': [813, 1614, 1642, 3386, 4379], 'found': [816, 1459, 3142], 'endosomes.': [822], 'version': [825], 'contains': [826, 3175, 3250], '1278': [827], 'amino': [828, 850], 'acids,': [829], 'sequence': [832, 882, 893, 1009, 1072], 'predicts': [833], '13': [834, 1543], 'membrane-spanning': [835], 'helices': [836, 888], 'separated': [837, 3531], 'variety': [840], 'loops,': [842], 'three': [843, 2595], 'are': [846, 1255, 4175], 'quite': [847], '(∼240': [849], 'acids)': [851], 'project': [853], 'into': [854, 2547, 2956, 3429, 3456, 3461], 'lumen': [856], 'endosomes': [858], '(7Davies': [861], 'J.P.': [862], 'Ioannou': [863], 'Y.A.': [864], '2000;': [868], '275:': [869], '24367-24374Abstract': [870], '(234)': [878], 'five': [884], 'transmembrane': [887], '(helices': [889], '3-7)': [890], 'resembles': [891], 'observed': [897], 'proteins,': [902, 3781], 'Scap,': [904, 1007], 'transports': [908, 1514], '(SREBPs)': [913], 'Golgi;': [917], '3-hydroxy-3-methylglutaryl-CoA': [918, 1077], 'reductase,': [919, 1078], 'rate-controlling': [921], 'synthesis,': [925], 'Patched,': [927], 'Hedgehog': [933], '(8Hua': [935], 'X.': [936], 'Nohturfft': [937], 'Cell.': [943, 1027, 1311, 1727, 2460], '1996;': [944], '87:': [945], '415-426Abstract': [946], '(427)': [954], '9Nohturfft': [957], '1998;': [970, 1062], '95:': [971, 1063], '12848-12853Crossref': [972, 1064], '(111)': [975, 1067], '10Millard': [978], 'E.E.': [979], 'Gale': [980], 'S.E.': [981, 1331], 'Dudley': [982], 'N.': [983, 1150], 'Zhang': [984], 'Schaffer': [986], 'J.E.': [987], 'Ory': [988], 'D.S.': [989], '2005;': [993, 3563], '280:': [994], '28581-28590Abstract': [995], '(84)': [1003], 'In': [1006, 1117, 1176, 2188], '(11Radhakrishnan': [1016, 1716, 2449], 'Sun': [1018, 1718, 2451], 'L.-P.': [1019, 1719, 2452], 'Kwon': [1020, 1720, 2453, 3554], 'H.J.': [1021, 1721, 2454], 'Mol.': [1026, 1726, 2459], '15:': [1029, 1729, 2462], '259-268Abstract': [1030, 1730, 2463], '(261)': [1038, 1738, 2471], 'Scholar),': [1040, 1322], 'called': [1045, 1199], 'sterol-sensing': [1047], 'domain': [1048], '(9Nohturfft': [1049], 'Whether': [1070], 'NPC1,': [1076], 'Patched': [1080], 'yet': [1083], 'determined.': [1085], 'facilitates': [1091], 'never': [1099, 1112], 'native': [1103], 'membranes,': [1104], 'forms': [1107], 'studied': [1114], 'vitro.': [1116], 'study,': [1119], 'photoactivated': [1121], 'derivative': [1123], 'cross-link': [1127], 'when': [1132, 1468, 3203, 3276], 'added': [1133, 2028, 3474, 3815], 'intact': [1135], 'cells,': [1136], 'characteristics': [1139], 'specificity': [1141], 'determined': [1148, 2057, 2328, 3161, 3238], '(12Ohgami': [1149], 'Ko': [1151], 'D.C.': [1152], 'Thomas': [1153], 'M.': [1154], 'Scott': [1155], 'M.P.': [1156, 4148], 'Chang': [1157, 1159], 'C.C.Y.': [1158], 'T.-Y.': [1160], '101:': [1169], '12473-12478Crossref': [1170], '(169)': [1173], 'current': [1178], 'report,': [1179], 'describe': [1181, 1478], 'purification': [1183, 1481, 2741, 3200, 3273, 4459], '(25-HC).': [1191], 'belongs': [1193], 'class': [1196], 'sterols': [1198, 1556, 1989, 2090], 'because': [1201, 1229], 'contain,': [1203], 'addition': [1205], '3-position,': [1212], 'group,': [1215], 'usually': [1216], 'iso-octyl': [1219], 'side': [1220], 'chain.': [1221], 'searching': [1224], 'such': [1226], 'related': [1232], 'mimic': [1234], 'cells.': [1245], 'blocks': [1248], 'ER-to-Golgi': [1250], 'SREBPs,': [1253], 'transcriptional': [1256], 'activators': [1257], 'synthesis': [1259], 'lipids': [1264], '(13Adams': [1265, 3359], 'C.M.': [1266, 3360], 'Reitz': [1267, 3361], 'DeBrabander': [1269, 3363], 'J.K.': [1270, 3364], 'Feramisco': [1271, 3365], 'J.D.': [1272, 3366], '279:': [1281, 3375], '52772-52780Abstract': [1282, 3376], '(342)': [1290, 3384], 'Scholar);': [1292], 'accelerates': [1294], 'degradation': [1296], '3-hydroxy-3-methylglutaryl': [1298], 'CoA': [1299], 'reductase': [1300], '(14Gil': [1301], 'G.': [1302, 2428, 3839, 3913, 4150], 'Chin': [1305], 'D.J.': [1306], '41:': [1313], '249-258Abstract': [1314], '(258)': [1320], 'activates': [1325], '(15Brown': [1328], 'Dana': [1330], '1975;': [1337], '250:': [1338], '4025-4027Abstract': [1339], 'Working': [1346], 'liver,': [1351], 'affinity.': [1361], '“membrane-bound': [1367], 'protein”': [1369, 1378], '(MOBP)': [1370], 'distinguish': [1372], '“soluble': [1376], '(OSBP-1),': [1379], 'cDNA': [1385], 'cloned': [1386], 'our': [1388], 'laboratory': [1389], 'years': [1391], 'ago': [1392], '(16Dawson': [1393], 'P.A.': [1394, 1416], 'Ridgway': [1395], 'N.D.': [1396], 'Slaughter': [1397], 'C.A.': [1398], '1989;': [1406, 1428], '264:': [1407, 1429], '16798-16803Abstract': [1408], '17Dawson': [1415], 'van': [1417], 'der': [1418], 'Westhuyzen': [1419], 'D.R.': [1420], '9046-9052Abstract': [1430], 'Peptide': [1437], 'sequencing': [1438], 'spectroscopy': [1441], 'MOBP': [1443, 2505, 2709, 3151, 3181, 3202, 3256, 3275, 4461], 'NPC1.': [1445], '25-HC.': [1456, 3342], 'only': [1467], 'detergent': [1470, 2170, 2670], 'reduced': [1472], 'submicellar': [1474], 'concentrations.': [1475], 'here': [1477], 'initial': [1480], 'an': [1486, 1992, 2101, 2551, 3036, 3591, 3974], 'analysis': [1487, 1507, 3548], 'properties': [1492], 'proteins.': [1498], 'work': [1500], 'provides': [1501], 'beginning': [1503], 'eventual': [1506], 'ER.': [1519], 'Materials—We': [1520], 'obtained': [1521, 4438], '[26,27-3H]25-HC': [1522], '(75-80': [1523], 'Ci/mmol),': [1524, 1527], '[1,2,6,7-3H]cholesterol': [1525], '(60': [1526], '(20': [1530], 'Ci/mmol)': [1531], '(photo-[3H]25-HC),': [1532], '[1-14C]oleic': [1534], '(54.6': [1536], 'mCi/mmol)': [1537], 'American': [1539, 4083], 'Radiolabeled': [1540], 'Chemicals;': [1541], 'Fos-Choline': [1542, 1907, 3937], 'Anatrace;': [1545], 'anti-FLAG': [1546, 3891, 3903, 4030], 'M2-Agarose': [1547], 'affinity': [1548, 4032], 'beads': [1549, 2414, 3905], '6-methylcholesterol': [1551], 'Sigma;': [1553], 'all': [1554, 1574, 2192], 'Steraloids;': [1558], 'FuGENE': [1559, 3668], '6': [1560, 2424, 3515, 3644, 3669, 4038, 4231], 'Roche': [1565], 'Applied': [1566], 'Sciences;': [1567], 'Hybond': [1569], 'C': [1570, 1804, 2652, 2983, 3013, 3041, 3105, 3754, 4268], 'nitrocellulose': [1571], 'filters': [1572], 'chromatography': [1575], 'products': [1576], '(unless': [1577], 'otherwise': [1578], 'mentioned)': [1579], 'GE': [1581], 'Healthcare': [1582], 'Biosciences.': [1583], 'Rabbit': [1584, 2507, 2712], '(β-VLDL,': [1590], 'd': [1591, 1623], '<': [1592], '1.006)': [1593], '(18Kovanen': [1594], 'P.T.': [1595], 'Basu': [1598, 1629], 'Bilheimer': [1600], 'D.W.': [1601], '1981;': [1611], '78:': [1612], 'l396-l400Google': [1613], 'newborn': [1618], '(LPDS,': [1622], '>': [1624], '1.215': [1625], 'g/ml)': [1626], '(19Goldstein': [1627], 'Methods': [1633], 'Enzymol.': [1634], '1983;': [1635], '98:': [1636], '241-260Crossref': [1637], '(1284)': [1640], 'ultracentrifugation': [1646], 'described': [1648, 1663, 1715, 2448, 2725, 2762, 2767, 3163, 3240, 3551, 3672, 4020, 4112, 4414], 'indicated': [1651, 2227, 2369, 2498], 'reference.': [1652, 3995], 'Solutions': [1653], 'compactin': [1655], 'sodium': [1657, 1762, 1846, 4277], 'mevalonate': [1658], 'previously': [1662, 1714, 2447, 3550, 4111], '(20Brown': [1664], 'Kaneko': [1670], 'I.': [1671, 4064, 4118], 'Endo': [1672], '1978;': [1677], '253:': [1678], '1121-1128Abstract': [1679], '21Kita': [1686], 'T.': [1687], 'Clin.': [1693], 'Invest.': [1694], '1980;': [1695], '66:': [1696], '1094-1100Crossref': [1697], '(264)': [1700], 'His10-Scap(TM1-8)': [1705], 'produced': [1707], 'Sf9': [1709], 'Buffers—Buffer': [1741], 'contained': [1743, 1785, 1805, 1824, 1843, 1866, 1882, 1898, 1915, 2216, 2262, 3313], '50': [1744, 1750, 1786, 1806, 1825, 1867, 1883, 1899, 1916, 2217, 3482, 3870, 4272, 4275], 'mm': [1745, 1751, 1757, 1761, 1787, 1797, 1801, 1807, 1816, 1820, 1826, 1835, 1839, 1845, 1855, 1858, 1862, 1868, 1878, 1884, 1894, 1900, 1911, 1917, 1927, 2436, 2620, 2633, 2946, 3016, 3052, 3134, 3417, 3860, 3871, 3885], 'Tris-chloride': [1746, 1788, 1808, 1869, 1885, 1901, 1918], 'pH': [1748, 1790, 1810, 1829, 1849, 1871, 1887, 1903, 1920, 2787, 2861], '7.4,': [1749, 1791, 1811, 1850, 1872, 1888, 1904, 1921], 'KCl,': [1752, 1856], '10%': [1753, 1792, 4212, 4228], '(v/v)': [1754, 3622], 'glycerol,': [1755], '5': [1756, 4294], '(DTT),': [1759], '1': [1760, 1796, 1815, 1834, 1857, 2263, 2281, 2584, 3769, 4304], 'EDTA,': [1763, 1798, 1817, 1836, 1859], 'protease': [1765, 2623, 2638, 2655, 3757], 'inhibitor': [1766, 2656, 3758], 'mixture': [1767, 2246, 2401, 2685, 3602, 3759], '(1': [1768], 'μg/ml': [1769, 1773, 1776, 2539, 3617, 3718, 3762, 4207, 4301], 'pep-statin': [1770], 'A,': [1771], '2': [1772, 2240, 3464], 'aprotinin,': [1774], '10': [1775, 1844, 2335, 3339, 3846, 3852, 3920], 'leupeptin,': [1777], '200': [1779, 2435, 3884], 'μm': [1780, 2336, 3340, 4273, 4276], 'phenylmethysulfonyl': [1781], 'fluoride).': [1782], 'Buffer': [1783, 1803, 1822, 1841, 1864, 1880, 1896, 1913], 'B': [1784, 4196, 4226, 4255, 4270], '(w/v)': [1793, 1906, 2276], 'P-40,': [1795, 1814, 1833, 1853, 1875, 1891, 1924], '0.1': [1800, 1819, 1838, 1861, 3944, 4053], 'DTT.': [1802, 1821, 1840, 1863], '1%': [1812, 1831, 1851, 1873, 1889, 4009], 'D': [1823, 2821, 2842, 2866], 'MES-chloride': [1827], '5.5,': [1830], 'E': [1842, 2903, 2923, 2943], 'phosphate': [1847], '150': [1854, 1877, 1926], 'F': [1865], 'KCl.': [1879, 1895], 'G': [1881, 2020, 2093, 2440, 3804, 3857, 3868, 3882, 3925, 3956], '100': [1893, 1910, 2632, 3015, 3612, 3616, 4202, 4206], 'H': [1897, 2023, 2096, 3935], '0.1%': [1905], '13,': [1908], 'NaCl.': [1912], 'I': [1914, 2366, 4043], '0.004%': [1922, 4045], 'NaC1.': [1928], 'Plasmid': [1929], 'Construction—pCMV-NPC1-His8-FLAG': [1930], 'encodes': [1931], 'wild-type': [1932, 4338, 4433], 'followed': [1935, 2599, 2749, 3541], 'sequentially': [1936, 3844], 'eight': [1938], 'histidines': [1939], 'FLAG': [1942, 3947, 4056], 'tag': [1943], 'under': [1944], 'control': [1945], '(CMV)': [1949], 'promoter.': [1950], 'plasmid': [1952], 'constructed': [1954], 'pCMV-NPC1': [1956], '(Origene': [1957], 'Technologies)': [1958], 'site-directed': [1960], 'mutagenesis': [1961], '(QuikChange': [1962], 'II': [1963], 'XL': [1964], 'kit,': [1965], 'Stratagene).': [1966], 'coding': [1968], 'region': [1969], 'pCMV-NPC1-His8-FLAG': [1971, 3660], 'sequenced': [1973], 'ensure': [1975], 'integrity': [1976], 'construct.': [1979], 'Solubilization': [1980, 2503], 'Sterols': [1982, 2166], 'Detergent': [1984, 4002], 'Ethanol—For': [1986], 'solubilization': [1987], 'detergent,': [1991], 'ethanol': [1993, 2175, 2184, 2224, 2495], 'solution': [1994, 2103], 'containing': [1995, 2367, 2496, 2653, 3014, 3084, 3620, 3640, 3716, 3755, 3890, 3901, 4005, 4201, 4227, 4256, 4271], '4-5': [1996, 3657], 'nmol': [1997], '[3H]25-HC': [1999, 2065, 2137, 2203, 2219, 2290, 2319, 2377], '75-80': [2001], 'Ci/mmol': [2002, 2079], 'evaporated': [2004], 'dryness': [2006], 'sides': [2009], 'microcentrifuge': [2012, 2111], 'tube': [2013, 2112], 'SpeedVac': [2016, 2115], 'concentrator.': [2017, 2116], 'Detergent-containing': [2018], 'buffer': [2019, 2022, 2092, 2095, 2214, 2266, 2299, 2360, 2362, 2365, 2386, 2388, 2427, 2439, 2490, 2492, 2534, 2615, 2651, 2682, 2820, 2841, 2865, 2878, 2893, 2902, 2922, 2935, 2942, 2957, 2971, 2982, 3012, 3040, 3069, 3104, 3117, 3137, 3397, 3406, 3714, 3753, 3803, 3838, 3850, 3856, 3867, 3881, 3912, 3924, 3934, 3955, 3958, 4042, 4063], '(0.5': [2024], 'ml)': [2025], 'then': [2027, 2304, 2577, 2826, 3499, 3535, 3731, 3826, 3896, 4035, 4307], 'tube,': [2031], 'after': [2032, 2701, 3493, 3811], 'agitated': [2036], '4': [2038, 2243, 2398, 2518, 2690, 2719, 3113, 3347, 3742, 3776, 3809, 4427], 'h': [2039, 2241, 2396, 2824, 3516, 3807, 4305], 'vortex': [2042], 'mixer': [2043], 'centrifuged': [2045, 2578, 2693, 3783], '20,000': [2047], '×': [2048, 2581, 2589, 2604, 2696, 3645, 3735, 3786, 3794, 4232, 4236, 4428], 'g': [2049, 2582, 2590, 2697, 3736, 3787, 3795], '30': [2051, 2699, 3739, 3789], 'min': [2052, 2422, 3355, 3740], 'room': [2054], 'temperature.': [2055], 'scintillation': [2059, 2312, 2323, 2444, 3431, 3438, 3462, 3505], 'counting,': [2060], 'solubilized': [2064, 2121, 2168, 2753], 'supernatant': [2068, 2704, 3796], 'ranged': [2069], '0.4-1.5': [2071], 'μm.': [2072], 'Stock': [2073], 'solutions': [2074, 2087], '60': [2078], 'similar': [2084], 'manner.': [2085], 'Saturated': [2086], 'evaporating': [2100], 'ethanolic': [2102], 'each': [2105, 2598, 2734, 3349, 3421, 3476, 3484, 3652, 4312], '(50': [2107, 3982], 'μg)': [2108], 'dried': [2118, 2305], 'same': [2124, 2196], 'procedure': [2125], 'above.': [2127], 'recovery': [2129], 'estimated': [2131, 3966], 'adding': [2133], 'tracer': [2134, 2155], 'allowed': [2141], 'calculation': [2142], 'various': [2147, 3158, 3235, 4285], 'solutions.': [2149], 'choice': [2151], 'labeled': [2154], 'based': [2158], 'structural': [2160], 'similarity': [2161], 'bulk': [2164], 'sterol.': [2165], 'dissolved': [2172], '100%': [2174], 'delivered': [2177, 2222, 2339, 2384], 'mixtures': [2180], 'final': [2183, 2208, 2354, 2641, 2669, 3308, 3818], '1-4%.': [2187], 'given': [2190], 'experiment,': [2191], 'reactions': [2193], 'received': [2194, 3486], 'amount': [2197, 2228, 2370], 'ethanol.': [2199, 2341, 2391], 'Filter': [2200], 'Assay': [2201, 2344], 'Binding—Each': [2204, 2347], 'reaction,': [2205, 3305], 'volume': [2209, 2355, 3038, 3309], '120': [2211], 'μl': [2212, 2358, 3488], 'C,': [2215, 2958, 3070, 3118, 3407], 'nm': [2218, 2376, 3330], '(165-180': [2220, 2378], 'dpm/fmol)': [2221, 2379, 2383], 'extract,': [2231], 'column': [2232, 2406, 2417, 2781, 2833, 2857, 2870, 2875, 2927, 2932, 2968, 2974, 2979, 3006, 3066, 3098, 3109, 3114, 3325, 3394, 3403, 3835, 3841, 3847, 3853, 3864, 3900, 3915, 3921, 3931, 3951, 4018, 4033, 4039, 4060], 'fraction,': [2233], 'protein.': [2236], 'After': [2237, 2283, 2392, 3062, 3343, 3399, 3478, 3697, 4291], 'incubation': [2238, 2393, 3345, 3479, 3698, 4292], '°C,': [2244, 2399, 3348, 3483, 3810], 'loaded': [2248, 2403, 2775, 2827, 2850, 2911, 2998, 3090, 3388, 3827, 3897, 4026], 'onto': [2249, 2404, 2776, 2828, 2851, 2912, 2963, 2999, 3091, 3389, 3828, 3898, 4027], 'pretreated': [2251, 2271], 'glass-fiber': [2252], 'filter': [2253, 2269, 2302, 2722], '(G4,': [2254], 'Fisher)': [2255], 'placed': [2256, 3460], 'vacuum': [2259], 'apparatus': [2260], 'ml': [2264, 2297, 2310, 2410, 2425, 2531, 2613, 2630, 2649, 3027, 3436, 3465, 3503, 3770], 'F.': [2267, 2300, 4154], 'soaking': [2273], '0.3%': [2275], 'polyethyleneimine': [2277], 'least': [2280], 'h.': [2282, 2585, 2906], 'application': [2284], 'vacuum,': [2287], 'free': [2289], 'removed': [2292], 'washing': [2294, 3063, 3400], '2.5': [2296], 'immersed': [2307], '8': [2309, 2874, 2978, 3065, 3435, 3502, 4474], 'fluid': [2313, 3439], '(3a70B,': [2314], 'Research': [2315, 3470], 'Products': [2316, 3471], 'International).': [2317], 'Bound': [2318, 3873, 3939, 4048], 'measured': [2321, 2661], 'counting.': [2324], 'Nonspecific': [2325], 'incubating': [2330], 'duplicate': [2332], 'Ni-NTA': [2342, 2412], 'Agarose': [2343], '[3H]Sterol': [2346], 'carried': [2350, 2484, 2512, 2716], 'out': [2351, 2485, 2513, 2717], '80-140': [2357], 'G,': [2361, 2387, 2491, 3851], 'H,': [2363, 2389, 2493], 'NPC1-His8-FLAG': [2373], '10-400': [2375], '(130': [2382], '3': [2395, 2931, 3311], 'packed': [2407], '0.3': [2409], 'agarose': [2413], '(Qiagen).': [2415], 'Each': [2416, 2926, 3304], 'washed': [2419, 2593, 2642, 2872, 2929, 2976, 3111, 3843, 3917, 4036, 4246], '∼15': [2421], 'protein-bound': [2430], '[3H]sterol': [2431], 'eluted': [2433, 2884, 2940, 2949, 2985, 3073, 3082, 3124, 3411, 3876, 3888, 3942, 4006, 4051], 'imidazole': [2437, 3813], 'quantified': [2442, 3424], 'counting': [2445], 'For': [2474], 'competition': [2475], 'experiments': [2476, 3298, 3636, 4412], 'sterols,': [2479], 'standard': [2481], 'presence': [2488, 3337], '(0-10': [2501], 'μm).': [2502], 'Liver': [2508], 'Membranes—All': [2509], 'operations': [2510, 2714], 'ice': [2515], '°C.': [2519, 2720, 2812, 3743], 'Frozen': [2520], '(200-300': [2523], 'g,': [2524], 'Pel-Freez': [2525], 'Biologicals)': [2526], 'thawed': [2528, 2543, 2816], '300': [2530, 2612, 2629, 2648, 3003], 'cold': [2533], 'supplemented': [2536, 2617, 4210, 4279], '25': [2538, 3717, 3761, 3859, 3930, 4300], '(ALLN).': [2541], 'tissue': [2544, 3467], 'shredded': [2546], 'small': [2548], 'pieces': [2549], 'Oster': [2552], 'blender,': [2553], 'homogenized': [2554, 2570, 3722], 'Polytron': [2557], 'homogenizer,': [2558], 'filtered': [2560], 'triple-layered': [2563], 'cheese': [2564], 'cloth.': [2565], 'filtrate': [2567], 'again': [2569], '100-ml': [2573], 'Dounce': [2574, 3728, 3750], 'homogenizer': [2575, 3729], '100,000': [2580, 2588, 2603, 2695, 3734, 3785, 3793], 'resulting': [2587, 3792, 4171], 'pellet': [2591, 2644, 3746], 'sequential': [2596], 'steps,': [2597], 'centrifugation': [2601, 3737], 'g.': [2605], 'first': [2607], 'third': [2609], 'steps': [2610, 2738], 'employed': [2611, 2628], '450': [2619], 'KCl': [2621, 2888, 2989, 3017, 3077, 3128, 3147], 'inhibitors;': [2624], 'second': [2626], 'step': [2627, 3127], 'Na2CO3': [2634], '(pH': [2635, 3192, 3216], '11.3)': [2636], 'inhibitors.': [2639], 'resuspended': [2646, 3711, 3748], 'mixture.': [2657], 'Protein': [2658, 3231, 3960], 'using': [2662, 3667], 'BCA': [2664], 'kit': [2665], '(Pierce),': [2666], '(Nonidet': [2671], 'P-40)': [2672, 3928, 4011], 'ratio': [2675], 'adjusted': [2677], '4.5': [2679], '(w/w)': [2680], 'B.': [2683], 'rotated': [2687], 'overnight': [2688, 3344, 3480, 3774], '°C': [2691, 3589, 3701, 3777, 4188], 'min,': [2700], 'collected.': [2706], 'Purification': [2707, 3571], 'Detergent-solubilized': [2711], 'Liver—All': [2713], 'assay': [2724], 'follow': [2730], '[3H]25-HC-binding': [2731], 'activity': [2732, 2898, 2952, 2996, 3086, 3140], 'seven': [2737], '(Table': [2742, 2789, 3326], '1).': [2743, 2765], 'content': [2745], 'OSBP-1': [2747, 3187, 3207, 3262, 3280], 'immunoblotting.': [2751], 'extract': [2754, 2773, 4320, 4464], '(Step': [2764, 2843, 2867, 2924, 3018, 3106], 'Fig.': [2769, 3168, 3245], '1,': [2770, 2790], 'soluble': [2772], '20-ml': [2778, 2830], 'Q-Sepharose': [2779, 2854], 'ion-exchange': [2780, 2832, 2967, 3060, 3393], '(Hi': [2782, 2834], 'Prep': [2783, 2835], '16/10': [2784, 2836], 'Q': [2785, 2859], 'FF,': [2786], '7.4)': [2788], 'Step': [2791], '2).': [2792], 'Active': [2793], 'fractions': [2794, 2896, 2950, 2994, 3021, 3049, 3083, 3159, 3236, 3879, 3889, 4004, 4456, 4467], 'devoid': [2797], 'immunoblot': [2799, 3893], 'reactivity': [2800, 3894], 'OSBP': [2802], 'pooled': [2804, 3032, 3088, 4315], 'multiple': [2806], 'runs': [2807], 'frozen': [2809], '-80': [2811], 'Pooled': [2813], 'material': [2814, 2909, 3824], 'dialyzed': [2818, 2900, 2908, 2955, 3801], 'against': [2819, 2901, 3802], '6-12': [2823, 2905, 3806], 'SP-Sepharose': [2831], 'SP': [2837], 'FF)': [2838], 'pre-equilibrated': [2839, 2863, 2920, 2969, 3010, 3102, 3395, 3836, 3910], '3).': [2844], 'flow-through': [2846], 'collected': [2848], '5-ml': [2853], 'ion': [2855], 'exchange': [2856], '(HiTrap': [2858], 'HP,': [2860], '5.5)': [2862], '4).': [2868], 'volumes': [2876, 2933, 2980, 3067, 3115, 3404, 3848, 3854, 3865, 3922, 3932, 3952, 4040, 4061], 'D,': [2879], 'continuous': [2887, 2988, 3076], 'gradient': [2889, 2990, 3078, 3129, 3415], '(0-500': [2890, 2991, 3079, 3416], 'mm)': [2891], 'containing-binding': [2897, 2951, 2995], '5-10': [2913], '2-ml': [2914, 3093], 'RCA': [2915], 'Lectin': [2916, 3219], 'columns': [2917], '(EY': [2918], 'Laboratories)': [2919], '5).': [2925], 'E,': [2936], 'plus': [2944, 3858, 3869, 3883], '250': [2945], 'α-Lactose.': [2947], 'pooled,': [2954], 'concentrated': [2960, 3055], 'loading': [2962], '1-ml': [2965, 3058, 3391, 3830, 3902, 4029], 'Mono-Q': [2966, 3059, 3392], 'mm).': [2992, 3080], '320-ml': [3001], 'Sephacryl': [3002], 'gel': [3004, 3223, 3452, 3977], 'filtration': [3005, 3224], '(HiPrep': [3007], '26/60': [3008], 'S-300)': [3009], '6).': [3019], 'eluting': [3022, 3320], 'between': [3023], '112': [3024], '140': [3026], '(containing': [3028, 3050, 3797], 'peak-binding': [3029], 'activity)': [3030], 'mixed': [3034], 'equal': [3037], 'decrease': [3043], 'salt': [3045, 3414], 'diluted': [3048], '∼50': [3051], 'KCl)': [3053, 3135], 'over': [3056], 'column.': [3061], 'Reactive': [3094, 3322], 'Blue': [3095, 3228, 3323], 'Dye': [3096], '72': [3097, 3324], '(Sigma)': [3099], '7).': [3107], '(100,': [3130], '250,': [3131], '500': [3133, 3329], 'Binding': [3139], 'primarily': [3143], '250-mm': [3146], 'fraction.TABLE': [3148], '1Purification': [3149], 'liverStepFractionProteinaProtein': [3154], 'legend': [3166, 3243], '1.Specific': [3169], 'activityTotal': [3170], 'activityPurificationRecoverymgpmol/mgpmol-fold%1Solubilized': [3171], 'membranesbThis': [3172], 'starting': [3173, 3211, 3248, 3284], 'fraction': [3174, 3212, 3249, 3285, 3422], 'two': [3176, 3251], '[3H]25-HC:': [3180, 3255], '(∼25%': [3182, 3257], 'total': [3184, 3190, 3259, 3265], 'binding)': [3185, 3260], '(∼75%': [3188, 3263], 'binding).29,0080.013001002Q-Sepharose': [3191], '7.4)1,7270.1933219': [3193], '(74)cNumbers': [3194], 'parenthesis': [3196, 3269], 'denote': [3197, 3270], '-fold': [3199, 3272], 'contribution': [3205, 3278], 'subtracted.1103SP-Sepharose8460.3529534': [3214], '(135)984Q-Sepharose': [3215], '5.5)921.6144150': [3217], '(601)485RCA': [3218], '→': [3220, 3225], 'Mono-Q93.631347': [3221], '(1,388)106S-300': [3222], 'Mono-Q93.229309': [3226], '(1,237)107Reactive': [3227], '720.737.4253618': [3229], '(14,471)8a': [3230], '1.b': [3246], 'binding).c': [3266], 'Numbers': [3267], 'subtracted.': [3287], 'Open': [3288], 'table': [3289], 'new': [3292], 'tab': [3293], 'Photoaffinity': [3294], 'Labeling': [3295], 'MOBP—All': [3297], 'performed': [3300], 'dark.': [3303], 'ml,': [3312], '0.133': [3314], 'mg': [3315], '(fraction': [3319], '1)': [3327], 'photo-[3H]25-HC': [3332], 'absence': [3335], 'subjected': [3352, 3447, 3536, 3732, 4472], 'UV': [3357], 'irradiation': [3358], '12': [3402], 'KCl).': [3418], 'Radioactivity': [3419], 'applying': [3426], '20-μl': [3427], 'aliquots': [3428], 'vial': [3432], 'filled': [3433, 3500], '3a70b.': [3440], 'Samples': [3441], 'highest': [3444], 'radioactivity': [3445, 3518], '8%': [3449, 3975], 'SDS-PAGE.': [3450], 'Individual': [3451], 'lanes': [3453], 'cut': [3455], '3-mm': [3457], 'slices': [3458], 'vials.': [3463], 'solubilizer': [3468], '(TS-2,': [3469], 'International)': [3472], 'vial.': [3477], 'sample': [3485], '400': [3487, 3984], 'glacial': [3490], 'acetic': [3491], 'shaken': [3497], 'fluid.': [3506], 'vials': [3508], 'dark': [3513], 'before': [3517], 'measured.': [3520], 'Liquid': [3521], 'Chromatography/Tandem': [3522], 'Mass': [3523], 'Spectrometry': [3524], 'Analysis': [3525], 'Photo-[3H]25-HC-labeled': [3527], 'Proteins—Protein': [3528], 'samples': [3529], 'SDS-PAGE': [3533, 3976], 'in-gel': [3538], 'tryptic': [3539], 'digestion': [3540], 'high-performance': [3543], 'liquid': [3544], 'chromatography/tandem': [3545], 'spectrometry': [3547], '(22Chen': [3552], 'Y.': [3553, 3559], 'S.W.': [3555], 'Kim': [3556], 'S.C.': [3557], 'Zhao': [3558], 'Proteome': [3561], 'Res.': [3562, 4125], '4:': [3564], '998-1005Crossref': [3565], '(159)': [3568], 'Epitope-tagged': [3573, 3998], 'Transfected': [3576], 'CHO': [3577], 'Cells—Stock': [3578], 'cultures': [3579, 4335], 'CHO-K1': [3581, 3630], 'grown': [3584, 4183], 'monolayer': [3586, 4185], '37': [3588, 3700, 4187], 'atmosphere': [3592], '8-9%': [3594], 'CO2': [3595, 4191], 'maintained': [3597, 4193], 'medium': [3599, 3606, 3638, 3662, 4195, 4200, 4225, 4254, 4267], '(1:1': [3601], "Ham's": [3604], 'F-12': [3605], "Dulbecco's": [3608], 'modified': [3609, 4198], "Eagle's": [3610, 4199], 'medium,': [3611], 'units/ml': [3613, 4203], 'penicillin,': [3614], 'streptomycin': [3618, 4208], 'sulfate)': [3619, 4209], '5%': [3621, 3641, 3665, 4190, 4257, 4281], '(FCS).': [3626], 'On': [3627, 3649, 4214, 4241, 4260], 'day': [3628, 3650, 4215, 4242, 4261], '0,': [3629, 4216], 'set': [3633, 4222, 4409], 'up': [3634, 4223, 4410], 'FCS': [3642, 4229], '105': [3646], 'cells/100-mm': [3647], 'dish.': [3648, 4431], '2,': [3651, 4243], 'dish': [3653], 'transfected': [3655], 'μg': [3658], 'FCS,': [3666], 'reagent': [3670], '(23Rawson': [3673], 'R.B.': [3674], 'DeBose-Boyd': [3675], 'R.A.': [3676], '1999;': [3684, 4127], '274:': [3685], '28549-28556Abstract': [3686], '(149)': [3694], '24': [3703], 'h,': [3704, 4295], 'harvested,': [3708], 'washed,': [3709], 'ice-cold': [3713], 'ALLN.': [3719], '15-ml': [3725], '40-ml': [3727], 'homogenization': [3751], 'ALLN': [3763, 4302], '(4': [3764], 'dishes': [3765, 4310], 'per': [3768], 'buffer),': [3772], 'incubated': [3773], 'solubilize': [3779], 'min.': [3790], 'detergent-solubilized': [3798], 'membranes)': [3799], 'mm.': [3822], 'His': [3831, 4014], 'Trap': [3832, 4015], 'HP': [3833, 4016], 'nickel': [3834, 4017], 'imidazole,': [3861], 'imidazole.': [3872, 3886], '1.5-ml': [3878], 'M2-agarose': [3904, 4031], '(Sigma),': [3906], 'had': [3908], 'either': [3919, 3954], '(1%': [3926], '(0.1%': [3936], '13).': [3938], 'mg/ml': [3945, 4054], 'peptide': [3948, 4057], '9': [3950, 4059], 'H.': [3959], 'silver': [3968], 'staining': [3969], 'densitometric': [3971], 'scanning': [3972], 'ng)': [3985], 'bovine': [3987], 'albumin': [3989], '(Pierce)': [3990], 'Preparation': [3996], 'Concentration—The': [4003], '(in': [4008, 4044], 'section': [4024], 'P-40).': [4047], 'SREBP-2': [4065], 'Processing': [4066], 'Cultured': [4068], 'Cells—Skin': [4069], 'subject': [4074], 'patient': [4077], '(obtained': [4081], 'Culture': [4085], 'Collection,': [4086], 'ATTC': [4087], 'no.': [4088], 'GM3123)': [4089], 'immortalized': [4091, 4172], '(hTERT)': [4099], 'retroviral': [4101], 'infection': [4102], 'Babe-puro-hTERT': [4108], 'vector': [4109], '(24Ouellette': [4113], 'M.M.': [4114], 'Aisner': [4115], 'D.L.': [4116], 'Savre-Train': [4117], 'Wright': [4119, 4145], 'W.E.': [4120, 4146], 'Shay': [4121, 4155], 'J.W.': [4122, 4156], 'Biochem.': [4123], 'Commun.': [4126], '254:': [4128], '795-803Crossref': [4129], '(99)': [4132], '25Wood': [4135], 'L.D.': [4136], 'Halvorsen': [4137], 'T.L.': [4138], 'Dhar': [4139], 'Baur': [4141], 'Pandita': [4143, 4159], 'R.K.': [4144], 'Hande': [4147], 'Calaf': [4149], 'Hei': [4151], 'T.K.': [4152, 4160], 'Levine': [4153], 'Wang': [4157], 'J.J.Y.': [4158], 'Oncogene.': [4161], '2001;': [4162], '20:': [4163], '278-288Crossref': [4164], '(87)': [4167], 'lines': [4174], 'hTERT-Control': [4177, 4217], 'hTERT-NPC1.': [4179], "(Dulbecco's": [4197], 'penicillin': [4204], 'FCS.': [4213], 'hTERT-NPC1': [4219], '104': [4233, 4237, 4429], '7': [4235], 'cells/60-mm': [4238, 4430], 'dish,': [4239], 'respectively.': [4240], 'once': [4247], 'phosphate-buffered': [4249], 'saline': [4250], 'switched': [4252, 4265], 'LPDS.': [4259], '4,': [4262], '(medium': [4269], 'compactin,': [4274], 'mevalonate)': [4278], 'LPDS': [4283], 'β-VLDL.': [4290], 'treated': [4298], 'harvested.': [4308], 'Six': [4309], 'condition': [4313], 'preparation': [4317], 'nuclear': [4319, 4463], 'fractions,': [4323], 'analyzed': [4326], 'immunoblotting': [4328], 'SREBP': [4330], '(described': [4331], 'below).': [4332], 'Primary': [4333], 'fibroblast': [4334], 'BALB/c': [4339, 4344], 'mouse': [4340, 4346], 'mutant': [4343, 4435], 'npcnih': [4345], 'established': [4381], 'explants': [4383], 'skin': [4385], 'taken': [4386], '7-8': [4388], 'weeks': [4389], 'age': [4391], '(26Willnow': [4392], 'T.E.': [4393], 'Herz': [4394], 'Cell': [4397], '1994;': [4399], '107:': [4400], '719-726Crossref': [4401], 'cultured': [4407, 4469], 'except': [4420], 'plating': [4425], 'mice': [4436], 'Drs.': [4440], 'Stephen': [4441], 'Turley': [4442], 'John': [4444], 'Dietschy': [4445], '(University': [4446], 'Texas': [4448], 'Southwestern': [4449], 'Medical': [4450], 'Center,': [4451], 'Dallas,': [4452], 'TX).': [4453], 'Immunoblot': [4454], 'Analysis—Column': [4455]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2083957918', 'counts_by_year': [{'year': 2024, 'cited_by_count': 9}, {'year': 2023, 'cited_by_count': 8}, {'year': 2022, 'cited_by_count': 8}, {'year': 2021, 'cited_by_count': 6}, {'year': 2020, 'cited_by_count': 12}, {'year': 2019, 'cited_by_count': 6}, {'year': 2018, 'cited_by_count': 13}, {'year': 2017, 'cited_by_count': 8}, {'year': 2016, 'cited_by_count': 12}, {'year': 2015, 'cited_by_count': 4}, {'year': 2014, 'cited_by_count': 7}, {'year': 2013, 'cited_by_count': 15}, {'year': 2012, 'cited_by_count': 19}], 'updated_date': '2024-12-14T10:11:26.832684', 'created_date': '2016-06-24'}
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