Title: Anthralin (dithranol)<i>in vitro</i>inhibits human monocytes to secrete IL-6, IL-8 and TNF-α, but not IL-1
Abstract: British Journal of DermatologyVolume 136, Issue 4 p. 542-547 Anthralin (dithranol) in vitro inhibits human monocytes to secrete IL-6, IL-8 and TNF-α, but not IL-1 U. MROWIETZ, U. MROWIETZ Department of Dermatology. University of Kiel. Schittenhelmstr. 7. D-24105, Kiel, GermanySearch for more papers by this authorH. JESSAT, H. JESSAT Department of Dermatology. University of Kiel. Schittenhelmstr. 7. D-24105, Kiel, GermanySearch for more papers by this authorA. SCHWARZ, A. SCHWARZ Ludwig Bolzmann Institute for Cell Biology and Immunobiology of the Skin. Department of Dermatology. University of Münster. Münster. GermanySearch for more papers by this authorT. SCHWARZ, T. SCHWARZ Ludwig Bolzmann Institute for Cell Biology and Immunobiology of the Skin. Department of Dermatology. University of Münster. Münster. GermanySearch for more papers by this author U. MROWIETZ, U. MROWIETZ Department of Dermatology. University of Kiel. Schittenhelmstr. 7. D-24105, Kiel, GermanySearch for more papers by this authorH. JESSAT, H. JESSAT Department of Dermatology. University of Kiel. Schittenhelmstr. 7. D-24105, Kiel, GermanySearch for more papers by this authorA. SCHWARZ, A. SCHWARZ Ludwig Bolzmann Institute for Cell Biology and Immunobiology of the Skin. Department of Dermatology. University of Münster. Münster. GermanySearch for more papers by this authorT. SCHWARZ, T. SCHWARZ Ludwig Bolzmann Institute for Cell Biology and Immunobiology of the Skin. Department of Dermatology. University of Münster. Münster. GermanySearch for more papers by this author First published: 28 June 2008 https://doi.org/10.1046/j.1365-2133.1997.d01-1232.xCitations: 13AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Summary Anthralin is a most widely used compound for topical treatment of psoriasis. Whereas numerous studies have ascertained anthralin as a safe and effective drug its mode of action still remains unclear. Previous studies demonstrated dose-dependent inhibition of a number of pro-inflammatory functions in human enutrophils and monocytes (MO). The aim of the present study was to investigate in stimulated MO the effect of anthralin on the secretion of cytokines which are of known importance for the psoriatic tissue reaction. Highly purifies MO were incubated with anthralin (0·01–1·0μg/ml), its clinically inactive derivative danthrone (0·1 and 10 μg/ml), the solvent acetone, or medium alone. Culture supernatants were analysed for immunoreactivity for interleuking-1β, and -8 (IL-1β, IL-8), and tumour necrosis factor β (TNF-β) by specific ILISA. IL-6 bioactivity was determined using the B9-bioassay. Additionally, IL-1 bioactivity was measured by the D10[N4]M-bioassay. The results show a dose-dependent inhibition of MO IL-6, IL-8, and TNF-α release with a half- maximal inhibitory concentration of 0.25–0.6μ/ml of anthralin. There was no effect of danthrone or acetone on the secretion of these cytokines from MO. Secretion of IL-1β immunoreactivity measured by ELISA as determination of biological activity of IL-1 using the D10[N4]M- bioassay revealed a slight increase in IL-1 secretion with a maximum at an anthralin concentration of 0.1 μg/ml. Danthrone at a concentration of 10μg/ml and acetone (0.1%) similarly enhanced IL-1 secretion from human MO measured by both methods. Our results demonstrate a differential, dose-dependent inhibition of cytokine secretion from human MO by anthralin. The present data provide evidence that the anti-inflammatory and anti- proliferative activity of anthralin may at least in part be due to its inhibitory effect on pro-inflammatory cytokine secretion by MO. Citing Literature Volume136, Issue4April 1997Pages 542-547 RelatedInformation
Publication Year: 1997
Publication Date: 1997-04-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 31
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