Abstract: In studies concerned with the in GLYJ fate of biologically active compounds. the detectability of the parent compound and its metabolites is crucial. The high biological potencies of brain oligopeptides necessitate detection at a level of less than I ng. implicating very sensitive analytical procedures. The bioassay offers a straightforward approach to follow the appearance and disappearance of biological activity in various tissues with time. However, bioassay, by its nature, detects only rather large changes in biological activity. The specificity and sensitivity of the particular bioassay have to be considered. In behavioral assays a routine measurement of biological activity is hardly feasible. Oligopeptide concentrations obtanned by radioimmunoassay ideally reflect those of the parent molecule. However. the specificity of the assay does not always allow differentiation between the original oligopeptide and closely resembling metabolites, precursor molecules or structurally analogous peptides. Bioassay and radioimmunoassay provide little or no information on metabolic pathways.
Publication Year: 1975
Publication Date: 1975-01-01
Language: en
Type: article
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