Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2072616898', 'doi': 'https://doi.org/10.1046/j.1365-313x.1997.11030613.x', 'title': 'The green fluorescent protein as a marker to visualize plant mitochondria <i>in vivo</i>', 'display_name': 'The green fluorescent protein as a marker to visualize plant mitochondria <i>in vivo</i>', 'publication_year': 1997, 'publication_date': '1997-03-01', 'ids': {'openalex': 'https://openalex.org/W2072616898', 'doi': 'https://doi.org/10.1046/j.1365-313x.1997.11030613.x', 'mag': '2072616898', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9107047'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1046/j.1365-313x.1997.11030613.x', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313X.1997.11030613.x', 'source': {'id': 'https://openalex.org/S67862205', 'display_name': 'The Plant Journal', 'issn_l': '0960-7412', 'issn': ['0960-7412', '1365-313X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313X.1997.11030613.x', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5031991453', 'display_name': 'Rainer H. Köhler', 'orcid': 'https://orcid.org/0000-0001-6269-6590'}, 'institutions': [{'id': 'https://openalex.org/I205783295', 'display_name': 'Cornell University', 'ror': 'https://ror.org/05bnh6r87', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I205783295']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Rainer H. Köhler', 'raw_affiliation_strings': ['Section of Genetics and Development, Cornell University, Ithaca, NY 14853 USA'], 'affiliations': [{'raw_affiliation_string': 'Section of Genetics and Development, Cornell University, Ithaca, NY 14853 USA', 'institution_ids': ['https://openalex.org/I205783295']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5066282020', 'display_name': 'Warren R. Zipfel', 'orcid': 'https://orcid.org/0000-0003-2640-329X'}, 'institutions': [{'id': 'https://openalex.org/I205783295', 'display_name': 'Cornell University', 'ror': 'https://ror.org/05bnh6r87', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I205783295']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Warren R. Zipfel', 'raw_affiliation_strings': ['Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA'], 'affiliations': [{'raw_affiliation_string': 'Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA', 'institution_ids': ['https://openalex.org/I205783295']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5111539887', 'display_name': 'Watt W. Webb', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I205783295', 'display_name': 'Cornell University', 'ror': 'https://ror.org/05bnh6r87', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I205783295']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Watt W. Webb', 'raw_affiliation_strings': ['Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA'], 'affiliations': [{'raw_affiliation_string': 'Department of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA', 'institution_ids': ['https://openalex.org/I205783295']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5044430961', 'display_name': 'Maureen R. Hanson', 'orcid': 'https://orcid.org/0000-0001-8141-3058'}, 'institutions': [{'id': 'https://openalex.org/I4210118410', 'display_name': 'Institut thématique Génétique, génomique et bioinformatique', 'ror': 'https://ror.org/01rfstj90', 'country_code': 'FR', 'type': 'government', 'lineage': ['https://openalex.org/I154526488', 'https://openalex.org/I4210118410']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Maureen R. Hanson', 'raw_affiliation_strings': ['Section of Genetics and Development, Biotechnology Building, and'], 'affiliations': [{'raw_affiliation_string': 'Section of Genetics and Development, Biotechnology Building, and', 'institution_ids': ['https://openalex.org/I4210118410']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 4940, 'currency': 'USD', 'value_usd': 4940, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 5.016, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 240, 'citation_normalized_percentile': {'value': 0.999969, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '11', 'issue': '3', 'first_page': '613', 'last_page': '621'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10303', 'display_name': 'Photosynthetic Processes and Mechanisms', 'score': 0.9935, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10303', 'display_name': 'Photosynthetic Processes and Mechanisms', 'score': 0.9935, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10301', 'display_name': 'Mitochondrial Function and Pathology', 'score': 0.9856, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/autofluorescence', 'display_name': 'Autofluorescence', 'score': 0.7262385}, {'id': 'https://openalex.org/keywords/plant-cell', 'display_name': 'Plant cell', 'score': 0.53992504}], 'concepts': [{'id': 'https://openalex.org/C142613039', 'wikidata': 'https://www.wikidata.org/wiki/Q272983', 'display_name': 'Green fluorescent protein', 'level': 3, 'score': 0.90063083}, {'id': 'https://openalex.org/C179687394', 'wikidata': 'https://www.wikidata.org/wiki/Q4826281', 'display_name': 'Autofluorescence', 'level': 3, 'score': 0.7262385}, {'id': 'https://openalex.org/C28859421', 'wikidata': 'https://www.wikidata.org/wiki/Q39572', 'display_name': 'Mitochondrion', 'level': 2, 'score': 0.7111454}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.6502803}, {'id': 'https://openalex.org/C2776879804', 'wikidata': 'https://www.wikidata.org/wiki/Q7630919', 'display_name': 'Subcellular localization', 'level': 3, 'score': 0.6045284}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.58326596}, {'id': 'https://openalex.org/C183873130', 'wikidata': 'https://www.wikidata.org/wiki/Q30869', 'display_name': 'Nucleolus', 'level': 3, 'score': 0.58129007}, {'id': 'https://openalex.org/C140051345', 'wikidata': 'https://www.wikidata.org/wiki/Q7251501', 'display_name': 'Protein subcellular localization prediction', 'level': 3, 'score': 0.56371456}, {'id': 'https://openalex.org/C190062978', 'wikidata': 'https://www.wikidata.org/wiki/Q79899', 'display_name': 'Cytoplasm', 'level': 2, 'score': 0.54391295}, {'id': 'https://openalex.org/C156203801', 'wikidata': 'https://www.wikidata.org/wiki/Q1056194', 'display_name': 'Plant cell', 'level': 3, 'score': 0.53992504}, {'id': 'https://openalex.org/C102568950', 'wikidata': 'https://www.wikidata.org/wiki/Q127702', 'display_name': 'Vacuole', 'level': 3, 'score': 0.49041554}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.38427427}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.23022324}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.13277358}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C62520636', 'wikidata': 'https://www.wikidata.org/wiki/Q944', 'display_name': 'Quantum mechanics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D008928', 'descriptor_name': 'Mitochondria', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D002478', 'descriptor_name': 'Cells, Cultured', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017930', 'descriptor_name': 'Genes, Reporter', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005819', 'descriptor_name': 'Genetic Markers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008164', 'descriptor_name': 'Luminescent Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D018613', 'descriptor_name': 'Microscopy, Confocal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018613', 'descriptor_name': 'Microscopy, Confocal', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': False}, {'descriptor_ui': 'D008928', 'descriptor_name': 'Mitochondria', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014026', 'descriptor_name': 'Nicotiana', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014026', 'descriptor_name': 'Nicotiana', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D030821', 'descriptor_name': 'Plants, Genetically Modified', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D030821', 'descriptor_name': 'Plants, Genetically Modified', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D010947', 'descriptor_name': 'Plants, Toxic', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017354', 'descriptor_name': 'Point Mutation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D012231', 'descriptor_name': 'Rhizobium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014162', 'descriptor_name': 'Transfection', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': False}, {'descriptor_ui': 'D014162', 'descriptor_name': 'Transfection', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1046/j.1365-313x.1997.11030613.x', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313X.1997.11030613.x', 'source': {'id': 'https://openalex.org/S67862205', 'display_name': 'The Plant Journal', 'issn_l': '0960-7412', 'issn': ['0960-7412', '1365-313X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9107047', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1046/j.1365-313x.1997.11030613.x', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313X.1997.11030613.x', 'source': {'id': 'https://openalex.org/S67862205', 'display_name': 'The Plant Journal', 'issn_l': '0960-7412', 'issn': ['0960-7412', '1365-313X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/3', 'score': 0.83, 'display_name': 'Good health and well-being'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W4389024241', 'https://openalex.org/W4366498163', 'https://openalex.org/W4282922129', 'https://openalex.org/W4205775722', 'https://openalex.org/W3215969703', 'https://openalex.org/W2406531951', 'https://openalex.org/W2385196349', 'https://openalex.org/W2384565783', 'https://openalex.org/W2351398203', 'https://openalex.org/W1966748065'], 'abstract_inverted_index': {'To': [0], 'determine': [1], 'how': [2], 'to': [3, 157, 166], 'utilize': [4], 'the': [5, 68, 82, 119, 135, 142, 184, 211], 'green': [6, 65], 'fluorescent': [7], 'protein': [8, 173], '(GFP)': [9], 'as': [10, 17, 57, 80, 174], 'a': [11, 18, 36, 85, 90, 98, 114, 179], 'marker': [12], 'for': [13, 20], 'subcellular': [14, 116], 'localization': [15, 38, 117], 'and': [16, 28, 34, 74, 97, 150, 189, 196, 213], 'label': [19], 'plant': [21, 194, 199], 'mitochondria': [22, 167, 188], 'in': [23, 51, 64, 72, 121, 134, 168, 192], 'vivo,': [24], 'transgenic': [25], 'suspension': [26, 58], 'cells': [27, 52, 59, 73, 122, 128, 169, 195, 205], 'tobacco': [29], 'plants': [30], 'expressing': [31, 123, 129, 170], 'GFP': [32, 44, 71, 103, 124], 'with': [33, 53, 76, 178], 'without': [35], 'mitochondrial': [37, 190], 'signal': [39], 'were': [40], 'generated.': [41], 'The': [42], 'first': [43], 'form': [45, 101], 'used,': [46], 'GFP1,': [47], 'is': [48, 138, 145, 163], 'easily': [49], 'observable': [50], 'low': [54], 'autofluorescence,': [55, 78], 'such': [56, 79], 'or': [60, 108, 125], 'trichomes,': [61], 'but': [62, 137], 'masked': [63], 'tissue.': [66], 'For': [67], 'visualization': [69], 'of': [70, 84, 102, 118, 187, 203, 215], 'tissues': [75, 197], 'high': [77], 'leaf,': [81], 'use': [83], 'very': [86], 'strong': [87], 'promoter': [88], '(35S35SAMV),': [89], 'highly': [91], 'expressed': [92], 'modified': [93], 'mGFP4': [94], 'coding': [95], 'region': [96], 'brighter': [99], 'mutant': [100], '(S65T)': [104], 'was': [105], 'necessary.': [106], 'Confocal': [107], 'two-photon': [109], 'laser': [110], 'scanning': [111], 'microscopy': [112], 'reveal': [113], 'distinct': [115], 'fluorescence': [120, 132, 162], 'coxIVGFP.': [126], 'In': [127, 160], 'untargeted': [130], 'GFP,': [131], 'accumulates': [133], 'nucleoplasm': [136], 'also': [139], 'distributed': [140], 'throughout': [141, 198], 'cytoplasm.': [143], 'It': [144], 'excluded': [146], 'from': [147, 151], 'vacuoles,': [148], 'nucleoli': [149], 'round': [152], 'bodies': [153], 'that': [154], 'are': [155], 'likely': [156], 'be': [158], 'leucoplasts.': [159], 'contrast,': [161], 'localized': [164], 'specifically': [165], 'coxIVGFP': [171], 'fusion': [172], 'shown': [175], 'by': [176], 'co-localization': [177], 'mitochondrial-specific': [180], 'dye.': [181], 'This': [182], 'permits': [183], 'direct': [185], 'observation': [186], 'movements': [191], 'living': [193], 'development.': [200], 'Three-dimensional': [201], 'reconstruction': [202], 'individual': [204], 'can': [206], 'give': [207], 'additional': [208], 'information': [209], 'about': [210], 'distribution': [212], 'numbers': [214], 'mitochondria.': [216]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2072616898', 'counts_by_year': [{'year': 2024, 'cited_by_count': 6}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 6}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 8}, {'year': 2019, 'cited_by_count': 6}, {'year': 2018, 'cited_by_count': 7}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 7}], 'updated_date': '2025-01-08T11:24:26.130682', 'created_date': '2016-06-24'}