Title: Study on the subcellular localization of eIF-5A
Abstract: Objective:To study the subcellular localization of eIF-5A, and find the clues for its function.Methods:eIF-5A subcellular localization was examined by laser confocal microscope, using the approaches of immunofluorescent staining and GFP-tagging.Results:The localization of eIF-5A was primarily cytoplasmic by indirect immunofluorescent staining. The results of transient transfection showed that GFP-eIF-5A was distributed over whole cell at the beginning of its expression, but was gradually concentrated in cytoplasm. The mutation of lysine 50→alanine affected its distribution in the cytoplasm.Conclusion:eIF-5A might be a nucleocytoplasmic shuttle protein, and its subcellular localization was a dynamic process and hypusine could affect the function of eIF-5A through swaying its subcellular localization.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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