Title: Rumen Concentrations of Volatile Fatty Acids as Affected by Feeding Frequency
Abstract: animal is adjusted to a pelleted feed of 1A in. in diameter, commonly referred to as rabbit-or poultry-sized pellets.These pellets are fed 2 wk.prior to the operation and 1.5 wk.following the operation, after which the animal is adjusted to alfalfa hay gradually for a few days.At the end of 2 wk.following the operation, the animal can be used in experimental trials to collect samples of forage.A large canvas bag directly under the eannula permits accumulation of the sample.A No. 5 rubber stopper is placed in the open end of the caanula for closure.Animals with a steel esophageal fistula eannula have been used in research for 2 yr.