Activation of Muscle-specific Receptor Tyrosine Kinase and Binding to Dystroglycan Are Regulated by Alternative mRNA Splicing of Agrin

Name: Work Video:
Duration: 3 min 30 s
Patrick Scotton; Dorothée Bleckmann; Michael Stebler; Francesca Sciandra; Andrea Brancaccio; Thomas Meier; Jörg Stetefeld; Markus A. Rüegg
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'abstract_inverted_index': {'Agrin': [0, 19, 160, 179, 320, 370, 3056], 'induces': [1, 161, 1983, 2001, 4093], 'the': [2, 8, 15, 38, 69, 81, 86, 92, 125, 150, 153, 162, 168, 175, 198, 229, 241, 246, 252, 285, 310, 313, 338, 374, 386, 425, 437, 442, 447, 665, 668, 694, 749, 820, 840, 843, 852, 858, 991, 996, 999, 1008, 1139, 1318, 1356, 1459, 1463, 1467, 1475, 1481, 1494, 1679, 1693, 1705, 1711, 1714, 1720, 1731, 1767, 1787, 1806, 1852, 1856, 1860, 1931, 1936, 1953, 1960, 1974, 1987, 1993, 2008, 2033, 2043, 2076, 2081, 2094, 2099, 2103, 2107, 2124, 2134, 2138, 2163, 2166, 2171, 2174, 2197, 2202, 2215, 2233, 2335, 2340, 2346, 2353, 2359, 2362, 2372, 2405, 2451, 2552, 2569, 2604, 2607, 2642, 2665, 2686, 2689, 2707, 2710, 2734, 2850, 2861, 2880, 2884, 2902, 2916, 2997, 3033, 3153, 3189, 3263, 3283, 3290, 3317, 3381, 3424, 3453, 3610, 3740, 3770, 3796, 3804, 3807, 3811, 3814, 3821, 3827, 3836, 3858, 3887, 3954, 3969, 3973, 3988, 4005, 4021, 4025, 4047, 4089, 4123, 4131, 4201, 4211, 4214, 4324, 4331, 4334, 4348, 4351, 4372, 4376, 4392, 4414, 4421, 4427, 4430, 4437, 4444, 4453, 4480, 4516, 4550, 4569, 4594], 'aggregation': [3, 163, 1246, 4095, 4179, 4486], 'of': [4, 54, 59, 76, 164, 214, 219, 236, 377, 388, 449, 480, 544, 662, 667, 691, 741, 745, 751, 755, 837, 842, 855, 974, 1007, 1206, 1216, 1239, 1320, 1478, 1636, 1675, 1681, 1778, 1790, 1805, 1859, 1868, 1871, 1942, 1946, 1955, 1959, 1979, 2054, 2075, 2110, 2116, 2140, 2165, 2196, 2214, 2218, 2227, 2238, 2247, 2264, 2361, 2606, 2823, 2849, 2913, 2918, 2923, 2975, 2999, 3152, 3183, 3215, 3252, 3289, 3320, 3329, 3359, 3401, 3444, 3486, 3585, 3609, 3783, 3803, 3813, 3829, 3862, 3874, 3902, 3968, 3983, 3990, 4020, 4027, 4050, 4058, 4125, 4130, 4154, 4213, 4333, 4347, 4403, 4410, 4423, 4439, 4475, 4508, 4553, 4562, 4568, 4598, 4605], 'postsynaptic': [5, 165, 335, 450, 474, 539, 612], 'proteins': [6, 166, 1539, 1873, 2373, 2438, 2817, 3133, 3420, 3471, 3557, 4246], 'at': [7, 27, 91, 139, 149, 167, 187, 251, 299, 309, 337, 478, 839, 995, 1692, 1710, 1719, 1766, 1935, 1986, 2080, 2126, 2136, 2170, 2442, 2464, 2949, 2989, 3020, 3023, 3032, 3128, 3195, 3247, 3325, 3395, 3531, 3603, 3706, 3799, 3823, 4101], 'neuromuscular': [9, 169, 339, 347], 'junction': [10, 170, 340], '(NMJ).': [11, 171, 341], 'This': [12, 172, 421, 1122, 4083, 4304, 4360], 'activity': [13, 123, 173, 283, 383, 1352, 1646, 4464, 4474], 'requires': [14, 138, 174, 298, 4180], 'receptor-tyrosine': [16, 176, 1358], 'kinase': [17, 177, 1359], 'MuSK.': [18, 178], 'isoforms': [20, 48, 180, 208, 831, 1222, 1996], 'differ': [21, 50, 181, 210, 2016], 'in': [22, 37, 51, 85, 182, 197, 211, 245, 432, 452, 541, 549, 614, 664, 693, 828, 846, 978, 990, 1067, 1071, 1075, 1126, 1130, 1138, 1195, 1199, 1212, 1220, 1247, 1323, 1466, 1535, 2005, 2032, 2062, 2098, 2131, 2240, 2304, 2331, 2450, 2479, 2930, 3051, 3067, 3103, 3160, 3188, 3223, 3365, 3410, 3448, 3512, 3559, 3580, 3712, 3826, 3886, 4173, 4254, 4268, 4289, 4371, 4386, 4405, 4443, 4450, 4591], 'short': [23, 183], 'amino': [24, 70, 108, 146, 184, 230, 268, 306, 1233, 1735, 1932, 1966, 1971, 1989, 2029, 2056, 2073, 2119, 2167, 2193, 4162, 4424, 4566, 4583], 'acid': [25, 88, 147, 185, 248, 307, 1081, 1234, 1707, 1716, 1736, 1933, 1990, 2078, 2120, 2168, 4163], 'stretches': [26, 186], 'two': [28, 39, 141, 188, 199, 301, 1204], 'sites,': [29, 189], 'called': [30, 190, 1069, 1193], 'A': [31, 151, 191, 311, 1070, 1694, 4284], 'and': [32, 58, 96, 144, 152, 192, 218, 256, 304, 312, 333, 424, 476, 607, 640, 690, 906, 981, 984, 1062, 1073, 1132, 1145, 1197, 1209, 1316, 1497, 1543, 1607, 1695, 1713, 1769, 1802, 1969, 1976, 2007, 2019, 2112, 2173, 2260, 2274, 2323, 2358, 2397, 2425, 2474, 2492, 2524, 2558, 2573, 2587, 2600, 2621, 2672, 2684, 2705, 2719, 2725, 2748, 2780, 2797, 2799, 2837, 2879, 2906, 2973, 3008, 3038, 3089, 3101, 3122, 3168, 3177, 3229, 3242, 3271, 3286, 3323, 3547, 3569, 3592, 3649, 3691, 3703, 3724, 3750, 3760, 3816, 3818, 3825, 3879, 3972, 4003, 4015, 4032, 4076, 4078, 4170, 4247, 4256, 4381, 4397, 4469, 4596], 'B,': [33, 193, 3459], 'that': [34, 80, 134, 145, 194, 240, 294, 305, 427, 439, 602, 849, 910, 1085, 1456, 1473, 1489, 1678, 1703, 1783, 1867, 2028, 2067, 2093, 2162, 2192, 2221, 2811, 2825, 2843, 3144, 3406, 4073, 4092, 4165, 4192, 4251, 4362, 4604], 'are': [35, 195, 599, 1054, 1784, 1792, 2012, 2854, 3820], 'localized': [36, 196], 'most': [40, 200, 2009, 2095], 'C-terminal': [41, 201, 1701, 4069], 'laminin': [42, 202, 353, 1057], 'G': [43, 203, 1058], '(LG)': [44, 204, 1059], 'domains.': [45, 205], 'Importantly,': [46, 206], 'agrin': [47, 115, 207, 275, 440, 533, 603, 663, 742, 821, 860, 912, 975, 989, 1127, 1350, 1448, 1468, 1479, 1637, 1682, 1791, 1811, 2034, 2069, 2141, 2181, 2203, 2336, 2364, 2437, 2956, 3000, 3556, 3710, 4039, 4059, 4278, 4300, 4431, 4440, 4476], 'greatly': [49, 209], 'their': [52, 212, 2178, 4484], 'activities': [53, 75, 213, 235], 'inducing': [55, 215, 446], 'MuSK': [56, 100, 121, 216, 260, 281, 1360, 1453, 1457, 2063, 2157, 2219, 3045, 4181, 4253, 4266, 4383, 4404, 4418, 4432, 4445, 4472, 4514, 4555, 4617], 'phosphorylation': [57, 101, 122, 217, 261, 282, 4267, 4384, 4402, 4433, 4446, 4473], 'binding': [60, 135, 220, 295, 1089, 1464, 1482, 1635, 1680, 2182, 2944, 3140, 3502, 3525, 3785, 4036], 'to': [61, 136, 221, 296, 331, 384, 436, 537, 609, 851, 913, 987, 1452, 1483, 1529, 1638, 1683, 1724, 1851, 1866, 1948, 2026, 2058, 2150, 2183, 2201, 2345, 2408, 2550, 2561, 2567, 2641, 2732, 2809, 3136, 3282, 3294, 3651, 3788, 3899, 4033, 4087, 4195, 4400, 4416, 4429, 4574, 4611], 'α-dystroglycan.': [62, 222, 2184], 'By': [63, 223], 'using': [64, 224, 2256, 2339, 2723, 3004, 3589, 3779, 3906, 3978], 'site-directed': [65, 225, 2052, 2821], 'mutagenesis,': [66, 226], 'we': [67, 227, 2160, 4134, 4148, 4183, 4435, 4557, 4579], 'characterized': [68, 228], 'acids': [71, 109, 231, 269, 1967, 1972, 2030, 2057, 2074, 2194, 4425, 4567, 4584], 'important': [72, 232, 1785], 'for': [73, 98, 233, 258, 328, 748, 1244, 1317, 1643, 1775, 1786, 2156, 2439, 2461, 2476, 2571, 2603, 2763, 2959, 2986, 3061, 3125, 3192, 3244, 3677, 3727, 3995, 4200], 'these': [74, 234, 1217], 'agrin.': [77, 237, 1779], 'We': [78, 131, 238, 291, 2049, 2065, 2090, 4160], 'find': [79, 239, 2066, 2092, 2161], 'conserved': [82, 242, 4565], 'tripeptide': [83, 243, 2104], 'asparagineglutamate-isoleucine': [84, 244], 'eight-amino': [87, 247, 1715, 2077], 'long': [89, 249, 1082, 1235, 1708, 1717, 2865], 'insert': [90, 250, 1083, 1123, 1150, 1241, 1709, 1718, 1934, 2045, 2079, 2101, 3852, 3889, 4571], 'B-site': [93, 253, 1721, 1770, 1937, 2082, 2175, 4415], 'is': [94, 254, 321, 430, 441, 535, 604, 976, 985, 1002, 1124, 1201, 1242, 1353, 1458, 1480, 1490, 1640, 1685, 1771, 1863, 1938, 2087, 2102, 2148, 4085, 4208], 'necessary': [95, 255, 1642], 'sufficient': [97, 257, 536, 608], 'full': [99, 259], 'activity.': [102, 262, 2204], 'However,': [103, 263, 1447], 'even': [104, 264, 4307, 4505], 'if': [105, 265], 'all': [106, 266, 2072, 2812, 4038, 4581], 'eight': [107, 267, 4582], 'were': [110, 270, 2083, 2277, 2302, 2328, 2374, 2388, 2433, 2448, 2468, 2484, 2545, 2575, 2609, 2638, 2721, 2736, 2765, 2793, 2814, 2818, 2833, 2891, 2897, 3049, 3058, 3098, 3134, 3186, 3199, 3236, 3363, 3421, 3438, 3472, 3504, 3553, 3563, 3613, 3675, 3717, 3743, 3767, 3777, 3833, 3890, 3951, 3976, 4041, 4166, 4572], 'replaced': [111, 271, 2084, 4580], 'by': [112, 272, 1005, 1203, 1355, 1504, 1688, 1799, 2085, 2252, 2261, 2376, 2403, 2679, 2700, 2820, 2835, 2876, 2893, 2909, 2963, 3003, 3211, 3304, 3426, 3465, 3490, 3528, 3769, 4274, 4461, 4585], 'alanines,': [113, 273], 'this': [114, 274, 1149, 4363], 'mutant': [116, 276, 2070], 'still': [117, 277, 2088, 4395, 4615], 'has': [118, 278, 1531, 2152], 'significantly': [119, 279, 4389], 'higher': [120, 280, 4390], 'than': [124, 284, 1730, 4603], 'splice': [126, 155, 286, 315, 1052, 1065, 1191, 1812, 1957, 2142, 2198, 3851, 3888, 4127, 4441, 4477], 'version': [127, 287, 2213], 'lacking': [128, 288, 1734], 'any': [129, 289, 4513], 'insert.': [130, 290], 'also': [132, 292, 658, 1532, 1686, 2000, 2047, 2091, 4135, 4542], 'show': [133, 293], 'α-dystroglycan': [137, 297, 1530, 1639, 1684, 1725, 3487, 3509, 3625], 'least': [140, 300, 816], 'LG': [142, 302, 1476, 1537, 1869], 'domains': [143, 303, 1060, 1477, 1870], 'inserts': [148, 308, 1737, 2169, 4128], 'B': [154, 314, 1194, 1696, 2100], 'sites': [156, 316, 818, 1053, 2199, 3786], 'negatively': [157, 317], 'affect': [158, 318], 'binding.': [159, 319], 'a': [322, 660, 738, 752, 920, 926, 1079, 1087, 1485, 1500, 1700, 1772, 2002, 2117, 2129, 2211, 2495, 2562, 2941, 3028, 3137, 3386, 3522, 3635, 3780, 3800, 4064, 4067, 4097, 4151, 4186, 4387, 4560], 'heparan': [323], 'sulfate': [324], 'proteoglycan': [325], 'best': [326], 'known': [327, 1794, 4086], 'its': [329, 382, 1644, 1949], 'function': [330, 423, 1472, 2036], 'induce': [332, 385, 538, 4512], 'maintain': [334], 'specializations': [336, 475], '3The': [342], 'abbreviations': [343], 'used': [344, 2051, 2546, 2566, 2602, 2731, 2762, 2808, 3845, 4150, 4345], 'are:': [345], 'NMJ,': [346, 997], 'junction;': [348], 'AChR,': [349], 'acetylcholine': [350, 389], 'receptors;': [351], 'LG,': [352], 'G;': [354], 'PBS,': [355], 'phosphate-buffered': [356], 'saline;': [357], 'HRP,': [358], 'horseradish': [359], 'peroxidase;': [360], 'PDB,': [361], 'Protein': [362], 'Data': [363, 3832], 'Bank;': [364], 'MuSK,': [365], 'muscle-specific': [366, 1357], 'receptor': [367, 1469], 'tyrosine': [368], 'kinase.': [369], 'was': [371, 2208, 2244, 2250, 2356, 2366, 2401, 2677, 2698, 2807, 2826, 2859, 2874, 2886, 2938, 2957, 3001, 3015, 3041, 3265, 3279, 3292, 3299, 3309, 3331, 3383, 3393, 3463, 3488, 3587, 3597, 3626, 3699, 3846, 3854, 3897, 3985, 4012, 4061, 4287, 4312, 4344, 4378, 4394, 4456, 4465, 4600], 'purified': [372, 2224, 2955], 'from': [373, 1499, 1809, 1981, 2145, 2225, 3423, 3461, 4024, 4063, 4368], 'electric': [375], 'organ': [376], 'Torpedo': [378], 'californica': [379], 'based': [380, 3856, 4209], 'on': [381, 392, 2390, 2486, 2846, 2856, 2860, 2940, 3017, 3311, 3521, 3599, 3634, 3857, 3953, 3987, 4210], 'clustering': [387], 'receptors': [390], '(AChRs)': [391], 'cultured': [393, 2303, 4269, 4406], 'muscle': [394, 616, 2267, 3624], 'cells': [395, 980, 1134, 1137, 2259, 2300, 2447, 2483, 4031], '(1Nitkin': [396], 'R.M.': [397], 'Smith': [398, 700, 721, 1381], 'M.A.': [399, 622, 701, 722, 774, 872, 887, 958, 1038, 1152, 1250, 1304, 1658, 1747, 1831, 2287, 2512, 4109, 4492, 4529], 'Magill': [400], 'C.': [401, 674, 785, 797, 1105, 1382, 1412, 2651], 'Fallon': [402], 'J.R.': [403, 496, 520, 642, 935, 1015, 1334], 'Yao': [404], 'Y.M.M.': [405], 'Wallace': [406], 'B.G.': [407], 'McMahan': [408, 1163, 1261], 'U.J.': [409, 455, 1164, 1262], 'J.': [410, 586, 702, 760, 787, 873, 893, 895, 897, 936, 1016, 1108, 1181, 1305, 1414, 1577, 1598, 1614, 1616, 1621, 1748, 1815, 1883, 1914, 2288, 2513, 3344, 3658, 3923, 4110, 4493, 4530], 'Cell': [411, 587, 703, 874, 937, 960, 1017, 1040, 1306, 1622, 2272, 2289, 2514, 4111, 4494, 4531], 'Biol.': [412, 461, 588, 704, 875, 938, 1018, 1109, 1307, 1623, 1749, 1915, 2290, 2515, 3939, 4112, 4495, 4532], '1987;': [413], '105:': [414], '2471-2478Crossref': [415], 'PubMed': [416, 465, 506, 527, 573, 592, 635, 651, 685, 708, 732, 779, 810, 879, 901, 942, 965, 1022, 1045, 1101, 1119, 1172, 1186, 1270, 1294, 1311, 1344, 1400, 1442, 1523, 1561, 1581, 1602, 1627, 1668, 1759, 1844, 1893, 1925, 2294, 2519, 2540, 2659, 3350, 3644, 3669, 3944, 4116, 4238, 4499, 4536], 'Scopus': [417, 466, 507, 528, 574, 593, 636, 652, 686, 709, 733, 780, 811, 880, 902, 943, 966, 1023, 1046, 1173, 1271, 1295, 1312, 1345, 1401, 1443, 1524, 1562, 1582, 1603, 1628, 1669, 1760, 1845, 1894, 1926, 2295, 2520, 2541, 2660, 3351, 3645, 3670, 3945, 4117, 4239, 4500, 4537], '(369)': [418], 'Google': [419, 468, 509, 530, 576, 595, 638, 654, 688, 711, 735, 782, 813, 882, 904, 945, 968, 1025, 1048, 1102, 1120, 1175, 1187, 1273, 1297, 1314, 1347, 1403, 1445, 1526, 1564, 1584, 1605, 1630, 1671, 1762, 1847, 1896, 1928, 2297, 2522, 2543, 2662, 3353, 3647, 3672, 3947, 4119, 4241, 4502, 4539], 'Scholar).': [420, 469, 531, 596, 655, 736, 814, 969, 1049, 1121, 1188, 1348, 1446, 1527, 1631, 1672, 1763, 2298, 3354, 3673, 3948, 4120, 4503], 'biological': [422], 'fact': [426], 'agrin-like': [428], 'immunoreactivity': [429], 'high': [431, 2003, 2943, 3139, 3524, 4352, 4365], 'motor': [433, 443, 982], 'neurons': [434, 983, 1006, 1131], 'led': [435, 1850, 2024], 'hypothesis': [438, 2191], 'neuron-derived': [444], 'factor': [445], 'formation': [448, 666, 1319], 'structures': [451, 540, 613, 3873], 'vivo': [453, 550, 1324], '(2McMahan': [454], 'Cold': [456], 'Spring': [457], 'Harb.': [458], 'Symp.': [459], 'Quant.': [460], '1990;': [462, 4235], '55:': [463], '407-418Crossref': [464], '(580)': [467], 'Indeed,': [470], 'agrin-deficient': [471], 'mice': [472], 'lack': [473, 1148], 'die': [477], 'birth': [479], 'respiratory': [481], 'failure': [482], '(3Gautam': [483], 'M.': [484, 556, 583, 868, 870, 952, 1032, 1276, 1300, 1648, 1741, 1821, 2283, 2508, 3342, 3925, 4105, 4221, 4488, 4525], 'Noakes': [485], 'P.G.': [486, 1901], 'Moscoso': [487], 'L.': [488], 'Rupp': [489, 679, 1281], 'F.': [490, 680, 1282, 1612, 2529], 'Scheller': [491, 1095, 1285], 'R.H.': [492, 1096, 1286], 'Merlie': [493], 'J.P.': [494, 581], 'Sanes': [495, 519, 934, 1014, 1333], 'Cell.': [497, 723, 1391, 1433, 1884], '1996;': [498, 1098, 1111, 1392, 1434, 1660], '85:': [499, 1393, 1435], '525-535Abstract': [500], 'Full': [501, 503, 630, 632, 727, 729, 1114, 1116, 1169, 1267, 1291, 1339, 1341, 1395, 1397, 1437, 1439, 1663, 1665, 1754, 1756, 1839, 1841, 1888, 1890, 1920, 1922, 2537], 'Text': [502, 504, 631, 633, 728, 730, 1115, 1117, 1170, 1268, 1292, 1340, 1342, 1396, 1398, 1438, 1440, 1664, 1666, 1755, 1757, 1840, 1842, 1889, 1891, 1921, 1923, 2538], 'PDF': [505, 634, 731, 1118, 1171, 1269, 1293, 1343, 1399, 1441, 1667, 1758, 1843, 1892, 1924, 2539], '(802)': [508], 'Scholar,': [510, 577, 712, 783, 883, 946, 1026, 1103, 1176, 1274, 1298, 1404, 1565, 1897], '4Lin': [511], 'W.': [512, 891, 1107, 1278, 1571, 1592, 1905], 'Burgess': [513], 'R.W.': [514, 931, 1011, 1326, 3919], 'Dominguez': [515], 'B.': [516, 560, 954, 1034, 1656, 1745, 3450, 4219], 'Pfaff': [517], 'S.L.': [518], 'Lee': [521], 'K.F.': [522], 'Nature.': [523, 775, 1519, 1557, 3640], '2001;': [524, 589, 648, 682, 776, 962, 1042, 1624], '410:': [525], '1057-1064Crossref': [526], '(452)': [529], 'Moreover,': [532, 737], 'alone': [534], 'nerve-free': [542], 'regions': [543], 'adult,': [545], 'fully': [546], 'innervated': [547], 'muscles': [548], '(5Jones': [551], 'G.': [552, 579, 766, 950, 1030, 1160, 1258, 1875], 'Meier': [553], 'T.': [554, 585, 789, 1825, 1877, 1899, 3340, 3656, 3933], 'Lichtsteiner': [555], 'Witzemann': [557], 'V.': [558, 1650], 'Sakmann': [559], 'Brenner': [561], 'H.R.': [562], 'Proc.': [563, 800, 3659, 4228], 'Natl.': [564, 801, 3660, 4229], 'Acad.': [565, 802, 3661, 4230], 'Sci.': [566, 803, 3662, 4231], 'U.': [567, 772, 804, 889, 3663, 4232], 'S.': [568, 764, 793, 805, 956, 1036, 1158, 1256, 1372, 1420, 1610, 1829, 3664, 4233], 'A.': [569, 806, 1573, 1594, 1654, 1743, 1909, 2527, 3338, 3412, 3665, 4234], '1997;': [570, 876, 2656], '94:': [571], '2654-2659Crossref': [572], '(133)': [575], '8Bezakova': [578], 'Helm': [580], 'Francolini': [582], 'Lomo': [584], '153:': [590], '1441-1452Crossref': [591], '(45)': [594, 1846], 'These': [597, 2021], 'data': [598, 2187], 'strong': [600, 4609], 'evidence': [601, 657, 1676], 'both': [605, 3869], 'required': [606, 986], 'generate': [610, 2551], 'functional': [611, 1321], 'skeletal': [615, 3623], '(for': [617], 'reviews': [618], 'see': [619, 4541], 'Refs.': [620], '9Ruegg': [621], 'Bixby': [623], 'J.L.': [624], 'Trends': [625, 2532], 'Neurosci.': [626, 647, 961, 1041, 1182], '1998;': [627, 1751, 3941], '21:': [628], '22-27Abstract': [629], '(160)': [637], 'Scholar': [639], '10Sanes': [641], 'Lichtman': [643, 1331], 'J.W.': [644, 1332], 'Nat.': [645], 'Rev.': [646], '2:': [649], '791-805Crossref': [650], '(827)': [653], 'Recent': [656], 'suggests': [659], 'role': [661], 'immunological': [669], 'synapse': [670], '(11Khan': [671], 'A.A.': [672], 'Bose': [673], 'Yam': [675], 'L.S.': [676], 'Soloski': [677], 'M.J.': [678], 'Science.': [681], '292:': [683], '1681-1686Crossref': [684], '(152)': [687], 'Scholar)': [689, 905, 1315, 1848, 2544, 2663], 'synapses': [692], 'brain': [695, 1009], '(12Hoover': [696], 'C.L.': [697], 'Hilgenberg': [698], 'L.G.': [699, 714], '2003;': [705, 898], '161:': [706], '923-932Crossref': [707], '(28)': [710], '13Hilgenberg': [713], 'Su': [715], 'H.': [716, 718, 768, 3654, 4223], 'Gu': [717], "O'Dowd": [719], 'D.K.': [720], '2006;': [724], '125:': [725], '359-369Abstract': [726], '(103)': [734], 'miniaturized': [739, 4152], 'form': [740, 754, 973, 1001, 4153], 'might': [743, 2037], 'be': [744, 914, 2040, 4088], 'therapeutic': [746], 'relevance': [747], 'treatment': [750], 'particular': [753, 3801], 'congenital': [756], 'muscular': [757], 'dystrophy': [758], '(14Moll': [759], 'Barzaghi': [761], 'P.': [762, 2649, 3917], 'Lin': [763], 'Bezakova': [765], 'Lochmuller': [767], 'Engvall': [769], 'E.': [770, 1374, 1378, 1913, 2647], 'Muller': [771], 'Ruegg': [773, 871, 886, 957, 1037, 1303, 1657, 1746, 1830, 2286, 2511, 4108, 4491, 4528], '413:': [777], '302-307Crossref': [778], '(200)': [781], '16Qiao': [784], 'Li': [786], 'Zhu': [788], 'Draviam': [790], 'R.': [791, 866, 1575, 1596, 1827, 1911, 2653], 'Watkins': [792], 'Ye': [794], 'X.': [795, 799], 'Chen': [796], 'Xiao': [798], '2005;': [807], '102:': [808], '11999-12004Crossref': [809], '(85)': [812, 2542], 'At': [815], 'three': [817, 2470, 3719, 3745], 'within': [819, 1056, 2042, 4413, 4426], 'gene': [822, 844], 'undergo': [823], 'alternative': [824, 1689], 'mRNA': [825, 1690], 'splicing': [826, 1691, 1765], 'resulting': [827], 'several': [829, 1776, 3024], 'protein': [830, 924, 1221, 1488, 1503, 1995, 2239, 2365, 3065, 3216, 3765, 3955, 4023], '(see': [832, 2268, 2380], 'Fig.': [833, 2270, 2382, 4174, 4261, 4451, 4544, 4592], '1A).': [834], 'Alternative': [835, 1214], 'usage': [836, 1215], 'exons': [838, 1205, 1218], '5′-end': [841], 'results': [845, 1219], 'one': [847, 4079, 4091], 'isoform': [848, 909], 'binds': [850, 1723], 'coiled-coil': [853], 'region': [854, 929], 'laminins': [856], 'via': [857, 925], 'N-terminal': [859], '(NtA)': [861], 'domain': [862, 1808, 1862, 1962, 2217, 3971, 3975], '(17Denzer': [863], 'A.J.': [864, 1302, 1652, 2285, 2510, 4107, 4490, 4527], 'Brandenberger': [865], 'Gesemann': [867, 3341], 'Chiquet': [869], '137:': [877], '671-683Crossref': [878], '(138)': [881], '19Mascarenhas': [884], 'J.B.': [885], 'Winzen': [888], 'Halfter': [890], 'Engel': [892, 3343], 'Stetefeld': [894], 'EMBO': [896, 1576, 1597], '22:': [899], '529-536Crossref': [900], '(34)': [903], 'an': [907, 1240, 1943, 2068, 2153, 2976, 3011, 3018, 3212, 3253, 3312, 3572, 3600, 3731, 4248], 'another': [908], 'allows': [911], 'incorporated': [915], 'into': [916, 2399, 2664, 2691, 2712, 2801, 2828, 2888, 2899, 3701, 4549], 'plasma': [917], 'membranes': [918], 'as': [919, 1142, 1541, 2279, 2368, 2547, 2727, 3010, 3334, 3494, 3594, 4009, 4043, 4483], 'type': [921], 'II': [922], 'transmembrane': [923, 927], '(TM)': [928], '(20Burgess': [930, 1010], 'Skarnes': [932, 1012], 'W.C.': [933, 1013], '2000;': [939, 1019], '151:': [940, 1020], '41-52Crossref': [941, 1021], '(142)': [944, 1024], '21Neumann': [947, 1027], 'F.R.': [948, 1028], 'Bittcher': [949, 1029], 'Annies': [951, 1031], 'Schumacher': [953, 1033, 1655, 1744], 'Kroger': [955, 1035], 'Mol.': [959, 1039], '17:': [963, 1043], '208-225Crossref': [964, 1044], '(74)': [967, 1047, 1927], 'The': [970, 1064, 1189, 1237, 1349, 1780, 2635, 2674, 2717, 2790, 3131, 3297, 3390, 3484, 3583, 3697, 3843, 3893, 3966, 3980, 4055, 4204, 4263, 4316], 'secreted,': [971], 'NtA': [972], 'expressed': [977, 1004, 1129, 4042], 'non-neuronal': [979, 1136], 'immobilize': [988], 'synaptic': [992], 'basal': [993], 'lamina': [994], 'while': [998], 'TM': [1000], 'mainly': [1003], 'Two': [1050], 'additional': [1051], 'located': [1055], 'LG2': [1061, 3970, 4075, 4140], 'LG3.': [1063], 'site': [1066, 1090, 2097], 'LG2,': [1068], 'chick': [1072, 1196], 'y': [1074], 'rodents,': [1076, 1200], 'can': [1077], 'contain': [1078], 'four-amino': [1080, 1706], '(A4)': [1084], 'encodes': [1086], 'heparin': [1088], '(22Campanelli': [1091], 'J.T.': [1092, 1280], 'Gayer': [1093], 'G.G.': [1094], 'Development.': [1097], '122:': [1099], '1663-1672Crossref': [1100], '24Hopf': [1104], 'Hoch': [1106, 1277], 'Chem.': [1110, 1750, 1916], '271:': [1112], '5231-5236Abstract': [1113], 'included': [1125, 3891], 'transcripts': [1128], 'glial': [1133], 'whereas': [1135], 'periphery,': [1140], 'such': [1141, 1540], 'muscle,': [1143], 'Schwann,': [1144], 'kidney': [1146], 'cells,': [1147], '(25Ruegg': [1151, 1249], 'Tsim': [1153, 1251], 'K.W.': [1154, 1252], 'Horton': [1155, 1253], 'S.E.': [1156, 1254], 'Kröger': [1157, 1255], 'Escher': [1159, 1257], 'Gensch': [1161, 1259], 'E.M.': [1162, 1260], 'Neuron.': [1165, 1263, 1287, 1335, 1659], '1992;': [1166, 1264, 1288, 1520, 1558], '8:': [1167, 1265, 1289], '691-699Abstract': [1168, 1266], '(224)': [1174, 1272], '27Stone': [1177], 'D.M.': [1178, 1366, 1428], 'Nikolics': [1179], 'K.': [1180, 1422, 1618, 1823, 2531], '1995;': [1183, 1308, 2291, 2516, 3347, 4113, 4496, 4533], '15:': [1184], '6767-6778Crossref': [1185], 'second': [1190, 4132], 'site,': [1192, 4133], 'z': [1198], 'encoded': [1202, 2844], '24': [1207], 'bp': [1208, 1211], '33': [1210], 'length.': [1213], 'with': [1223, 1493, 1726, 2106, 2310, 2334, 2414, 2435, 2455, 2472, 2489, 2494, 2498, 2670, 2682, 2694, 2703, 2715, 2795, 2804, 2840, 2864, 2966, 2995, 3064, 3149, 3172, 3203, 3240, 3249, 3267, 3273, 3316, 3398, 3416, 3441, 3457, 3506, 3536, 3555, 3566, 3571, 3628, 3680, 3709, 3721, 3730, 3747, 3752, 3868, 3960, 4046, 4096, 4296, 4336, 4458, 4467], '0': [1224], '(B0),': [1225], '8': [1226], '(B8),': [1227], '11': [1228], '(B11),': [1229], 'or': [1230, 2954, 4141, 4157, 4293, 4330], '19': [1231], '(B19)': [1232], 'inserts.': [1236], 'presence': [1238], 'essential': [1243], 'AChR': [1245, 2384, 4094, 4178, 4485, 4521], 'vitro': [1248], '28Ferns': [1275], 'Campanelli': [1279], 'Hall': [1283], 'Z.W.': [1284], '1079-1086Abstract': [1290], '(191)': [1296], '29Gesemann': [1299, 4524], 'Denzer': [1301, 1651, 2284, 2509, 4106, 4489, 4526], '128:': [1309, 2292, 2517, 4114, 4497, 4534], '625-636Crossref': [1310, 2293, 2518, 4115, 4498, 4535], '(208)': [1313, 2296, 2521, 4118, 4501, 4538], 'NMJs': [1322], '(30Burgess': [1325], 'Nguyen': [1327, 1876], 'Q.T.': [1328], 'Son': [1329], 'Y.J.': [1330], '1999;': [1336, 1578, 1599, 1885], '23:': [1337], '33-44Abstract': [1338], '(206)': [1346], 'postsynapse-inducing': [1351], 'mediated': [1354], '(31DeChiara': [1361], 'T.M.': [1362, 1430, 4225], 'Bowen': [1363, 1407], 'D.C.': [1364, 1408], 'Valenzuela': [1365, 1427], 'Simmons': [1367], 'M.V.': [1368], 'Poueymirou': [1369], 'W.T.': [1370], 'Thomas': [1371], 'Kinetz': [1373], 'Compton': [1375], 'D.L.': [1376], 'Rojas': [1377], 'Park': [1379], 'J.S.': [1380, 3921], 'DiStefano': [1383, 1425], 'P.S.': [1384, 1426], 'Glass': [1385], 'D.J.': [1386, 1406], 'Burden': [1387, 1423], 'S.J.': [1388, 1424], 'Yancopoulos': [1389, 1431], 'G.D.': [1390, 1432], '501-512Abstract': [1394], '(765)': [1402], '32Glass': [1405], 'Stitt': [1409], 'T.N.': [1410], 'Radziejewski': [1411], 'Bruno': [1413], 'Ryan': [1415], 'T.E.': [1416], 'Gies': [1417], 'D.R.': [1418], 'Shah': [1419], 'Mattsson': [1421], 'DeChiara': [1429], '513-523Abstract': [1436], '(587)': [1444], 'does': [1449, 2121], 'not': [1450, 1462, 1641, 1793, 2038, 2122, 2177, 4358, 4511], 'bind': [1451], 'directly,': [1454], 'suggesting': [1455], 'signaling': [1460], 'but': [1461, 1795, 2046, 2176], 'component': [1465], 'complex.': [1470], 'Another': [1471], 'involves': [1474], 'α-dystroglycan,': [1484], 'peripheral': [1486], 'membrane': [1487, 3698, 3741], 'tightly': [1491], 'associated': [1492], 'transmembranous': [1495], 'β-dystroglycan': [1496], 'arises': [1498], 'common': [1501], 'precursor': [1502], 'posttranslational': [1505], 'cleavage': [1506], '(33Ibraghimov-Beskrovnaya': [1507, 1545], 'O.': [1508, 1546], 'Ervasti': [1509, 1547], 'J.M.': [1510, 1548], 'Leveille': [1511, 1549], 'C.J.': [1512, 1550], 'Slaughter': [1513, 1551], 'C.A.': [1514, 1552], 'Sernett': [1515, 1553], 'S.W.': [1516, 1554], 'Campbell': [1517, 1555, 1617], 'K.P.': [1518, 1556], '355:': [1521, 1559], '696-702Crossref': [1522, 1560], '(1198)': [1525, 1563], 'Binding': [1528, 3500, 3620], 'been': [1533, 2223, 3147, 3170, 3408], 'found': [1534, 2041], 'other': [1536, 1872, 2147], 'domain-containing': [1538], 'laminin-α1': [1542], '-α2': [1544], '35Talts': [1566], 'J.F.': [1567, 1588], 'Andac': [1568, 1589], 'Z.': [1569, 1590], 'Gohring': [1570, 1591, 1904], 'Brancaccio': [1572, 1593, 1653, 1742], 'Timpl': [1574, 1595, 1910, 2652], '18:': [1579, 1600], '863-870Crossref': [1580, 1601], '(398)': [1583, 1604], 'Scholar),': [1585, 1606, 2523, 3648, 4242], 'perlecan': [1586], '(35Talts': [1587], 'neurexins': [1608], '(36Sugita': [1609], 'Saito': [1611], 'Tang': [1613], 'Satz': [1615], 'Sudhof': [1619, 1880], 'T.C.': [1620, 1881], '154:': [1625], '435-445Crossref': [1626], '(375)': [1629], 'Although': [1632], 'initially': [1633], 'postulated,': [1634], 'AChR-aggregating': [1645, 2035], '(23Gesemann': [1647], 'Cavalli': [1649], '16:': [1661], '755-767Abstract': [1662], '(203)': [1670, 2661], 'Several': [1673], 'lines': [1674], 'indicate': [1677], 'regulated': [1687], 'sites.': [1697], 'For': [1698, 2232, 3275, 4145], 'example,': [1699], 'fragment': [1702, 1733, 2687, 2708, 4070, 4084], 'includes': [1704], 'A-site': [1712], '(agrinA4B8)': [1722], 'severalfold': [1727], 'lower': [1728, 4356, 4602], 'affinity': [1729], 'corresponding': [1732], '(agrinA0B0;': [1738], 'Ref.': [1739], '37Gesemann': [1740], '273:': [1752], '600-605Abstract': [1753], '(119)': [1761], 'Thus,': [1764, 2185, 4471], 'A-': [1768, 2172], 'critical': [1773, 2096], 'determinant': [1774], 'functions': [1777, 1789], 'molecular': [1781, 4551], 'details': [1782, 4552], 'different': [1788, 1810, 1956, 1994, 4202], 'structural': [1796, 1984], 'insights': [1797, 4548], 'obtained': [1798], 'x-ray': [1800, 3872], 'crystallography': [1801], 'solution': [1803, 3291, 3392, 3591, 3859], 'NMR': [1804, 3860], 'LG3': [1807, 1961, 4077, 4142], 'variants': [1813, 2143, 4442, 4478], '(38Stetefeld': [1814], 'Alexandrescu': [1816], 'A.T.': [1817, 3909], 'Maciejewski': [1818], 'M.W.': [1819], 'Jenny': [1820], 'Rathgeb-Szabo': [1822], 'Schulthess': [1824, 3339], 'Landwehr': [1826], 'Frank': [1828], 'Kammerer': [1832], 'R.A.': [1833], 'Structure': [1834], '(Camb).': [1835], '2004;': [1836], '12:': [1837], '503-515Abstract': [1838], 'have': [1849], 'following': [1853], 'conclusions:': [1854], '1)': [1855, 3792], 'overall': [1857], 'fold': [1858], 'agrin-LG3': [1861], 'very': [1864, 4379], 'similar': [1865], '(39Rudenko': [1874], 'Chelliah': [1878], 'Y.': [1879, 1903], 'Deisenhofer': [1882], '99:': [1886], '93-101Abstract': [1887], '(117)': [1895], '42Sasaki': [1898], 'Knyazev': [1900], 'Cheburkin': [1902], 'Tisi': [1906], 'D.': [1907], 'Ullrich': [1908], 'Hohenester': [1912], '2002;': [1917], '277:': [1918], '44164-44170Abstract': [1919], 'Scholar);': [1929], '2)': [1930], 'highly': [1939], 'flexible': [1940], 'indicative': [1941], '"induced-fit"': [1944], 'mechanism': [1945], 'docking': [1947], 'putative': [1950], 'receptor;': [1951], '3)': [1952], 'structure': [1954, 2139, 3861, 3896, 3982], 'forms': [1958], 'diverge': [1963], 'already': [1964], 'seven': [1965], 'before': [1968], 'six': [1970], 'after': [1973, 3301, 4272], 'B-site;': [1975], '4)': [1977], 'depletion': [1978, 1999], 'Ca2+': [1980, 1998], 'LG3B8': [1982, 3875], 'changes': [1985], 'same': [1988, 2452, 4006, 4481, 4517], 'residues': [1991, 2011, 4164], 'where': [1992, 2071, 3793, 4564], 'diverge.': [1997], 'flexibility': [2004], 'LG3B0': [2006, 2018, 3863], 'dynamic': [2010], 'those': [2013, 2060], 'whose': [2014], 'orientations': [2015], 'between': [2017, 3036, 4001, 4168], 'LG3B8.': [2020], 'studies': [2022], 'thus': [2023], 'us': [2025, 4194], 'speculate': [2027], 'involved': [2031, 2061], 'only': [2039, 4139], 'B-splice': [2044], 'outside.': [2048], 'now': [2050], 'mutagenesis': [2053, 2822], 'conspicuous': [2055], 'identify': [2059], 'phosphorylation.': [2064, 2158], 'alanines': [2086, 4586], 'active.': [2089], 'Asn-Glu-Ile': [2105], 'strongest': [2108], 'contribution': [2109, 4422], 'Asn': [2111], 'Ile.': [2113], 'Curiously,': [2114], 'mutation': [2115, 2851, 2885], 'single': [2118, 3781], 'alter': [2123], 'potency': [2125, 2155, 4438, 4595], 'all,': [2127], 'although': [2128], 'construct': [2130, 2554, 2676, 4065], 'which': [2132, 2137, 4243], 'Asn1779,': [2133], 'residue': [2135], 'deviate': [2144], 'each': [2146, 3295], 'mutated': [2149, 4161], 'Ala,': [2151], 'increased': [2154, 3961], 'Finally,': [2159, 3739], 'length': [2164], 'sequence': [2179, 2905], 'affects': [2180], 'our': [2186, 2189], 'support': [2188], 'previous': [2190], 'outside': [2195], 'contribute': [2200], 'Antibodies—Polyclonal': [2205], 'antiserum': [2206, 4250], '194T': [2207], 'raised': [2209], 'against': [2210, 3475], 'His-tagged': [2212], 'extracellular': [2216], '(MuSK-ECD)': [2220], 'had': [2222, 3145, 3169, 3407], 'supernatants': [2226], 'transfected': [2228, 2330, 4028], 'HEK293': [2229, 2258, 4029], 'EBNA': [2230, 4030], 'cells.': [2231], 'first': [2234, 4056, 4184], 'injection': [2235], '∼60': [2236], 'μg': [2237], 'complete': [2241], "Freund's": [2242], 'adjuvant': [2243], 'used.': [2245, 3042], 'Specificity': [2246], 'anti-MuSK-ECD': [2248], 'IgG': [2249, 2926, 2981, 3219, 3257, 3577, 3736, 4326, 4370], 'determined': [2251, 2367], 'Western': [2253, 2377, 4257], 'blot': [2254, 2378, 4258], 'analysis': [2255, 2379, 4259], 'MuSK-transfected': [2257], 'staining': [2262], 'cross-sections': [2263], 'mouse': [2265], 'soleus': [2266], 'supplementary': [2269, 2381, 4543], 'S1).': [2271, 4262], 'Culture': [2273], 'Transfections—C2C12': [2275], 'myotubes': [2276, 2400, 2432, 3048, 3185, 4271, 4291], 'prepared': [2278], 'previously': [2280, 3146, 3446], 'described': [2281, 2369, 3335, 3495, 3994, 4010], '(29Gesemann': [2282, 2507, 4104, 4487], 'HEK293-EBNA': [2299], '(Invitrogen)': [2301], "Dulbecco's": [2305, 2409], 'modified': [2306, 2410], "Eagle's": [2307, 2411], 'medium': [2308, 2355, 2407, 2412, 2453, 4026], 'supplemented': [2309, 2413, 2454], '10%': [2311], 'fetal': [2312], 'calf': [2313], 'serum': [2314, 3093, 4343], '(PAA),': [2315], '10': [2316, 2418, 2477, 3357, 3399, 3479], 'mm': [2317, 2419, 2932, 3069, 3072, 3075, 3078, 3081, 3084, 3087, 3107, 3110, 3113, 3116, 3162, 3367, 3428, 3468, 3477, 3480, 3514, 3542, 3545, 3686, 3689, 3758, 3762], 'sodium': [2318, 2420, 2933, 3163, 3515], 'pyruvate,': [2319, 2421], '100': [2320, 2324, 2422, 2426, 2921, 3039, 3150, 3250, 3287, 3507, 4506], 'units/ml': [2321, 2423], 'penicillin,': [2322, 2424], 'μg/ml': [2325, 2427, 2457], 'streptomycin.': [2326, 2428], 'Cells': [2327, 2467], 'transiently': [2329], 'serum-free': [2332], 'conditions': [2333], 'expression': [2337, 2666], 'constructs': [2338, 2503, 2813, 2896, 4045, 4137], 'JetPei': [2341], 'transfection': [2342], 'reagent': [2343], 'according': [2344, 3281, 3787, 3835], "supplier's": [2347], 'description': [2348], '(QBiogene).': [2349], 'After': [2350, 2429, 2445, 2992, 3376, 3413, 3451, 3534], '2-7': [2351], 'days,': [2352, 2431], 'conditioned': [2354], 'collected': [2357], 'concentration': [2360, 2917, 3034, 3802, 3812, 4103, 4518], 'recombinant': [2363, 2436, 2919, 4022], 'below.': [2370, 3496], 'Additionally,': [2371], 'analyzed': [2375], 'S2).': [2383, 4545], 'Aggregation': [2385], 'Assay—C2C12': [2386, 3047], 'myoblasts': [2387, 4294], 'seeded': [2389], 'gelatin-coated,': [2391], '35-mm': [2392], 'tissue': [2393], 'culture': [2394], 'dishes': [2395], '(Falcon)': [2396], 'differentiation': [2398], 'induced': [2402, 4519, 4616], 'changing': [2404], 'proliferation': [2406], '5%': [2415, 3692], 'horse': [2416], 'serum,': [2417], '4-6': [2430], 'incubated': [2434, 2449, 2958, 3187, 3243, 3394, 3440, 3554, 3570, 3704, 3726, 4295], '∼16': [2440], 'h': [2441, 2961, 2988, 3194, 3246, 3679, 3729], '37': [2443, 2465], '°C.': [2444, 2466, 2951, 3130, 3197, 3533], 'washing,': [2446], '1': [2456, 2960, 3080, 3090, 3109, 3112, 3541, 3544, 3685, 3688, 3757, 3761, 4328], 'rhodamine-labeled-α-bungarotoxin': [2458], '(Molecular': [2459], 'Probes)': [2460], '45-60': [2462], 'min': [2463, 2478, 3063, 3127, 3303], 'washed': [2469, 3200, 3266, 3565, 3718, 3744], 'times': [2471, 3202, 3239, 3270, 3456, 3720, 3746], 'PBS': [2473, 3173, 3204, 3224, 3537, 3681, 3753], 'fixed': [2475], '2%': [2480], 'PFA.': [2481], 'Fixed': [2482], 'mounted': [2485], 'glass': [2487], 'coverslips': [2488], 'Cityfluor': [2490], '(Plano)': [2491], 'examined': [2493], 'microscope': [2496], 'equipped': [2497], 'epifluorescence': [2499], '(Leica).': [2500], 'Expression': [2501], 'Constructs—The': [2502], 'pc95A4B8,': [2504], 'pc95A0B0,': [2505], 'pc95A4B11': [2506], 'phumanγ1': [2525], '(43Traunecker': [2526], 'Oliveri': [2528], 'Karjalainen': [2530], 'Biotechnol.': [2533], '1991;': [2534], '9:': [2535], '109-113Abstract': [2536], 'PCR': [2548, 2636, 2728, 2791, 2858], 'templates': [2549], 'cDNA': [2553], 'encoding': [2555, 4066, 4138], 'C45A4/0B0/8/11,': [2556], 'LG3B0/8,': [2557], 'LG2A0/4': [2559, 2718, 2735], 'fused': [2560], 'humanγ1-Fc': [2563, 2907], 'tag.': [2564], 'Primers': [2565, 2730, 2761], 'amplify': [2568, 2733], 'fragments': [2570, 4040], 'C45A4B8': [2572, 2681, 2713, 2724, 2802, 2824, 3031, 4459], 'C45A0B0': [2574, 2692, 2702, 2726], 's4655_NheI': [2576], 'CGA': [2577, 2738], 'AGC': [2578, 2581, 2615, 2739, 2742, 2769, 2778], 'TCA': [2579, 2596, 2740], 'GTG': [2580, 2597, 2741], 'AGG': [2582, 2632, 2743], 'ACC': [2583, 2744], 'ACA': [2584, 2745], 'CCA': [2585, 2616, 2746], 'TG': [2586, 2747], 'as6227_BamHI': [2588], 'CAC': [2589, 2750, 2782], 'GAG': [2590, 2633, 2783], 'GAT': [2591, 2752, 2757, 2773, 2784], 'CCC': [2592, 2629, 2753, 2785], 'TTT': [2593, 2786], 'GGC': [2594, 2777, 2787], 'TGA': [2595, 2611, 2770, 2775, 2788], 'TAA': [2598], 'TA': [2599], 'primers': [2601, 2839], 'amplification': [2605], 'humanγ1-Fc-tag': [2608], 'shγ1_BamHI': [2610], 'GGA': [2612, 2630, 2756], 'TCC': [2613], 'TAG': [2614], 'AAT': [2617], 'CTT': [2618], 'CTG': [2619], 'AC': [2620], 'ashγ1_XhoI': [2622], 'GCT': [2623, 2768], 'GAC': [2624, 2631], 'TCG': [2625], 'AGT': [2626, 2755, 2772], 'CAT': [2627, 2774], 'TTA': [2628], 'AG.': [2634], 'products': [2637, 2792], 'cloned': [2639, 2827], 'in-frame': [2640], 'BM-40': [2643, 2903], 'signal': [2644, 2904, 4286, 4305, 4318, 4354], 'peptide': [2645], '(44Kohfeldt': [2646], 'Maurer': [2648], 'Vannahme': [2650], 'FEBS': [2654, 3345], 'Lett.': [2655, 3346], '414:': [2657], '557-561Crossref': [2658], 'vector': [2667], 'pCEP-Pu': [2668, 2900], 'cut': [2669, 2803, 3700], 'XhoI': [2671], 'NheI.': [2673], 'C45A4B0': [2675, 4468, 4509], 'generated': [2678, 2699, 2722, 2819, 3977, 4136], 'digesting': [2680, 2701], 'HindIII/XhoI': [2683], 'ligating': [2685, 2706], 'containing': [2688, 2709, 2883, 2901, 3174, 3205, 3225, 3538, 3682, 3754, 3871], 'A4-insert': [2690], 'opened': [2693, 2714], 'HindIII/XhoI.': [2695], 'Similarly,': [2696, 4340], 'C45A0B8': [2697], 'BamHI/XhoI': [2704], 'B0-insert': [2711], 'BamHI/XhoI.': [2716], 'LG3B0/8': [2720, 2764], 'template.': [2729], 's4655_NheI,': [2737], 'as_cAgrin-BamHI_G2': [2749], 'CAG': [2751], 'GAA': [2754, 2771, 2776], 'TTC': [2758], 'AAC': [2759], 'GG.': [2760], 's_G3_NheI': [2766], 'ATA': [2767], 'TGG,': [2779], 'as_cAgrin_BamHI_G3': [2781], 'GC.': [2789], 'digested': [2794], 'NheI': [2796], 'BamHI': [2798], 'ligated': [2800], 'NheI/BamHI.': [2805], 'Sequencing': [2806], 'assure': [2810], 'correct.': [2815], 'Mutant': [2816], 'pBluescript': [2829], 'KS+': [2830], '(Stratagene).': [2831], 'Mutations': [2832, 2890], 'introduced': [2834], 'forward': [2836], 'reverse': [2838], 'overlapping': [2841], '5′-ends': [2842], 'sequences': [2845, 2853], 'either': [2847], 'side': [2848], '(primer': [2852], 'available': [2855], 'request).': [2857], 'whole': [2862], 'plasmid': [2863, 2882], 'expand': [2866], 'polymerase': [2867], 'kit': [2868], '(Roche': [2869], 'Applied': [2870], 'Science).': [2871], 'Template': [2872], 'DNA': [2873], 'removed': [2875], 'DpnI': [2877], 'digestion': [2878], 'new': [2881], 'transformed': [2887], 'DH10B.': [2889], 'confirmed': [2892], 'sequencing.': [2894], 'Mutated': [2895], 'subcloned': [2898], 'tag': [2908], 'BamHI/EcoRI': [2910], 'digestion.': [2911], 'Quantification': [2912], 'Agrin—To': [2914], 'determine': [2915, 4196, 4420], 'agrin,': [2920], 'μl': [2922, 3151, 3251, 3288], 'goat': [2924, 2978, 3574, 3733], 'anti-humanγ1': [2925], '(5': [2927, 3510], 'μg/ml,': [2928, 3158], 'BIOSOURCE)': [2929, 2985], '50': [2931, 3161, 3366, 3427, 3513], 'carbonate': [2934, 3164, 3516], 'buffer': [2935, 3105, 3411, 3417, 3449, 3458, 3517, 3568, 3723, 3749], '(pH': [2936], '9.6)': [2937], 'immobilized': [2939, 3520], '96-well': [2942, 3053, 3138, 3523], 'microtiter': [2945, 3141, 3190, 3526], 'plate': [2946, 3142, 3264, 3527], '(Costar)': [2947, 3143], 'overnight': [2948, 3213, 3530, 3705], '4': [2950, 3129, 3196, 3238, 3245, 3396, 3532, 3707], 'Agrin-containing': [2952], 'media': [2953], 'followed': [2962, 3210, 4460], '3': [2964, 2987], 'washes': [2965, 2994, 3415], 'PBST': [2967, 3241, 3268], '(PBS': [2968], '+': [2969, 4282, 4302], '0.05%': [2970, 3539, 3683, 3755], 'Tween': [2971, 3207, 3227], '20)': [2972], 'incubation': [2974, 3214], 'HRP-conjugated': [2977, 3254, 3573, 3732], 'anti-human': [2979, 3575, 3734], 'γ1': [2980, 3576, 3735, 4052], 'antibody': [2982, 3155, 4216], '(70': [2983, 3578], 'ng/ml;': [2984], 'room': [2990], 'temperature.': [2991], 'four': [2993, 3201, 3269], 'PBST,': [2996], 'amount': [2998, 3584], 'measured': [3002, 3016, 3310, 3588, 3598, 3798], 'McEvans': [3005, 3590], 'solution,': [3006], 'ABTS': [3007], 'H2O2': [3009], 'HRP': [3012, 3586], 'substrate.': [3013, 3595], 'Absorbance': [3014, 3596], 'ELISA-reader': [3019], '405': [3021, 3604], 'nm': [3022, 3322, 4276, 4298, 4507], 'time': [3025], 'points.': [3026], 'As': [3027, 4375, 4448, 4589], 'standard,': [3029], 'affinity-purified': [3030], 'range': [3035], '0.1': [3037], 'ng/ml': [3040, 3579], 'Solid': [3043, 3497], 'Phase': [3044, 3498], 'Phosphorylation': [3046], 'grown': [3050], 'gelatin-coated': [3052], 'plates': [3054, 3191, 4335], '(Falcon).': [3055], 'stimulations': [3057], 'routinely': [3059], 'done': [3060, 3333], '30': [3062, 3126, 3302], 'diluted': [3066, 3558, 3711], '0.44': [3068], 'KH2PO4,': [3070], '1.34': [3071], 'Na2HPO4,': [3073], '5.6': [3074], 'glucose,': [3076], '137': [3077], 'NaCl,': [3079, 3117, 3478], 'MgCl2,': [3082], '1.25': [3083], 'CaCl2,': [3085, 3543, 3687], '25': [3086], 'HEPES,': [3088, 3108], 'mg/ml': [3091], 'bovine': [3092], 'albumin,': [3094], 'pH': [3095, 3166, 3369, 3430, 3482, 3518], '7.4.': [3096, 3483], 'Myotubes': [3097], 'subsequently': [3099, 3725], 'rinsed': [3100, 3237], 'extracted': [3102, 3132], 'lysis': [3104], '(20': [3106, 3963], 'EDTA,': [3111], 'EGTA,': [3114], '150': [3115, 3476], '1%': [3118], 'Nonidet': [3119], 'P40,': [3120], 'phosphatase,': [3121], 'protease': [3123], 'inhibitors)': [3124], 'transferred': [3135, 3650], 'coated': [3148], 'anti-phosphotyrosine': [3154], '4G10': [3156, 4217, 4337], '(1': [3157, 3737], 'Upstate)': [3159], 'buffer,': [3165, 3632], '9.6': [3167], 'blocked': [3171, 3676], '0.5%': [3175, 3178], 'Tween-20': [3176], 'Top': [3179, 3231], 'Block': [3180, 3232], '(Juro).': [3181], 'Extracts': [3182], 'C2C12': [3184, 4270, 4407], '5': [3193, 3442, 3455, 3616], 'Plates': [3198, 3235], '0.1%': [3206, 3226, 3230], '20': [3208, 3228], '(PBST)': [3209], 'A-purified': [3217], 'anti-MuSK': [3218, 4249, 4325, 4349], '(194T;': [3220], 'dilution': [3221], '1:5,000)': [3222], '(incubation': [3233], 'buffer).': [3234, 3582, 3696], '4°C': [3248], 'donkey': [3255], 'anti-rabbit': [3256], '(0.2': [3258], 'μg/ml;': [3259], 'Jackson': [3260], 'Immunoresearch).': [3261], 'Subsequently,': [3262], 'once': [3272, 3751], 'PBS.': [3274], 'detection,': [3276], 'QuantaBlue': [3277, 3305], '(Pierce)': [3278], 'mixed': [3280], "manufacturer's": [3284], 'advice,': [3285], 'added': [3293, 4313], 'well.': [3296], 'reaction': [3298], 'stopped': [3300], 'stop': [3306], 'solution.': [3307], 'Fluorescence': [3308], 'ELISA': [3313, 3601, 4264], 'reader': [3314, 3602], '(Gemini)': [3315], 'excitation': [3318], 'wavelength': [3319], '325': [3321], 'detection': [3324, 3772], '420': [3326], 'nm.': [3327, 3605], 'Purification': [3328], 'α-Dystroglycan—Purification': [3330], 'essentially': [3332], 'elsewhere': [3336], '(45Brancaccio': [3337], '368:': [3348], '139-142Crossref': [3349], '(137)': [3352], 'In': [3355, 4608], 'brief,': [3356], 'g': [3358], 'frozen': [3360], 'chicken': [3361, 3622], 'breast': [3362], 'homogenized': [3364], 'Tris-HCl,': [3368, 3429, 3481], '7.4;': [3370, 3431], '0.2': [3371], 'm': [3372, 3433], 'NaCl': [3373, 3434], '(buffer': [3374, 3435], 'A).': [3375], 'centrifugation': [3377], '(12,000': [3378], '×': [3379], 'g),': [3380], 'supernatant': [3382], 'filtered': [3384], 'through': [3385], 'Whatman': [3387], 'filter': [3388], 'paper.': [3389], 'cleared': [3391], '°C': [3397, 3708], 'ml': [3400, 3443], 'DEAESephacel': [3402], 'beads': [3403, 3425], '(Amersham': [3404], 'Biosciences)': [3405], 'equilibrated': [3409, 3447], 'extensive': [3414], 'A,': [3418], 'bound': [3419], 'eluted': [3422], '0.5': [3432], 'B).': [3436], 'Eluates': [3437], 'then': [3439, 3473, 3564], 'WGA-Sepharose': [3445], 'washing': [3452], 'resin': [3454], 'elution': [3460], 'WGASepharose': [3462], 'achieved': [3464], 'adding': [3466], '300': [3467], 'N-acetyglucosamine.': [3469], 'Eluted': [3470], 'dialyzed': [3474], 'enrichment': [3485], 'verified': [3489], 'transfer': [3491], 'overlay': [3492], 'assay': [3493, 4190, 4205, 4393], 'α-Dystroglycan': [3499], 'Assay—Enzyme-linked': [3501], 'assays': [3503], 'performed': [3505, 3614], 'μlof': [3508], 'μg/ml)': [3511], '9.6,': [3519], 'absorption': [3529], 'blocking': [3535, 3560, 3567, 3581, 3713, 3722, 3748], 'Tween-20,': [3540, 3684, 3756], 'MgCl2': [3546, 3690], '3%': [3548], 'BSA': [3549], '(blocking': [3550, 3695], 'buffer),': [3551], 'wells': [3552], 'buffer.': [3561, 3714], 'Wells': [3562], 'ABTS/H2O2': [3593], 'To': [3606, 3997, 4121, 4546], 'avoid': [3607, 3998], 'saturation': [3608, 3824], 'signal,': [3611, 4391], 'measurements': [3612], 'every': [3615], 'min.': [3617], 'Transfer': [3618], 'Overlay': [3619], 'Assay—Purified': [3621], 'denatured': [3627], 'reducing': [3629], 'SDS': [3630], 'sample': [3631], 'separated': [3633], '3-12%': [3636], 'SDS-PAGE': [3637], '(46Laemmli': [3638], 'U.K.': [3639], '1970;': [3641], '227:': [3642], '680-685Crossref': [3643], '(207538)': [3646], 'nitrocellulose': [3652], '(47Towbin': [3653], 'Staehelin': [3655], 'Gordon': [3657], '1979;': [3666], '76:': [3667], '4350-4354Crossref': [3668], '(44939)': [3671], 'Blots': [3674], '2': [3678, 3728, 4275, 4297], 'milk': [3693], 'powder': [3694], 'strips': [3702, 3716, 3742], 'Nitrocellulose': [3715], 'μg/ml).': [3738], 'CaCl2': [3759], 'MgCl2.': [3763], 'Immunoreactive': [3764], 'bands': [3766], 'visualized': [3768], 'ECL': [3771], 'method': [3773], '(Pierce).': [3774], 'Curve': [3775], 'Fitting—Data': [3776], 'fitted': [3778], 'class': [3782], 'equivalent': [3784], 'Equation': [3789], '1,': [3790], 'Ai=Asat×[c/(Kd+c)+A0](Eq.': [3791], 'Ai': [3794], 'represents': [3795], 'absorbance': [3797], 'ligand,': [3805, 3815, 3830], 'Kd': [3806], 'dissociation': [3808], 'constant,': [3809], 'c': [3810], 'Asat': [3817], 'A0': [3819], 'absorbances': [3822], 'absence': [3828], 'respectively.': [3831], 'normalized': [3834], 'equation': [3837], '(Ai': [3838], '-': [3839, 3841], 'A0)/(Asat': [3840], 'A0).': [3842], 'software': [3844], 'Prism': [3847], 'GraphPad': [3848], '4.0.': [3849], 'Modeling—The': [3850], 'model': [3853, 3967], 'built': [3855], '(PDB': [3864, 3876, 3881], 'code:': [3865, 3877, 3882], '1Q56)': [3866], 'together': [3867], 'calcium': [3870], '1PZ8)': [3878], 'LG3B11': [3880], '1PZ9).': [3883], 'Residues': [3884], 'missing': [3885], 'manually.': [3892], 'lowest': [3894, 4457], 'energy': [3895], 'subject': [3898], '200': [3900], 'cycles': [3901], 'unrestrained': [3903], 'Powell': [3904], 'minimization': [3905], 'CNS': [3907], '(48Brunger': [3908], 'Adams': [3910], 'P.D.': [3911], 'Clore': [3912], 'G.M.': [3913], 'DeLano': [3914], 'W.L.': [3915], 'Gros': [3916], 'Grosse-Kunstleve': [3918], 'Jiang': [3920], 'Kuszewski': [3922], 'Nilges': [3924], 'Pannu': [3926], 'N.S.': [3927], 'Read': [3928], 'R.J.': [3929], 'Rice': [3930], 'L.M.': [3931], 'Simonson': [3932], 'Warren': [3934], 'G.L.': [3935], 'Acta': [3936], 'Crystallogr.': [3937, 3940], 'D': [3938], '54:': [3942], '905-921Crossref': [3943], '(16979)': [3946], 'Harmonic': [3949], 'restraints': [3950], 'imposed': [3952], 'atoms': [3956], '(3': [3957], 'kcal/mol': [3958, 3964], 'Å2)': [3959], 'weight': [3962], 'Å2).': [3965], 'EGF': [3974], 'SWISS-MODEL.': [3979], 'tandem': [3981], 'LG2-EGF-LG3': [3984], 'created': [3986], 'basis': [3989], 'typical': [3991], 'elbow': [3992], 'angle': [3993], 'Fv-fragments.': [3996], 'sterical': [3999], 'clashes': [4000], 'LG2-EGF': [4002], 'EGF-LG3,': [4004], 'refinement': [4007], 'protocol': [4008], 'above': [4011], 'performed.': [4013], 'Constructs': [4014], 'Cellular': [4016], 'Assays—To': [4017], 'facilitate': [4018], 'purification': [4019], 'increase': [4034], 'apparent': [4035], 'affinities,': [4037], 'fusion': [4044], 'Fc': [4048], 'part': [4049], 'human': [4051], '(Fig.': [4053, 4143, 4206, 4279, 4576], '1B).': [4054, 4144], 'set': [4057], 'mutants': [4060, 4563], 'derived': [4062], '45-kDa,': [4068], '(called': [4071], 'C45A0/4B0/8/11)': [4072], 'comprises': [4074], 'EGF-like': [4080], '(EG)': [4081], 'domain.': [4082], 'smallest': [4090], 'half-maximal': [4098], 'response': [4099], '(EC50)': [4100], 'picomolar': [4102], 'test': [4122], 'influence': [4124], 'individual': [4126], 'independent': [4129], 'competition': [4146], 'experiments,': [4147], 'further': [4149], 'agrinA0B0': [4155], '(N25C95A0B0': [4156], 'mini-agrin;': [4158], '14).': [4159], 'identical': [4167], 'fish': [4169], 'mammals': [4171], '(bold': [4172], '1C).': [4175], 'Because': [4176], 'agrin-induced': [4177, 4382, 4554], 'phosphorylation,': [4182, 4515, 4556], 'established': [4185], 'two-side': [4187], 'enzyme-linked': [4188], 'immunosorbent': [4189], '(ELISA)': [4191], 'allowed': [4193], 'reliable': [4197], 'dose-response': [4198], 'curves': [4199], 'constructs.': [4203], '1D)': [4207], 'use': [4212], 'monoclonal': [4215], '(49Cohen': [4218], 'Yoakim': [4220], 'Piwnica-Worms': [4222], 'Roberts': [4224], 'Schaffhausen': [4226], 'B.S.': [4227], '87:': [4236], '4458-4462Crossref': [4237], '(65)': [4240], 'recognizes': [4244, 4252], 'tyrosine-phosphorylated': [4245, 4369], 'immunohistochemistry': [4255], '(supplementary': [4260], 'detects': [4265], 'stimulation': [4273], 'neural': [4277, 4299], '1E;': [4280], 'MT': [4281], 'agrin).': [4283, 4303], 'nonspecific': [4285], 'detected': [4288, 4466], 'non-treated': [4290], '(MT)': [4292], '(MB': [4301], 'persisted': [4306], 'when': [4308, 4341], 'no': [4309], 'cell': [4310], 'extract': [4311], '(w/o': [4314, 4327, 4338], 'cells).': [4315], 'background': [4317, 4353, 4366, 4377], 'was,': [4319], 'however,': [4320, 4613], 'lost': [4321], 'upon': [4322], 'omitting': [4323], 'Ab)': [4329], 'coating': [4332], 'coating).': [4339], 'preimmune': [4342], 'instead': [4346], 'antiserum,': [4350], 'became': [4355], '(data': [4357], 'shown).': [4359], 'indicates': [4361], 'relatively': [4364], 'originates': [4367], 'primary': [4373], 'antiserum.': [4374], 'stable': [4380], 'resulted': [4385], 'consistent,': [4388], 'sensitive': [4396], 'reproducible': [4398], 'enough': [4399], 'measure': [4401], 'myotubes.': [4408], 'Contribution': [4409], 'Amino': [4411], 'Acids': [4412], 'Agrin-induced': [4417], 'Phosphorylation—To': [4419], 'B-inserts': [4428], 'activity,': [4434], 'compared': [4436], 'assay.': [4447], 'shown': [4449, 4590], '2A,': [4452], 'EC50': [4454], 'value': [4455], 'C45A4B11.': [4462], 'No': [4463], 'C45A0B0.': [4470], 'follows': [4479], 'order': [4482], 'Whereas': [4504], 'did': [4510], 'some': [4520], 'clusters': [4522], '(Ref.': [4523], 'Scholar;': [4540], 'get': [4547], 'next': [4558], 'made': [4559], 'series': [4561], 'B8': [4570], 'altered': [4573], 'alanine': [4575], '2B).': [4577], 'First,': [4578], '(mutant': [4587], 'C45B8-8A).': [4588], '2C,': [4593], 'efficacy': [4597], 'C45B8-8A': [4599, 4614], 'much': [4601], 'wild-type': [4606], 'C45A4B8.': [4607], 'contrast': [4610], 'C45A4B0,': [4612], 'phosp': [4618]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2070438439', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 3}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 2}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2025-01-11T08:14:41.257716', 'created_date': '2016-06-24'}
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