Novel Monoclonal Antibodies Demonstrate Biochemical Variation of Brain Parkin with Age

Name: Work Video:
Duration: 3 min 30 s
Aaron C. Pawlyk; Benoit I. Giasson; Deepak M. Sampathu; Francisco A. Perez; Kah‐Leong Lim; Valina L. Dawson; Ted M. Dawson; Richard D. Palmiter; John Q. Trojanowski; Virginia M.‐Y. Lee
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'https://openalex.org/W2120886143', 'https://openalex.org/W2123056519', 'https://openalex.org/W2132591384', 'https://openalex.org/W2136000143', 'https://openalex.org/W2142780460', 'https://openalex.org/W2168909179', 'https://openalex.org/W2169363048', 'https://openalex.org/W2314185656'], 'related_works': ['https://openalex.org/W4248431072', 'https://openalex.org/W3049065873', 'https://openalex.org/W2884144427', 'https://openalex.org/W2383678931', 'https://openalex.org/W2359931691', 'https://openalex.org/W2178039548', 'https://openalex.org/W2067317860', 'https://openalex.org/W2035149159', 'https://openalex.org/W1974898145', 'https://openalex.org/W154169386'], 'abstract_inverted_index': {'Autosomal': [0, 221, 442], 'recessive': [1, 218, 222, 439, 443, 452, 1046], 'juvenile': [2, 37, 219, 223, 258, 440, 444, 453, 1047], 'parkinsonism': [3, 224, 445, 489, 1048], 'is': [4, 30, 102, 115, 131, 162, 225, 251, 323, 336, 352, 383, 483, 550, 1549, 1635, 4409], 'a': [5, 91, 226, 312, 551, 556, 569, 573, 720, 1636, 1938, 1953, 1992, 2638, 2760, 3072, 3139, 3188, 3896, 4182, 4209, 4371, 4434], 'movement': [6, 213, 227, 434, 1906], 'disorder': [7, 228], 'associated': [8, 229], 'with': [9, 230, 457, 555, 1053, 1567, 1849, 1875, 1892, 1941, 2023, 2067, 2123, 2184, 2213, 2458, 2546, 2750, 2821, 2944, 3176, 3272, 3298, 3320, 3343, 3362, 3487, 3499, 3523, 3645, 3878, 3893, 3919, 3936, 4057, 4066, 4098, 4131, 4152, 4159, 4166, 4171, 4174, 4229, 4291, 4308, 4364], 'the': [10, 31, 86, 106, 119, 137, 146, 156, 174, 208, 231, 252, 307, 327, 340, 358, 367, 377, 395, 429, 496, 513, 628, 697, 706, 731, 745, 908, 934, 1570, 1642, 1657, 1834, 1862, 1901, 1919, 2028, 2189, 2228, 2256, 2615, 2619, 2804, 2810, 2814, 2875, 2896, 2917, 2936, 2988, 3038, 3046, 3094, 3132, 3177, 3230, 3252, 3258, 3307, 3381, 3393, 3404, 3658, 3907, 4020, 4064, 4132, 4146, 4233, 4240, 4265, 4302, 4402, 4410], 'degeneration': [11, 232], 'of': [12, 22, 36, 60, 76, 90, 93, 110, 158, 177, 210, 233, 243, 257, 281, 297, 311, 314, 331, 379, 398, 431, 488, 499, 560, 578, 585, 615, 627, 648, 699, 708, 733, 747, 910, 1572, 1646, 1660, 1795, 1808, 1817, 1833, 1837, 1851, 1859, 1903, 2027, 2036, 2061, 2121, 2188, 2210, 2219, 2262, 2518, 2584, 2607, 2610, 2614, 2710, 2774, 2823, 2833, 2842, 2853, 2884, 2905, 3040, 3141, 3147, 3169, 3212, 3380, 3390, 3395, 3403, 3475, 3504, 3511, 3898, 4019, 4029, 4211, 4242, 4270, 4273, 4298, 4352, 4401, 4436, 4456], 'dopaminergic': [13, 234], 'neurons': [14, 235], 'in': [15, 45, 105, 117, 126, 136, 145, 155, 164, 168, 173, 198, 201, 212, 236, 266, 326, 338, 347, 357, 366, 376, 385, 389, 394, 419, 422, 433, 621, 632, 639, 728, 750, 1580, 1656, 1824, 1839, 1861, 1873, 1886, 1890, 1905, 1926, 1952, 1997, 2491, 2515, 2541, 2566, 2630, 2652, 2692, 2713, 2759, 2771, 2908, 2951, 2997, 3008, 3053, 3103, 3288, 3292, 3350, 3392, 3408, 3521, 3889, 4033, 4060, 4070, 4112, 4310, 4330, 4336, 4346, 4360, 4390, 4439, 4447], 'substantia': [16, 237], 'nigra': [17, 238], 'pars': [18, 239], 'compacta.': [19, 240], 'The': [20, 241, 1911, 1930, 1987, 2019, 2091, 2186, 2259, 2632, 2677, 2739, 2931, 3283, 3326, 3337, 3397, 3501, 3727, 3987, 4418], 'loss': [21, 242], 'functional': [23, 244], 'parkin': [24, 27, 61, 71, 101, 159, 197, 211, 245, 248, 282, 292, 322, 380, 418, 432, 520, 549, 640, 739, 918, 1548, 1650, 1661, 1803, 1818, 1838, 1860, 1871, 1885, 1904, 1914, 1933, 2115, 2126, 2296, 2487, 2648, 3358, 3641, 3894, 4021, 4032, 4139, 4219, 4244, 4299, 4313, 4323, 4403, 4429], 'caused': [25, 246], 'by': [26, 183, 247, 404, 925, 1562, 1845, 1866, 2032, 2119, 2157, 2176, 2194, 2200, 2267, 2273, 2276, 2369, 2373, 2522, 2589, 2604, 2850, 2995, 3269, 3317, 3366, 3516, 3759, 3916, 4036, 4063, 4184, 4236, 4399, 4414], 'gene': [28, 249, 514], 'mutations': [29, 250, 631, 638, 899], 'most': [32, 253, 629, 2613], 'common': [33, 254, 630], 'single': [34, 255], 'cause': [35, 256], 'parkinsonism.': [38, 220, 259, 441], 'Parkin': [39, 130, 260, 351, 1575], 'has': [40, 261, 713, 740, 1576], 'been': [41, 73, 262, 294, 643, 714, 741, 904, 1578], 'shown': [42, 263, 644, 715, 4345], 'to': [43, 56, 195, 264, 277, 416, 509, 645, 743, 901, 906, 1802, 1883, 1991, 2168, 2281, 2303, 2394, 2562, 2576, 2595, 2725, 2895, 3004, 3078, 3116, 3210, 3229, 3244, 3311, 3377, 3387, 3755, 3882, 3884, 4025, 4091, 4145, 4207, 4287, 4296], 'aid': [44, 265], 'protecting': [46, 267], 'cells': [47, 268, 921, 1553, 1928, 1949, 2167, 2212, 2480, 2483, 2512, 2538, 4042, 4109, 4168], 'from': [48, 149, 269, 370, 922, 1551, 1557, 1584, 2100, 2161, 2681, 2803, 2942, 3037, 3264, 3478, 3482, 4254, 4264, 4312, 4338, 4450], 'endoplasmic': [49, 270], 'reticulum': [50, 271], 'and': [51, 88, 122, 141, 272, 309, 343, 362, 512, 518, 572, 613, 711, 724, 736, 758, 1559, 1644, 1799, 1821, 1826, 1841, 1877, 1924, 1970, 1974, 2053, 2056, 2085, 2096, 2106, 2165, 2253, 2291, 2297, 2300, 2466, 2481, 2504, 2510, 2533, 2555, 2574, 2597, 2625, 2647, 2659, 2699, 2718, 2735, 2790, 2813, 2818, 2835, 2877, 2882, 2919, 2946, 2969, 2972, 2991, 3024, 3066, 3160, 3218, 3274, 3278, 3315, 3328, 3356, 3400, 3497, 3507, 3651, 3667, 3900, 3940, 4017, 4031, 4052, 4078, 4094, 4121, 4157, 4190, 4257, 4283, 4318, 4327, 4354, 4370, 4378, 4394], 'oxidative': [52, 273], 'stressors': [53, 274], 'presumably': [54, 275], 'due': [55, 276], 'ubiquitin': [57, 278, 935], 'ligase': [58, 279, 723], 'activity': [59, 280], 'that': [62, 100, 283, 321, 490, 563, 725, 917, 1547, 1813, 2684, 4297, 4443], 'targets': [63, 284], 'proteins': [64, 285, 930, 2198, 4309], 'for': [65, 80, 286, 301, 504, 931, 1981, 2231, 2295, 2579, 2618, 2673, 2687, 2703, 2796, 2925, 2978, 3030, 3074, 3084, 3122, 3237, 3323, 3369, 3951, 4054, 4072, 4232], 'proteasomal': [66, 287], 'degradation.': [67, 288], 'However,': [68, 289, 1633], 'studies': [69, 290, 702, 1094, 1899, 2654], 'on': [70, 291, 582, 704, 1641, 1937, 2289, 2637, 2916, 3071, 4045], 'have': [72, 293, 641, 903, 1545], 'impeded': [74, 295], 'because': [75, 296], 'limited': [77, 298], 'reagents': [78, 299, 4142], 'specific': [79, 187, 300, 408, 1800, 1870, 4206], 'this': [81, 302, 505, 709, 2714, 3409, 4406, 4431], 'protein.': [82, 303], 'Here': [83, 304], 'we': [84, 305, 1880, 4246], 'report': [85, 306], 'generation': [87, 308], 'characterization': [89, 310], 'panel': [92, 313, 1807, 4272], 'parkin-specific': [94, 315], 'monoclonal': [95, 189, 316, 410, 1069, 1810, 3730], 'antibodies.': [96, 317], 'Biochemical': [97, 318], 'analyses': [98, 319, 1832, 2689, 3950, 4250], 'indicate': [99, 320], 'present': [103, 116, 132, 324, 337, 353, 4446], 'only': [104, 325, 4276], 'high': [107, 120, 138, 328, 341, 359, 1059, 1062, 2775], 'salt-extractable': [108, 121, 139, 329, 342, 360], 'fraction': [109, 125, 140, 148, 330, 346, 361, 369], 'mouse': [111, 332, 1840, 2669, 3636, 3729, 4331, 4396, 4440], 'brain,': [112, 333], 'whereas': [113, 334], 'it': [114, 191, 335, 412, 712, 716], 'both': [118, 339, 4391], 'RIPA-resistant,': [123, 344], 'SDS-extractable': [124, 147, 345, 368], 'young': [127, 348], 'human': [128, 151, 180, 349, 372, 401, 1558, 1585, 1647, 1827, 1842, 1863, 1913, 2125, 2486, 2682, 2766, 3265, 3890, 4218, 4266, 4361], 'brain.': [129, 152, 350, 373, 1864, 4267], 'at': [133, 142, 354, 363, 1977, 1984, 2731, 2792, 2799, 2921, 2928, 2974, 2981, 3026, 3033, 3068, 3080, 3087, 3118, 3125, 3240, 3303, 3334, 4186, 4301, 4385], 'decreased': [134, 355], 'levels': [135, 144, 356, 365], 'increased': [143, 364], 'aged': [150, 371, 2660, 2719], 'This': [153, 374], 'shift': [154, 375], 'extractability': [157, 378], 'upon': [160, 381], 'aging': [161, 382], 'seen': [163, 384], 'humans': [165, 386], 'but': [166, 387, 634, 4334, 4405], 'not': [167, 193, 388, 414, 3385, 4335, 4426], 'mice,': [169, 390], 'demonstrating': [170, 391], 'species-specific': [171, 392], 'differences': [172, 393], 'biochemical': [175, 396, 1835, 1876, 2674, 2688], 'characteristics': [176, 397], 'murine': [178, 399, 1825, 4212], 'versus': [179, 400, 1648], 'parkin.': [181, 402, 2185, 2220], 'Finally,': [182, 403, 1865], 'using': [184, 405, 1563, 1846, 1867, 2158, 2181, 2197, 2277, 2374, 2839, 2887, 2902, 3166, 3183, 3251, 3657, 4140, 4251], 'these': [185, 406, 748, 911, 1639, 1796, 1868, 2223, 2653, 2704, 3041, 4243], 'highly': [186, 407, 1869], 'anti-parkin': [188, 409, 2190, 4278, 4422, 4458], 'antibodies,': [190, 411, 3895], 'was': [192, 413, 507, 515, 1819, 1829, 1916, 1935, 1989, 2021, 2065, 2155, 2265, 2367, 2892, 2914, 2933, 3174, 3208, 3227, 3276, 3286, 3309, 3359, 3375, 3519, 3654, 3753, 4198, 4214, 4358, 4388], 'possible': [194, 415, 4027], 'detect': [196, 417, 1884, 3756, 4092, 4288], 'α-synuclein-containing': [199, 420], 'lesions': [200, 421, 1889], 'α-synucleinopathies,': [202, 423], 'thereby': [203, 424, 1894], 'challenging': [204, 425], 'prior': [205, 426, 1898, 2594, 2894, 3077, 3115, 3243], 'inferences': [206, 427], 'about': [207, 428, 1900], 'role': [209, 430, 1902], 'disorders': [214, 435, 1907], 'other': [215, 436, 1908, 4419], 'than': [216, 437, 1909], 'autosomal': [217, 438, 1045], '(AR-JP)': [446], '1The': [447], 'abbreviations': [448, 2740], 'used': [449, 2651, 2686, 2702, 2712, 3407, 3754, 4024, 4090], 'are:': [450], 'AR-JPautosomal': [451], 'parkinsonismELISAenzyme-linked': [454], 'immunosorbent': [455, 1050, 2178], 'assayDLBdementia': [456], 'Lewy': [458, 1054, 1581, 2751], 'bodiesGCIglial': [459], 'cytoplasmic': [460, 1057], 'inclusionHShigh': [461], 'salt': [462, 464, 467, 1060, 1063, 1067, 1565, 2776, 2955], 'bufferHSThigh': [463], 'Triton': [465, 1064, 2846, 3011, 3018, 3348, 4068], 'bufferLSlow': [466], 'buffermAbmonoclonal': [468], 'antibodyMSAmultiple': [469], 'system': [470, 1072, 2755, 3662], 'atrophyPBSphosphate-buffered': [471], "salinePDParkinson's": [472], 'diseasePMIpost-mortem': [473], 'intervalPMSFphenylmethanesulfonyl': [474], 'fluorideRIPAradioactive': [475], 'immunoprecipitation': [476, 1083], 'assay': [477, 1051, 1084, 2179, 2592, 3180], 'bufferIBRin-between': [478], 'RING': [479, 580, 588, 1087, 2241, 2248], 'fingerCHOChinese': [480], 'hamster': [481, 1090, 2477], 'ovaryDAPI4′,6-diamidino-2-phenylindole.': [482], 'an': [484, 565, 586, 3378], 'especially': [485], 'insidious': [486], 'form': [487], 'can': [491, 717], 'strike': [492], 'as': [493, 495, 719, 752, 754, 1554, 1556, 1652, 1654, 2127, 2309, 2397, 2706, 2742, 2809, 2874, 2935, 2987, 3045, 3093, 3131, 3187, 3280, 3413, 3669, 3761, 3956, 4049, 4200, 4220, 4260, 4262], 'early': [494], '1st': [497], 'decade': [498], 'life.': [500], 'A': [501, 1806, 2899, 4349, 4380], 'major': [502, 4411], 'locus': [503], 'disease': [506, 1077], 'mapped': [508, 2193], 'chromosome': [510], '6q,': [511], 'subsequently': [516], 'identified': [517, 4226], 'termed': [519], '(1Kitada': [521], 'T.': [522, 665, 768, 770, 945, 947, 1294, 1433, 1475, 1600, 1711, 1747, 1779, 3542], 'Asakawa': [523, 1301, 1478, 1718, 1750], 'S.': [524, 532, 655, 766, 860, 943, 1022, 1157, 1296, 1302, 1304, 1471, 1479, 1481, 1523, 1713, 1719, 1721, 1743, 1751, 1753, 2138, 2317, 2349, 2405, 2437, 3527, 3610, 3825, 3857, 3964, 4001], 'Hattori': [525, 794, 995, 1019, 1099, 1309, 1400, 1438, 1468, 1605, 1726, 1740], 'N.': [526, 538, 663, 675, 795, 996, 1020, 1100, 1172, 1306, 1310, 1346, 1401, 1429, 1439, 1469, 1485, 1596, 1606, 1723, 1727, 1741, 1757, 3531], 'Matsumine': [527, 1476, 1748], 'H.': [528, 791, 850, 879, 994, 1096, 1131, 1298, 1334, 1348, 1399, 1435, 1467, 1477, 1602, 1715, 1739, 1749, 1773, 3540, 3779], 'Yamamura': [529, 1482, 1754], 'Y.': [530, 536, 762, 803, 844, 873, 877, 939, 968, 990, 998, 1024, 1108, 1125, 1129, 1182, 1300, 1308, 1373, 1395, 1403, 1443, 1483, 1487, 1529, 1610, 1717, 1725, 1755, 1759, 1777, 3536], 'Minoshima': [531, 1303, 1480, 1720, 1752], 'Yokochi': [533], 'M.': [534, 653, 683, 764, 941, 970, 992, 1018, 1153, 1271, 1324, 1350, 1375, 1397, 1427, 1473, 1499, 1594, 1745, 2134, 2345, 2433, 3435, 3690, 3853, 3997], 'Mizuno': [535, 802, 997, 1023, 1107, 1307, 1402, 1442, 1486, 1609, 1724, 1758], 'Shimizu': [537, 1305, 1484, 1722, 1756, 1778], 'Nature.': [539], '1998;': [540], '392:': [541], '605-608Crossref': [542], 'PubMed': [543, 607, 691, 785, 812, 838, 865, 893, 962, 984, 1010, 1040, 1117, 1145, 1164, 1194, 1217, 1242, 1263, 1286, 1316, 1367, 1389, 1415, 1461, 1493, 1540, 1628, 1682, 1703, 1733, 1765, 1787, 2149, 2335, 2360, 2423, 2448, 3447, 3570, 3630, 3702, 3748, 3795, 3815, 3843, 3868, 3982, 4012], 'Scopus': [544, 608, 692, 786, 813, 839, 866, 894, 963, 985, 1011, 1041, 1118, 1146, 1165, 1195, 1218, 1243, 1264, 1287, 1317, 1368, 1390, 1416, 1462, 1494, 1541, 1629, 1683, 1704, 1734, 1766, 1788, 2150, 2336, 2361, 2424, 2449, 3448, 3571, 3631, 3703, 3749, 3796, 3816, 3844, 3869, 3983, 4013], '(4323)': [545], 'Google': [546, 610, 694, 788, 815, 841, 868, 896, 965, 987, 1013, 1043, 1120, 1148, 1167, 1197, 1220, 1245, 1266, 1289, 1319, 1341, 1370, 1392, 1418, 1464, 1496, 1543, 1631, 1685, 1706, 1736, 1768, 1790, 2152, 2338, 2363, 2426, 2451, 3450, 3470, 3573, 3633, 3705, 3725, 3751, 3798, 3818, 3846, 3871, 3985, 4015], 'Scholar).': [547, 611, 695, 897, 1044, 1632, 1791, 2153, 2364, 2452, 3471, 3634, 3726, 3872, 3986], 'Human': [548], '465-amino': [552], 'acid': [553, 1943, 1994, 2591], 'protein': [554, 1853, 1934, 2454, 2825, 3172, 3179, 3391, 4235, 4289, 4437], 'predicted': [557, 2260], 'molecular': [558, 4293], 'mass': [559], '52': [561], 'kDa': [562, 4387], 'contains': [564], 'N-terminal': [566], 'ubiquitin-like': [567, 2620, 4460], 'domain,': [568, 2235], 'linker': [570, 2237, 4463], 'region,': [571, 2238, 2245], 'C-terminal': [574], 'TRIAD': [575], 'domain': [576, 2233], 'consisting': [577], 'two': [579, 2831, 3005, 3270], 'fingers': [581], 'either': [583, 2375], 'side': [584], 'in-between': [587, 1086, 4466], '(IBR)': [589], 'finger': [590, 1088], 'region': [591, 2230, 2617], '(2van': [592], 'der': [593], 'Reijden': [594], 'B.A.': [595], 'Erpelinck-Verschueren': [596], 'C.A.': [597, 883, 1135], 'Lowenberg': [598], 'B.': [599, 824, 1026, 1362, 1511, 1525], 'Jansen': [600, 3613], 'J.H.': [601], 'Protein': [602, 2582], 'Sci.': [603, 858, 3561], '1999;': [604, 1490, 1762, 3562], '8:': [605], '1557-1561Crossref': [606], '(64)': [609, 1369], 'Deletions': [612], 'insertions': [614], 'one': [616], 'or': [617, 928, 1568, 2384, 2765, 3480, 3493, 3640, 3911, 3923, 3928, 3938, 4195], 'more': [618], 'exons': [619], 'resulting': [620, 1931, 3284], 'premature': [622, 923], 'translation': [623], 'termination': [624], 'are': [625, 2690, 2722, 2741, 3513, 4344], 'some': [626, 1794, 4400], 'parkin,': [633, 700, 2611, 4208], 'numerous': [635], 'missense': [636], 'point': [637], 'also': [642, 1577, 3875, 4357, 4445], 'be': [646], 'causal': [647], 'AR-JP': [649, 902], '(3West': [650], 'A.': [651, 673, 677, 681, 799, 828, 861, 1104, 1155, 1176, 1188, 1509, 1513, 1515, 1531, 1771, 2623, 3461, 3533, 3606, 3716], 'Periquet': [652], 'Lincoln': [654], 'Lucking': [656], 'C.B.': [657], 'Nicholl': [658], 'D.': [659, 671, 1273, 1356, 2627, 3425, 3554, 3578, 3612, 3680], 'Bonifati': [660], 'V.': [661, 1151, 1503], 'Rawal': [662], 'Gasser': [664], 'Lohmann': [666], 'E.': [667, 826, 1358, 3552], 'Deleuze': [668], 'J.F.': [669, 818], 'Maraganore': [670], 'Levey': [672], 'Wood': [674], 'Durr': [676], 'Hardy': [678, 1353], 'J.': [679, 685, 846, 881, 973, 1029, 1133, 1159, 1189, 1202, 1227, 1236, 1311, 1336, 1354, 1378, 1451, 1517, 1534, 1618, 1667, 1676, 1728, 2143, 2325, 2354, 2413, 2442, 3440, 3455, 3465, 3559, 3590, 3695, 3710, 3720, 3785, 3833, 3862, 3972, 4006], 'Brice': [680, 1187, 1530], 'Farrer': [682], 'Am.': [684, 1450, 1617, 2324, 2412, 3464, 3719, 3784, 3832, 3971], 'Med.': [686, 889, 1141], 'Genet.': [687], '2002;': [688, 777, 954, 1032, 1453, 1620, 1784, 2327, 2415, 3745, 3812, 3835, 3974], '114:': [689], '584-591Crossref': [690], '(192)': [693], 'Since': [696], 'identification': [698], 'many': [701], 'focused': [703], 'elucidating': [705], 'function': [707, 718], 'protein,': [710, 3170], 'ubiquitin-protein': [721], 'isopeptide': [722], 'its': [726], 'overexpression': [727], 'vivo': [729], 'enhances': [730], 'ubiquitinylation': [732], 'synphilin-1,': [734], 'Pael-R,': [735], 'CDCRel-1.': [737], 'Immunoprecipitated': [738], 'reported': [742, 905, 1546], 'catalyze': [744], 'ubiquination': [746, 909], 'substrates': [749], 'vitro': [751], 'well': [753, 1555, 1653, 4261], 'O-glycosylated': [755], 'α-synuclein': [756, 1887, 3517, 3757, 3886, 4030, 4034], 'p22': [757], 'cyclin': [759], 'E': [760], '(4Imai': [761, 938], 'Soda': [763, 940, 969, 991, 1374, 1396], 'Hatakeyama': [765, 942], 'Akagi': [767, 944], 'Hashikawa': [769, 946], 'Nakayama': [771, 948], 'K.I.': [772, 949], 'Takahashi': [773, 950, 971, 999, 1376, 1404], 'R.': [774, 801, 951, 972, 1000, 1106, 1186, 1377, 1405, 1437, 1527, 1604, 2132, 2343, 2431, 2626, 3851, 3995], 'Mol.': [775, 952], 'Cell.': [776, 953, 1001, 1406], '10:': [778, 955], '55-67Abstract': [779, 956], 'Full': [780, 782, 833, 835, 957, 959, 979, 981, 1005, 1007, 1035, 1037, 1384, 1386, 1410, 1412, 1456, 1458, 1623, 1625, 2330, 2332, 2418, 2420, 3565, 3567, 3790, 3792, 3838, 3840, 3977, 3979], 'Text': [781, 783, 834, 836, 958, 960, 980, 982, 1006, 1008, 1036, 1038, 1385, 1387, 1411, 1413, 1457, 1459, 1624, 1626, 2331, 2333, 2419, 2421, 3566, 3568, 3791, 3793, 3839, 3841, 3978, 3980], 'PDF': [784, 837, 961, 983, 1009, 1039, 1388, 1414, 1460, 1627, 2334, 2422, 3569, 3794, 3842, 3981], '(435)': [787, 964], 'Scholar,': [789, 816, 842, 869, 966, 988, 1014, 1121, 1149, 1168, 1198, 1221, 1246, 1267, 1290, 1320, 1342, 1371, 1393, 1419, 1465, 1497, 1686, 1707, 1737, 1769, 2339, 2427, 3451, 3574, 3706, 3799, 3819, 3847], '5Shimura': [790], 'Schlossmacher': [792, 1097], 'M.G.': [793, 1098, 1421, 1588], 'Frosch': [796, 1101, 1422, 1589], 'M.P.': [797, 1102, 1423, 1507, 1590], 'Trockenbacher': [798, 1103], 'Schneider': [800, 1105], 'Kosik': [804, 1109, 1448, 1615], 'K.S.': [805, 1110, 1449, 1616], 'Selkoe': [806, 1111, 1446, 1613], 'D.J.': [807, 1112, 1447, 1614], 'Science.': [808, 1113], '2001;': [809, 890, 1002, 1114, 1142, 1214, 1239, 1283, 1313, 1364, 1407, 1537, 1679, 1730], '293:': [810, 1115], '263-269Crossref': [811, 1116], '(973)': [814, 1119], '6Staropoli': [817], 'McDermott': [819], 'C.': [820, 822, 1332, 1505, 3604, 3616], 'Martinat': [821], 'Schulman': [823], 'Demireva': [825], 'Abeliovich': [827], 'Neuron.': [829], '2003;': [830], '37:': [831], '735-749Abstract': [832], '(351)': [840], '7Zhang': [843], 'Gao': [845, 880, 1132], 'Chung': [847], 'K.K.': [848, 871, 1123], 'Huang': [849, 878, 1130], 'Dawson': [851, 853, 884, 886, 1136, 1138], 'V.L.': [852, 885, 1137], 'T.M.': [854, 887, 1139], 'Proc.': [855], 'Natl.': [856], 'Acad.': [857], 'U.': [859], '2000;': [862, 976, 1161, 1191, 1260, 1338, 1381, 1700, 2146, 2357, 2445, 3444, 3699, 3787, 3865, 4009], '97:': [863], '13354-13359Crossref': [864], '(849)': [867], '8Chung': [870, 1122], 'Zhang': [872, 1124], 'Lim': [874, 1126], 'K.L.': [875, 1127], 'Tanaka': [876, 1128], 'Ross': [882, 1134], 'Nat.': [888, 1140], '7:': [891, 1143], '1144-1150Crossref': [892, 1144], '(676)': [895, 1147], 'Furthermore,': [898], 'linked': [900], 'reduce': [907], 'substrates.': [912], 'Moreover,': [913], 'emerging': [914], 'data': [915], 'suggest': [916], 'may': [919], 'protect': [920], 'death': [924, 2732], 'targeting': [926], 'misfolded': [927], 'damaged': [929], 'degradation': [932], 'via': [933], 'proteasome': [936], 'pathway': [937], '18Imai': [967, 1372], 'Biol.': [974, 1030, 1379], 'Chem.': [975, 1031, 1237, 1380, 1677], '275:': [977, 1382], '35661-35664Abstract': [978, 1383], '(663)': [986, 1391], '19Imai': [989, 1394], 'Inoue': [993, 1398], '105:': [1003, 1408], '891-902Abstract': [1004, 1409], '(942)': [1012, 1417], '38Hyun': [1015], 'D.H.': [1016], 'Lee': [1017, 1359, 2141, 2320, 2352, 2408, 2440, 3436, 3456, 3691, 3711, 3739, 3780, 3806, 3828, 3860, 3967, 4004], 'Kubo': [1021, 1470, 1742], 'Halliwell': [1025], 'Jenner': [1027], 'P.': [1028, 1330, 1344], '277:': [1033], '28572-72857Abstract': [1034], '(163)': [1042], 'enzyme-linked': [1049, 2177], 'dementia': [1052, 2749], 'bodies': [1055], 'glial': [1056], 'inclusion': [1058], 'buffer': [1061, 1065, 1068, 1085, 1954, 1999, 2030, 2038, 2778, 2957, 3014, 3057, 3097, 3107, 3192, 3221, 3224, 3374], 'low': [1066, 1564, 2954], 'antibody': [1070, 3731, 3990, 4423, 4432], 'multiple': [1071, 2754], 'atrophy': [1073], 'phosphate-buffered': [1074], 'saline': [1075], "Parkinson's": [1076], 'post-mortem': [1078, 2736], 'interval': [1079, 2737], 'phenylmethanesulfonyl': [1080], 'fluoride': [1081], 'radioactive': [1082], 'Chinese': [1089, 2476], 'ovary': [1091, 2478], '4′,6-diamidino-2-phenylindole.': [1092], 'Numerous': [1093], '(5Shimura': [1095], "9D'Agata": [1150], 'Grimaldi': [1152], 'Pascale': [1154], 'Cavallaro': [1156], 'Eur.': [1158, 1335], 'Neurosci.': [1160, 1337, 2144, 2355, 2443, 3863, 4007], '12:': [1162, 1339, 1365], '3583-3588Crossref': [1163], '(31)': [1166], '10Gu': [1169], 'W.J.': [1170, 1501], 'Abbas': [1171], 'Lagunes': [1173], 'M.Z.': [1174], 'Parent': [1175, 1512], 'Pradier': [1177, 1520], 'L.': [1178, 1352, 1431, 1521, 1598], 'Bohme': [1179], 'G.A.': [1180, 1519], 'Agid': [1181, 1528], 'Hirsch': [1183, 1532], 'E.C.': [1184, 1533], 'Raisman-Vozari': [1185, 1526], 'Neurochem.': [1190, 1312, 1729], '74:': [1192], '1773-1776Crossref': [1193], '(44)': [1196], '11Horowitz': [1199], 'J.M.': [1200, 1223, 1360, 1663], 'Myers': [1201, 1226, 1666], 'Vernace': [1203, 1224, 1664], 'V.A.': [1204, 1225, 1665], 'Stachowiak': [1205, 1228, 1668], 'M.K.': [1206, 1229, 1669], 'Torres': [1207, 1234, 1674], 'G.': [1208, 1235, 1675, 3596], 'Brain': [1209, 1212, 1278, 1281, 1782], 'Res.': [1210, 1213, 1279, 1282, 1783, 2145, 2356, 2444, 3864, 4008], 'Dev.': [1211, 1280], '126:': [1215], '31-41Crossref': [1216], '(17)': [1219], '12Horowitz': [1222], 'Hanlon': [1230, 1670], 'D.W.': [1231, 1671, 3584], 'Fraley': [1232, 1672], 'G.S.': [1233, 1673], 'Neuroanat.': [1238, 1678], '21:': [1240, 1680], '75-93Crossref': [1241, 1681], '(38)': [1244, 1288, 1684], '13Huynh': [1247, 1687], 'D.P.': [1248, 1269, 1688, 2319, 2407, 3588, 3827, 3966], 'Scoles': [1249, 1689], 'D.R.': [1250, 1690], 'Ho': [1251, 1691], 'T.H.': [1252, 1692], 'Del': [1253, 1693], 'Bigio': [1254, 1694], 'M.R.': [1255, 1695], 'Pulst': [1256, 1276, 1696], 'S.M.': [1257, 1277, 1697, 3433, 3688, 3777], 'Ann.': [1258, 1488, 1698, 1760, 3743, 3810], 'Neurol.': [1259, 1489, 1536, 1699, 1761, 3443, 3560, 3698, 3744, 3811], '48:': [1261, 1701], '737-744Crossref': [1262, 1702], '(69)': [1265, 1705], '14Huynh': [1268], 'Dy': [1270], 'Nguyen': [1272], 'Kiehl': [1274], 'T.R.': [1275], '130:': [1284], '173-181Crossref': [1285], '15Kubo': [1291, 1708], 'S.I.': [1292, 1709], 'Kitami': [1293, 1710], 'Noda': [1295, 1712], 'Shimura': [1297, 1434, 1601, 1714], 'Uchiyama': [1299, 1716], '78:': [1314, 1731], '42-54Crossref': [1315, 1732], '(117)': [1318, 1735], '16Stichel': [1321], 'C.C.': [1322], 'Augustin': [1323], 'Kuhn': [1325], 'K.': [1326, 1775, 1781, 3580], 'Zhu': [1327], 'X.R.': [1328], 'Engels': [1329], 'Ullmer': [1331], 'Lubbert': [1333], '4181-4194PubMed': [1340], '17Choi': [1343], 'Golts': [1345], 'Snyder': [1347], 'Chong': [1349], 'Petrucelli': [1351], 'Sparkman': [1355], 'Cochran': [1357], 'Wolozin': [1361], 'Neuroreport.': [1363], '2839-2843Crossref': [1366], '20Schlossmacher': [1420], 'Gai': [1424, 1591], 'W.P.': [1425, 1592], 'Medina': [1426, 1593], 'Sharma': [1428, 1595], 'Forno': [1430, 1597], 'Ochiishi': [1432, 1599], 'Sharon': [1436, 1603], 'Langston': [1440, 1607], 'J.W.': [1441, 1608], 'Hyman': [1444, 1611], 'B.T.': [1445, 1612], 'Pathol.': [1452, 1619, 2326, 2414, 3466, 3721, 3786, 3834, 3973], '160:': [1454, 1621, 2328, 2416, 3836, 3975], '1655-1667Abstract': [1455, 1622], '(278)': [1463, 1630], '21Shimura': [1466, 1738], 'Yoshikawa': [1472, 1744], 'Kitada': [1474, 1746], '45:': [1491, 1763], '668-672Crossref': [1492, 1764], '(259)': [1495, 1767], '22Zarate-Lagunes': [1498], 'Gu': [1500], 'Blanchard': [1502], 'Francois': [1504], 'Muriel': [1506], 'Mouatt-Prigent': [1508], 'Bonici': [1510], 'Hartmann': [1514], 'Yelnik': [1516], 'Boehme': [1518], 'Moussaoui': [1522], 'Faucheux': [1524], 'Comp.': [1535], '432:': [1538], '184-196Crossref': [1539], '(47)': [1542], 'Scholar)': [1544, 3752, 4016], 'extractable': [1550], 'cultured': [1552], 'rodent': [1560, 1649], 'brain': [1561, 1586, 1828, 1843, 2678, 3952, 4252, 4332, 4362, 4397, 4441, 4448], 'buffers': [1566, 1850, 2822, 3137], 'without': [1569, 3225], 'addition': [1571], 'mild': [1573], 'detergents.': [1574], 'found': [1579], 'body-enriched': [1582], 'preparations': [1583], '(20Schlossmacher': [1587], 'there': [1634], 'discrepancy': [1637], 'among': [1638], 'reports': [1640], 'number': [1643, 4435], 'size': [1645], 'isoforms': [1651], 'discrepancies': [1655], 'subcellular': [1658], 'distribution': [1659], '(12Horowitz': [1662], '23Hase': [1770], 'Yamada': [1772], 'Arai': [1774], 'Sunada': [1776], 'Matsumura': [1780], '930:': [1785], '143-149Crossref': [1786], '(12)': [1789], 'To': [1792, 2221, 4203, 4238], 'clarify': [1793], 'issues,': [1797], 'sensitive': [1798], 'antibodies': [1801, 1811, 2094, 2280, 2399, 4084, 4205, 4279], 'were': [1804, 1881, 1950, 2098, 2117, 2174, 2192, 2251, 2271, 2287, 2456, 2489, 2513, 2539, 2560, 2587, 2602, 2635, 2655, 2664, 2671, 2685, 2701, 2769, 2807, 2816, 2837, 2872, 2949, 2985, 2993, 3002, 3043, 3050, 3091, 3101, 3129, 3235, 3249, 3262, 3332, 3339, 3384, 3411, 3485, 3643, 3874, 3903, 3954, 4023, 4043, 4089, 4096, 4110, 4129, 4155, 4169, 4285, 4444], 'generated.': [1805], 'antiparkin': [1809], '(mAb)': [1812], 'recognize': [1814, 4427], 'different': [1815], 'domains': [1816, 2218], 'produced,': [1820], 'their': [1822, 2726], 'specificity': [1823, 4241], 'demonstrated.': [1830], 'Detailed': [1831], 'properties': [1836, 1858], 'tissue': [1844, 2679, 2767, 3268, 3405, 3473, 3914, 4039], 'serial': [1847], 'fractionation': [1848, 2675, 2806], 'increasing': [1852, 2824], 'extraction': [1854, 2696, 2826, 2834, 2901, 3096, 3136, 3220], 'strength': [1855], 'showed': [1856], 'unusual': [1857], 'mAbs': [1872, 2116, 2191, 4022, 4213, 4324], 'conjunction': [1874], 'immunostaining': [1878], 'methods,': [1879], 'unable': [1882], 'containing': [1888, 1955], 'patients': [1891, 2943, 2948], 'α-synucleinopathies': [1893, 2945, 3514], 'calling': [1895], 'into': [1896, 1918, 2255, 4181], 'question': [1897], 'AR-JP.': [1910], 'full-length': [1912, 4137], 'cDNA': [1915], 'cloned': [1917], 'baculovirus': [1920], 'expression': [1921], 'vector': [1922], 'pFASTBAC-HTb': [1923], 'expressed': [1925, 2199], 'Sf9': [1927], '(Invitrogen).': [1929, 1945, 4163], 'His6-tagged': [1932], 'purified': [1936], 'column': [1939, 1995, 2020, 2025, 2034, 2059, 3254], 'packed': [1940], 'nickel-nitrilotriacetic': [1942, 1993], 'resin': [1944], 'In': [1946, 3880], 'brief,': [1947], 'Sf9/parkin': [1948], 'lysed': [1951, 2565], '50': [1956, 2569, 2866, 2909, 3110, 3293, 3351], 'mm': [1957, 1965, 1968, 2001, 2009, 2012, 2015, 2040, 2048, 2051, 2069, 2077, 2080, 2083, 2570, 2780, 2785, 2788, 2856, 2867, 2910, 2959, 2964, 2967, 3111, 3162, 3194, 3199, 3202, 3294, 3352, 3491, 3649, 4178], 'Tris-HCl,': [1958, 2002, 2041, 2070], 'pH': [1959, 2003, 2042, 2071, 2572, 2782, 2869, 2912, 2961, 3113, 3196, 3296, 3354], '8.5,': [1960, 2004, 2043, 2072], '0.1': [1961, 2073], 'm': [1962, 2006, 2045, 2074, 3022, 3064, 3290, 3313, 3345], 'KCl,': [1963, 2007, 2046, 2075], '5': [1964, 2011, 2047, 2079, 2580, 2963, 3238], '2-mercaptoethanol,': [1966, 2013, 2049, 2081], '1': [1967, 2014, 2044, 2050, 2082, 2966, 3075, 3198, 3370, 4317], 'PMSF,': [1969, 2016, 2052, 2084], '1%': [1971, 2845, 2863, 3017, 3060, 3204, 3347], 'Nonidet': [1972, 2864], 'P-40': [1973], 'then': [1975, 2057, 3051, 3360], 'centrifuged': [1976], '10,000': [1978], '×': [1979, 2640, 2794, 2923, 2976, 3028, 3082, 3120, 3305, 4188], 'g': [1980, 2795, 2924, 2977, 3029, 3083, 3121], '15': [1982], 'min': [1983, 2798, 2927, 2980, 3032, 3086, 3124, 3239, 4056], '4': [1985, 2800, 2982, 3034, 3324, 3335], '°C.': [1986, 2801, 2930, 2983, 3035, 3089, 3127, 3336], 'supernatant': [1988, 2932, 3308], 'applied': [1990], 'pre-equilibrated': [1996], 'equilibration': [1998, 2062], '(20': [2000, 2039], '0.5': [2005, 3021, 3063, 3161], '20': [2008, 2068], 'imidazole,': [2010, 2078], '10%': [2017, 2054, 2086, 2496, 2526, 2548, 2970, 3206, 4116], 'glycerol).': [2018], 'washed': [2022, 2817, 2994, 3340, 4170], '10': [2024, 2290, 2952, 3009, 3054, 4055, 4073], 'volumes': [2026, 2035, 2060], 'same': [2029, 4303], 'followed': [2031, 2849, 3915, 4062], '2': [2033, 2058, 2202, 2609, 2772, 2784, 2840, 2851, 2903, 3312, 3344], 'wash': [2037], 'glycerol)': [2055], 'buffer.': [2063, 2999], 'His6-parkin': [2064], 'eluted': [2066, 3361], '100': [2076, 2501, 2505, 2530, 2534, 2552, 2556, 2577, 3241, 4122, 4125], 'glycerol': [2087], '(see': [2088], 'Fig.': [2089, 4347], '1A).': [2090], 'rabbit': [2092, 3988], 'polyclonal': [2093], 'CS2132': [2095, 4375, 4415], 'AB5112': [2097, 4424], 'purchased': [2099, 4421], 'Cell': [2101], 'Signaling': [2102], 'Technology': [2103], '(Beverly,': [2104], 'MA)': [2105], 'Chemicon': [2107], 'International,': [2108], 'Inc.': [2109], '(Temecula,': [2110], 'CA),': [2111], 'respectively.': [2112, 2880], 'Murine': [2113], 'anti-human': [2114], 'generated': [2118, 2603, 3250], 'immunization': [2120], 'mice': [2122, 2164, 2601, 2634, 2650, 2663, 4259, 4315, 4452], 'recombinant': [2124, 4217, 4234], 'described': [2128, 2310, 2723, 3281, 3414, 3670, 3762, 3957, 4050, 4201, 4221], '(30Giasson': [2129, 3992], 'B.I.': [2130, 2341, 2429, 3419, 3674, 3736, 3767, 3803, 3849, 3993], 'Jakes': [2131, 2342, 2430, 3850, 3994], 'Goedert': [2133, 2344, 2432, 3852, 3996], 'Duda': [2135, 2346, 2434, 3854, 3998], 'J.E.': [2136, 2347, 2435, 3417, 3672, 3734, 3765, 3801, 3855, 3999], 'Leight': [2137, 2348, 2436, 3856, 4000], 'Trojanowski': [2139, 2322, 2350, 2410, 2438, 3438, 3462, 3693, 3717, 3741, 3782, 3808, 3830, 3858, 3969, 4002], 'J.Q.': [2140, 2323, 2351, 2411, 2439, 3439, 3463, 3694, 3718, 3742, 3783, 3809, 3831, 3859, 3970, 4003], 'V.M.-Y.': [2142, 2321, 2353, 2409, 2441, 3437, 3457, 3692, 3712, 3740, 3781, 3807, 3829, 3861, 3968, 4005], '59:': [2147, 2358, 2446, 3445, 3700, 3866, 4010], '528-533Crossref': [2148, 2359, 2447, 3867, 4011], '(194)': [2151, 2362, 2450, 3870, 4014], 'Fusion': [2154], 'conducted': [2156, 2368, 2838, 2915, 3412, 4247], 'spleen': [2159], 'lymphocytes': [2160], 'immunized': [2162], 'Balb/c': [2163], 'SP2': [2166], 'produce': [2169, 4204], 'hybridomas.': [2170], 'Resulting': [2171, 3000], 'hybridoma': [2172], 'supernatants': [2173], 'screened': [2175], '(ELISA)': [2180], 'plates': [2182], 'coated': [2183], 'epitopes': [2187], 'Western': [2195, 2365, 2598, 4248, 4342], 'blotting': [2196, 2366], 'Gene-Porter': [2201], '(Gene': [2203], 'Therapy': [2204], 'Systems,': [2205], 'San': [2206], 'Diego,': [2207], 'CA)-mediated': [2208], 'transfection': [2209], 'QBI293': [2211], 'pcDNA3.1-myc/His': [2214], '(Invitrogen)': [2215, 2545, 4143], 'harboring': [2216], 'subcloned': [2217], 'generate': [2222], 'constructs,': [2224], 'PCR': [2225], 'fragments': [2226], 'spanning': [2227], 'coding': [2229, 2616], 'each': [2232, 2263, 2282, 2829, 3146], '(ubiquitin-like': [2234], '1–77;': [2236], '78–220;': [2239], 'first': [2240, 3039], 'finger,': [2242, 2249], '221–305;': [2243], 'IBR': [2244], '306–398;': [2246], 'second': [2247, 4470], '399–465)': [2250], 'digested': [2252], 'ligated': [2254], 'host': [2257], 'vector.': [2258], 'sequence': [2261], 'construct': [2264], 'confirmed': [2266, 2728], 'DNA': [2268], 'sequencing.': [2269], 'Antibodies': [2270], 'isotyped': [2272], 'antigen-captured': [2274], 'ELISA': [2275], 'goat': [2278, 2376, 2385], 'anti-mouse': [2279, 2377], 'immunoglobulin': [2283], 'subtype': [2284], '(Sigma).': [2285], 'Proteins': [2286, 3261], 'resolved': [2288], '15%': [2292], 'SDS-polyacrylamide': [2293], 'gels': [2294], 'α-synuclein,': [2298], 'respectively,': [2299], 'electrophoretically': [2301], 'transferred': [2302], 'nitrocellulose': [2304], 'membranes': [2305], '(Schleicher': [2306], '&': [2307], 'Schuell)': [2308], 'previously': [2311, 3415, 3763, 3958], '(29Forman': [2312, 2400, 3959], 'M.S.': [2313, 2401, 3821, 3960], 'Schmidt': [2314, 2402, 3822, 3961], 'M.L.': [2315, 2403, 3453, 3708, 3823, 3962], 'Kasturi': [2316, 2404, 3824, 3963], 'Perl': [2318, 2406, 3826, 3965], '1725-1731Abstract': [2329, 2417, 3837, 3976], '(114)': [2337, 2425, 3845, 3984], '30Giasson': [2340, 2428, 3848], 'following': [2370, 3257], 'published': [2371, 3524], 'protocols': [2372], '(Jackson': [2378], 'Immuno-Research': [2379], 'Laboratories,': [2380, 3664, 4105], 'West': [2381], 'Grove,': [2382], 'PA)': [2383], 'anti-rabbit': [2386], '(Santa': [2387], 'Cruz': [2388], 'Biotechnology,': [2389], 'Santa': [2390], 'Cruz,': [2391], 'CA)': [2392, 3666], 'conjugated': [2393], 'horseradish': [2395], 'peroxidase': [2396], 'secondary': [2398, 4083], 'Immuno-positive': [2453], 'bands': [2455, 4290, 4329, 4438], 'detected': [2457, 4325, 4359, 4389, 4433], 'Renaissance': [2459], 'Enhanced': [2460], 'Luminol': [2461], 'Reagents': [2462], '(PerkinElmer': [2463], 'Life': [2464], 'Sciences)': [2465], 'X-Omat': [2467], 'Blue': [2468], 'XB-1': [2469], 'film': [2470], '(Eastman': [2471], 'Kodak': [2472], 'Co.).': [2473], 'Wild': [2474], 'type': [2475, 2646, 2662, 3639, 4256, 4393], '(CHO)': [2479], 'CHO': [2482, 4108], 'stably': [2484, 4150], 'expressing': [2485, 4136], '(CHO-PAR4)': [2488], 'maintained': [2490, 2514, 2540, 2636, 4111, 4158], 'α-minimal': [2492, 4113], 'essential': [2493, 4114], 'medium': [2494, 2520, 2543, 4103], '(Invitrogen),': [2495, 2500, 4120], 'fetal': [2497, 2527, 2549, 4117], 'bovine': [2498, 2528, 2550, 3184, 4118], 'serum': [2499, 3185, 4119], 'units/ml': [2502, 2506, 2531, 2535, 2553, 2557, 4123, 4126], 'penicillin,': [2503, 2532, 2554, 4124], 'streptomycin.': [2507, 2536, 2558, 4127], 'HeLa,': [2508], 'Neuro-2A,': [2509], 'HEK-293': [2511], "Dulbecco's": [2516], 'modification': [2517], "Eagle's": [2519], '(Cellgro': [2521], 'Mediatech,': [2523], 'Herndon,': [2524], 'VA),': [2525], 'serum,': [2529, 2551], 'SH-SY5Y': [2537], 'RPMI': [2542], '1640': [2544], 'l-glutamine,': [2547], 'Cells': [2559, 4128, 4149], 'grown': [2561, 4044], '∼80%': [2563], 'confluency,': [2564], '2%': [2567, 2906], 'SDS,': [2568, 2859, 3109, 3205], 'Tris,': [2571, 2781, 2868, 2911, 2960, 3112, 3195, 3295, 3353], '7.6,': [2573, 2913, 3297, 3355], 'heated': [2575, 3236], '°C': [2578, 3070, 3242], 'min.': [2581, 4074], 'concentrations': [2583], 'cell': [2585], 'lysates': [2586], 'determined': [2588, 3175], 'bicinchoninic': [2590], '(Pierce)': [2593, 3182, 3256], 'SDS-PAGE': [2596], 'blotting.': [2599], 'Parkin-null': [2600], 'genomic': [2605], 'deletion': [2606], 'exon': [2608], 'removing': [2612], 'domain.': [2621], '2F.': [2622], 'Perez': [2624], 'Palmiter,': [2628], 'manuscript': [2629], 'preparation.': [2631], 'parkin-null': [2633, 4258, 4339, 4395, 4451], 'C57Bl6/J': [2639], 'Sv129S': [2641], 'hybrid': [2642], 'background.': [2643], 'Young': [2644, 2716], 'wild': [2645, 2661, 4255, 4392], 'null': [2649, 4314], '4–12': [2656], 'weeks': [2657], 'old,': [2658], '>22': [2665], 'months': [2666], 'old.': [2667], 'Whole': [2668, 3635], 'brains': [2670, 3484, 3637], 'dissected': [2672], 'studies.': [2676], 'samples': [2680, 2768, 3211, 3234], 'subjects': [2683, 2711], 'summarized': [2691, 2707], 'Table': [2693, 4319], 'I.': [2694, 3427, 3548, 3556, 3682], 'Two': [2695], 'methods': [2697, 3902], '(I': [2698], 'II)': [2700], 'tissues': [2705, 2941], 'below.Table': [2708], 'ISummary': [2709], 'study': [2715, 3410], '(Y)': [2717], '(A)': [2720], 'cases': [2721, 3509, 3892], 'according': [2724, 4144], 'pathologically': [2727], 'diagnoses,': [2729], 'age': [2730], '(Age),': [2733], 'sex': [2734], '(PMI).': [2738], 'follows:': [2743], 'NML,': [2744], 'normal;': [2745], 'SCHZ,': [2746], 'schizophrenia;': [2747], 'DLB,': [2748], 'bodies;': [2752], 'MSA,': [2753], 'atrophy.CaseDiagnosisAgeSexPMIyearshY-1NML14M10Y-2NML23M8Y-3NML22M4A-1SCHZ86F7.5A-2NML92F5A-3SCHZ83F7.5A-4SCHZ70F17.5A-5NML69M11A-6SCHZ76F10A-7NML60M14A-8NML74F6A-9NML74F3.5A-10NML43M30.5A-11DLB90F5A-12DLB75F6A-13DLB79M20.5A-14NML75M17A-15MSA65M43A-16MSA57F8A-17MSA54M25': [2756], 'Open': [2757], 'table': [2758], 'new': [2761], 'tab': [2762], 'Method': [2763, 2939], 'I—Murine': [2764], 'homogenized': [2770, 2950, 3052, 3287], 'ml/g': [2773, 2841, 2852, 2904, 2953, 3010, 3055, 3105], '(HS)': [2777], '(50': [2779], '7.5,': [2783, 2962], 'EDTA,': [2786, 2965, 3200], '750': [2787], 'NaCl)': [2789, 3023, 3065], 'sedimented': [2791, 2920, 2973, 3025], '125,000': [2793, 2922, 3304], '30': [2797, 2926, 2979, 3031, 3085, 3123], 'Supernatants': [2802, 2871, 2984, 3036, 3090, 3128], 'initial': [2805], 'saved': [2808, 2873, 2934, 2986, 3044, 3092, 3130], 'HS': [2811, 2843, 2888], 'fraction,': [2812, 2990], 'pellets': [2815, 2992, 3001, 3100], 're-extracted': [2819], 'sequentially': [2820], 'strengths.': [2827], 'For': [2828, 3164, 4038], 'buffer,': [2830], 'cycles': [2832], 'washes': [2836], '+': [2844, 2889, 3016, 3020, 3059, 3062], 'X-100': [2847, 3019, 3349, 4069], '(HST)': [2848], 'RIPA': [2854, 2878, 2897], '(150': [2855], 'NaCl,': [2857, 4179], '0.1%': [2858], '0.5%': [2860], 'sodium': [2861], 'deoxycholate,': [2862], 'P-40,': [2865], '8.0).': [2870], 'HST': [2876], 'fractions,': [2879], 'Floatation': [2881], 'removal': [2883], 'contaminating': [2885], 'myelin': [2886, 3275], '20%': [2890], 'sucrose': [2891], 'performed': [2893, 3333, 3520, 3955, 4199], 'extraction.': [2898], 'final': [2900], 'SDS': [2907, 2937, 3106, 3133, 3190, 3226, 3231, 3372], 'pellet': [2918, 3285, 4183], '22': [2929, 3069, 3088, 3126], 'fraction.': [2938, 3048, 3098, 3134], 'II—Brain': [2940], 'control': [2947], '(LS)': [2956], '(10': [2958, 3193], 'dithiothreitol,': [2968, 3203], 'sucrose)': [2971, 3207], '25,000': [2975, 3119], 'LS': [2989, 2998], 're-extraction': [2996], 'subjected': [3003], 'sequential': [3006], 'extractions': [3007, 3042, 3271], 'X': [3012], '(TX)': [3013], '(LS': [3015, 3058], '180,000': [3027, 3081], 'TX': [3047], 'Pellets': [3049], 'Sarkosyl': [3056, 3095, 3219], 'N-lauroylsarcosine': [3061], 'incubated': [3067, 3316], 'shaker': [3073], 'h': [3076], 'sedimentation': [3079, 3302], 'Remaining': [3099], 'extracted': [3102, 3263], '2.5': [3104], '(2%': [3108], '7.6)': [3114], 'centrifugation': [3117, 4185], 'All': [3135, 3233], 'contained': [3138], 'mixture': [3140], 'protease': [3142, 3299], 'inhibitors': [3143], '(1': [3144], 'μg/ml': [3145, 4161], 'leupeptin,': [3148], 'pepstatin': [3149], 'A,': [3150], 'N-p-tosyl-l-phenylalanine': [3151], 'chloromethyl': [3152, 3155], 'ketone,': [3153, 3156], 'Nα-p-tosyl-l-lysine': [3154], 'soybean': [3157], 'trypsin': [3158], 'inhibitor,': [3159], 'PMSF).': [3163], 'experiments': [3165], 'quantitative': [3167], 'amounts': [3168], 'total': [3171], 'concentration': [3173], 'BCA': [3178], 'kit': [3181, 3255], 'albumin': [3186], 'standard.': [3189], 'sample': [3191, 3223, 3373], '6.8,': [3197], '40': [3201], 'added': [3209, 3228, 3376], 'HS,': [3213], 'HST,': [3214, 3273], 'RIPA,': [3215], 'LS,': [3216], 'TX,': [3217], 'fractions;': [3222], 'samples.': [3232], 'SDS-PAGE.': [3245], 'PRK8-coupled': [3246, 3321], 'dextran': [3247], 'beads': [3248, 3322, 3338], 'Carbo-Link': [3253], "manufacturer's": [3259, 4147], 'protocol.': [3260], 'frontal': [3266], 'cortex': [3267, 3477], 'floated': [3277], 'removed': [3279], 'above.': [3282, 4202], '8': [3289], 'urea': [3291, 3314], 'inhibitors.': [3300], 'Following': [3301, 4164, 4192], 'g,': [3306, 4189], 'diluted': [3310], 'gentle': [3318, 3367], 'rocking': [3319, 3368], 'h.': [3325, 3371], 'incubation': [3327], 'all': [3329, 3505], 'subsequent': [3330], 'steps': [3331], '3': [3341], 'times': [3342], 'urea,': [3346, 3920], 'bound': [3357], 'Pierce': [3363], 'ImmunoElution': [3364], 'Buffer': [3365], 'aliquot': [3379], 'eluant.': [3382], 'Samples': [3383], 'boiled': [3386], 'limit': [3388], 'carbamoylation': [3389], 'presence': [3394], 'urea.': [3396], 'harvesting,': [3398], 'fixation,': [3399], 'further': [3401], 'processing': [3402], 'specimens': [3406], '(24Duda': [3416, 3671], 'Giasson': [3418, 3673, 3735, 3766, 3802], 'Gur': [3420, 3675, 3770], 'T.L.': [3421, 3676, 3771], 'Montine': [3422, 3677], 'T.J.': [3423, 3678], 'Robertson': [3424, 3553, 3679], 'Biaggioni': [3426, 3681], 'Hurtig': [3428, 3683, 3772], 'H.I.': [3429, 3684, 3773], 'Stern': [3430, 3685, 3774], 'M.B.': [3431, 3686, 3775], 'Gollomp': [3432, 3687, 3776], 'Grossman': [3434, 3689], 'Neuropathol.': [3441, 3696], 'Exp.': [3442, 3697], '830-841Crossref': [3446, 3701], '(136)': [3449, 3704], '25Schmidt': [3452, 3707], 'Murray': [3454, 3709], 'Hill': [3458, 3713], 'W.D.': [3459, 3714], 'Wertkin': [3460, 3715], '1991;': [3467, 3722], '139:': [3468, 3723], '53-65PubMed': [3469, 3724], 'Briefly,': [3472], 'blocks': [3474], 'cingulate': [3476], 'DLB': [3479, 3506], 'cerebellum': [3481], 'MSA': [3483, 3508], 'fixed': [3486, 3644, 4053, 4173], '70%': [3488, 3646, 4175], 'ethanol,': [3489, 3647, 4176], '150': [3490, 3648, 4177], 'NaCl': [3492, 3650], 'neutral': [3494], 'buffered': [3495], 'formalin': [3496], 'infiltrated': [3498], 'paraffin.': [3500], 'diagnostic': [3502], 'assessment': [3503], '(both': [3510], 'which': [3512], 'characterized': [3515], 'inclusions)': [3518], 'accordance': [3522], 'guidelines': [3525], '(26Gilman': [3526], 'Low': [3528], 'P.A.': [3529], 'Quinn': [3530, 3597], 'Albanese': [3532], 'Ben': [3534], 'Shlomo': [3535], 'Fowler': [3537], 'C.J.': [3538, 3550], 'Kaufmann': [3539], 'Klockgether': [3541], 'Lang': [3543], 'A.E.': [3544], 'Lantos': [3545], 'P.L.': [3546], 'Litvan': [3547], 'Mathias': [3549], 'Oliver': [3551], 'Schatz': [3555], 'Wenning': [3557], 'G.K.': [3558, 3621], '163:': [3563], '94-98Abstract': [3564], '(975)': [3572], '37McKeith': [3575], 'I.G.': [3576], 'Galasko': [3577], 'Kosaka': [3579], 'Perry': [3581, 3624], 'E.K.': [3582], 'Dickson': [3583], 'Hansen': [3585], 'L.A.': [3586], 'Salmon': [3587], 'Lowe': [3589], 'Mirra': [3591], 'S.S.': [3592], 'Byrne': [3593], 'E.J.': [3594], 'Lennox': [3595], 'N.P.': [3598], 'Edwardson': [3599], 'J.A.': [3600], 'Ince': [3601], 'P.G.': [3602], 'Bergeron': [3603], 'Burns': [3605], 'Miller': [3607], 'B.L.': [3608], 'Lovestone': [3609], 'Collerton': [3611], 'E.N.': [3614], 'Ballard': [3615], 'de': [3617], 'Vos': [3618], 'R.A.': [3619], 'Wilcock': [3620], 'Jellinger': [3622], 'K.A.': [3623], 'R.H.': [3625], 'Neurology.': [3626], '1996;': [3627], '47:': [3628], '1113-1124Crossref': [3629], '(3676)': [3632], '(wild': [3638], 'null)': [3642], 'paraffin-embedded.': [3652, 4191], 'Immunohistochemistry': [3653], 'carried': [3655], 'out': [3656, 4269], 'avidin-biotin': [3659], 'complex': [3660], 'detection': [3661], '(Vector': [3663, 4104], 'Burlingame,': [3665, 4106], '3,3′-diaminobenzidine': [3668], 'anti-synuclein': [3728], 'Syn303': [3732], '(28Duda': [3733], 'Mabon': [3737, 3804], 'M.E.': [3738, 3805], '52:': [3746, 3813], '205-210Crossref': [3747, 3814], '(282)': [3750, 3817], 'inclusions': [3758, 3888, 4035], 'immunohistochemistry': [3760], '(27Duda': [3764], 'Chen': [3768], 'Q.': [3769], 'Ischiropoulos': [3778], '157:': [3788], '1439-1445Abstract': [3789], '(241)': [3797], '28Duda': [3800], '29Forman': [3820], 'Sections': [3873], 'lightly': [3876], 'counter-stained': [3877], 'hematoxylin.': [3879], 'order': [3881], 'try': [3883], 'stain': [3885], 'pathological': [3887], 'diseased': [3891], 'variety': [3897], 'fixation': [3899], 'retrieval': [3901, 3918], 'applied.': [3904], 'These': [3905, 4321], 'include': [3906], 'following:': [3908], '1)': [3909], 'formalin-': [3910, 3927], 'ethanol-fixed,': [3912], 'paraffin-infiltrated': [3913], 'antigen': [3917], 'microwave': [3921], 'irradiation,': [3922], 'formic': [3924], 'acid;': [3925], '2)': [3926], 'ethanol-fixed': [3929], 'floating': [3930], 'sections;': [3931], '3)': [3932], 'frozen': [3933, 3943], 'sections': [3934, 3953], 'post-fixed': [3935], 'ethanol': [3937], 'formalin;': [3939], '4)': [3941], 'fresh': [3942], 'un-fixed': [3944], 'sections.': [3945], 'Double': [3946], 'labeling': [3947], 'indirect': [3948], 'immunofluorescence': [3949, 4196], 'anti-α-syn': [3989], 'SNL4': [3991], 'several': [4018], 'examine': [4026], 'co-localization': [4028], 'immunofluorescence.': [4037], 'culture': [4040], 'experiments,': [4041], 'glass': [4046], 'coverslips,': [4047], 'transfected': [4048, 4130, 4151], 'above,': [4051], '4%': [4058], 'paraformaldehyde': [4059], 'PBS': [4061, 4071], 'permeabilization': [4065], '0.2%': [4067], 'Alexa': [4075, 4079], 'Fluor': [4076, 4080], '488-(green)': [4077], '594': [4081], '(red)-conjugated': [4082], '(Molecular': [4085], 'Probes,': [4086], 'Eugene,': [4087], 'OR)': [4088], 'immunostaining,': [4093], 'slides': [4095], 'coverslipped': [4097], 'Vectashield': [4099], '4′,6-diamidino-2-phenylindole': [4100], '(DAPI)': [4101], 'mounting': [4102], 'CA).': [4107], 'medium,': [4115], 'plasmid': [4133], 'parkin/pcDNA': [4134, 4153], '3.1': [4135, 4154], 'un-tagged': [4138], 'LipofectAMINE': [4141], 'instructions.': [4148], 'selected': [4156, 4457], '200': [4160], 'geneticin': [4162], 'harvesting': [4165], 'trypsin,': [4167], 'PBS,': [4172], 'compacted': [4180], '13,000': [4187], 'sectioning,': [4193], 'immunocytochemistry': [4194], 'staining': [4197], 'battery': [4210], 'raised': [4215], 'against': [4216], 'under': [4222], '"Experimental': [4223], 'Procedures."': [4224], 'We': [4225], '60': [4227, 4274], 'hybridomas': [4228], 'strong': [4230], 'immunoreactivity': [4231], 'ELISA.': [4237], 'confirm': [4239], 'mAbs,': [4245, 4275, 4404], 'blot': [4249], 'extracts': [4253, 4263, 4311, 4333, 4337, 4363, 4398, 4442, 4449], 'Surprisingly,': [4268], 'our': [4271], 'four': [4277, 4322], '(PRK8,': [4280], 'PRK28,': [4281, 4366], 'PRK106,': [4282, 4368], 'PRK109)': [4284], 'able': [4286], 'apparent': [4292], 'masses': [4294], 'corresponding': [4295], 'while': [4300], 'time': [4304], 'showing': [4305], 'no': [4306], 'cross-reactivity': [4307], '(Fig.': [4316, 4416, 4453], 'II).': [4320], '∼50-': [4326, 4353], '∼44-kDa': [4328], 'mice;': [4340], 'representative': [4341], 'blots': [4343], '1B.': [4348], 'similar': [4350], 'doublet': [4351], '∼46-kDa': [4355], 'immunobands': [4356], 'PRK8,': [4365], 'PRK35,': [4367], 'PRK109,': [4369], 'commercially': [4372, 4420], 'available': [4373], 'antibody,': [4374], '(Figs.': [4376], '1B': [4377], '2).': [4379], 'very': [4381], 'minor': [4382], 'cross-reacting': [4383], 'band': [4384, 4408], '∼142': [4386], '∼142-kDa': [4407], 'species': [4412], 'recognized': [4413], '1B).': [4417], 'did': [4425], 'authentic': [4428], 'since': [4430], '1B).Table': [4454], 'IICharacterization': [4455], 'antibodiesAntibodyDomainaUbl,': [4459], 'domain;': [4461, 4464, 4468], 'Md,': [4462], 'IBR,': [4465], 'RINGs': [4467], 'R2,': [4469], 'RI': [4471]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2069403245', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 6}, {'year': 2022, 'cited_by_count': 5}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 8}, {'year': 2019, 'cited_by_count': 7}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 11}, {'year': 2013, 'cited_by_count': 6}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-12-08T17:44:52.998909', 'created_date': '2016-06-24'}
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