Publication Information

{'id': 'https://openalex.org/W2067682854', 'doi': 'https://doi.org/10.1055/s-2007-996066', 'title': 'Measurement of Fluorescent-Labeled LMM-Heparin in Biological Fluids Using Protamine-Linked Microbeads', 'display_name': 'Measurement of Fluorescent-Labeled LMM-Heparin in Biological Fluids Using Protamine-Linked Microbeads', 'publication_year': 1997, 'publication_date': '1997-02-01', 'ids': {'openalex': 'https://openalex.org/W2067682854', 'doi': 'https://doi.org/10.1055/s-2007-996066', 'mag': '2067682854', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9156407'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1055/s-2007-996066', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S96726197', 'display_name': 'Seminars in Thrombosis and Hemostasis', 'issn_l': '0094-6176', 'issn': ['0094-6176', '1098-9064'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320000', 'host_organization_name': 'Thieme Medical Publishers (Germany)', 'host_organization_lineage': ['https://openalex.org/P4310320000'], 'host_organization_lineage_names': ['Thieme Medical Publishers (Germany)'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'review', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5079538925', 'display_name': 'Reinhard Malsch', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I223822909', 'display_name': 'Heidelberg University', 'ror': 'https://ror.org/038t36y30', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I223822909']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Reinhard Malsch', 'raw_affiliation_strings': ['1st Department of Medicine, Faculty of Clinical Medicine, Mannheim, University of Heidelberg, Germany.'], 'affiliations': [{'raw_affiliation_string': '1st Department of Medicine, Faculty of Clinical Medicine, Mannheim, University of Heidelberg, Germany.', 'institution_ids': ['https://openalex.org/I223822909']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5098016101', 'display_name': 'Daniel Melui', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Daniel Melui', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5079697983', 'display_name': 'Lukas Piazolo', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Lukas Piazolo', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5074142514', 'display_name': 'Job Harenberg', 'orcid': 'https://orcid.org/0000-0003-4590-4225'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Job Harenberg', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5009889446', 'display_name': 'D. L. Heene', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Dieter Heene', 'raw_affiliation_strings': [], 'affiliations': []}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 1, 'citation_normalized_percentile': {'value': 0.021368, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 55, 'max': 62}, 'biblio': {'volume': '23', 'issue': '01', 'first_page': '23', 'last_page': '30'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11131', 'display_name': 'Proteoglycans and glycosaminoglycans research', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11131', 'display_name': 'Proteoglycans and glycosaminoglycans research', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12352', 'display_name': 'Heparin-Induced Thrombocytopenia and Thrombosis', 'score': 0.9958, 'subfield': {'id': 'https://openalex.org/subfields/2746', 'display_name': 'Surgery'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10746', 'display_name': 'Platelet Disorders and Treatments', 'score': 0.988, 'subfield': {'id': 'https://openalex.org/subfields/2720', 'display_name': 'Hematology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/protamine-sulfate', 'display_name': 'Protamine sulfate', 'score': 0.41524014}], 'concepts': [{'id': 'https://openalex.org/C2780835560', 'wikidata': 'https://www.wikidata.org/wiki/Q5449573', 'display_name': 'Protamine', 'level': 3, 'score': 0.95211744}, {'id': 'https://openalex.org/C2777557582', 'wikidata': 'https://www.wikidata.org/wiki/Q190016', 'display_name': 'Heparin', 'level': 2, 'score': 0.7818911}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.6251143}, {'id': 'https://openalex.org/C553184892', 'wikidata': 'https://www.wikidata.org/wiki/Q1141429', 'display_name': 'Flow cytometry', 'level': 2, 'score': 0.62244606}, {'id': 'https://openalex.org/C1621761', 'wikidata': 'https://www.wikidata.org/wiki/Q7997549', 'display_name': 'Whole blood', 'level': 2, 'score': 0.58539885}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.57558894}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.46672717}, {'id': 'https://openalex.org/C2777397205', 'wikidata': 'https://www.wikidata.org/wiki/Q275792', 'display_name': 'Saline', 'level': 2, 'score': 0.4583378}, {'id': 'https://openalex.org/C2779354726', 'wikidata': 'https://www.wikidata.org/wiki/Q22075855', 'display_name': 'Protamine sulfate', 'level': 4, 'score': 0.41524014}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.2854173}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.26272783}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.18287882}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.13794768}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.10109162}, {'id': 'https://openalex.org/C134018914', 'wikidata': 'https://www.wikidata.org/wiki/Q162606', 'display_name': 'Endocrinology', 'level': 1, 'score': 0.086343765}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C62520636', 'wikidata': 'https://www.wikidata.org/wiki/Q944', 'display_name': 'Quantum mechanics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D005434', 'descriptor_name': 'Flow Cytometry', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D006495', 'descriptor_name': 'Heparin, Low-Molecular-Weight', 'qualifier_ui': 'Q000097', 'qualifier_name': 'blood', 'is_major_topic': True}, {'descriptor_ui': 'D001667', 'descriptor_name': 'Binding, Competitive', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005434', 'descriptor_name': 'Flow Cytometry', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016650', 'descriptor_name': 'Fluorescein-5-isothiocyanate', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005456', 'descriptor_name': 'Fluorescent Dyes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006495', 'descriptor_name': 'Heparin, Low-Molecular-Weight', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008863', 'descriptor_name': 'Microspheres', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011479', 'descriptor_name': 'Protamines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1055/s-2007-996066', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S96726197', 'display_name': 'Seminars in Thrombosis and Hemostasis', 'issn_l': '0094-6176', 'issn': ['0094-6176', '1098-9064'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320000', 'host_organization_name': 'Thieme Medical Publishers (Germany)', 'host_organization_lineage': ['https://openalex.org/P4310320000'], 'host_organization_lineage_names': ['Thieme Medical Publishers (Germany)'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9156407', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/6', 'score': 0.65, 'display_name': 'Clean water and sanitation'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W2462737584', 'https://openalex.org/W2415002397', 'https://openalex.org/W2411036744', 'https://openalex.org/W2328522296', 'https://openalex.org/W2111990318', 'https://openalex.org/W2027795091', 'https://openalex.org/W2025062353', 'https://openalex.org/W2023619243', 'https://openalex.org/W1980832026', 'https://openalex.org/W1964253022'], 'abstract_inverted_index': {'A': [0], 'quantitative': [1], 'assay': [2, 276], 'for': [3, 49, 156, 277], 'fluorescent': [4, 53, 109], 'heparin': [5, 83, 110, 278], 'in': [6, 11, 38, 124, 161, 176, 180, 207, 214, 221, 279], 'a': [7, 29, 34, 256, 273], 'purified': [8], 'system': [9], 'and': [10, 33, 70, 77, 84, 102, 111, 128, 153, 163, 179, 216, 259], 'plasma': [12, 162], 'was': [13, 43, 172], 'developed': [14], '(Piazolo': [15], 'et': [16], 'al:': [17], 'Semin': [18], 'Thromb': [19], 'Hemostas': [20], '20:227-235,': [21], '1994).': [22], 'The': [23, 55, 90, 105, 117, 165, 191], 'protamine': [24, 47, 61, 91, 106, 118, 146, 189, 227], 'microbeads': [25, 48, 62, 81, 107, 119, 136, 200, 228, 248], '(1.6': [26], 'microns)': [27, 72, 243], 'showed': [28], 'broad': [30], 'size': [31, 254], 'distribution': [32], 'large': [35], 'standard': [36], 'variation': [37], 'low': [39], 'concentrations.': [40], 'Our': [41], 'aim': [42], 'to': [44, 142, 188, 204, 211, 218, 232, 271], 'optimize': [45], 'these': [46], 'the': [50, 94, 97, 135, 143, 148, 151, 154, 157, 169, 197, 234], 'measurement': [51], 'of': [52, 63, 96, 137, 145, 150, 159, 168, 194, 196, 236, 250], 'heparin.': [54], 'following': [56], 'results': [57], 'were': [58, 73, 112, 185], 'obtained:': [59], 'Paramagnetic': [60], 'different': [64, 138, 198], 'average': [65], 'diameters': [66], '(0.8,': [67], '1.6,': [68], '2.8,': [69], '4.5': [71, 242], 'synthesized': [74], 'by': [75, 114], 'cyclocarbodiimide': [76], 'tosyl': [78], 'activation.': [79], 'These': [80], 'bind': [82], 'are': [85, 131, 268], 'assayed': [86], 'using': [87], 'flow': [88, 115], 'cytometry.': [89, 116], 'concentration': [92, 235], 'on': [93], 'surface': [95], 'beads': [98], 'ranged': [99, 201], 'between': [100, 134], '2.0': [101], '61': [103], 'mg/mL.': [104], 'bound': [108, 120, 187], 'analyzed': [113], 'LMM-heparin-tyramine-FITC': [121], 'dose': [122], 'dependently': [123], 'saline': [125, 177, 208], 'solution,': [126, 209], 'plasma,': [127, 215], 'blood.': [129, 164, 222], 'There': [130], 'substantial': [132], 'differences': [133], 'origins': [139], 'with': [140, 265], 'regard': [141], 'amount': [144], 'bound,': [147], 'sensitivity': [149, 167], 'detection,': [152], 'reliability': [155], 'determination': [158], 'heparins': [160], 'minimal': [166], 'final': [170], 'method': [171], '0.001': [173], 'U/mL': [174], 'LMMH-tyramine-FITC': [175, 195], 'solution': [178], 'plasma.': [181, 280], 'Human': [182], 'blood': [183], 'cells': [184], 'not': [186], 'microbeads.': [190], 'half-maximal': [192], 'binding': [193, 275], 'protamine-coated': [199], 'from': [202], '1.7': [203], '8.0': [205], 'micrograms/mL': [206, 213, 220], '2.3': [210], '8.7': [212], '3.1': [217], '6.4': [219], 'We': [223], 'conclude': [224], 'that': [225], 'all': [226], 'can': [229, 261], 'be': [230, 262], 'used': [231, 270], 'quantify': [233], 'LMMH-tyramine.': [237], 'Protamine': [238], 'Dynabeads': [239], 'M-450': [240], '(diameter': [241], 'have': [244], 'advantages': [245], 'over': [246], 'other': [247], 'because': [249], 'their': [251], 'more': [252], 'homogeneous': [253], 'distribution,': [255], 'higher': [257], 'selectivity,': [258], 'they': [260], 'measured': [263], 'together': [264], 'leukocytes.': [266], 'They': [267], 'currently': [269], 'develop': [272], 'competitive': [274]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2067682854', 'counts_by_year': [], 'updated_date': '2024-12-15T23:34:11.292060', 'created_date': '2016-06-24'}