Title: Use of capillary zone electrophoresis in the determination of B vitamins in pharmaceutical products
Abstract: The simultaneous determination of several water-soluble vitamins is difficult and often many different analyses have to be done. Capillary zone electrophoresis (CZE) has been claimed to provide a sensitive and high-resolution method for the determination of different kinds of biomolecules. In this study the B vitamins thiamine (B1), riboflavin (B2, pyridoxal (B6, pyridoxine (B6, and pyridoxamine (B6, in a pharmaceutical product were determined simultaneously using CZE. An HCl solution was used for the extraction of the vitamins from a multivitamin-multimineral tablet. Electrophoretic experiments were performed with ISCO Model 3850 apparatus equipped with a UV detector. The applied potential was 6.0 kV and 75-μm fused-silica capillary tubing was used. A 5- μl sample was injected via split injection. The electrolyte was 0.020 M sodium phosphate buffer (pH 9.0). Vitamins were recorded at 254 nm. Preliminary results clearly pointed out the feasibility of the simultaneous determination of water-soluble B vitamins in pharmaceutical products by CZE. The relative standard deviation, however, varied from 2.1 to 6.3%.
Publication Year: 1993
Publication Date: 1993-04-01
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 41
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot