Title: Preparative thin-layer chromatography for the separation of the various forms of vitamin B6 in tissues
Abstract: 1. A method is described whereby total pyridoxal, pyridoxine, pyridoxamine and 4-pyridoxic acid have been quantitatively separated and measured using one tissue sample. The method utilizes preparative thin-layer chromatography for separation of the previously mentioned forms of vitamin B6. After elution, the pyridoxic acid lactone method was employed for fluorimetric determination of the concentration of the vitamin forms involved. 2. This method was applied to the determination of vitamin B6 concentration in chick embryo livers ranging from 8 to 14 days of development. From 8 to 12 days the order of compounds (from highest to lowest)_concentration is pyridoxine > pyridoxamine > pyridoxal > pyridoxic acid. At 13 and 14 days the order is pyridoxamine > pyridoxine > pyridoxal > pyridoxic acid. The concentration of total forms of vitamin B6 reaches a maximum at 12 days and thenn decrease significantly. 3. The concentration of pyridoxal phosphate in embryonic chick liver from 8 to 14 days of development has also been determined, and ranges from 4 to 8 nmoles per g liver. The maximum concentration occurs at 12 and 13 days.
Publication Year: 1971
Publication Date: 1971-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 7
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