Phosphatidylinositol 4-OH Kinase Is a Downstream Target of Neuronal Calcium Sensor-1 in Enhancing Exocytosis in Neuroendocrine Cells

Name: Work Video:
Duration: 3 min 30 s
Manisha Rajebhosale; Sam M. Greenwood; Jolanta Vidugirienė; Andreas Jeromin; Sabine Hilfiker
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704, 715, 746, 761, 774, 782, 817, 925, 937, 1052, 1229, 1243, 1267, 1270, 1334, 1508, 1535, 1542, 1690, 1705, 1731, 1734, 1746, 1751, 1765, 1894, 1910, 1934, 2082, 2121, 2136, 2143, 2191, 2302, 2305, 2313, 2328, 2341, 2359, 2362, 2366, 2398, 2406, 2416, 2432, 2444, 2458, 2521, 2581, 2887, 2896, 2913, 2960, 2968, 2980, 2990, 3105, 3353, 3398, 3403, 3477, 3495, 3586, 3596, 3625, 3743, 3779, 3797, 3849, 4001, 4101, 4161, 4187, 4195, 4198, 4224, 4312, 4342, 4368], 'mammalian': [5, 200, 449, 1335], 'orthologue': [6, 201], 'of': [7, 13, 40, 84, 93, 95, 103, 153, 182, 188, 193, 202, 208, 235, 279, 288, 290, 298, 348, 377, 383, 388, 451, 458, 493, 515, 523, 563, 579, 612, 690, 703, 754, 757, 763, 770, 784, 809, 857, 951, 961, 965, 1054, 1131, 1422, 1432, 1461, 1510, 1539, 1707, 1710, 1714, 1722, 1733, 1742, 1748, 1767, 1795, 1897, 1913, 1939, 2062, 2067, 2069, 2076, 2166, 2194, 2203, 2304, 2348, 2361, 2365, 2447, 2527, 2529, 2539, 2562, 2591, 2713, 2746, 2889, 2891, 2898, 2900, 2905, 2932, 2954, 2962, 2970, 2992, 3019, 3100, 3104, 3307, 3343, 3427, 3431, 3437, 3469, 3497, 3560, 3585, 3616, 3701, 3708, 3733, 3752, 3800, 3958, 4016, 4103, 4144, 4160, 4175, 4180, 4182, 4197, 4200, 4212, 4223, 4238, 4314, 4344, 4351], 'frequenin,': [8, 203, 452], 'belongs': [9, 204, 453], 'to': [10, 21, 56, 147, 168, 185, 205, 216, 251, 342, 363, 380, 454, 510, 591, 616, 625, 636, 677, 695, 709, 767, 787, 903, 924, 972, 978, 1048, 1051, 1066, 1127, 1251, 1259, 1297, 1353, 1468, 1534, 1596, 1598, 1618, 1663, 1719, 1736, 1760, 1769, 1787, 1933, 2081, 2094, 2146, 2310, 2358, 2431, 2600, 2849, 3009, 3164, 3175, 3189, 3255, 3485, 3506, 3681, 3695, 3817, 4000, 4193, 4234, 4310, 4324, 4332, 4341, 4364], 'a': [11, 74, 87, 121, 206, 269, 282, 316, 542, 551, 572, 682, 711, 751, 1129, 1470, 1485, 1511, 1578, 1667, 1792, 1926, 2164, 2233, 2250, 2273, 2377, 2382, 2530, 2661, 3198, 3205, 3223, 3252, 3327, 3341, 3364, 3579, 3853, 3935, 4141, 4168, 4173, 4213, 4236, 4306, 4336, 4357], 'family': [12, 207, 457, 531, 566], 'EF-hand-containing': [14, 156, 209, 351], 'Ca2+': [15, 75, 81, 157, 210, 270, 276, 352, 623, 675, 785, 1137, 2043, 4352, 4365], 'sensors.': [16, 211], 'NCS-1/frequenin': [17, 212, 941, 968, 1055], 'has': [18, 213, 545, 900, 954, 969, 1294, 1350, 1465, 1532, 1615, 4230], 'been': [19, 214, 546, 901, 955, 970, 1295, 1351, 1466, 1616, 1845, 3112, 4231], 'shown': [20, 215, 902, 971, 1296, 1352, 1467, 1617], 'enhance': [22, 169, 217, 364], 'synaptic': [23, 218, 933], 'transmission': [24, 219], 'in': [25, 89, 140, 144, 220, 284, 335, 339, 504, 512, 536, 548, 558, 621, 687, 781, 811, 890, 931, 1040, 1056, 1058, 1234, 1357, 1364, 1574, 1590, 1600, 1608, 1623, 1757, 2000, 2003, 2040, 2085, 2152, 2281, 2296, 2322, 2327, 2340, 2345, 2392, 2397, 2415, 2439, 2486, 2489, 2580, 2613, 2655, 2706, 2720, 2733, 2776, 2782, 2806, 2815, 2819, 2826, 2834, 2838, 2853, 2865, 2869, 3338, 3411, 3542, 3548, 3557, 3592, 3629, 3789, 3804, 3848, 3852, 3887, 3934, 3938, 3945, 3947, 3950, 4029, 4135, 4207, 4226, 4240, 4318], 'PC12': [26, 46, 221, 241, 894, 1701, 1974, 2129, 2463, 2534, 3407, 3805, 3820, 3959, 4031, 4150, 4328, 4399], 'cells': [27, 222, 506, 1366, 1702, 1735, 1975, 2026, 2130, 2339, 2464, 2480, 2517, 2535, 2556, 2752, 2906, 2963, 2975, 2993, 2999, 3297, 3345, 3408, 3462, 3498, 3527, 3539, 3860, 3940, 3960, 4032, 4189, 4329, 4400], 'andDrosophila': [28, 223], 'and': [29, 80, 190, 224, 275, 385, 489, 499, 507, 538, 571, 609, 855, 866, 893, 977, 1060, 1077, 1126, 1197, 1241, 1261, 1265, 1359, 1417, 1434, 1484, 1588, 1611, 1659, 1724, 1744, 1791, 1833, 1843, 1877, 1885, 1902, 1906, 1951, 2013, 2021, 2044, 2046, 2064, 2092, 2108, 2141, 2154, 2185, 2263, 2267, 2312, 2317, 2356, 2381, 2387, 2443, 2505, 2536, 2566, 2598, 2632, 2678, 2693, 2698, 2749, 2771, 2824, 2874, 2902, 2920, 2922, 2967, 2997, 3012, 3088, 3094, 3108, 3184, 3209, 3229, 3242, 3277, 3340, 3367, 3397, 3442, 3474, 3488, 3533, 3535, 3572, 3624, 3632, 3687, 3698, 3727, 3742, 3802, 3812, 3825, 3833, 3842, 3851, 3879, 3925, 3941, 3994, 4056, 4085, 4093, 4105, 4116, 4140, 4165, 4302, 4316, 4346, 4356], 'Xenopus,': [30, 225], 'respectively.': [31, 226, 1888], 'However,': [32, 227, 741, 936, 1038, 1607], 'precise': [34, 229], 'molecular': [35, 230, 1244], 'mechanism': [36, 231], 'for': [37, 232, 555, 928, 957, 1232, 1239, 1300, 1518, 1580, 2148, 2169, 2298, 2319, 2533, 2542, 2575, 2626, 2671, 2727, 2761, 2773, 2796, 2821, 2840, 2856, 2871, 2879, 2909, 2924, 3002, 3348, 3439, 3608, 3721, 3858, 4083, 4170, 4220], 'enhancement': [39, 192, 234, 387, 1721, 1794], 'exocytosis': [41, 50, 70, 236, 245, 265, 906, 1460, 1519, 1624, 4171, 4241], 'is': [42, 237, 596, 707, 749, 887, 945, 1570, 1577, 1661, 4156, 4216, 4322], 'largely': [43, 238, 528], 'unknown.': [44, 239, 529], 'In': [45, 240, 699, 1506, 1689, 3822, 3927, 4058, 4152], 'cells,': [47, 242, 540, 896, 1613, 2544, 3806, 3827, 3949, 4154], 'NCS-1': [48, 67, 85, 116, 145, 178, 243, 262, 280, 311, 340, 373, 764, 788, 886, 1063, 1233, 1248, 1356, 1697, 1727, 1758, 1781, 1799, 1915, 2748, 2901, 3014, 3239, 3801, 3829, 4084, 4104, 4315], 'potentiated': [49, 244], 'evoked': [51, 246, 814, 867, 1602], 'by': [52, 72, 247, 267, 939, 1585, 1603, 1840, 1882, 1892, 1900, 2126, 2232, 2249, 2272, 2372, 2585, 2596, 2737, 2847, 2958, 2988, 3493, 3574, 3602, 3729, 3748, 3761, 3786, 4114, 4330], 'ATP,': [53, 248], 'an': [54, 101, 138, 249, 296, 333, 1414, 1571, 1620, 1720, 1740, 2323, 2393, 2422, 2681, 3218, 3449, 3593, 4111, 4221], 'agonist': [55, 250], 'phospholipase': [57, 122, 252, 317, 407, 1581, 3244], 'C-linked': [58, 253], 'receptors,': [59, 254, 1587], 'but': [60, 255, 541, 1712, 2947, 3300], 'had': [61, 256, 1772], 'no': [62, 257, 4061], 'effect': [63, 258, 926, 1776, 4196], 'on': [64, 259, 1043, 1666, 1777, 1981, 2190, 2509, 3197, 3217, 3313, 3894, 3955, 4107, 4202], 'depolarization-evoked': [65, 260], 'release.': [66, 261, 1726], 'also': [68, 263, 888, 1064, 1729], 'enhanced': [69, 264, 417, 1788, 2682], 'triggered': [71, 266], 'ionomycin,': [73, 268], 'ionophore': [76, 271], 'that': [77, 149, 177, 272, 344, 372, 674, 717, 745, 773, 1410, 1537, 1696, 1762, 1780, 2409, 2941, 4013], 'bypasses': [78, 273], 'K+': [79, 274, 1199, 2255], 'channels.': [82, 277], 'Overexpression': [83, 278, 808, 1709], 'caused': [86, 281, 4394], 'shift': [88, 283], 'dose-response': [91, 286], 'curve': [92, 287], 'inhibition': [94, 289], 'ATP-evoked': [96, 291, 2228], 'secretion': [97, 292, 1601, 1699, 1952, 2178, 2229, 2246, 2269, 2738, 2910, 4209, 4326, 4397], 'using': [98, 120, 293, 315, 1925, 1944, 2034, 2073, 2421, 2524, 2546, 2559, 2680, 2938, 3213, 3233, 3326, 3402, 3448, 3772], 'phenylarsine': [99, 294, 410, 1737, 2714], 'oxide,': [100, 295], 'inhibitor': [102, 297, 1741], 'phosphatidylinositol': [104, 110, 299, 305, 403, 443, 446, 1272, 1471], '4-OH': [105, 300, 404, 1273], 'kinase': [106, 301, 981, 1274, 1771, 3561, 3630, 3693], '(PI4K).': [107, 302], 'Plasma': [108, 303], 'membrane': [109, 304, 436, 697, 1544, 3369, 3635, 3688, 4018, 4370], '4,5-bisphosphate': [111, 306], 'pools': [112, 307], 'were': [113, 308, 1837, 1880, 1923, 1954, 1979, 1995, 2032, 2071, 2114, 2139, 2158, 2180, 2198, 2289, 2308, 2343, 2369, 2419, 2437, 2455, 2465, 2481, 2518, 2557, 2569, 2594, 2606, 2658, 2688, 2704, 2753, 2794, 2813, 2863, 2907, 2915, 2986, 3000, 3024, 3194, 3211, 3231, 3236, 3270, 3298, 3319, 3336, 3346, 3358, 3393, 3400, 3409, 3422, 3463, 3510, 3528, 3540, 3546, 3555, 3690, 3746, 3759, 3815], 'increased': [114, 134, 186, 309, 329, 381, 1745], 'upon': [115, 310, 1362, 3294, 3880, 4009, 4210], 'transfection': [117, 312, 2096, 2107, 2122, 2153], 'as': [118, 313, 497, 1072, 1135, 1429, 1803, 1956, 2106, 2335, 2405, 2476, 2550, 2700, 2740, 2893, 2895, 2972, 3279, 3360, 3491, 3621, 3634, 3641, 3778, 3856, 4020, 4167, 4172, 4218, 4305], 'visualized': [119, 314, 2943, 3272], 'C-δ': [123, 318, 3245], 'pleckstrin': [124, 319, 439], 'homology': [125, 320], 'domain-green': [126, 321], 'fluorescent': [127, 322, 414], 'protein': [128, 323, 557, 716, 1473, 2593, 2934, 2945, 2969, 2982, 4143, 4199, 4215, 4225], 'construct.': [129, 324, 3004], 'NCS-1-transfected': [130, 325], 'cell': [131, 326, 2491, 2567, 3318, 3481, 3685], 'extracts': [132, 327], 'displayed': [133, 328], 'phosphatidylinositol-4-phosphate': [135, 330], 'biosynthesis,': [136, 331, 3684], 'indicating': [137, 332, 772], 'increase': [139, 334, 904, 2095], 'PI4K': [141, 336, 1515, 3637], 'activity.': [142, 337], 'Mutations': [143, 338], 'equivalent': [146, 341, 1759, 1911], 'those': [148, 343, 1761], 'abolish': [150, 345], 'interaction': [152, 347], 'recoverin,': [154, 349], 'another': [155, 350], 'sensor,': [158, 353], 'with': [159, 354, 721, 1355, 1764, 1971, 1985, 2008, 2135, 2182, 2200, 2237, 2253, 2276, 2291, 2457, 2497, 2520, 2608, 2634, 2660, 2690, 2696, 2758, 2764, 2802, 2876, 2974, 3238, 3286, 3352, 3424, 3434, 3692, 3791, 3835, 3890, 3952, 4004, 4067, 4087, 4096, 4402], 'its': [160, 355, 898, 1627, 1715, 3891, 3953], 'downstream': [161, 356, 1245, 1420, 1793], 'target': [162, 357, 722, 1421, 1669], 'rhodopsin': [163, 358, 980, 1770], 'kinase,': [164, 359], 'lost': [165, 360, 1773], 'their': [166, 361, 631, 1774], 'ability': [167, 362], 'exocytosis.': [170, 194, 365, 389, 935, 4227], 'Taken': [171, 366], 'together,': [172, 367], 'present': [174, 369, 889, 1691], 'data': [175, 370, 3210, 3230, 3399, 4078], 'indicate': [176, 371], 'modulates': [179, 374], 'activity': [181, 376, 1268, 1516, 1706, 3638], 'PI4K,': [183, 378, 402, 1743, 1785], 'leading': [184, 379, 1786], 'levels': [187, 382, 1592, 1747, 1790, 2414, 2946, 3357], 'phosphoinositides': [189, 384], 'concomitant': [191, 386, 4347], '1The': [394], 'abbreviations': [395], 'used': [396, 2103, 3590, 3816, 4233], 'are:': [397], 'NCS,': [398], 'neuronal': [399, 537, 1610], 'sensor;': [401], 'kinase;': [405], 'PLC,': [406], 'C;': [408], 'PAO,': [409], 'oxide;': [411], 'GFP,': [412], 'green': [413], 'protein;': [415, 437], 'EGFP,': [416], 'GFP;': [418], 'PBS,': [419, 3532], 'phosphate-buffered': [420, 2350], 'saline;': [421], 'hGH,': [422, 1898, 2903], 'human': [423], 'growth': [424, 432], 'hormone;': [425], 'PSS,': [426, 2282], 'physiological': [427, 966, 2204], 'saline': [428, 2205, 2256, 2351], 'solution;': [429], 'NGF,': [430], 'nerve': [431], 'factor;': [433], 'VAMP,': [434], 'vesicle-associated': [435, 1514], 'PH,': [438], 'homology;': [440], 'PtdIns(4,': [441], '5)P2,': [442], '4,5-bisphosphate;': [444], 'PI(4)P-5-kinase,': [445], '4-phosphate-5-kinase': [447], 'homologue': [450, 544, 1337], 'EF-hand': [456, 569, 589], 'Ca2+-binding': [459, 585], 'proteins': [460, 628, 680, 723, 1045, 4089, 4239], '(1Braunewell': [461], 'K.-H.': [462], 'Gundelfinger': [463], 'E.D.': [464], 'Cell': [465, 1442, 1948, 1977, 3263, 4048, 4376], 'Tissue': [466], 'Res.': [467, 1032], '1999;': [468, 1288, 1312, 1329, 1871, 1967, 3153, 4251, 4298], '295:': [469, 1194, 3127], '1-12Google': [470, 480, 606], 'Scholar,': [471, 653, 1016, 1091, 1151, 1162, 1181, 1315, 1383, 1447, 1643, 3129, 3660, 3908, 3977, 4254, 4269, 4284, 4381], '2Burgoyne': [472], 'R.D.': [473, 599, 800, 915, 1144, 1155], 'Weiss': [474, 600, 793], 'J.L.': [475, 601, 794, 1140, 1153], 'Biochem.': [476, 602, 1030, 1638, 1652], 'J.': [477, 603, 801, 823, 837, 843, 916, 985, 999, 1005, 1112, 1145, 1156, 1172, 1284, 1321, 1326, 1343, 1374, 1377, 1397, 1402, 1441, 1527, 1563, 1639, 1677, 1682, 1819, 1824, 1964, 3033, 3058, 3063, 3262, 3654, 3673, 3870, 3873, 3904, 3917, 3973, 3984, 4036, 4047, 4128, 4248, 4278, 4295, 4375], '2001;': [478, 604, 1159, 1178, 1222, 1405, 1444, 1455, 1826, 3066], '353:': [479, 605], 'Scholar),': [481, 854, 922, 1125, 1291, 1408, 1658, 3069, 3878], 'which': [482, 622, 940, 962, 1292, 4136, 4339, 4362, 4405], 'includes': [483], 'recoverin/S-modulin,': [484], 'visinin,': [485, 500], 'visinin-like': [486], 'proteins,': [487, 2996], 'neurocalcin,': [488], 'hippocalcin.': [490], 'Some': [491], 'members': [492, 526, 532, 562, 756], 'this': [494, 556, 758, 2331, 2401, 3617], 'family,': [495, 759], 'such': [496, 1071, 1134], 'recoverin/S-modulin': [498], 'are': [501, 508, 527, 533, 583, 1046, 1236, 1593, 4146], 'expressed': [502, 535], 'only': [503, 959, 2104], 'photoreceptor': [505, 1044], 'thought': [509, 708, 4323], 'function': [511, 1053, 1249, 4174], 'control': [514, 2186, 4403], 'visual': [516], 'transduction': [517], 'pathways,': [518], 'whereas': [519, 587, 2912], 'functional': [521, 584], 'roles': [522], 'other': [525, 2981], 'Most': [530], 'solely': [534], 'neuroendocrine': [539, 895, 1061, 1612], 'frequenin': [543, 810, 858, 930, 1256], 'identified': [547, 956, 3785], 'yeast,': [549], 'suggesting': [550, 1409, 1779, 4012], 'more': [552, 4005], 'general': [553, 752], 'role': [554, 1622, 4313], 'mediating': [559, 1247], 'Ca2+responses.': [560], 'All': [561, 2435], 'NCS': [565, 627, 679], 'share': [567], 'four': [568, 581, 2170], 'motifs': [570], 'myristoylated': [573], 'N': [574, 593], 'terminus.': [575], 'For': [576, 3289], 'NCS-1,': [577, 1711, 2892, 3809], 'three': [578, 1755, 3395], 'EF-hands': [582], 'motifs,': [586], 'closest': [590], 'terminus': [594], '(EF1)': [595], 'non-functional': [597], '(2Burgoyne': [598], 'Scholar).': [607, 670, 740, 807, 885, 1037, 1225, 1332, 1458, 1505, 1688, 1829, 1874, 3156, 3268, 3391, 3679, 4392], 'Biochemical': [608], 'structural': [610], 'analysis': [611], 'recoverin': [613, 1768], 'have': [614, 1426, 1844, 3111], 'led': [615, 1533, 1718], 'calcium/myristoyl': [618, 747], 'switch': [619, 748], 'model': [620, 672, 4308], 'binding': [624, 676, 786, 952, 1230, 1766, 4331], 'may': [629, 777, 963, 1412], 'trigger': [630], 'translocation': [632], 'from': [633, 907, 1626, 1700, 2131, 2330, 2400, 3181, 3321, 4327, 4353, 4398], 'cytosol': [635, 3622, 3850], 'intracellular': [637, 4006, 4354], 'membranes': [638, 2602], '(3Zozulya': [639], 'S.': [640, 648, 880, 1021, 1027, 1098, 1100, 1120, 1208, 1210, 1220, 1556, 1811, 1959, 3380, 4243, 4271], 'Stryer': [641, 662, 732], 'L.': [642, 663, 733, 792, 4038], 'Proc.': [643, 875, 1115, 1215], 'Natl.': [644, 876, 1116, 1216], 'Acad.': [645, 877, 1117, 1217], 'Sci.': [646, 878, 1118, 1218, 1443, 4377], 'U.': [647, 879, 1119, 1219], 'A.': [649, 841, 849, 881, 1003, 1011, 1083, 1085, 1121, 1170, 1185, 1206, 1221, 1376, 1391, 1399, 1675, 1823, 1849, 1862, 3035, 3052, 3060, 3118, 3131, 3144, 3645, 3864], '1992;': [650], '89:': [651], '11569-11573Google': [652], '4Ames': [654], 'J.B.': [655, 725], 'Ishima': [656, 726], 'R.': [657, 727, 839, 1001, 1102, 1108, 1340, 1546, 1853, 1961, 3135, 3971, 4046, 4245], 'Tanaka': [658, 728], 'T.': [659, 729, 827, 872, 989, 1183, 1191, 1401, 1496, 1523, 1554, 3062, 3116, 3124, 3261, 3374, 3386, 3653, 3900, 3912], 'Gordon': [660, 730], 'J.I.': [661, 731], 'Ikura': [664, 734], 'M.': [665, 735, 3649, 3651, 3962, 4258, 4275], 'Nature.': [666, 736, 1479, 1501, 4265, 4388], '1997;': [667, 737, 1346, 1655, 4266], '389:': [668, 738], '198-202Google': [669, 739], 'This': [671, 4228], 'suggests': [673], 'induces': [681], 'large': [683], 'conformational': [684], 'change,': [685], 'resulting': [686, 3597], 'exposure': [689, 2848], 'myristoyl': [692, 705, 775], 'group': [693, 706, 776], 'believed': [694], 'allow': [696], 'attachment.': [698], 'addition,': [700, 1507], 'movement': [702], 'expose': [710], 'hydrophobic': [712], 'pocket': [713], 'within': [714, 3333], 'could': [718], 'then': [719, 2370], 'interact': [720, 1263, 1354], '(4Ames': [724], 'recent': [742], 'studies': [743], 'suggest': [744, 4079], 'not': [750, 946, 1237, 1713, 2411, 3301], 'feature': [753], 'all': [755, 1940, 2030, 2183, 2721, 3013, 4059], 'because': [760], 'localization': [762, 2745, 3799, 3893, 3954, 4077], 'was': [765, 1257, 1801, 1890, 1942, 2102, 2124, 2230, 2247, 2270, 2315, 2333, 2390, 2403, 2410, 2451, 2583, 2935, 2956, 3589, 3599, 3619, 3627, 3639, 3715, 3719, 3740, 3784, 3830, 3846, 3885, 3931, 3942, 3998, 4063, 4070, 4406], 'found': [766], 'be': [768, 778, 964, 1049, 1252, 1298, 1413], 'independent': [769, 1625], 'Ca2+,': [771], 'freely': [779], 'accessible': [780], 'absence': [783], "(5O'Callaghan": [789], 'D.W.': [790], 'Ivings': [791], 'Ashby': [795], 'M.C.': [796], 'Tepikin': [797], 'A.V.': [798], 'Burgoyne': [799, 914, 1143, 1154], 'Biol.': [802, 917, 1146, 1157, 1287, 1311, 1327, 1344, 1378, 1403, 1564, 1683, 1870, 1965, 3064, 3152, 3264, 3655, 3674, 3985, 4049, 4129, 4249, 4279, 4296], 'Chem.': [803, 918, 1147, 1158, 1328, 1345, 1379, 1404, 1565, 1684, 1966, 3065, 3656, 3675, 3986, 4130, 4250, 4280, 4297], '2002;': [804, 1193, 3126, 3657, 3875], '277:': [805, 3658], '14227-14237Google': [806], 'Drosophila': [812, 929], 'facilitates': [813], 'neurotransmission': [815, 868], 'at': [816, 1541, 1750, 1997, 2052, 2163, 2473, 2483, 2571, 2629, 2646, 2650, 2668, 2674, 2724, 2730, 2799, 2843, 2859, 2882, 3516, 3604, 3611, 3724], 'neuromuscular': [818], 'junction': [819], '(6Pongs': [820, 982], 'O.': [821, 983, 1368, 1437, 3981], 'Lindemeier': [822, 984], 'Zhu': [824, 986], 'X.R.': [825, 987], 'Theil': [826, 988], 'Engelkamp': [828, 990], 'D.': [829, 991, 1864, 3146, 3372, 4387], 'Krah-Jentgens': [830, 992], 'I.': [831, 845, 993, 1007], 'Lambrecht': [832, 994], 'H.-G.': [833, 995], 'Koch': [834, 996], 'K.W.': [835, 997], 'Schwemer': [836, 998], 'Rivosecchi': [838, 1000], 'Mallart': [840, 1002, 1084], 'Galceran': [842, 1004], 'Canal': [844, 1006], 'Barbas': [846, 1008], 'J.A.': [847, 1009, 1651, 1673], 'Ferrus': [848, 1010, 1082], 'Neuron.': [850, 1012, 1177, 1454], '1993;': [851, 1013, 1480, 4131], '11:': [852, 1014], '15-28Google': [853, 1015], 'injection': [856], 'into': [859, 1909, 2160, 3466, 3531, 4149, 4158, 4186], 'Xenopus': [860], 'spinal': [861], 'neurons': [862, 1059], 'enhances': [863, 1698], 'both': [864, 1609, 2965, 3823, 4088], 'spontaneous': [865], '(7Olafsson': [869], 'P.': [870, 1029, 1114, 1308, 1387, 1440, 1851, 1855, 3048, 3133, 3137, 3259, 3969], 'Wang': [871, 1279], 'Lu': [873, 1175], 'B.': [874, 1176, 1212, 1815, 3668], '1995;': [882, 1034, 1502, 3987, 4050], '92:': [883], '8001-8005Google': [884], 'adrenal': [891], 'chromaffin': [892, 3859], 'where': [897], 'overexpression': [899, 2890], 'Ca2+-regulated': [905], 'dense-core': [908], 'granules': [909], '(8McFerran': [910], 'B.W.': [911], 'Graham': [912], 'M.E.': [913, 1104], '1998;': [919, 1685, 3265, 3919, 4281], '273:': [920, 1686, 4282], '22768-22772Google': [921], 'analogous': [923], 'reported': [927, 3892], 'enhancing': [932, 1775], 'vesicle': [934], 'mechanism(s)': [938], 'regulates': [942], 'vesicular': [943, 1303], 'release': [944, 4350], 'known.': [947], 'A': [948, 3737, 3841, 4177, 4205], 'wide': [949], 'array': [950], 'partners': [953, 1231], 'NCS-1/frequenin,': [958], 'some': [960, 1972, 3836], 'relevance.': [967], 'activate': [973, 1067], 'membrane-bound': [974], 'guanylate': [975], 'cyclase': [976], 'inhibit': [979], '9De': [1017], 'Castro': [1018, 1095], 'E.': [1019, 1096, 3979], 'Nef': [1020, 1028, 1097, 1113], 'Fiumelli': [1022], 'H.': [1023, 1317, 1821], 'Lenz': [1024], 'S.E.': [1025], 'Kawamura': [1026], 'Biophys.': [1031], 'Commun.': [1033], '216:': [1035], '133-140Google': [1036], 'these': [1039, 1227], 'vitro': [1041, 1358], 'effects': [1042, 4102], 'unlikely': [1047], 'relevant': [1050], 'vivo': [1057, 1235], 'cells.': [1062, 2196, 3821, 4151], 'seems': [1065], 'various': [1068, 2417, 2711], 'calmodulin': [1069], 'targets,': [1070], 'cyclic': [1073], 'nucleotide': [1074], 'phosphodiesterase,': [1075], 'calcineurin,': [1076], 'nitric-oxide': [1078], 'synthase': [1079], '(10Poulain': [1080], 'C.': [1081, 1306, 1395, 1521, 1813, 1860, 3056, 3142, 3898, 3910, 4034], 'Pflugers': [1086], 'Arch.': [1087], '1994;': [1088, 4378, 4389], '427:': [1089], '71-79Google': [1090], '11Schaad': [1092], 'N.C.': [1093], 'De': [1094, 1438, 1865, 3147, 3967], 'Hegi': [1099], 'Hinrichsen': [1101], 'Martone': [1103], 'Ellisman': [1105], 'M.H.': [1106], 'Sikkink': [1107], 'Rusnak': [1109], 'F.': [1110, 1166, 3966, 4260], 'Sygush': [1111], '1996;': [1122, 1528, 3905], '93:': [1123], '9253-9258Google': [1124], 'modulate': [1128], 'variety': [1130, 4237], 'ion': [1132], 'channels': [1133, 1138, 1200], 'voltage-gated': [1136], '(12Weiss': [1139], 'Archer': [1141], 'D.A.': [1142, 1631], '2000;': [1148, 1380, 1566, 3038, 3388], '275:': [1149, 1381, 1567], '40082-40087Google': [1150], '13Weiss': [1152], '276:': [1160, 1406, 3067], '44804-44811Google': [1161], '14Wang': [1163], 'C.-Y.': [1164, 3862], 'Yang': [1165], 'He': [1167], 'X.': [1168, 1385, 3046], 'Chow': [1169], 'Du': [1171], 'Russell': [1173], 'J.T.': [1174], '32:': [1179, 1456], '99-112Google': [1180], '15Tsujimoto': [1182], 'Jeromin': [1184, 1375, 1398, 1822, 3034, 3059, 3117, 3863], 'Saitoh': [1186, 3119], 'N.': [1187, 3120], 'Roder': [1188, 1373, 1396, 1818, 3032, 3057, 3121, 3869], 'J.C.': [1189, 1476, 1488, 3122], 'Takahashi': [1190, 3123], 'Science.': [1192, 3125], '2276-2279Google': [1195, 3128], 'Scholar)': [1196, 1349, 1483, 1531, 1569, 1970, 3041, 3922, 3990, 4053, 4134, 4301], 'A-type': [1198], '(16Nakamura': [1201], 'T.Y.': [1202], 'Pountney': [1203], 'D.J.': [1204], 'Ozaita': [1205], 'Nandi': [1207], 'Ueda': [1209], 'Rudy': [1211], 'Coetzee': [1213], 'W.A.': [1214], '98:': [1223], '12808-12813Google': [1224], 'Despite': [1226], 'interactions,': [1228], 'known': [1238], 'certain,': [1240], 'thus': [1242, 1594, 4303], 'targets': [1246], 'remain': [1250], 'determined.': [1253], 'Recently,': [1254], 'yeast': [1255, 1271], 'demonstrated': [1258], 'genetically': [1260], 'biochemically': [1262], 'with,': [1264], 'regulate': [1266], 'of,': [1269], '(PI4K)': [1275], 'Pik1': [1276, 1336], '(17Hendricks': [1277], 'K.B.': [1278], 'B.Q.': [1280], 'Schnieders': [1281, 1318], 'E.A.': [1282, 1319, 4290], 'Thorner': [1283, 1320], 'Nat.': [1285, 1309, 1868, 3150], 'Cell.': [1286, 1310, 1869, 3151, 3387], '1:': [1289, 1313, 1872, 3154], '234-241Google': [1290], 'itself': [1293], 'essential': [1299, 1517], 'Golgi-to-cell': [1301], 'surface': [1302], 'trafficking': [1304], '(18Walch-Solimena': [1305], 'Novick': [1307], '523-525Google': [1314], '19Hama': [1316], 'Takemoto': [1322], 'J.Y.': [1323], 'DeWald': [1324], 'D.B.': [1325], '274:': [1330, 1968, 4252, 4299], '34294-34300Google': [1331], 'Similarly,': [1333], 'PI4Kβ': [1338, 1411, 3803, 3844, 3883, 4106, 4317], '(20Meyers': [1339], 'Cantley': [1341, 3671], 'L.C.': [1342, 3672], '272:': [1347], '4384-4390Google': [1348], 'if': [1360], 'immunoprecipitated': [1361], 'co-expression': [1363, 2899, 2955], 'non-neuronal': [1365], '(21Weisz': [1367], 'Gibson': [1369], 'G.A.': [1370], 'Leung': [1371], 'S.-M.': [1372], '24341-24347Google': [1382], '22Zhao': [1384], 'Varnai': [1386, 3047], 'Tuymetova': [1388, 3049, 3646], 'G.': [1389, 1453, 1858, 3050, 3140, 3647, 4044, 4288], 'Balla': [1390, 1400, 1553, 3051, 3061, 3260, 3652], 'Toth': [1392, 3053], 'Z.E.': [1393, 3054], 'Oker-Blom': [1394, 3055], '40183-40189Google': [1407, 3068], 'evolutionarily': [1415], 'conserved': [1416], 'functionally': [1418], 'important': [1419, 1430, 1572, 1621], 'NCS-1/frequenin.': [1423], 'Inositol': [1424], 'phospholipids': [1425], 'recently': [1427], 'emerged': [1428], 'regulators': [1431], 'exo-': [1433], 'endocytosis': [1435], '(23Cremona': [1436], 'Camilli': [1439, 3968], '114:': [1445], '1041-1052Google': [1446], '24Osborne': [1448], 'S.L.': [1449], 'Meunier': [1450], 'F.A.': [1451], 'Schiavo': [1452], '9-12Google': [1457], 'Regulated': [1459], 'dense': [1462, 1512], 'core': [1463, 1513], 'vesicles': [1464, 3896, 3957, 4159], 'require': [1469], 'transfer': [1472], '(PITPα)': [1474], '(25Hay': [1475], 'Martin': [1477, 1499, 1680], 'T.F.': [1478, 1645], '366:': [1481], '572-575Google': [1482], 'PI(4)P-5-kinase': [1486], '(26Hay': [1487], 'Fisette': [1489], 'P.L.': [1490], 'Jenkins': [1491], 'G.H.': [1492], 'Fukammi': [1493], 'K.': [1494, 3378, 3866, 4262, 4273], 'Takenawa': [1495], 'Anderson': [1497], 'R.A.': [1498, 4121], 'T.F.J.': [1500, 1681], '374:': [1503], '173-177Google': [1504], 'presence': [1509], '(27Wiedemann': [1520, 3897], 'Schaefer': [1522, 3899, 3911], 'Burger': [1524, 3901, 3913], 'M.M.': [1525, 3902, 3914], 'EMBO': [1526, 3903, 3972], '15:': [1529, 3906], '2094-2101Google': [1530, 3907], 'hypothesis': [1536], 'generation': [1538], 'PtdIns(4,5)P2': [1540, 1576, 1591, 1614, 1660, 1749, 1789, 3257], 'plasma': [1543, 1752, 4002, 4369], '(28Holz': [1545], 'Hlubek': [1547], 'M.D.': [1548, 4125], 'Sorensen': [1549], 'S.D.': [1550], 'Fisher': [1551], 'S.K.': [1552], 'Ozaki': [1555], 'Prestwich': [1557, 1678], 'G.D.': [1558, 1679], 'Stuenkel': [1559], 'E.L.': [1560], 'Bittner': [1561], 'M.A.': [1562, 1867, 3149], '17878-17885Google': [1568], 'step': [1573], 'secretion.': [1575, 1797, 4204, 4319], 'substrate': [1579], 'C': [1582], '(PLC)': [1583], 'activated': [1584], 'G-protein-coupled': [1586, 1604], 'changes': [1589, 1599], 'anticipated': [1595], 'lead': [1597], 'receptor': [1605], 'agonists.': [1606], 'play': [1619], 'PLC-mediated': [1628], 'cleavage': [1629], '(29Eberhard': [1630], 'Cooper': [1632], 'C.L.': [1633, 3662], 'Low': [1634], 'M.G.': [1635], 'Holz': [1636, 4115, 4126, 4293], 'R.W.': [1637, 4127, 4294], '1990;': [1640, 3676], '268:': [1641, 4132], '15-25Google': [1642], '30Martin': [1644], 'Loyet': [1646], 'K.M.': [1647, 1671], 'Barry': [1648], 'V.A.': [1649, 4372], 'Kowalchyk': [1650, 1672], 'Soc.': [1653], 'Trans.': [1654], '25:': [1656], '1137-1141Google': [1657], 'likely': [1662], 'act': [1664], 'directly': [1665, 3591], 'specific': [1668], '(31Loyet': [1670], 'Chaudhary': [1674], 'Chen': [1676, 3375], '8337-8343Google': [1687], 'study,': [1692], 'we': [1693, 4109], 'provide': [1694], 'evidence': [1695, 4219], 'through': [1703, 1783, 3578], 'regulating': [1704], 'PI4K.': [1708], 'myristoylation': [1716], 'mutant,': [1717], 'ATP-': [1723], 'ionomycin-evoked': [1725], 'expression': [1728, 3356], 'decreased': [1730, 2111], 'sensitivity': [1732], 'oxide': [1738, 2715], '(PAO),': [1739], 'membrane.': [1753], 'Finally,': [1754], 'mutations': [1756], 'interfere': [1763], 'exocytosis,': [1778, 4108], 'acts': [1782], 'activating': [1784], 'regulated': [1796, 4162], 'The': [1798, 1830, 1875, 1937, 2055, 2338, 2512, 2930, 2952, 3021, 3102, 3583, 3614, 3717, 3756, 4074], 'construct': [1800, 3248], 'generated': [1802, 1891, 1924], 'described': [1804, 1846, 1957, 2477, 2551, 2701, 2741, 3113, 3280, 3642, 3857], 'previously': [1805, 1847, 3114, 3643], '(32Chen': [1806], 'X.-L.': [1807], 'Zhong': [1808], 'Z.-G.': [1809], 'Yokoyama': [1810], 'Bark': [1812], 'Meister': [1814], 'Berggren': [1816], 'P.-O.': [1817], 'Higashida': [1820], 'Physiol.': [1825], '532:': [1827], '649-659Google': [1828], 'PI4Kβ,': [1831, 2750, 3810, 4086], 'PI4Kβ(D656A),': [1832], 'PI4KβN': [1834], 'terminus-GFP': [1835], 'constructs': [1836, 1879, 1941, 3015], 'kindly': [1838], 'provided': [1839], 'Rachel': [1841], 'Meyers': [1842, 1852, 3134], '(33Godi': [1848], 'Pertile': [1850, 3132], 'Marra': [1854, 3136], 'Di': [1856, 3138], 'Tullio': [1857, 3139], 'Iurisci': [1859, 3141], 'Luini': [1861, 3143], 'Corda': [1863, 3145], 'Matteis': [1866, 3148], '280-287Google': [1873, 3155], 'INP51-GFP': [1876], 'PH-EGFP/PH(R40L)-EGFP': [1878], 'donated': [1881], 'John': [1883], 'York': [1884, 3381], 'Tamas': [1886], 'Balla,': [1887], 'PCMV5-hGH': [1889], 'subcloning': [1893], 'coding': [1895], 'region': [1896], 'amplified': [1899], 'PCR': [1901], 'incorporating': [1903], '5′': [1904], 'EcoRI': [1905], '3′XbaI': [1907], 'sites,': [1908], 'sites': [1912], 'pCMV5.': [1914], 'mutants': [1916], '(G2A,': [1917], 'E120Q,': [1918], 'F22A,': [1919], 'E26A,': [1920], 'F55A,': [1921], 'T92A)': [1922], 'QuikChange': [1927], 'site-directed': [1928], 'mutagenesis': [1929], 'kit': [1930, 2426], '(Stratagene)': [1931], 'according': [1932, 2080, 2430], "manufacturer's": [1935, 2083, 2433], 'instructions.': [1936, 2434], 'identity': [1938], 'verified': [1943], 'automated': [1945], 'DNA': [1946], 'sequencing.': [1947], 'culture,': [1949], 'transfection,': [1950, 3285], 'experiments': [1953, 2179, 2436, 3290], 'performed': [1955, 2181, 2438, 2456], '(34Sugita': [1958, 4242], 'Janz': [1960, 4244], 'Sudhof': [1962, 4246, 4263, 4276], 'T.C.': [1963, 4247, 4264, 4277], '18893-18901Google': [1969, 4253], 'modifications.': [1973], '(Riken': [1976], 'Bank)': [1978], 'cultured': [1980], '100-mm': [1982, 2510, 3514], 'dishes': [1983, 2051, 2307, 2342, 3515], 'coated': [1984, 2757], 'collagen': [1986, 1989], '(rat': [1987], 'tail': [1988], 'type': [1990], 'I;': [1991], 'BD': [1992], 'Biosciences).': [1993, 2604, 2686], 'Cells': [1994, 2157, 2288, 2368, 2687, 2703, 2793, 2812, 2862, 3193, 3235, 3269, 3351, 3419, 3509, 3554], 'grown': [1996, 2482, 2772], '37': [1998, 2383, 2484, 2731, 2800], '°C': [1999, 2384, 2485, 2732, 2801], '5%': [2001, 2014, 2487, 2734], 'CO2': [2002, 2488], 'full': [2004, 2127, 2778, 3470], 'medium': [2005, 2089, 2492, 2496, 2709, 2779, 2785, 3414], '(RPMI': [2006], '1640': [2007, 2708, 3413], 'heat-inactivated': [2009], '10%': [2010, 2498], 'horse': [2011, 3160], 'serum': [2012, 2091, 2831], 'fetal': [2015, 2499], 'bovine': [2016, 2500], 'serum,': [2017, 2501], 'penicillin': [2018, 2502], '(50': [2019, 2023, 2503, 2507], 'units/ml),': [2020, 2504], 'streptomycin': [2022, 2506], 'units/ml)).': [2024], 'Confluent': [2025], '(passage': [2027], '6': [2028], 'throughout': [2029], 'experiments)': [2031], 'harvested': [2033, 2140], '0.125%': [2035], 'trypsin,': [2036], '0.5': [2037, 2201, 2217, 2346, 3536, 3702], 'mm': [2038, 2209, 2212, 2215, 2218, 2221, 2224, 2261, 2265, 2354, 2619, 2622, 3568, 3703], 'EDTA': [2039, 2355], 'PBS': [2041, 2807, 2820, 2870], 'without': [2042, 2090], 'Mg2+': [2045], 'plated': [2047, 2466, 2754, 3511], 'onto': [2048, 2467, 2755, 2917, 3512], 'collagen-coated': [2049, 2468, 3513], '12-well': [2050], '80%': [2053, 2474], 'confluency.': [2054], 'next': [2056, 2144, 2513], 'day': [2057, 2145, 2514, 2554], '(90–95%': [2058, 2176, 2515], 'confluency),': [2059, 2177, 2516, 3525], '0.6': [2060, 2065], 'μg': [2061, 2066, 2526, 2538, 3426, 3430, 3436], 'pCMV5-hGH': [2063, 2692, 2697], 'plasmid': [2068], 'interest': [2070, 4145, 4201], 'co-transfected': [2072, 2689, 3237, 4148, 4401], '4': [2074, 2119, 2651, 3612], 'μl': [2075, 2561, 3559, 3700, 3732, 3751], 'LipofectAMINE': [2077, 2099, 2547], '2000': [2078, 2100, 2548], '(Invitrogen)': [2079, 2549], 'specification': [2084], "Dulbecco's": [2086], 'modified': [2087, 2494], "Eagle's": [2088, 2495], 'antibiotics': [2093], 'efficiency.': [2097], 'Each': [2098], 'tube': [2101], 'once,': [2105, 3473], 'co-transfection': [2109, 2155, 3017, 4181, 4211], 'efficiencies': [2110, 2985, 3006, 3018], 'when': [2112, 4065], 'tubes': [2113], 're-used': [2115], 'multiple': [2116], 'times.': [2117], 'After': [2118, 2653], 'h,': [2120], 'mixture': [2123, 3739], 'replaced': [2125], 'medium.': [2128, 2367], 'two': [2132, 2167, 4183, 4333], 'wells': [2133], 'transfected': [2134, 2195, 2519, 4153], 'same': [2137, 2192, 4188], 'plasmids': [2138, 4137, 4185], 'pooled': [2142], 'compensate': [2147], 'possible': [2149, 4192], 'slight': [2150], 'differences': [2151], 'efficiencies.': [2156], 're-plated': [2159], '24-well': [2161], 'plates': [2162, 2472], 'ratio': [2165], '12-wells': [2168], '24-wells.': [2171], 'Two': [2172, 3521], 'days': [2173, 2775, 2927, 3283, 3502, 3522], 'after': [2174, 2928, 3275, 3284, 3503], 'replating': [2175], 'test': [2184], 'conditions': [2187, 2722, 4015], 'carried': [2188], 'out': [2189], 'pool': [2193], 'Controls': [2197], 'treated': [2199], 'ml': [2202, 2347, 3468], 'solution': [2206, 2257], '(PSS;': [2207], '145': [2208], 'NaCl,': [2210, 2620, 3569], '5.6': [2211, 2220], 'KCl,': [2213], '2.2': [2214], 'CaCl2,': [2216], 'MgCl2,': [2219], 'glucose,': [2222], '15': [2223, 2728, 3425, 3429, 3435, 3609], 'Hepes-NaOH,': [2225], 'pH': [2226, 2624, 3565], '7.4).': [2227], 'stimulated': [2231, 2248, 2271], '10': [2234, 2299, 2320, 2537, 2576, 3467, 3570, 3706, 3722], 'min': [2235, 2321, 2729, 2798, 2823, 2873, 3441, 3610, 3723], 'incubation': [2236, 2252, 2275, 4066], 'ATP': [2238, 3704, 4321, 4393], 'buffer': [2239, 2828, 3562, 3631, 3694], '(PSS': [2240, 2258], 'containing': [2241, 2259, 2352, 2710, 2786, 2808, 3705], '300': [2242], 'μm': [2243], 'ATP),': [2244], 'KCl-triggered': [2245], '10-min': [2251, 2274], 'high': [2254, 4178], '95': [2260], 'NaCl': [2262], '56': [2264], 'KCl),': [2266], 'ionomycin-triggered': [2268], 'ionomycin': [2277], '(5': [2278], 'μmionomycin': [2279], '(Sigma)': [2280, 2716], 'final': [2283], 'Me2SO': [2284, 2718], 'concentration': [2285, 2579, 2719], '0.5%': [2286, 2725, 2816, 2835, 2866], '(v/v)).': [2287], 'incubated': [2290, 2659, 2705, 2875, 3423, 3720], 'α-latrotoxin': [2292], '(final': [2293, 2717], '1.5': [2294], 'nm)': [2295], 'PSS': [2297], 'min.': [2300, 2577], 'At': [2301], 'end': [2303], 'experiment,': [2306], 'transferred': [2309, 2599, 3465], 'ice,': [2311], 'supernatant': [2314, 2329, 2399, 2582, 3615], 'removed': [2316], 'centrifuged': [2318, 2570], 'Eppendorf': [2324, 2363, 2394, 3543], 'centrifuge.': [2325, 2395], 'hGH': [2326, 2396, 2408, 2413, 2449, 2966, 4139, 4155, 4203, 4208, 4396], 'centrifugation': [2332, 2364, 2402, 3603, 3618], 'taken': [2334, 2404, 3620, 3633, 4217], 'secreted': [2336], 'hGH.': [2337], 'resuspended': [2344, 3410, 3556, 3628], 'ice-cold': [2349], '1': [2353, 2627, 2672, 2762, 2841, 2857, 2880, 3083, 3734, 3814, 3997, 4026], 'added': [2357], 'pellet': [2360, 3626], 'lysed': [2371, 2558], 'five': [2373, 3576], 'freeze-thaw': [2374], 'cycles': [2375], '(in': [2376], 'dry': [2378, 2611], 'ice/ethanol': [2379], 'bath': [2380], 'heating': [2385], 'block),': [2386], 'insoluble': [2388], 'material': [2389], 'pelleted': [2391, 3541], 'cellular': [2407], 'secreted.': [2412], 'samples': [2418], 'measured': [2420, 3492, 3640], 'enzyme-linked': [2423], 'immunosorbent': [2424], 'assay': [2425, 2587, 4112, 4229], '(Roche': [2427], 'Molecular': [2428], 'Biochemicals)': [2429], 'duplicates': [2440], 'or': [2441, 2641, 2695, 2781, 2979, 3042, 3167, 3178, 3216, 3225, 3240, 3250, 3274, 3433, 3595], 'triplicates,': [2442], 'average': [2445], 'percent': [2446], 'total': [2448, 2592, 3342], 'released': [2450], 'calculated.': [2452], 'Statistical': [2453], 'analyses': [2454], 'paired': [2459, 3404], "Student's": [2460], 't': [2461], 'test.': [2462, 3406], '6-well': [2469, 2531], 'tissue': [2470], 'culture': [2471], 'confluency': [2475], 'above.': [2478, 2552, 2702, 2742], 'COS-7': [2479, 2543], 'COS': [2490], "(Dulbecco's": [2493], 'units/ml))': [2508], 'dishes.': [2511], 'appropriate': [2522], 'vector': [2523], '2.4': [2525], 'vector/well': [2528], 'plate': [2532], 'vector/100-mm': [2540], 'dish': [2541], 'respectively,': [2545, 2645], 'One': [2553], 'later,': [2555], '500': [2560], 'hot': [2563], '1%': [2564], 'SDS,': [2565], 'lysates': [2568], '12,000': [2572], '×': [2573, 3309, 3416, 3518, 3537, 3606], 'g': [2574, 3607], 'Protein': [2578], 'determined': [2584], 'BCA': [2586], '(Pierce).': [2588], 'Equal': [2589], 'amounts': [2590], 'separated': [2595, 3317], 'SDS-PAGE': [2597], 'Hybond-N': [2601], '(Amersham': [2603, 2685], 'Membranes': [2605], 'blocked': [2607], '2%': [2609], 'non-fat': [2610], 'milk': [2612], 'TTBS': [2614], '(0.05%': [2615], 'Tween': [2616], '20,': [2617], '150': [2618], '20': [2621, 2797, 3446], 'Tris,': [2623], '7.5)': [2625], 'h': [2628, 2673, 2763, 2842, 2858, 2881], 'room': [2630, 2675, 2844, 2860, 2883, 3725], 'temperature': [2631, 3726], 'hybridized': [2633], 'primary': [2635, 2850, 4068], 'antibody': [2636, 2665, 2851, 2939], '(either': [2637], 'anti-NCS-1': [2638, 3026, 3107], 'rabbit': [2639, 2643, 3027, 3071, 3090, 3185], 'polyclonal': [2640, 3028, 3044, 3072, 3091, 3106], 'anti-PI4Kβ': [2642, 3070, 3109], 'polyclonal,': [2644], '1:1000': [2647], 'dilution)': [2648], 'overnight': [2649], '°C.': [2652, 3613], 'washing': [2654], 'TTBS,': [2656], 'blots': [2657], 'horseradish': [2662], 'peroxidase-tagged': [2663], 'anti-rabbit': [2664, 3172], '(DAKO': [2666], 'A/S)': [2667], '1:2000': [2669], 'dilution': [2670], 'temperature,': [2676, 2845], 'washed,': [2677], 'developed': [2679, 4113], 'chemiluminescence': [2683], 'system': [2684, 4309], 'either': [2691, 2777, 2977, 2995, 3195, 3243, 3271], 'pcDNA3.1': [2694], 'pcDNA3.1-NCS-1,': [2699], 'RPMI': [2707, 3412], 'concentrations': [2712], 'maintained': [2723], '(v/v))': [2726], 'CO2,': [2735], 'followed': [2736, 2846], 'assays': [2739], 'To': [2743, 2885, 3303, 3795, 4099], 'visualize': [2744], 'endogenous': [2747, 2944, 3828], 'non-transfected': [2751, 3819], 'coverslips': [2756, 2919], 'poly-l-lysine': [2759], '(coated': [2760], '50': [2765, 2787], 'μg/ml': [2766], 'poly-l-lysine,': [2767], 'M': [2768], 'r30,000–70,000': [2769], '(Sigma))': [2770], '3': [2774, 3440], '(non-differentiated)': [2780], 'serum-reduced': [2783], '(1%)': [2784], 'ng/ml': [2788], 'NGF': [2789, 3881, 4010], '2.5': [2790], 'S': [2791], '(Invitrogen).': [2792], 'fixed': [2795, 2921, 3299], '4%': [2803, 2809], '(w/v)': [2804, 2810], 'paraformaldehyde': [2805], 'sucrose.': [2811], 'permeabilized': [2814], 'Triton': [2817, 2836, 2867], 'X-100': [2818, 2837, 2868], '30': [2822, 2872], 'preincubated': [2825], 'blocking': [2827, 2854], '(10%': [2829], 'goat': [2830, 3171], '(Vector': [2832], 'Laboratories)': [2833, 3183], 'PBS)': [2839], '(diluted': [2852], 'buffer)': [2855], 'temperature.': [2861, 2884], 'washed': [2864, 3472, 3530], 'secondary': [2877], 'antibodies': [2878, 3023, 3110, 3158, 3807, 4069], 'determine': [2886], 'amount': [2888, 2897, 2931, 2953], 'well': [2894, 3316], '50%': [2904], 'processed': [2908, 2923], 'assays,': [2911], 'remainder': [2914], 'replated': [2916], 'poly-l-lysine-coated': [2918], 'immunocytochemistry': [2925], '2': [2926, 3282, 3501], 'replating.': [2929], 'overexpressed': [2933, 2950], 'qualitatively': [2936], 'assessed': [2937], 'dilutions': [2940], 'barely': [2942], 'easily': [2948], 'detected': [2949], 'proteins.': [2951], 'calculated': [2957, 2987, 3337], 'counting': [2959, 2989, 3494], 'number': [2961, 2991], 'expressing': [2964, 2976, 2994, 3499], 'interest,': [2971], 'compared': [2973], 'one': [2978], 'only.': [2983], 'Transfection': [2984, 3005], '700–1200': [2998], 'counted': [3001, 3347], 'each': [3003, 3314, 3322, 3334, 3349], 'regularly': [3007], 'amounted': [3008, 3484, 3505], 'around': [3010, 3486, 3507], '15%,': [3011], 'reached': [3016], '≥90%.': [3020], 'following': [3022], 'used:': [3025], '(44162)': [3029], '(35Werle': [3030], 'M.J.': [3031, 4385], 'Neurosci.': [3036, 3874, 3918], 'Lett.': [3037], '284:': [3039], '33-36Google': [3040], 'chicken': [3043], '(22Zhao': [3045], '(Upstate': [3073], 'Biotechnology),': [3074], 'anti-synaptobrevin/VAMP2': [3075], 'mouse': [3076, 3084], 'monoclonal': [3077, 3085], '(Cl': [3078], '69.1;': [3079], 'Synaptic': [3080], 'Systems),': [3081], 'anti-syntaxin': [3082], '(HPC-1;': [3086], 'Sigma),': [3087], 'anti-hGH': [3089], '(National': [3092], 'Hormone': [3093], 'Peptide': [3095], 'Program,': [3096], 'NIDDK,': [3097], 'National': [3098], 'Institutes': [3099], 'Health).': [3101], 'specificities': [3103], '(15Tsujimoto': [3115], '33Godi': [3130], 'Secondary': [3157], 'included': [3159], 'anti-mouse': [3161], 'IgG': [3162, 3173, 3187], 'conjugated': [3163, 3174, 3188], 'fluorescein': [3165, 3176, 3190], 'isothiocyanate': [3166, 3177, 3191], 'Texas': [3168], 'red': [3169], '(TR),': [3170], 'TR': [3179], '(all': [3180], 'Vector': [3182], 'anti-chicken': [3186], '(Sigma).': [3192], 'examined': [3196], 'confocal': [3199], 'microscope': [3200, 3220], '(Leica': [3201], 'TCS': [3202], 'NT)': [3203], 'under': [3204, 3222, 4014], '×40': [3206, 3224, 3328], 'oil-immersion': [3207, 3227, 3329], 'objective,': [3208, 3228], 'acquired': [3212, 3232, 3325], 'Leica': [3214], 'software': [3215], 'upright': [3219], '(Leica)': [3221], '×100': [3226], 'IPLab.': [3234], 'NCS-1(G2A)': [3241], 'PH': [3246], 'domain-GFP': [3247], '(PH-EGFP)': [3249], 'PH(R40L)-EGFP,': [3251], 'mutant': [3253], 'unable': [3254], 'bind': [3256], '(36Varnai': [3258], '143:': [3266], '501-510Google': [3267], 'live': [3273], 'fixation': [3276], 'permeabilization': [3278], 'above,': [3281], 'identical': [3287], 'results.': [3288], 'measuring': [3291], 'PH-EGFP': [3292, 3355], 'intensities': [3293, 3332], 'PAO': [3295], 'treatment,': [3296], 'permeabilized.': [3302], 'quantify': [3304], 'intensities,': [3305], 'rectangles': [3306], '80': [3308], '110': [3310], 'pixels': [3311], 'centered': [3312], 'GFP-positive,': [3315], 'collected': [3320], 'random': [3323], 'field': [3324], 'objective.': [3330], 'Pixel': [3331], 'rectangle': [3335], 'IPLab,': [3339], '100': [3344, 3558, 3567], 'condition.': [3350], 'highest': [3354], 'excluded,': [3359], 'they': [3361], 'often': [3362], 'gained': [3363], 'rounded': [3365], 'appearance': [3366], 'formed': [3368], 'blebs': [3370], '(37Raucher': [3371], 'Stauffer': [3373], 'W.': [3376], 'Shen': [3377], 'Guo': [3379], 'J.D.': [3382], 'Sheetz': [3383], 'M.P.': [3384], 'Meyer': [3385], '100:': [3389], '221-228Google': [3390], 'Experiments': [3392], 'done': [3394], 'times,': [3396], 'analyzed': [3401, 3760], "Student'st": [3405], '(3': [3415], '107': [3417], 'cells/ml).': [3418], '(400': [3420], 'μl)': [3421], 'pcDCNA3.1-NCS-1,': [3428], 'pcDNA3.1,': [3432], 'pCMV5-GFP': [3438], 'electroporated': [3443], '(330': [3444], 'V,': [3445], 'ms)': [3447], 'ECM': [3450], '830': [3451], 'Square': [3452], 'Wave': [3453], 'Electroporator': [3454], '(BTX;': [3455], 'Genetronics': [3456], 'Inc.,': [3457], 'San': [3458], 'Diego,': [3459], 'CA).': [3460], 'Electroporated': [3461], 'immediately': [3464], 'medium,': [3471], 'counted.': [3475], 'Under': [3476], 'indicated': [3478], 'electroporation': [3479, 3489], 'conditions,': [3480], 'survival': [3482], 'usually': [3483], '10–15%,': [3487], 'efficiency,': [3490], 'fraction': [3496], 'GFP': [3500], 'electroporation,': [3504], '50%.': [3508], '5': [3517, 3699], '106': [3519, 3538], 'cells/dish.': [3520], 'later': [3523], '(95%': [3524], 'attached': [3526], 'harvested,': [3529], 'counted,': [3534], 'tubes.': [3544], 'Pellets': [3545], 'quick-frozen': [3547], 'liquid': [3549], 'N2': [3550], 'until': [3551], 'further': [3552, 3600], 'use.': [3553], '(20': [3563], 'mmHepes/NaOH,': [3564], '7.5,': [3566], 'mmMgCl2)': [3571], 'disrupted': [3573], 'passing': [3575], 'times': [3577], '17-gauge': [3580], 'needle': [3581], 'syringe.': [3582], 'aliquot': [3584], 'prepared': [3587], 'lysate': [3588, 3598, 3686], 'assay,': [3594], 'fractionated': [3601], '10,000': [3605], 'fraction,': [3623], 'fraction.': [3636], '(61Balla': [3644], 'Barshishat': [3648], 'Geiszt': [3650], '20041-20050Google': [3659], '62Carpenter': [3661], 'Duckwoth': [3663], 'B.C.': [3664], 'Auger': [3665], 'K.R.': [3666], 'Cohen': [3667], 'Schaffhausen': [3669], 'B.S.': [3670], '265:': [3677], '19704-19711Google': [3678], 'Briefly,': [3680], 'initiate': [3682], 'phospholipid': [3683], 'fractions': [3689], 'diluted': [3691], '45': [3696], 'μl,': [3697], 'μCi': [3707], '[γ-32P]ATP': [3709], '(3000–6000': [3710], 'Ci/mmol;': [3711], 'PerkinElmer': [3712], 'Life': [3713], 'Sciences)': [3714], 'added.': [3716], 'reaction': [3718], 'stopped': [3728], 'adding': [3730, 3749], '60': [3731, 3768], 'n': [3735], 'HCl.': [3736], 'two-phase': [3738], 'induced,': [3741], 'phosphorylated': [3744], 'lipids': [3745], 'extracted': [3747, 3757], '160': [3750], 'chloroform:methanol,': [3753], '1:1': [3754], '(v/v).': [3755], 'products': [3758], 'thin': [3762, 3769], 'layer': [3763, 3770], 'chromatography': [3764], '(TLC)': [3765], '(silica': [3766], 'gel': [3767], 'plates,': [3771], 'chloroform/methanol/15n': [3773], 'ammonium': [3774], 'hydroxide/water': [3775], '(90:90:7:22)': [3776], '(v/v/v/v)': [3777], 'solvent': [3780], 'system).': [3781], 'Radiolabeled': [3782], 'PtdIns(4)P': [3783, 3793], 'TLC': [3787], 'mobility': [3788], 'comparison': [3790], 'purified': [3792], 'standard.': [3794], 'identify': [3796], 'subcellular': [3798, 4076, 4081], 'against': [3808], 'VAMP2,': [3811], 'syntaxin': [3813, 4025], 'stain': [3818], 'non-differentiated': [3824, 3939], 'NGF-differentiated': [3826], 'mainly': [3831], 'diffuse': [3832], 'cytosolic,': [3834], 'additional': [3837], 'perinuclear': [3838, 3854, 3936, 4097], 'staining': [3839, 3845, 3884, 3930, 4007], '(Fig.1,': [3840], 'B).': [3843], 'observed': [3847, 3933, 4008, 4064], 'area,': [3855], '(38Pan': [3861], 'Lundstrom': [3865], 'Yoo': [3867], 'S.H.': [3868], 'Fox': [3871], 'A.P.': [3872], '222:': [3876], '2427-2433Google': [3877], 'differentiation,': [3882, 4011], 'enriched': [3886, 3944], 'processes,': [3888], 'consistent': [3889], 'secretory': [3895, 3956, 4163], '39Wiedemann': [3909], 'Sihra': [3915], 'T.S.': [3916], '18:': [3920], '5594-5602Google': [3921], '(Fig.': [3923, 3991, 4054], '1,C': [3924], 'D).': [3926], 'comparison,': [3928], 'VAMP2': [3929], 'primarily': [3932], 'area': [3937], 'significantly': [3943], 'processes': [3946], 'differentiated': [3948], 'accordance': [3951], '(40Baumert': [3961], 'Maycox': [3963], 'P.R.': [3964], 'Navone': [3965], 'Jahn': [3970, 4045], '1989;': [3974], '8:': [3975], '379-384Google': [3976], '41Papini': [3978], 'Rossetto': [3980], 'Cutler': [3982], 'D.F.': [3983], '270:': [3988], '1332-1336Google': [3989], '1,': [3992], 'E': [3993], 'F).': [3995], 'Syntaxin': [3996], 'localized': [3999], 'membrane,': [4003], 'intense': [4017], 'fusion,': [4019], 'occurs': [4021], 'during': [4022], 'neurite': [4023], 'formation,': [4024], 'undergoes': [4027], 'recycling': [4028], 'differentiating': [4030], '(42Walch-Solimena': [4033], 'Blasi': [4035], 'Edelmann': [4037], 'Chapman': [4039], 'E.R.': [4040], 'Fischer': [4041], 'von': [4042], 'Mollard': [4043], '128:': [4051], '637-645Google': [4052], '1,G': [4055], 'H).': [4057], 'cases,': [4060], 'immunostaining': [4062], 'omitted': [4071], '(not': [4072], 'shown).': [4073], 'comparative': [4075], 'similar': [4080], 'distributions': [4082], 'being': [4090], 'partially': [4091, 4094], 'cytosolic': [4092], 'associated': [4095], 'structures.': [4098], 'study': [4100, 4311], 'employed': [4110], 'co-workers': [4117], '(43Wick': [4118], 'P.F.': [4119], 'Senter': [4120], 'Parsels': [4122], 'L.A.': [4123], 'Uhler': [4124], '10983-10989Google': [4133], 'encoding': [4138], 'second': [4142], 'transiently': [4147], 'packaged': [4157], 'pathway': [4164], 'serves': [4166, 4304], 'reporter': [4169], 'stimulation.': [4176], 'probability': [4179], 'distinct': [4184], 'makes': [4190], 'it': [4191], 'investigate': [4194], 'change': [4206], 'particular': [4214], 'involvement': [4222], 'successfully': [4232], 'implicate': [4235], '44Wang': [4255], 'Y.': [4256], 'Okamoto': [4257], 'Schmitz': [4259], 'Hofmann': [4261], '388:': [4267], '593-598Google': [4268], '45Sugita': [4270], 'Ichtchenko': [4272], 'Khvotchev': [4274], '32715-32724Google': [4283], '46Chung': 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