Title: Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea
Abstract: Article15 January 2003free access Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea Andrew J. Foster Andrew J. Foster Present address: The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH UK Search for more papers by this author Joanna M. Jenkinson Joanna M. Jenkinson School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG UK Search for more papers by this author Nicholas J. Talbot Corresponding Author Nicholas J. Talbot School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG UK Search for more papers by this author Andrew J. Foster Andrew J. Foster Present address: The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH UK Search for more papers by this author Joanna M. Jenkinson Joanna M. Jenkinson School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG UK Search for more papers by this author Nicholas J. Talbot Corresponding Author Nicholas J. Talbot School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG UK Search for more papers by this author Author Information Andrew J. Foster2, Joanna M. Jenkinson1 and Nicholas J. Talbot 1 1School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG UK 2Present address: The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH UK *Corresponding author. E-mail: [email protected] The EMBO Journal (2003)22:225-235https://doi.org/10.1093/emboj/cdg018 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The relationship of trehalose metabolism to fungal virulence was explored in the rice blast fungus Magnaporthe grisea. To determine the role of trehalose synthesis in pathogenesis, we identified and deleted TPS1, encoding trehalose-6-phosphate synthase. A Δtps1 mutant failed to synthesize trehalose, sporulated poorly and was greatly attenuated in pathogenicity. Appressoria produced by Δtps1 did not develop full turgor or elaborate penetration hyphae efficiently. To determine the role of subsequent trehalose breakdown, we deleted NTH1, which encodes a neutral trehalase. Nth1 mutants infected plants normally, but showed attenuated pathogenicity due to a decreased ability to colonize plant tissue. A second trehalase was also identified, required both for growth on trehalose and mobilization of intracellular trehalose during infection-related development. TRE1 encodes a cell wall-localized enzyme with characteristics of both neutral and acidic trehalases, but is dispensable for pathogenicity. Our results indicate that trehalose synthesis, but not its subsequent breakdown, is required for primary plant infection by M.grisea, while trehalose degradation is important for efficient development of the fungus in plant tissue following initial infection. Introduction Trehalose (α-D-glucopyranosyl-α-D-glucopyranoside) is a non-reducing disaccharide that is commonly found as a storage carbohydrate in eukaryotic cells (Arguelles, 2000) and implicated in the response to various environmental stresses (Thevelein, 1984, 1996). In Saccharomyces cerevisiae, trehalose has been shown to be required by cells to contend with diverse stresses such as heat shock, starvation and desiccation (De Virgilio et al., 1994). Trehalose is also known to protect enzymes from thermal stress in vitro, providing a potential means for cells to survive during adverse conditions (Hottiger et al., 1994). Investigations to determine the biological function of trehalose in eukaryotes have been limited predominantly to the study of free-living species. Consequently, little is known about trehalose metabolism in pathogenic microorganisms. Many virulence-associated functions, including infection-related development and colonization of host tissue, require pathogens to respond to rapid changes in external environment and to mobilize storage carbohydrates. In this study, we set out to determine the role of trehalose metabolism in a plant pathogenic fungus, Magnaporthe grisea. This pathogen is the causal agent of rice blast disease, the most serious disease of cultivated rice (Talbot and Foster, 2001). Trehalose metabolism in eukaryotic cells has been studied in most detail in S.cerevisiae. Here, trehalose is synthesized by a multienzyme complex containing trehalose-6-phosphate synthase (T6PS) encoded by TPS1, a trehalose-6-phosphatase encoded by TPS2, and two regulatory subunits encoded by the TSL1 and TPS3 genes (Vuorio et al., 1993; Bell et al., 1998). Trehalose-6-phosphate (T6P) is synthesized using UDP-glucose and glucose-6-phosphate as substrates, and then directly converted to trehalose. Three distinct trehalases are present in S.cerevisiae. Acidic trehalase, encoded by ATH1, is required for utilization of trehalose as a carbon source and is a vacuolar enzyme with optimum activity at low pH (Londesborough and Varimo, 1984; Mittenbühler and Holzer, 1988). Mobilization of intracellular trehalose is catalysed by cytoplasmic neutral trehalase, encoded by NTH1 and NTH2 (Nwaka and Holzer, 1998). In filamentous fungi such as Aspergillus nidulans, trehalose synthesis involves T6PS encoded by the tpsA gene (Fillinger et al., 2001), and trehalose metabolism involves an acidic, cell wall-localized trehalase required for utilization of exogenous trehalose (d‘Enfert and Fontaine, 1997), and a cAMP-regulated neutral trehalase (Nth1) required for trehalose mobilization during spore germination (d'Enfert et al., 1999). We reasoned that several virulence-associated functions in M.grisea might involve trehalose mobilization, e.g. germination of conidia and the development of infected cells on the leaf surface, and subsequent plant tissue colonization. Here we report the isolation and characterization of genes encoding biosynthetic and degradative enzymes involved in trehalose metabolism in M.grisea. We show that trehalose synthesis in M.grisea is mediated by a T6PS-encoding gene, TPS1, which is required for production of functional infection structures and primary plant infection. We also demonstrate that trehalose breakdown involves two trehalases; a neutral trehalase, encoded by a gene NTH1, which is important for invasive growth in plant cells, and a novel trehalase encoded by TRE1 which is required for trehalose mobilization during spore germination, but dispensable for pathogenicity. Taken together, our results indicate that trehalose synthesis is required for appressorium-mediated plant infection by a plant pathogenic fungus, while mobilization of stored trehalose is significant only after cuticle penetration. Results Trehalose synthesis is required for pathogenicity of M.grisea To determine the role of trehalose synthesis in pathogenesis of M.grisea, we identified a gene encoding T6PS by designing degenerate primers to regions conserved among T6PS genes. A 650 bp product was amplified and used to identify a cDNA and genomic clone. This revealed the presence of a 1587 bp open reading frame (ORF) capable of encoding a 529 amino acid protein of 58.4 kDa. We named the gene TPS1, and the putative gene product showed 76% identity to tpsA from A.niger and 65% identity to TPS1 from S.cerevisiae (DDBJ/EMBL/GenBank accession No. AY148093; see Supplementary data available at The EMBO Journal Online). A gene replacement of TPS1 was carried out. For this, an 8 kb Pst I fragment was isolated and a 2.0 kb NcoI fragment containing part of the TPS1 promoter and most of the ORF was removed and replaced with the Hph cassette. The resulting vector was introduced into a wild-type rice pathogenic strain of M.grisea, Guy11, and 40 transformants were selected. In DNA gel blots, one transformant, T-5-13, had undergone the Δtps1 gene replacement (see Supplementary data). The putative Δtps1 mutant T-5-13 was selected and mycelial cultures prepared for measurement of intracellular trehalose. We found that trehalose was almost completely absent from the Δtps1 mutant, compared with levels observed in Guy11 and either Δnth1 or tre1::Hph mutants (Figure 1). Figure 1.Bar chart of trehalose levels in mycelium of M.grisea strains Guy11, Δtps1 mutant T-5-13, Δnth1 mutant T-2-8 and tre1::Hph mutant T-8-45. Data points represent the mean value from three experiments. The error bar represents the standard deviation. Download figure Download PowerPoint We investigated the role of TPS1 in growth, development and virulence of M.grisea. The Δtps1 mutant showed normal mycelial growth and did not respond differently when exposed to heat shock (not shown). Sporulation was dramatically reduced in the Δtps1 mutant. Guy11 produced a mean of 1.39 × 10 6 conidia per plate culture (n = 8) compared with 3.6 × 103 for Δtps1 strain T-5-13 (P < 0.001). Conidial germination rates and appressorium formation were not significantly affected by the Δtps1 mutation (not shown). To investigate the role of TPS1 in pathogenesis, conidial suspensions were sprayed onto seedlings of the susceptible rice cultivar CO-39. A consistent 90% reduction in disease lesion development was found in plants inoculated with Δtps1 compared with Guy11, as shown in Figure 2A (t-test, P < 0.001). The small number of disease lesions in Δtps1 infections did not expand during infection or produce sporulating hyphae. The Δtps1 mutant was also non-pathogenic on barley. The pathogenicity of Δtps1 mutants could not be restored by adding trehalose or T6P to conidia, prior to inoculation (not shown). Introduction of the TPS1 gene into Δtps1 mutant T-5-13 restored its ability to cause disease (Figure 2A). All other Δtps1 mutant phenotypes were complemented by introduction of TPS1 (not shown). We conclude that TPS1 is required for trehalose synthesis in M.grisea, and is necessary for rice blast disease. Figure 2.Phenotypic analysis of M.grisea Δtps1 mutant T-5-13. (A) Virulence phenotype of the Δtps1 mutant. Seedlings of cv. CO-39 were inoculated with M.grisea conidial suspensions of identical concentration (2 × 104 conidia/ml) of strain Guy11 (leaf 1), Δtps1 mutant T-5-13, and a transformant (T-10-4) of Δtps1mutant T-5-13 carrying a single copy insertion of pAJF200, an 8 kb PstI fragment of TPS1. Seedlings were incubated for 4 days for development of rice blast disease. (B) Growth of M.grisea strain Guy11, Δtps1 mutant T-5-13, Δnth1 mutant T-2-8 and tre1::Hph mutant T-8-45 with glucose as sole carbon source. (C) Appressorium turgor of Guy11 (grey diamonds) and Δtps1 mutant T-5-13 (black squares) was measured by incipient cytorrhysis. The proportion of appressoria that collapsed after exposure to solutions of between 0 and 5.0 M glycerol was recorded. Each data point is the mean of three independent observations. The error bar represents the standard deviation. Download figure Download PowerPoint TPS1 is required for appressorium-mediated cuticle penetration To determine why Δtps1 mutants are non-pathogenic, the ability of the Δtps1 mutant to penetrate intact rice cuticles was measured. Appressoria developed normally in the Δtps1 mutant, but production of penetration hyphae and rupture of the plant cuticle were significantly decreased from 81 ± 7.2% successful penetrations by Guy11 to 26.6 ± 17.4% in Δtps1 (P < 0.001). Consistent with this, the Δtps1 mutant did not cause rice blast symptoms when spores were inoculated on intact rice leaves, but was able to produce disease symptoms when the rice cuticle was first removed by abrasion (not shown). Taken together, the experiments indicate that TPS1 is required for plant infection, but not subsequent growth of the fungus in rice tissue. Plant infection by M.grisea is brought about by the appressorium, which develops very high internal turgor to facilitate generation of mechanical force to breach the rice leaf cuticle. Appressorium turgor can be measured using an incipient cytorrhysis assay which uses hyperosmotic concentrations of a solute to collapse appressoria, thereby allowing estimation of their internal solute concentration and turgor (Howard et al., 1991; de Jong et al., 1997). To carry out this assay, appressoria were allowed to form on a hydrophobic plastic surface and then incubated in glycerol solutions of varying concentration (Figure 2C). We found that 1.5 M glycerol was sufficient to collapse 21.8 ± 10.4% of appressoria of Guy11 and 53.4 ± 15.7% of Δtps1 appressoria, indicating a significant decrease in turgor in the Δtps1 mutant (P = 0.0074). Turgor was not restored by adding trehalose or T6P to germinating conidia of Δtps1 (not shown). We conclude that TPS1 is required for appressorium-mediated cuticle penetration by M.grisea. M.grisea TPS1 is required for growth on glucose In S.cerevisiae, T6PS is required for regulation of glucose intake into glycolysis (Thevelein and Hohmann, 1995). As a consequence, yeast Δtps1 mutants are unable to grow on glucose. Conversely, in the filamentous fungus A.nidulans, tpsA may play a role in regulating levels of sugar-phosphates, but mutants lacking T6PS still grow on glucose (Fillinger et al., 2001). To determine whether M.grisea TPS1 is required for glycolytic regulation, we incubated the Δtps1 mutant on minimal growth medium with glucose as sole carbon source (Figure 2B). The Δtps1 mutant was unable to grow on glucose or other simple sugars including fructose, sucrose, maltose and trehalose (Table I). In addition, unlike yeast T6PS mutants, M.grisea Δtps1 failed to grow on acetate or fatty acids, indicating that gluconeogenesis is also affected by the absence of the T6PS. In rich growth media, we observed that Δtps1 mutants were able to utilize glucose, but only in the presence of a rich source of amino acids, provided either from yeast extract, peptone or casamino acids. Under these conditions, Δtps1 mutants were still unable to utilize acetate as a carbon source (Table I). We conclude that M.grisea TPS1 is required for growth on a range of rapidly fermentable carbon sources, and may be required for regulation of glycolysis and gluconeogenesis. Table 1. Growth of trehalose metabolic mutants of M.grisea on different carbon sources and in supplemented growth medium Growth medium Guy-11 Δtps1 Δnth1 tre:Hph Complete medium (CM)a + + +b + + + + + + + + + Oatmeal agar + + + + + + + + + + CM – glucose − − − − CM – nitrate salts + + + + + + + + + + + + CM – peptone + + + + + + + + + + + + CM – yeast extract + + + + + + + + + + + + CM – casamino acids + + + + + + + + + + + + CM – vitamin solution + + + + + + + + + + + + CM – yeast extract and casamino acids + + + + + + + + + + + + CM – yeast extract and peptone + + + + + + + + + + + + CM – yeast extract and glucose − − − − Minimal medium (MM) + glucosec + + + − + + + + + + MM + trehalose + + + − + + + − MM + fructose + + + − + + + + + + MM + maltose + + + − + + + + + + MM + sucrose + + + − + + + + + + MM + olive oil + + − + + + + MM + sodium acetate + + − + + MM + sodium acetate + glucose + + + − + + + + + + MM + mannitol + + − + + MM + mannitol + glucose + + + − + + + + + + MM + galactose − − − − MM + glycerol − − − − MM + glycerol + glucose + + + − + + + + + + MM + ethanol − − − − MM + ethanol + glucose + + + − + + + + + + MM – carbon source − − − − MM + peptone − − − − MM + peptone + glucose + + + + + + + + + + + + MM + yeast extract − − − − MM + yeast extract + glucose + + + + + + + + + + + + MM + peptone + yeast extract + glucose + + + + + + + + + + + + MM + casamino acids − − − − MM + casamino acids + glucose + + + + + + + + + + + + MM + casamino acids + peptone + glucose + + + + + + + + + + + + MM + casamino acids + yeast extract + glucose + + + + + + + + + + + + MM + casamino acids + fructose + + + + + + + + + + + + MM + casamino acids + sodium acetate + + + − + + + + + + MM + casamino acids + olive oil + + + + + + + MM + vitamin solution + glucose + + + − + + + + + + MM + vitamin solution + yeast extract + glucose + + + + + + + + + + + + a Complete medium is a rich growth medium containing yeast extract, peptone and casamino acids (see Materials and methods for recipe). CM normally contains glucose as the principal carbon source. The medium was supplemented (+) or depleted (−) as shown. b +++ = extensive hyphal growth and sporulation; ++ = reduced growth; + = very poor growth and sporulation; − = no growth/sparse mycelium, no sporulation. All growth tests were carried out on agar-solidified medium and monitored after 12 days incubation at 24°C. c Minimal medium is described in Materials and methods. Supplementations were made of single or multiple carbon sources as indicated (+). Isolation of NTH1 encoding a neutral trehalase from M.grisea To investigate how stored trehalose in M.grisea is mobilized, we identified a gene encoding the intracellular regulated form of trehalase. A previous study to identify M.grisea genes involved in virulence using insertional mutagenesis had revealed a gene called PTH9, which encodes a protein showing similarity to neutral trehalase (Sweigard et al., 1998). We obtained the pth9 insertion mutant E508-R-6 and a PTH9 genomic clone (kindly provided by Dr J.A.Sweigard, DuPont Company, Wilmington, DE). DNA sequencing defined a 2211 bp ORF encoding a 736 amino acid protein of 84.6 kDa, showing 81% identity to the treB neutral trehalase enzyme from N.crassa and 68% identity to treB from A.nidulans (DDBJ/EMBL/GenBank accession No. AY148092; see Supplementary data). We named the gene NTH1 for neutral trehalase. The NTH1 protein sequence contains a consensus phosphorylation site for cAMP-dependent protein kinase A (PKA) and a putative Ca2+-binding site, indicating that the gene encodes a regulated form of trehalase. To investigate NTH1 expression, RNA gel blots showed that the 2.7 kb NTH1 transcript was 4-fold more abundant during conidiogenesis, than in vegetative hyphae. NTH1 was also expressed abundantly in mycelium after exposure to 0.5 M NaCl for 24 h. The same response was observed after hyperosmotic stress using sorbitol (not shown) and was independent of the presence of the osmoregulatory mitogen-activated protein (MAP) kinase gene OSM1 (Dixon et al., 1999), as shown in Figure 3A. Spatial expression of NTH1 was determined by fusing a 2.5 kb NTH1 promoter fragment upstream of the green fluorescent protein (GFP)-encoding gene sGFP (Chiu et al., 1996) and introducing the plasmid into M.grisea. Transformants containing a single copy of NTH1(p):sGFP were examined by epifluorescence microscopy (Figure 3B). NTH1(p):sGFP expression occurred during initial stages of conidiation when conidiophores develop from a foot cell and differentiate to produce a sympodial array of conidia (Figure 3B–D). Expression was also observed in invasive hyphae within plant tissue, indicating that expression of NTH1 occurs after cuticle penetration (Figure 3E–F). Sequence analysis of the NTH1 promoter revealed putative binding sites for homologues of the stuA and abaA transcriptional activators known to regulate conidiogenesis in A.nidulans (Timberlake, 1993) and three consensus stress response elements (STREs) found in genes expressed during the multistress response in yeast (Schuller et al., 1994). We conclude that NTH1 is expressed during M.grisea sporulation, plant infection and in response to environmental stress. Figure 3.Expression of M.grisea NTH1 during conidiogenesis, plant infection and in response to hyperosmotic stress. (A) RNA was extracted from a non-conidiating culture of M.grisea strain Guy11 (lane 1), a conidiating culture of Guy11 (lane 2) and a conidiating culture of nth1 mutant E508.R.6 (lane 3). RNA was also extracted from hyphal cultures of M.grisea grown in iso-osmotic media, CM (lanes 4 and 6), or subjected to acute hyperosmotic stress in CM + 0.5 M NaCl for 24 h (lanes 5 and 7). RNA was from strain Guy11 (lanes 4 and 5) or Δosm1 mutant JH73 (lanes 6 and 7). RNA gel blots were probed with NTH1. The 28S rRNA is shown as a loading standard. (B) Spatial expression of NTH1. Early conidiogenesis initiated from a foot cell in NTH1(p)::sGFP::Hph transformant T-4-11. (C) Conidial initials forming at the end of a conidiophore. (D) Mature conidiophore with three sympodially arrayed conidia. (E) Germ tube and appressorium on barley epidermis. (F) An invasive hypha entering an epidermal cell from the base of the same appressorium. Bar in all panels = 10 μm. Download figure Download PowerPoint NTH1 encodes a virulence factor for rice blast disease The pth9 insertional mutant E508-R-6 contained a 4.0 kb plasmid insertion in the NTH1 ORF, but theoretically was capable of producing a truncated NTH1 trehalase (Figure 4A and B), although RNA gel blots suggested that the mutant did not produce an NTH1 transcript (Figure 3A). To ensure generation of a null mutant, a gene replacement was carried out by deleting 2.4 kb of NTH1 and replacing this with a hygromycin resistance cassette. DNA gel blots identified three transformants where the gene replacement had occurred from 38 selected (see Supplementary data available at The EMBO Journal Online). The three Δnth1 mutants T-2-8, T-2-30 and T-2-37 were not affected in hyphal growth, response to heat stress or the temporal dynamics of spore germination (not shown). The Δnth1 mutants did show a significant decrease in sporulation, producing ∼50% of the number of conidia in the wild-type Guy11 (t = 8.77, P < 0.001, df = 30). To test the effect of Δnth1 deletion on rice blast disease, conidial suspensions of three mutants and Guy11 (at identical concentrations) were sprayed onto seedlings of rice cultivar CO-39. The Δnth1 mutants all produced significantly fewer disease lesions than Guy11 (Figure 4). The Δnth1 mutants showed lesion densities that were 24 ± 16% of those produced by Guy11 (t-tests of lesion densities were significant at P < 0.001, n = 100 leaves). The pathogenicity of Δnth1 mutants on barley seedlings showed a similar reduction. To determine the likely cause of the pathogenicity defect, we assayed cuticle penetration and appressorium turgor generation (see Supplementary data). No significant differences were observed compared with the wild-type strain, indicating that the ability to infect plants was not compromised by the Δnth1 mutation. Instead, we observed that proliferation of invasive hyphae was not as rapid in Δnth1 mutants as in Guy11 (not shown). Re-introduction of NTH1 into a Δnth1 mutant restored pathogenicity and conidiogenesis to wild-type levels (not shown). We conclude that NTH1 is required by M.grisea to generate severe rice blast symptoms. Figure 4.Virulence phenotype of M.grisea Δnth1 mutants. Seedlings of rice cultivar CO-39 were inoculated with M.grisea conidial suspensions of identical concentration (2 × 104 conidia/ml) of strain Guy11 (A), and Δnth1 mutants T-2-8, T-2-30 and T-2-37 (B–D). Seedlings were incubated for 4 days for development of disease. Download figure Download PowerPoint TRE1 encodes the major trehalase activity in germlings and hyphae of M.grisea The temporal expression pattern of NTH1 and reduction in conidiation in Δnth1 mutants pointed to a potential role for NTH1 in mobilization of trehalose during spore germination. To investigate this, we measured the amount of trehalose in conidia and germlings of M.grisea at three different times during germination (Figure 5A). In Guy11, trehalose was mobilized rapidly during germination, and almost completely degraded after 2 h. A similar pattern of trehalose degradation was observed in Δnth1 mutant T-2-8, although the initial amount of trehalose in conidia was less than in Guy11. The pattern of trehalose degradation in Δnth1 mutants suggested to us that M.grisea must possess another intracellular trehalase activity, and we therefore assayed neutral trehalase activity at the same times during conidial germination. The Δnth1 mutant showed no significant difference in neutral trehalase activity during conidial germination when compared with Guy11 (Figure 5B), confirming the presence of a second trehalase in M.grisea. Figure 5.Trehalose mobilization and neutral trehalase activity during conidial germination of M.grisea. (A) Line graph of trehalose levels in conidia of Guy11 (diamonds), Δnth1 mutant T-2-8 (squares) and tre1::Hph mutant T-8-45 (triangles) at three intervals during germination. Data points represent the mean of three replications of the experiment. (B) Bar chart of neutral trehalase activity in conidia in the same strains at identical intervals. Each bar represents the mean of three replicate assays. (C) Neutral trehalase activity in vegetative hyphae of M.grisea assayed using an overlay test (see Materials and methods). Cultures of Guy11, Δnth1 mutant T-2-8, tre1::Hph mutant T-8-45 and Δtps1 mutant T-5-13 were grown for 6 days prior to assay. All experiments were repeated at least three times with similar results. Download figure Download PowerPoint To identify the second trehalase, we used a bioinformatic approach and scanned all available DNA sequence information from M.grisea for homology to eukaryotic trehalase-encoding enzymes. We found a small fragment of a putative trehalase-encoding gene in a sequence from a bacterial artificial chromosome, used in the M.grisea genome mapping effort (Zhu et al., 1999). We amplified a corresponding 504 bp gene fragment and used this to isolate a cDNA and genomic clone spanning the locus. DNA sequencing revealed a 1914 bp ORF interrupted by four putative introns, capable of encoding a 698 amino acid protein (Figure 6A) with a putative signal peptide of 22 amino acids at the N-terminus. We named the gene TRE1 (trehalase-encoding). The putative TRE1 product showed 33% identity to the human and mouse TreA trehalases, but was distinct from both neutral and acidic trehalases previously identified from fungi (Figure 6B). Scanning the publicly available genome sequence of N.crassa revealed a gene showing 66% identity to TRE1, but no homologous sequence was observed in the Aspergillus fumigatus draft genome sequence. Thus, only closely related filamentous fungi (N.crassa and M.grisea are both pyrenomycetes) appear to possess this novel form of trehalase. To determine the cellular location of the enzyme, we produced a TRE1:GFP gene fusion by introducing the sGFP allele at the C-terminus of the TRE1 ORF. The gene fusion was transformed into Guy11, and transformants containing a single copy of the TRE1:GFP fusion were selected (Figure 7). TRE1:GFP accumulates in the septa of germinating conidia (Figure 7A–F), the germ tube apex and the walls of developing appressoria (Figure 7H and K). The temporal pattern of TRE1:GFP accumulation was consistent with RNA gel blots which showed TRE1 expression during the early stages of conidial germination (not shown). A targeted disruption of TRE1 utilized an adaptation of an in vitro transposon tagging procedure previously described (Hamer et al., 2001). We modified the Tn7 transposable element of pGPS3 (New England Biolabs), by introducing the hygromycin B resistance gene cassette. The modified transposable element was mobilized in vitro, allowing us to introduce transposon insertions throughout a cloned 8 kb genomic DNA fragment spanning the TRE1 locus. A gene disruption construct containing the 3 kb GPS-HYG transposon insertion at the 5′ end of the TRE1 coding region was introduced into M.grisea strain Guy11, and 56 hygromycin-resistant transformants were selected. Twenty-seven of these (48%) showed the presence of the gene disruption, with the 8 kb TRE1 locus replaced by the 11 kb tre1::Hph insertion allele (see Supplementary data). Figure 6.Predicted amino acid sequence of the M.grisea TRE1 gene product. (A) Sequences were aligned using Clustal W (Thompson et al., 1994). The TRE1 gene product is aligned with sequences of N.crassa hypothetical protein NCU00943.1 (http://www-genome.wi.mit.edu/annotation/fungi/neurospora/), rabbit TreA (P19823), Escherichia coli treA (P13482) and S.cerevisiae NTH1 (P321356). Identical amino acids are highlighted on a black background, and similar amino acids on a light grey background. Gaps are indicated by dashes. M.grisea TRE1 has DDBJ/EMBL/GenBank accession No. AF543465. (B) Dendrogram indicating the relationship of TRE1 to previously identified trehalase gene products. Genetic distance is proportional to the length of the horizontal line. Download figure Download PowerPoint Figure 7.Localization of TRE1 trehalase during infection-related development of M.grisea. Conidia of Guy11 TRE1:sGFP transformant T-9-14 were allowed to form appressoria on plastic surfaces. Bright-field images are shown in the left-hand panels, epifluorescence images in the centre and merged images in the right-hand panels. (A–C) A three-celled conidium undergoing germination and germ tube extension 2 h after inoculation; TRE1:GFP accumulates in septa and germ tube apex. (D–F) A germ tube extension with septal localization of TRE1:GFP, 2 h after inoculation (G–I) A germ tube undergoing hooking and appressorium formation after 6 h. The appressorium is the round structure at the end of the fungal germ tube. (J–L) Appressorium maturation with wall localization of TRE1:GFP after 7 h. Bar = 10 μm.