Title: IL-1 and TNF Induced Up-Regulation of IL-8 Receptors on Human Epidermal Cells
Abstract: Interleukin-8 (IL-8) is a novel cytokine with potent proinflammatory and mitogenic properties. Recently, we have found that IL-8 is chemotactic for epidermal cells, and human keratinocytes possess specific receptors for IL-8 (IL-8-R). Since IL-1α and tumor necrosis factor-α (TNF-α) are capable of modulating various cell surface receptors of keratinocytes, we have studied the effects of these cytokines on the IL-8-R expression of human epidermal cells. A transformed epidermal cell line (SCL-II cells), freshly separated and cultured normal human keratinocytes were used for the studies. The IL-8-R has been detected by flow cytometry using phycoerythrin-labeled IL-8 (IL-8-PE). Pretreatment of cells with IL-lα and/or TNF-α induced a dose-dependent increase in specific IL-8-PE binding to the cells. The observed increase in specific IL-8-PE binding was also time-dependent, reaching its maximum after a 24-hour pretreatment time. The up-regulation of IL-8-R on human epidermal cells by IL-lα and TNF-α indicates a very complex interaction between these inflammatory cytokines.
Publication Year: 1993
Publication Date: 1993-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 3
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