Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1992092667', 'doi': 'https://doi.org/10.1016/0003-2697(83)90017-9', 'title': 'A fast silver staining method for protein detection after isoelectric focusing in agarose gels', 'display_name': 'A fast silver staining method for protein detection after isoelectric focusing in agarose gels', 'publication_year': 1983, 'publication_date': '1983-07-01', 'ids': {'openalex': 'https://openalex.org/W1992092667', 'doi': 'https://doi.org/10.1016/0003-2697(83)90017-9', 'mag': '1992092667', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/6194715'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/0003-2697(83)90017-9', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S7858788', 'display_name': 'Analytical Biochemistry', 'issn_l': '0003-2697', 'issn': ['0003-2697', '1096-0309'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5085676874', 'display_name': 'Françoise Lasne', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210123600', 'display_name': 'Hôpital Edouard Herriot', 'ror': 'https://ror.org/02qt1p572', 'country_code': 'FR', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210100596', 'https://openalex.org/I4210123600']}, {'id': 'https://openalex.org/I4210130154', 'display_name': 'Laboratoire de Chimie', 'ror': 'https://ror.org/02gaw1s20', 'country_code': 'FR', 'type': 'facility', 'lineage': ['https://openalex.org/I100532134', 'https://openalex.org/I113428412', 'https://openalex.org/I1294671590', 'https://openalex.org/I203339264', 'https://openalex.org/I4210128300', 'https://openalex.org/I4210130154']}, {'id': 'https://openalex.org/I100532134', 'display_name': 'Université Claude Bernard Lyon 1', 'ror': 'https://ror.org/029brtt94', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I100532134', 'https://openalex.org/I203339264']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Francoise Lasne', 'raw_affiliation_strings': ['Laboratoire de Chimie Biologique, U.E.R. Grange-Blanche, Université Claude Bernard Lyon I, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France'], 'affiliations': [{'raw_affiliation_string': 'Laboratoire de Chimie Biologique, U.E.R. Grange-Blanche, Université Claude Bernard Lyon I, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France', 'institution_ids': ['https://openalex.org/I4210123600', 'https://openalex.org/I4210130154', 'https://openalex.org/I100532134']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5051748382', 'display_name': 'Odette Benzerara', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210123600', 'display_name': 'Hôpital Edouard Herriot', 'ror': 'https://ror.org/02qt1p572', 'country_code': 'FR', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210100596', 'https://openalex.org/I4210123600']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Odette Benzerara', 'raw_affiliation_strings': ["Laboratoire Central des Isotopes No 21411 M, Hôpital Edouard Herriot, Place d'Arsonval, 69374 Lyon Cedex 03, France"], 'affiliations': [{'raw_affiliation_string': "Laboratoire Central des Isotopes No 21411 M, Hôpital Edouard Herriot, Place d'Arsonval, 69374 Lyon Cedex 03, France", 'institution_ids': ['https://openalex.org/I4210123600']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5038091768', 'display_name': 'Y Lasne', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210123600', 'display_name': 'Hôpital Edouard Herriot', 'ror': 'https://ror.org/02qt1p572', 'country_code': 'FR', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210100596', 'https://openalex.org/I4210123600']}, {'id': 'https://openalex.org/I4210130154', 'display_name': 'Laboratoire de Chimie', 'ror': 'https://ror.org/02gaw1s20', 'country_code': 'FR', 'type': 'facility', 'lineage': ['https://openalex.org/I100532134', 'https://openalex.org/I113428412', 'https://openalex.org/I1294671590', 'https://openalex.org/I203339264', 'https://openalex.org/I4210128300', 'https://openalex.org/I4210130154']}, {'id': 'https://openalex.org/I100532134', 'display_name': 'Université Claude Bernard Lyon 1', 'ror': 'https://ror.org/029brtt94', 'country_code': 'FR', 'type': 'education', 'lineage': ['https://openalex.org/I100532134', 'https://openalex.org/I203339264']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Yves Lasne', 'raw_affiliation_strings': ['Laboratoire de Chimie Biologique, U.E.R. Grange-Blanche, Université Claude Bernard Lyon I, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France'], 'affiliations': [{'raw_affiliation_string': 'Laboratoire de Chimie Biologique, U.E.R. Grange-Blanche, Université Claude Bernard Lyon I, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France', 'institution_ids': ['https://openalex.org/I4210123600', 'https://openalex.org/I4210130154', 'https://openalex.org/I100532134']}]}], 'countries_distinct_count': 1, 'institutions_distinct_count': 3, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 3320, 'currency': 'USD', 'value_usd': 3320, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 1.761, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 12, 'citation_normalized_percentile': {'value': 0.618835, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 81, 'max': 82}, 'biblio': {'volume': '132', 'issue': '2', 'first_page': '338', 'last_page': '341'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10519', 'display_name': 'Mass Spectrometry Techniques with Proteins', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10519', 'display_name': 'Mass Spectrometry Techniques with Proteins', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10602', 'display_name': 'Glycosylation in Health and Disease', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11970', 'display_name': 'Real-Time Polymerase Chain Reaction', 'score': 0.9985, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/agarose', 'display_name': 'Agarose', 'score': 0.9144505}, {'id': 'https://openalex.org/keywords/silver-stain', 'display_name': 'Silver stain', 'score': 0.74127495}, {'id': 'https://openalex.org/keywords/coomassie-brilliant-blue', 'display_name': 'Coomassie Brilliant Blue', 'score': 0.6847276}, {'id': 'https://openalex.org/keywords/protein-identification', 'display_name': 'Protein Identification', 'score': 0.508714}, {'id': 'https://openalex.org/keywords/protein-detection', 'display_name': 'Protein detection', 'score': 0.43203273}, {'id': 'https://openalex.org/keywords/immobilized-ph-gradient', 'display_name': 'Immobilized pH gradient', 'score': 0.41584468}], 'concepts': [{'id': 'https://openalex.org/C2778476535', 'wikidata': 'https://www.wikidata.org/wiki/Q390697', 'display_name': 'Agarose', 'level': 2, 'score': 0.9144505}, {'id': 'https://openalex.org/C50476208', 'wikidata': 'https://www.wikidata.org/wiki/Q898861', 'display_name': 'Isoelectric focusing', 'level': 3, 'score': 0.90587556}, {'id': 'https://openalex.org/C74864618', 'wikidata': 'https://www.wikidata.org/wiki/Q2332446', 'display_name': 'Staining', 'level': 2, 'score': 0.89502454}, {'id': 'https://openalex.org/C25330733', 'wikidata': 'https://www.wikidata.org/wiki/Q907841', 'display_name': 'Silver stain', 'level': 2, 'score': 0.74127495}, {'id': 'https://openalex.org/C2780761556', 'wikidata': 'https://www.wikidata.org/wiki/Q418516', 'display_name': 'Coomassie Brilliant Blue', 'level': 3, 'score': 0.6847276}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.6330009}, {'id': 'https://openalex.org/C96294017', 'wikidata': 'https://www.wikidata.org/wiki/Q308708', 'display_name': 'Isoelectric point', 'level': 3, 'score': 0.5880011}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.57760864}, {'id': 'https://openalex.org/C3019566124', 'wikidata': 'https://www.wikidata.org/wiki/Q85794778', 'display_name': 'Protein detection', 'level': 2, 'score': 0.43203273}, {'id': 'https://openalex.org/C154337534', 'wikidata': 'https://www.wikidata.org/wiki/Q6005260', 'display_name': 'Immobilized pH gradient', 'level': 4, 'score': 0.41584468}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.3817853}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.30669796}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.15675491}, {'id': 'https://openalex.org/C192562407', 'wikidata': 'https://www.wikidata.org/wiki/Q228736', 'display_name': 'Materials science', 'level': 0, 'score': 0.13765877}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.05577612}, {'id': 'https://openalex.org/C171250308', 'wikidata': 'https://www.wikidata.org/wiki/Q11468', 'display_name': 'Nanotechnology', 'level': 1, 'score': 0.042437196}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D012834', 'descriptor_name': 'Silver', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D001613', 'descriptor_name': 'beta 2-Microglobulin', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D007525', 'descriptor_name': 'Isoelectric Focusing', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012394', 'descriptor_name': 'Rosaniline Dyes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013194', 'descriptor_name': 'Staining and Labeling', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001613', 'descriptor_name': 'beta 2-Microglobulin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1016/0003-2697(83)90017-9', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S7858788', 'display_name': 'Analytical Biochemistry', 'issn_l': '0003-2697', 'issn': ['0003-2697', '1096-0309'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/6194715', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 9, 'referenced_works': ['https://openalex.org/W1606423922', 'https://openalex.org/W2018895033', 'https://openalex.org/W2022753715', 'https://openalex.org/W2044936649', 'https://openalex.org/W2071621524', 'https://openalex.org/W2077371019', 'https://openalex.org/W2078988081', 'https://openalex.org/W2080405622', 'https://openalex.org/W2224139360'], 'related_works': ['https://openalex.org/W2410442203', 'https://openalex.org/W2393800496', 'https://openalex.org/W2320241687', 'https://openalex.org/W2084174258', 'https://openalex.org/W2070744277', 'https://openalex.org/W2047585996', 'https://openalex.org/W2044181871', 'https://openalex.org/W2024699216', 'https://openalex.org/W1992092667', 'https://openalex.org/W1967554868'], 'abstract_inverted_index': {'A': [0], 'sensitive': [1], 'staining': [2, 27], 'method': [3], 'was': [4], 'developed': [5], 'for': [6], 'detecting': [7], 'proteins': [8], 'in': [9], 'agarose': [10], 'gels': [11], 'after': [12], 'isoelectric': [13], 'focusing.': [14], 'Its': [15], 'sensitivity': [16], 'is': [17, 33], 'about': [18], '20': [19], 'times': [20], 'that': [21], 'of': [22], 'the': [23, 30], 'Coomassie': [24], 'blue': [25], 'R-250': [26], 'technique,': [28], 'and': [29], 'time': [31], 'required': [32], 'only': [34], '10': [35], 'min.': [36]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1992092667', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}], 'updated_date': '2024-08-14T07:53:27.652990', 'created_date': '2016-06-24'}