Title: <i>N</i>‐Methyl‐<scp>d</scp>‐Aspartate‐ or Glutamate‐Mediated Toxicity in Cultured Rat Cortical Neurons Is Antagonized by FPL 15896AR
Abstract: Abstract: The neuroprotective action of ( S )‐α‐phenyl‐2‐pyridineethanamine dihydrochloride (FPL 15896AR), a novel noncompetitive N ‐methyl‐ d ‐aspartate (NMDA) receptor antagonist, was examined in primary rat cortical neuronal cultures. Exposure of cortical cultures to NMDA (50 µ M ) or glutamate (50 µ M ) for 15 min resulted in the death of 85–95% of the neurons during the next 24 h. This neurotoxicity was completely eliminated by adding FPL 15896AR (50 µ M ) to the cultures during the time of NMDA or glutamate exposure. Neuroprotective concentrations of FPL 15896AR also inhibited other acute effects of NMDA. FPL 15896AR (50 µ M ) prevented the loss of membrane‐associated protein kinase C activity that developed by 4 h after transient exposure to 50 µ M NMDA or 50 µ M glutamate. FPL 15896AR also reduced by ∼35% the magnitude of NMDA‐triggered increases in intracellular free Ca 2+ concentration in the cortical cultures. These data indicate that NMDA‐mediated toxicity in cultured cortical neurons can be blocked by the NMDA antagonist FPL 15896AR.
Publication Year: 1995
Publication Date: 1995-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 34
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