Title: High-performance liquid chromatography of two derivatives of vitamin B6, the carbamoyl derivatives of pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate
Abstract: We present the results of a study of the high-performance liquid chromatography of two vitamin B6 derivatives: the carbamoyl derivatives of pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate. These compounds, obtained by condensation of either pyridoxal 5′-phosphate or pyridoxamine 5′-phosphate with either carbamoyl phosphate or potassium cyanate, were separated on an octadecyl silica column with a mobile phase consisting of 0.01 M potassium phosphate (pH 5.0)—methanol (98:2); detection was at 254 nm. The method was sensitive, fast and precise.
Publication Year: 1991
Publication Date: 1991-06-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 3
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