Title: Catalytic behavior and detoxifying ability of an atypical homotrimeric laccase from the thermophilic strain Scytalidium thermophilum on selected azo and triarylmethane dyes
Abstract: Laccase catalyzed decolorization and detoxification was investigated for the azo dye Congo Red and three triarylmethane dyes: Malachite Green, Bromo-Cresol Green and Phenol Red, commonly found in textile industry effluents. In the present work, we studied various physico-chemical parameters such as pH, temperature, ionic strength and initial dye concentration to achieve maximum dye decolorization by Scytalidium thermophilum laccase. The enzyme efficiently decolorized dyes from 46% to 99% at 50 mg/l of initial dye concentration in the presence of 0.1 U/ml of laccase. Under optimized conditions, Km values of the enzyme were 0.25; 3.31; 5.61 and 172.1 mM toward Phenol Red, Congo Red, Malachite Green and Bromo-Cresol Green, respectively. Among these four dyes, Bromo-Cresol Green at a concentration of 10,000 mg/l showed the best decolorization reaching 83% within 6 h. Cytotoxicity and microtoxicity evaluation demonstrated a decreased toxicity of the final degraded products.
Publication Year: 2012
Publication Date: 2012-04-04
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 40
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