Abstract: The use of DNA typing for the examination of forensic casework and the establishment of paternity is discussed. A description is given of DNA-based identification testing procedures, most of which involve the use of restriction endonucleases, DNA probes, and Southern blot hybridization. Restriction endonucleases are enzymes that cleave a person's DNA, at specific recognition sites, into a reproducible pattern of fragments. DNA probes are relatively small pieces of DNA (also known as oligonucleotides), which are selected because they recognize specific genetic sequences of interest on a segment of target DNA. The probe and detected sequence (known as a complementary sequence) fit together specifically like the two halves of a zipper. Southern blot hybridization is essentially the process by which specific target DNA fragments of interest are isolated, detected, and visualized. The differences between testing for a crime scene investigation and testing the paternity establishment are considered. The impact of DNA testing is discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Publication Year: 1989
Publication Date: 1989-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 1
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