Publication Information

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3813, 3976, 4003, 4591], 'this': [40, 176, 485, 607, 670, 1020, 1086, 1388, 1690, 2721, 2805, 3060, 3439, 3501, 3584, 3881, 4243], 'method': [41, 177], 'vivo': [43, 64, 131, 179, 200, 267, 858, 894, 2674, 3893, 4368], 'on': [44, 180, 467, 1132, 1535, 1542, 1718, 1867, 1921, 1950, 2125, 3164, 3506, 3837, 3912, 3951], 'milligram': [45, 181], 'scales.': [46, 182], 'There': [47, 183, 3985], 'no': [49, 185, 459, 2056, 2385, 3548, 3909, 4234, 4267, 4459, 4609], 'by-product': [50, 186], 'linear': [52, 78, 188, 214, 299, 1675, 1729, 2547, 2558, 2610, 2656, 3871, 3920, 4276, 4309, 4382, 4461, 4606, 4645], 'or': [53, 62, 189, 198, 542, 1798, 2234, 2243, 2440, 2945, 2948, 3430, 3740, 3830], 'polymerized': [54, 190], 'species': [55, 191], 'isolated,': [56, 192], 'unlike': [57, 193], 'other': [59, 195, 700, 768, 4298], 'vitro': [61, 197, 425, 2823, 4300], 'cyclization': [65, 201, 421, 761, 835, 859, 927, 1094, 1120, 2897, 4301, 4369, 4534, 4624], 'methods': [66, 202], 'utilizing': [67, 203], 'inteins.': [68, 204], 'Cyclized': [69, 205], 'GFP': [70, 105, 206, 241, 1124, 1863, 1872, 2933, 2978, 3057, 3186, 3535, 4211], 'unfolded': [71, 207], 'at': [72, 208, 631, 657, 684, 1416, 1443, 1449, 1488, 1494, 1551, 1558, 1712, 1762, 1792, 2173, 2204, 2277, 2289, 2348, 2388, 2406, 2449, 2623, 2628, 2736, 3686, 3728, 3762, 3865, 3915, 4192, 4197, 4270], 'half': [73, 209], 'rate': [75, 211, 1446], 'form': [79, 215, 1322, 1676, 1686, 1737, 3601, 3886, 4063, 4250, 4383, 4462, 4503], 'upon': [80, 216, 2626, 4647], 'chemical': [81, 217, 476], 'denaturation': [82, 218, 2568, 2597], 'and': [83, 117, 128, 219, 253, 264, 325, 340, 401, 418, 531, 568, 756, 774, 837, 873, 891, 930, 947, 1058, 1137, 1181, 1188, 1227, 1233, 1238, 1263, 1269, 1302, 1362, 1429, 1476, 1515, 1532, 1575, 1638, 1781, 1790, 1802, 1843, 1849, 1989, 2009, 2037, 2044, 2079, 2154, 2165, 2220, 2239, 2253, 2295, 2318, 2366, 2372, 2494, 2503, 2548, 2559, 2603, 2611, 2679, 2698, 2728, 2779, 2782, 2818, 2959, 2991, 3145, 3159, 3174, 3190, 3272, 3319, 3334, 3354, 3372, 3394, 3410, 3422, 3475, 3491, 3595, 3653, 3666, 3679, 3698, 3726, 3738, 3749, 3770, 3788, 3800, 3822, 3864, 3901, 3944, 3962, 3994, 4029, 4034, 4043, 4047, 4052, 4068, 4168, 4187, 4310, 4525, 4543, 4560, 4577], 'required': [84, 220], '>2': [85, 221], 'days': [86, 222], '7m': [88, 224, 2570], 'guanidine': [89, 225, 287], 'hydrochloride': [90, 226, 288], 'until': [91, 227], 'residual': [93, 229, 2386], 'fast': [94, 230, 2502, 2512, 2635], 'folding': [95, 231, 3483], 'phase': [96, 232, 1644, 1651, 2513, 2636], '(consistent': [97, 233], 'persistent': [100, 236], 'cis-proline)': [101, 237], 'had': [102, 238, 2979, 4182], 'disappeared.': [103, 239], 'Cyclic': [104, 240], 'might': [106, 242, 1125, 2934, 3433, 3934, 4498, 4521, 4530], 'become': [107, 243, 1126], 'tool': [110, 246, 1129, 2938], 'for': [111, 247, 346, 587, 866, 883, 895, 901, 926, 1130, 1395, 1478, 1491, 1529, 1555, 1709, 1778, 1795, 1829, 1938, 2013, 2217, 2403, 2446, 2470, 2500, 2896, 2939, 3530, 3731, 3765, 4603, 4613, 4655], 'studying': [112, 248, 2940], 'role': [114, 250], 'backbone': [118, 254, 320, 523, 2950], 'topology': [119, 255, 351, 524, 2951], 'various': [121, 257, 2319, 2447], 'biological': [122, 258], 'processes': [123, 259, 2954], 'such': [124, 260, 771, 2955], 'as': [125, 132, 261, 268, 525, 527, 654, 691, 693, 772, 898, 900, 1118, 1605, 1901, 1924, 1926, 1945, 1994, 2007, 2022, 2042, 2590, 2956, 3385, 3689, 3810, 3812, 3869, 3873, 4021], 'degradation': [127, 263, 403, 2958, 4545], 'translocation': [129, 265, 2960], 'well': [133, 269, 526, 692, 899, 1925, 3811], 'asin': [134, 270], 'vitro.': [135, 271, 533], 'insert': [273], 'green': [274, 278, 1114, 1503, 2905, 2975, 3701, 4109, 4185], 'improved': [277, 358, 423, 1253, 2904], 'high': [282], 'solubility': [283], 'nitrilotriacetic': [284], 'acid': [285, 4596], 'dithiothreitol': [286], 'polymerase': [289, 1294, 1305], 'chain': [290, 1295, 1306], 'reaction': [291, 630, 748, 762, 1296, 1307, 1749, 2575], 'All': [292, 1986, 2100, 2169, 2193, 2615], 'natural': [293, 2680], 'proteins': [294, 510, 530, 872, 892, 929, 1100, 1581, 1820, 2396, 3973], 'known': [295], 'so': [296], 'far': [297], 'are': [298, 2368, 2496, 3148, 3162, 3187, 3624, 4037], 'chains': [300], 'amino': [302, 316, 2026, 3002, 3403, 3512, 3611, 3631, 3646, 3657, 4595], 'acids': [303, 2027, 3003, 3513, 3612], 'that': [304, 393, 419, 675, 912, 1054, 2804, 3042, 3059, 3445, 3621, 3842, 3880, 3906, 4099, 4196, 4242, 4264, 4513, 4622], 'fold': [305, 4481], 'into': [306, 1061, 1078, 2743, 3484, 4486], 'unique': [308, 4559], 'three-dimensional': [309], 'structure': [310, 321, 1081, 1860, 1870, 1905, 1919, 1965, 2004, 2015, 2019, 2094, 3055, 3066, 3487, 3532, 4506], 'dictated': [311], 'sequence': [314, 3256, 3294, 3322, 3343, 4571, 4576, 4583, 4601, 4610, 4633], 'acids.': [317], 'has': [322, 388, 514, 536, 672, 764, 2801, 3062, 4479], 'been': [323, 390, 537, 2802, 2980], 'found': [324, 2000, 3526], 'synthetically': [326], 'introduced': [327, 2041, 3301, 3308], 'small': [329], 'peptides,': [330], 'but': [331, 758, 1032, 2592, 2753, 3161, 3540, 3922, 4458, 4608], 'is': [332, 352, 619, 704, 2675, 2687, 2751, 3264, 3344, 3383, 3396, 3466, 3619, 3883, 3890, 3904, 4096, 4203, 4246, 4493, 4652], 'an': [333, 609, 612, 650, 655, 1844, 2287, 2649, 2685, 3325, 3364, 3425, 3636, 3662, 3781, 3789, 4295], 'almost': [334], 'unexplored': [335], 'topic': [336], 'chemistry': [339], 'structural': [342], 'research,': [343], 'particular': [345], 'larger': [347], 'proteins.': [348, 906], 'The': [349, 646, 1065, 1143, 1190, 1235, 1249, 1304, 1385, 1412, 1438, 1482, 1497, 1519, 1537, 1561, 1579, 1596, 1642, 1656, 1674, 1723, 1809, 1819, 1859, 1903, 1932, 1997, 2017, 2031, 2139, 2179, 2215, 2257, 2292, 2351, 2463, 2473, 2508, 2528, 2574, 2644, 2677, 3053, 3142, 3171, 3254, 3316, 3340, 3389, 3399, 3519, 3671, 3694, 3772, 3824, 3855, 4001, 4014, 4032, 4180, 4533, 4551, 4627, 4638], 'also': [353, 422, 879, 1033, 1258, 2714, 4653], 'expected': [354, 3620, 4654], 'lead': [356, 3049], 'stability': [359, 427], 'due': [360, 1400, 3495], 'reduced': [363], 'conformational': [364], 'entropy': [365], 'denatured': [368, 2399, 2441], 'state,': [369], 'according': [370], 'polymer': [372], 'theory': [373], '(1Flory': [374], 'P.J.': [375], 'J.': [376, 489, 573, 734, 798, 813, 816, 997, 1002, 1167, 1213, 1976, 2114, 2834, 2861, 2882, 3028, 3565, 4331, 4346, 4349, 4425, 4430], 'Am.': [377, 799, 2862, 4332], 'Chem.': [378, 800, 818, 1004, 2863, 3567, 4333, 4351, 4432], 'Soc.': [379, 801, 2864, 4334], '1956;': [380], '78:': [381], '5222-5235Crossref': [382], 'Scopus': [383, 414, 452, 496, 564, 582, 602, 726, 743, 790, 805, 828, 852, 977, 1014, 1174, 1220, 1285, 1626, 1897, 1982, 2121, 2423, 2436, 2841, 2868, 2889, 2927, 3021, 3037, 3100, 3121, 3138, 3212, 3233, 3250, 3577, 4147, 4227, 4323, 4338, 4361, 4405, 4442], '(367)': [384], 'Google': [385, 416, 454, 498, 566, 584, 604, 728, 745, 792, 807, 830, 854, 979, 1016, 1176, 1222, 1287, 1628, 1899, 1984, 2123, 2425, 2438, 2843, 2870, 2891, 2929, 3023, 3039, 3102, 3123, 3140, 3214, 3235, 3252, 3579, 4149, 4229, 4325, 4340, 4363, 4407, 4444], 'Scholar).': [386, 455, 499, 605, 746, 855, 1017, 1629, 1985, 2892, 3141, 3253, 3580, 4150, 4230, 4364, 4445], 'It': [387, 3903, 4095, 4260], 'indeed': [389, 4247], 'shown': [391, 3514, 4241], 'experimentally': [392], 'β-lactamase': [395], 'stabilized': [397], 'against': [398], 'heat': [399], 'precipitation': [400], 'exopeptidase': [402], '(2Iwai': [404, 780, 842, 4313], 'H.': [405, 781, 843, 2413, 2860, 4314], 'Plückthun': [406, 782, 844, 1346, 4315], 'A.': [407, 447, 559, 721, 783, 845, 972, 1272, 1341, 1347, 1881, 1889, 2914, 3016, 3084, 3092, 3196, 3204, 3518, 4316, 4400], 'FEBS': [408, 784, 846, 3031, 4317], 'Lett.': [409, 785, 847, 3032, 4318], '1999;': [410, 448, 786, 802, 819, 848, 973, 1170, 1216, 2837, 3017, 3033, 4319, 4335, 4352, 4401], '459:': [411, 787, 849, 4320], '166-172Crossref': [412, 788, 850, 4321], 'PubMed': [413, 451, 495, 563, 581, 601, 725, 742, 789, 827, 851, 976, 1013, 1173, 1219, 1284, 1625, 1896, 2120, 2422, 2435, 2840, 2888, 2926, 3020, 3036, 3099, 3120, 3137, 3211, 3232, 3249, 3576, 4146, 4322, 4360, 4404, 4441], '(162)': [415, 791, 853, 2424, 4324], 'Scholar)': [417, 1177, 1223, 1358, 1900, 2124, 2439, 2930], 'thermal': [426], 'dihydrofolate': [429], 'reductase': [430], '(3Scott': [431, 956, 4384], 'C.P.': [432, 957, 4385], 'Abel-Santos': [433, 958, 4386], 'E.': [434, 594, 959, 1276, 1392, 1753, 2918, 4387], 'Wall': [435, 960, 4388], 'M.': [436, 961, 1973, 2415, 2877, 3105, 3217, 4389], 'Wahnon': [437, 962, 4390], 'D.C.': [438, 963, 4391], 'Benkovic': [439, 964, 4392], 'S.J.': [440, 965, 3115, 3227, 4393], 'Proc.': [441, 553, 715, 966, 3010, 4394], 'Natl.': [442, 554, 716, 967, 3011, 4395], 'Acad.': [443, 555, 717, 968, 3012, 4396], 'Sci.': [444, 556, 577, 718, 738, 969, 3013, 4397], 'U.': [445, 557, 719, 970, 3014, 4398], 'S.': [446, 558, 720, 971, 3015, 3026, 4399], '96:': [449, 974, 3018, 4402], '13638-13643Crossref': [450, 975, 4403], '(329)': [453, 978, 4406], 'Although': [456], 'there': [457, 2054, 3907, 4154, 4232, 4265], 'significant': [460, 4460], 'stabilization': [461], 'observed': [462, 3444, 4448], 'pioneering': [465], 'work': [466], 'bovine': [470], 'pancreatic': [471], 'trypsin': [472], 'inhibitor': [473], 'prepared': [474, 1680, 2143, 2224, 3714, 3827], 'modification,': [477], 'several': [478], 'effects': [479], 'may': [480, 878], 'have': [481, 910, 1089, 3443, 3504, 4240, 4447, 4557, 4630], 'canceled': [482], 'out': [483], 'example': [486], '(4Goldenberg': [487], 'D.P.': [488], 'Cell.': [490], 'Biochem.': [491], '1985;': [492], '29:': [493], '321-335Crossref': [494], '(47)': [497], 'recent': [501], 'development': [502], 'chemistry,': [505], 'use': [507, 1386], 'self-splicing': [509], '(often': [511], 'called': [512, 538, 1259, 2910], 'inteins),': [513], 'opened': [515], 'general': [517], 'avenue': [518], 'ligate': [529], 'peptidesin': [532], 'This': [534, 625, 747, 877, 1043, 2382, 2750, 3465, 4292], 'procedure': [535, 841], 'expressed': [539, 1442, 3682, 3950], 'ligation': [541, 545], 'intein-mediated': [543], '(Refs.': [546], '5Muir': [547], 'T.W.': [548, 710, 797, 4330], 'Sondhi': [549, 711], 'D.': [550, 712, 985, 989, 4413, 4417], 'Cole': [551, 713], 'P.A.': [552, 714], '1998;': [560, 578, 722, 739, 1622, 2865, 4143], '95:': [561, 723], '6705-6710Crossref': [562, 724], '(972)': [565, 727], 'Scholar': [567, 585], '6Evans': [569, 730], 'Jr.,': [570, 731, 810, 982, 3130, 3242, 4343, 4410], 'T.C.': [571, 732, 811, 983, 4344, 4411], 'Benner': [572, 733, 812, 996, 4345, 4424], 'Xu': [574, 735, 814, 1000, 4347, 4428], 'M.Q.': [575, 736, 815, 1001, 4348, 4429], 'Protein': [576, 737, 1619, 4140], '7:': [579, 740], '2256-2264Crossref': [580, 741], '(334)': [583, 744], 'and,': [586, 2735], 'review,': [588], 'see': [589], 'Ref.': [590], '7Perler': [591], 'F.B.': [592], 'Adam': [593], 'Curr.': [595], 'Opin.': [596], 'Biotechnol.': [597, 1280, 2922, 3133, 3245], '2000;': [598, 1005, 2419, 2885, 4433], '11:': [599], '377-383Crossref': [600], '(65)': [603], 'In': [606, 1018, 1085, 2323, 2391, 2601, 3500], 'approach,': [608], 'asparagine-to-alanine': [613], 'mutation': [614, 626], 'active': [617], 'site': [618, 3288, 4563], 'fused': [620], 'one': [622, 689, 2717], 'fusion': [623, 640, 1324], 'partner.': [624], 'stops': [627], 'enzymatic': [629], 'stage': [633], 'C-terminal': [636, 695, 950, 1144, 2704, 2783, 3176, 3356, 3411, 3641, 4048], 'thioester': [637, 659, 696, 778], 'partner,': [641, 701], 'selectively': [642], 'cleavable': [643], 'thiols.': [645], 'second': [647, 3336, 4025], 'peptide': [648, 664], 'carrying': [649], 'N-terminal': [651, 945, 1191, 2695, 3172, 3330, 3379, 3627, 3637, 4075], 'cysteine': [652, 683], 'acts': [653], 'S-nucleophile': [656], 'group,': [660], 'forming': [661], 'bond': [665, 2033, 2062], 'after': [666, 1549, 1652, 2534, 2564, 2593, 3178, 3377, 3861, 4546], 'S–N': [667], 'rearrangement.': [668], 'However,': [669, 3441], 'disadvantage': [674], 'it': [676, 2800, 3541, 3618, 4497], 'requires': [677], 'nucleophilic': [679], 'thiol': [680], 'group': [681, 779], 'N': [686, 1057, 1136, 2036, 2700, 2727, 2944, 2987, 3144], 'terminus': [687, 2692, 2701, 2988, 2994], 'partner': [690], 'modification': [697], 'whose': [702], 'formation': [703, 3533], 'catalyzed': [705], '(5Muir': [709], 'Scholar,': [729, 793, 808, 980, 2426, 2844, 2871, 3024, 3103, 3124, 3215, 3236, 4326, 4341, 4408], 'can': [749, 923, 2713, 2807, 2969], 'be': [750, 863, 924, 1023, 2715, 2808, 2970, 3414, 3434, 3528, 3543, 3935, 4040, 4262, 4294, 4499, 4522], 'used': [751, 925, 2006], 'make': [753], 'peptides': [755, 874, 890, 931], 'proteins,': [757], 'intramolecular': [760], 'always': [763, 4304], 'compete': [766], 'intermolecular': [769], 'reactions': [770], 'polymerization': [773], 'hydrolysis': [775], '8Camarero': [794, 4327], 'J.A.': [795, 4328], 'Muir': [796, 4329], '121:': [803, 4336], '5597-5598Crossref': [804, 4337], '(122)': [806, 4339], '9Evans': [809, 4342], 'Biol.': [817, 1003, 1892, 2116, 2884, 3095, 3207, 3566, 4350, 4431], '274:': [820, 4353], '18359-18363Abstract': [821, 4354], 'Full': [822, 824, 1008, 1010, 3571, 3573, 4355, 4357, 4436, 4438], 'Text': [823, 825, 1009, 1011, 3572, 3574, 4356, 4358, 4437, 4439], 'PDF': [826, 1012, 3575, 4359, 4440], '(119)': [829, 4362], 'Scholar),': [831, 1288, 3040], 'which': [832, 1390, 1500, 3889, 3933, 4202, 4303, 4651], 'reduce': [833], 'efficiency': [836, 4492], 'complicate': [838], 'purification': [840, 1717, 3896, 4028], 'An': [856], 'without': [860, 1455, 3650, 4618], 'by-products': [861], 'would': [862, 3047, 4629], 'importance': [865], 'large-scale': [868], 'production': [869], 'vivo.': [876, 2962], 'provide': [880], 'possibility': [882], 'creating': [884, 1039], 'large': [886, 1403, 3953], 'library': [887], 'functional': [896, 1080, 3051, 3531, 4066, 4505], 'selections': [897], 'biophysical': [902], 'characterization': [903], 'Two': [907, 3795], 'independent': [908], 'groups': [909, 1991], 'demonstrated': [911, 2803], 'naturally': [914, 936, 1029, 4372, 4516], 'split': [915, 1030, 2810, 3598, 4373, 4510, 4517], 'DnaE': [917, 4376], 'fromSynechocystis': [918], 'sp.': [919], 'PCC6803': [920], '(Ssp': [921], 'DnaE)': [922], 'vivoby': [933], 'arranging': [934], 'separated': [937, 1813], 'two': [938, 2710, 3825], 'fragments': [939, 3623, 4036], 'intein,': [942, 1031, 4067, 4518], 'DnaEN': [943], '(the': [944, 949], 'part)': [946], 'DnaEC': [948], 'part),': [951], 'order': [954], 'DnaEC-extein-DnaEN': [955], '10Evans': [981, 4409], 'Martin': [984, 4412], 'Kolly': [986, 4414], 'R.': [987, 1885, 1971, 3030, 3088, 3200, 4415], 'Panne': [988, 4416], 'Sun': [990, 4418], 'L.': [991, 995, 1339, 4419, 4423], 'Ghosh': [992, 4420], 'I.': [993, 4421], 'Chen': [994, 4422], 'Liu': [998, 4426], 'X.Q.': [999, 4427], '275:': [1006, 4434], '9091-9094Abstract': [1007, 4435], '(169)': [1015, 4443], 'theory,': [1019], 'strategy': [1021], 'should': [1022, 1068, 3413, 4261], 'feasible': [1024], 'not': [1025, 3149, 3463, 3847, 3925], 'only': [1026, 1661, 4156, 4526], 'any': [1035, 3651], 'artificially': [1038, 1943, 2809, 3597, 4509], 'similar': [1041, 3290, 4565], 'arrangement.': [1042], 'corresponds': [1044, 2722, 4008], 'essentially': [1045], 'introduces': [1055], 'C': [1059, 1138, 2038, 2517, 2691, 2729, 2946, 2993, 3146], 'end': [1066, 2967], 'products': [1067, 1317], 'include': [1069], 'if': [1073], 'folds': [1077], '(see': [1082, 1590, 1668, 1740, 2409, 2452, 4253], 'Fig.': [1083, 1591, 1669, 2097, 2453, 2515, 3360, 3516], '1).': [1084], 'report,': [1087], 'we': [1088, 2791, 2901, 3442, 3503, 3582, 3923], 'tested': [1090], 'our': [1091, 1119, 2789, 3267], 'idea': [1092], 'within': [1101, 1929, 2716], 'domains': [1103, 2734, 3494, 3678, 4050], 'using': [1104, 1178, 1224, 1837, 1854, 1911, 2375, 4370], 'PI-PfuI1': [1107], 'furiosus.': [1110], 'We': [1111, 4239, 4446, 4598], 'chose': [1112, 2792, 2902], 'target': [1121, 2895, 4552], 'because': [1122, 2799, 4055], 'useful': [1128], 'studies': [1131], 'roles': [1134, 2942], 'cellular': [1141, 2953], 'environments.': [1142], 'part': [1145, 1192, 3345], '(PI-PfuIC,': [1146], 'residues': [1147, 1194, 2816, 3157, 3260, 3392, 3420, 3432, 3450], '161–454)': [1148], 'PI-PfuI': [1152, 1410, 2795], 'amplified': [1154, 1200], 'pHisInα': [1156, 1237], '(11Otomo': [1157, 1203, 2824], 'T.': [1158, 1164, 1204, 1210, 1245, 2825, 2831, 2846, 2848], 'Teruya': [1159, 1205, 2826], 'K.': [1160, 1162, 1206, 1208, 1975, 2113, 2417, 2827, 2829, 2854, 2873, 2879, 2881, 3109, 3221], 'Uegaki': [1161, 1207, 2828, 2853], 'Yamazaki': [1163, 1209, 1246, 2830], 'Kyogoku': [1165, 1211, 2832, 2851], 'Y.': [1166, 1212, 2833, 2852, 2858, 2875], 'Biomol.': [1168, 1214, 2835], 'NMR.': [1169, 1215, 2836], '14:': [1171, 1217, 1282, 1623, 1980, 2838, 2924, 3135, 3247, 4144], '105-114Crossref': [1172, 1218, 2839], '(84)': [1175, 1221, 2842], 'oligonucleotides': [1179, 1225], '5′-CTTTAAGAAGGAGATATA-3′': [1180], '5′-CTCAGTAAGAGATTTTTTTCTAGATCCGGTGTTGTGGACG-3′,': [1182], 'followed': [1183, 1229, 1464, 1715, 1768, 2455, 3759, 4593], 'digestion': [1185, 1231, 1682, 3863], 'NcoI': [1187, 1361], 'XbaI.': [1189], '(PI-PfuIN,': [1193], '1–160)': [1195], 'pCBDαIn': [1202, 1239], '5′-GTTCGGTACCGGATGCATAGACGGAAAGG-3′': [1226], '5′-TGGAAGCTTACTTAACATGTGAGTGG-3′,': [1228], 'withKpnI': [1232], 'HindIII.': [1234], 'plasmids': [1236], 'were': [1240, 1318, 1414, 1484, 1505, 1521, 1584, 1759, 1812, 1821, 1942, 1992, 2005, 2088, 2102, 2132, 2142, 2161, 2171, 2181, 2196, 2223, 2298, 2397, 2532, 2617, 2646, 3696, 3713, 3775, 3803, 3846, 3974, 3986, 4071, 4233], 'kindly': [1241], 'provided': [1242], 'Dr.': [1244], '(Osaka': [1247], 'University).': [1248], 'gene': [1250, 3312], 'GFP,': [1252], 'DNA': [1255], 'shuffling': [1256], '(GFPuv,': [1257], 'Cycle3': [1261, 2911], 'mutant)': [1262, 2912], 'bearing': [1264], 'mutations': [1266], 'F99S,': [1267], 'M153T,': [1268], 'V163A': [1270], '(12Crameri': [1271, 2913], 'Whitehorn': [1273, 2915], 'E.A.': [1274, 2916], 'Tate': [1275, 2917], 'Stemmer': [1277, 2919], 'W.P.': [1278, 2920], 'Nat.': [1279, 1890, 2921, 3093, 3132, 3205, 3244], '1996;': [1281, 1979, 2923, 3117, 3134, 3229, 3246], '315-319Crossref': [1283, 2925], '(1060)': [1286, 2928], 'obtained': [1290, 1907, 2074, 2618, 4599], 'pBAD/AC2': [1292], '(PCR)': [1297], 'amplification': [1298], 'primers': [1300], '5′-CCTCTAGACATCATCACCACCATCACTCTAGAAAAGGAGAAGAACTYTTC-3′': [1301], '5′-TAGGTACCGCGTGGCACCAACCCAGCAGCWGTTAC-3′.': [1303], 'product': [1308, 2968, 4015], 'digested': [1310, 4605], 'XbaI': [1312], 'andKpnI.': [1313], 'These': [1314, 2709], 'three': [1315, 2082, 2772, 3622], 'PCR': [1316], 'then': [1319, 1441, 1782, 1803, 1822, 4069], 'ligated': [1320], 'under': [1326, 3691, 3703, 4059], 'control': [1328], 'T7': [1331], 'promoter': [1332], 'expression': [1335, 1462], 'vector': [1336], 'pTFT74': [1337], '(13Ge': [1338], 'Knappik': [1340], 'Pack': [1342], 'P.': [1343, 2109, 2797], 'Freund': [1344], 'C.': [1345, 2111], 'Borrebaeck': [1348], 'C.A.K.': [1349], 'Antibody': [1350], 'Engineering.': [1351], 'Oxford': [1352], 'University': [1353], 'Press,': [1354], 'New': [1355], 'York1995:': [1356], '229-266Google': [1357], 'between': [1359, 1935, 2034, 2815, 3156, 3328, 3350, 3391, 3473], 'HindIII': [1363], 'sites': [1364], 'stepwise': [1367], 'manner,': [1368], 'yielding': [1369], 'plasmid': [1371, 1383, 1757], 'pIWT5563his.': [1372, 1384], 'For': [1373, 1689, 2048, 2268, 2788], 'expression,Escherichia': [1375], 'coli': [1376, 1393, 1754], 'strain': [1377], 'BL21-CodonPlus(DE3)-RIL': [1378], '(Stratagene)': [1379], 'transformed': [1381], 'strain,': [1389], 'overexpresses': [1391], 'tRNAs': [1394], 'rare': [1396, 1406], 'codons,': [1397], 'necessary': [1399], 'number': [1404], 'codons': [1407], 'gene.': [1411], 'cells': [1413, 1483, 1520, 1755, 3461, 3695], 'grown': [1415, 1760], '30': [1417, 1430, 1530], '°C': [1418, 1794, 2175, 3730, 3764], '1': [1420, 1556, 2155, 2236, 2240, 2250, 2254, 2266, 2313, 2316, 2748, 3723, 3756, 4175], 'liter': [1421, 4176], 'LB': [1423, 1766, 3684], 'medium': [1424, 3685], 'containing': [1425, 1756, 2302, 2337, 3067, 3643, 3722, 3755], '50': [1426, 1508, 1566, 2151, 2226, 2245, 2303, 2309], 'μg/ml': [1427, 1431, 2347, 2469], 'ampicillin': [1428], 'chloramphenicol': [1432], 'untilA550': [1433], '∼': [1434], '0.5': [1435, 2346, 2585], 'reached.': [1437], 'slow': [1445, 2505], 'incubation': [1448, 1477, 1524], 'room': [1450, 1713, 1763, 2407, 2450, 3687], 'temperature': [1451, 1764, 2408, 2451, 3688], '(∼25': [1452], '°C)': [1453], 'overnight': [1454, 1761], 'induction,': [1456], 'taking': [1457], 'advantage': [1458, 4296], 'leaky': [1461], 'vector,': [1463], 'addition': [1466, 1653], 'isopropyl-β-d-thiogalactopyranoside': [1468], 'final': [1471, 1773, 2258, 2342, 2464, 2582], 'concentration': [1472, 1774, 2260, 2320, 2344, 2466, 2583], '0.1': [1474, 1776, 2191], 'mm': [1475, 1509, 1517, 1567, 1573, 1577, 1588, 2146, 2152, 2156, 2227, 2237, 2241, 2246, 2251, 2255, 2304, 2310, 2314, 3724, 3745, 3757, 3978], 'another': [1479], '6': [1480, 3001, 3431], 'h.': [1481], 'harvested': [1485, 3697], 'centrifugation': [1487, 1550], '4000': [1489], '×g': [1490], '10': [1492, 1695, 2177, 3732], 'min': [1493, 1531, 1797, 3733, 3767], '4': [1495, 1559, 1779, 3900], '°C.': [1496, 1560, 2206, 2350], 'cell': [1498, 1538, 1810, 3711, 3773, 3850, 3958, 4456], 'pellets,': [1499], 'showed': [1501, 3699, 3833], 'bright': [1502, 3700, 4108, 4184], 'fluorescence,': [1504], 'resuspended': [1506, 1804], 'sodium': [1510, 1568, 2311], 'phosphate': [1511, 1569, 2148, 2229, 2306], 'buffer': [1512, 1789, 3721, 3754], '(pH': [1513, 1570, 2149, 2230, 2248, 2307, 2572, 3747], '8.0)': [1514, 3748], '300': [1516, 1572, 1587, 3977], 'NaCl.': [1518], 'lysed': [1522], 'lysozyme': [1526], '(1': [1527, 3955], 'mg/ml)': [1528], 'sonication': [1534, 3742, 3829], 'ice.': [1536], 'lysate': [1539, 3959], 'loaded': [1541], '10-ml': [1544, 1720], 'Ni2+-NTA': [1545, 1721, 3970, 4103, 4284], 'column': [1546, 1562, 4104, 4285], '(QIAGEN': [1547, 1841], 'Inc.)': [1548, 1842], '20,000': [1552], '×': [1553], 'g': [1554], 'h': [1557, 1711, 2405, 2566, 2595], 'washed': [1564], '8.0),': [1571, 2150], 'NaCl,': [1574, 2153], '20': [1576, 2594, 3744], 'imidazole.': [1578], 'hexahistidine-tagged': [1580], 'including': [1582, 3451], 'GFPuv': [1583, 1678, 1698, 1739, 2563, 2908, 3258, 3282, 3311, 3353, 3370, 3476, 3490, 3508, 3655, 3680, 4022, 4121, 4252, 4464, 4575, 4588, 4616], 'eluted': [1585, 1597, 3975, 3999], 'imidazole': [1589, 3979], '3': [1592, 1670, 2404, 2565, 3261, 3735, 3769, 3853, 3981, 4090, 4164], 'B,': [1593, 1671, 1742, 2588, 2604, 3982, 4165], 'lane': [1594, 1672, 1743, 3983, 4166], '2).': [1595, 3984], 'sample': [1598, 1632, 4617], 'further': [1600], 'purified': [1601, 1728, 3919, 4072, 4275, 4469, 4615, 4639], 'organic': [1603, 1650, 4129, 4289], 'extraction': [1604, 4131, 4290], 'described': [1606, 3690], 'Yakhnin': [1608], 'et': [1609], 'al.': [1610], '(14Yakhnin': [1611, 4132], 'A.V.': [1612, 4133], 'Vinokurov': [1613, 4134], 'L.M.': [1614, 4135], 'Surin': [1615, 4136], 'A.K.': [1616, 4137], 'Alakhov': [1617, 4138], 'Y.B.': [1618, 4139], 'Expression': [1620, 4141], 'Purif.': [1621, 4142], '382-386Crossref': [1624, 4145], '(88)': [1627, 4148], 'Briefly,': [1630], 'saturated': [1634], 'ammonium': [1636], 'sulfate': [1637], 'extracted': [1639, 1657], 'ethanol.': [1641], 'aqueous': [1643, 1658], 'subsequently': [1646], 'removed': [1647], '1-butanol.': [1655], 'fraction': [1659, 2509, 2632, 3965, 4125, 4379], 'contained': [1660], 'single': [1663, 1724, 2362, 4158, 4244], 'band': [1664, 1725, 3857, 3882, 3911, 4002, 4083, 4159, 4245], '∼28': [1666], 'kDa': [1667, 3802, 3996, 4018, 4162], '3).': [1673, 3945], 'thrombin.': [1688], 'purpose,': [1691], '1.5': [1692], 'ml': [1693], 'μm': [1696], 'incubated': [1700], '0.2': [1702], 'units': [1703], 'thrombin': [1705, 3341, 3668, 3862, 4549, 4569, 4619], '(Roche': [1706, 1847], 'Molecular': [1707], 'Biochemicals)': [1708], '2.5': [1710], 'temperature,': [1714], 'column.': [1722, 3971], 'migrated': [1731, 3858, 4641], 'more': [1732, 3415, 3859, 4500, 4523], 'slowly': [1733, 3860], 'than': [1734, 3416, 4512, 4643], 'Fig.3': [1741], '4).': [1744], 'To': [1745, 4119, 4471], 'examine': [1746], 'splicing': [1748, 2682, 2764, 4474, 4491], 'vivo,': [1751], 'pIWT5563his': [1758], 'medium,': [1767], 'induction': [1770], 'mmisopropyl-β-d-thiogalactopyranoside': [1777], 'h,': [1780], 'either': [1783, 3715], 'directly': [1784], 'mixed': [1785], 'SDS': [1787, 1806, 3719, 3752], 'loading': [1788, 1807, 3720, 3753], 'boiled': [1791], '95': [1793, 3729, 3763], '5': [1796, 1930, 3429, 3766, 4093], 'alternatively': [1799], 'first': [1800, 2398, 3374], 'sonicated': [1801], 'buffer.': [1808, 2462], 'lysates': [1811, 3712, 3774], '12%': [1815], 'SDS-polyacrylamide': [1816, 4648], 'gel': [1817, 4649], 'electrophoresis.': [1818], 'transferred': [1823], 'polyvinylidene': [1826], 'difluoride': [1827], 'membrane': [1828], 'Western': [1830, 1833, 3778, 3839, 3913], 'blot': [1831], 'analysis.': [1832], 'blotting': [1834, 3779], 'performed': [1836, 2089, 2103, 2299], 'Tetra·His': [1839], 'AntibodyTM': [1840], 'anti-GFP': [1845, 3782, 3815], 'antibody': [1846, 3783, 3792, 3809], 'Diagnostics)': [1848], 'visualized': [1850], 'colorimetric': [1852], 'detection': [1853], 'alkaline': [1855], 'phosphatase-conjugated': [1856], 'anti-mouse': [1857], 'IgG.': [1858], 'modeled': [1865, 1904, 3368], 'based': [1866, 1920, 1949], 'crystal': [1869, 1918, 1964, 2003, 3054, 3183], '(Protein': [1873], 'Data': [1874], 'Bank': [1875], 'code': [1876], '1EMC)': [1877], '(15Palm': [1878, 3081, 3193], 'G.J.': [1879, 3082, 3194], 'Zdanov': [1880, 3083, 3195], 'Gaitanaris': [1882, 3085, 3197], 'G.A.': [1883, 3086, 3198], 'Stauber': [1884, 3087, 3199], 'Pavlakis': [1886, 3089, 3201], 'G.N.': [1887, 3090, 3131, 3202, 3243], 'Wlodawer': [1888, 3091, 3203], 'Struct.': [1891, 3094, 3206], '1997;': [1893, 2117, 2432, 3096, 3208, 3568], '4:': [1894, 3097, 3209], '361-365Crossref': [1895, 3098, 3210], '(234)': [1898, 3101, 3213], 'follows.': [1902], 'simulated': [1909, 2085], 'annealing': [1910, 2086], 'artificial': [1912], 'distance': [1913, 1933, 1947, 2011, 2046, 2072, 3390], 'constraints': [1914, 1934, 1948, 2012], 'created': [1915, 1944], 'hydrogen': [1922, 1936, 1959, 1998, 2080], 'bonds': [1923, 1999], 'proton-proton': [1927, 1951, 2077], 'distances': [1928, 1952, 2078], 'Å.': [1931, 3398], 'atoms': [1937, 1960], 'model': [1940, 2093], 'building': [1941], 'upper': [1946, 2008, 2043], '<5': [1954], 'Å': [1955, 3155], 'all': [1957, 2262, 2471], 'calculated': [1961], 'program': [1968, 2106], 'MOLMOL': [1969], '(16Koradi': [1970], 'Billeter': [1972], 'Wüthrich': [1974, 2112], 'Mol.': [1977, 2115, 2883], 'Graphics.': [1978], '52-55Crossref': [1981], '(6490)': [1983], 'methyl': [1988], 'methylene': [1990], 'treated': [1993, 2021, 4590], 'pseudo': [1995], 'atoms.': [1996], 'lower': [2010, 2045], 'calculation.': [2016, 2030], 'chromophore': [2018, 2355, 2477, 3069], 'original': [2024, 2726, 3255], 'unmodified': [2025], 'during': [2028, 3849], 'covalent': [2032, 2061], 'constraints.': [2047], 'linker': [2050, 2999, 3327, 3349, 3400, 3427, 3472], 'connecting': [2051, 2063, 3045], 'both': [2052, 2076, 3489, 4070], 'termini,': [2053], 'constraint': [2057], 'applied': [2058, 3967], 'besides': [2059], 'termini.': [2065], 'With': [2066], 'total': [2068, 2640, 3607], '9188': [2071], 'constraints,': [2073], 'bonds,': [2081], 'rounds': [2083], 'calculation': [2087], 'obtain': [2091], 'depicted': [2095, 3384], '2': [2098, 3158, 3270, 3314, 3361, 3393, 3421, 3517, 3737, 3943, 4585, 4636], 'B.': [2099], 'calculations': [2101], 'DYANA': [2107], '(17Güntert': [2108], 'Mumenthaler': [2110], '273:': [2118, 3118, 3230], '283-298Crossref': [2119], '(2555)': [2122], 'SGI': [2127], 'Octane.': [2128], 'CD': [2129], 'wavelength': [2130], 'scans': [2131, 2222], 'recorded': [2133, 2172, 2197, 2285], 'Jasco': [2136], 'J-715': [2137], 'spectropolarimeter.': [2138], 'samples': [2141, 2216, 2264, 3826], '100': [2145], 'potassium': [2147, 2228, 2305], 'EDTA;': [2157], 'concentrations': [2160, 2529], '0.12': [2162], 'mg/ml': [2163, 2167], '(linear)': [2164], '0.15': [2166], '(circular).': [2168], 'spectra': [2170, 2195], '25': [2174, 2205, 2349], 'scans.': [2178], 'data': [2180], 'normalized': [2182], 'molar': [2184], 'ellipticity': [2185], 'path': [2188, 2211], 'length': [2189, 2212, 3608], 'cm.': [2192], 'fluorescence': [2194, 2273, 2356, 2387, 2478, 2641, 3458, 3552, 3702], 'PTI': [2200], 'Alpha': [2201], 'Scan': [2202], 'spectrofluorometer': [2203], 'cuvette': [2208], '1-cm': [2210], 'used.': [2214], 'emission': [2219, 2282], 'excitation': [2221, 2270, 2288, 2627], '6.5,': [2231], '7,': [2232], '7.5,': [2233], '8),': [2235, 2308], 'EDTA,': [2238, 2315], 'DTT': [2242, 3725], 'Tris': [2247, 3746], '8.5),': [2249], 'EDTA': [2252], 'DTT.': [2256], 'μg/ml.': [2267], 'spectrum,': [2271], 'intensity': [2274, 2357, 2479, 2622], 'monitored': [2276, 2972], '508': [2278, 2624], 'nm,': [2279], 'whereas': [2280, 3284], 'spectrum': [2283], '397': [2290, 2629, 4193], 'nm.': [2291, 2630], 'kinetic': [2293, 2370, 2498, 2536], 'unfolding': [2294, 2325, 2335], 'refolding': [2296, 2393, 2461, 2554], 'experiments': [2297], 'solutions': [2301], 'chloride,': [2312], 'mmDTT,': [2317], 'GdnHCl.': [2322, 2587], 'experiments,': [2326, 2394], 'solution': [2329, 2336, 4181], 'diluted': [2331], '1:320': [2332], 'GdnHCl,': [2338], 'resulting': [2339, 3405], 'change': [2352, 2474], 'fitted': [2359, 2481, 2526, 2647], 'exponential,A·exp(−k·t),': [2363], 'where': [2364], 'A,k,': [2365], 't': [2367], 'amplitude,': [2369], 'constant,': [2371], 'time,': [2373, 2739], 'respectively,': [2374, 4054], 'SigmaPlot': [2376], 'software': [2377], '(SPSS': [2378], 'Inc.,': [2379], 'Chicago,': [2380], 'IL).': [2381], 'equation': [2383], 'assumes': [2384], 'infinite': [2389], 'time.': [2390, 2643], '6m': [2401], 'GdnHCl': [2402, 2445, 2531, 2571], 'Fig.6': [2410], 'B)': [2411, 2523, 3080], '(18Fukuda': [2412], 'Arai': [2414], 'Kuwajima': [2416], 'Biochemistry.': [2418, 2431], '39:': [2420], '12025-12032Crossref': [2421], '19Reid': [2427], 'B.G.': [2428], 'Flynn': [2429], 'G.C.': [2430], '36:': [2433], '6786-6791Crossref': [2434], '(363)': [2437], '7': [2443, 2516, 2599], 'm': [2444, 2586], 'times': [2448], '7),': [2454], '1:25–40': [2457], 'dilution': [2458, 2579], '∼0.5': [2468], 'measurements.': [2472], 'double': [2484], 'exponential,A·(1': [2485], '−': [2486, 2489], 'exp(−k1·t))': [2487], '+B·(1': [2488], 'exp(−k2·t))': [2490], '+': [2491, 2522], 'C,': [2492, 2631], 'wherek1': [2493], 'k2': [2495], 'constants': [2499], 'phases,': [2506], 'respectively.': [2507, 2614], 'determined': [2519, 2533], 'calculatingA/(A': [2521], 'values.': [2527], 'measurements': [2537], 'measuring': [2539], 'refractive': [2541], 'index.Figure': [2542], '7Refolding': [2543], 'kinetics': [2544, 2555], 'forms': [2550, 2561, 4237, 4312], 'GFPuv.': [2552, 3339, 3823, 4118], 'A,': [2553, 2591, 3785, 3898, 3941, 4091], '8.0).': [2573], 'started': [2577], 'same': [2589, 2738, 3166, 3835, 3867], 'mGdnHCl.': [2600], 'thin': [2605], 'andthick': [2606], 'lines': [2607], 'represent': [2608], 'forms,': [2613], 'traces': [2616], 'following': [2620, 4573], 'nm': [2625, 4194, 4199], 'recovered': [2639], 'versusdenaturation': [2642], 'points': [2645], 'exponential': [2650], 'function.': [2651], '▪,': [2652], 'form;': [2654], '■,': [2655], 'form.View': [2657], 'Large': [2658], 'Image': [2659], 'Figure': [2660], 'ViewerDownload': [2661], 'Hi-res': [2662], 'image': [2663], 'Download': [2664], '(PPT)': [2665], 'Our': [2666], 'concept': [2667], 'produce': [2669], 'simple.': [2676], 'conventional': [2678], 'activity': [2683], 'connect': [2689, 3419], 'fragment': [2696, 2705, 3177, 3628, 3642], '(N-extein)': [2697], '(C-extein)': [2706], '(Fig.1': [2707], 'A).': [2708, 3315, 3854, 4586, 4637], 'interacting': [2711], 'extein': [2718, 2733, 2778], 'Topologically,': [2720], 'joining': [2724, 2985], 'domain': [2746, 3332, 3358, 3381], '(Fig.': [2747, 3269, 3313, 3734, 3852, 3980, 4089, 4163, 4584, 4635], 'B).': [2749], 'nothing': [2752], 'protein.': [2760, 4013], 'After': [2761, 3946, 4151], 'reaction,': [2765], 'gives': [2769], 'rise': [2770], 'fragments,': [2774], 'viz.': [2775], 'N-': [2781, 3409, 4046], 'parts': [2784], 'intein.': [2787], 'study,': [2790, 3502], 'furiosus': [2798], 'extended': [2813, 3426], 'loop': [2814, 3046], '160': [2817], '161': [2819], 'performtrans-ligations': [2821], '20Yamazaki': [2845], 'Otomo': [2847], 'Oda': [2849], 'N.': [2850, 2856, 3558], 'Ito': [2855], 'Ishino': [2857, 2874], 'Nakamura': [2859], '120:': [2866], '5591-5592Crossref': [2867], '(194)': [2869], '21Ichiyanagi': [2872], 'Ariyoshi': [2876], 'Komori': [2878], 'Morikawa': [2880], '300:': [2886], '889-901Crossref': [2887], '(97)': [2890], 'As': [2893], '(i.e.': [2898], 'extein),': [2900], '(also': [2909], 'since': [2931], 'constitute': [2935, 3324], 'Moreover,': [2963], 'properly': [2965, 4116, 4484], 'folded': [2966, 4117, 4485], 'conveniently': [2971], 'its': [2974, 3320], 'fluorescence.': [2976], 'Furthermore,': [2977, 4231], 'successfully': [2981], 'circularly': [2982], 'permuted': [2983], '(residue': [2989, 2995], '1)': [2990], '238)': [2996], '(22Baird': [3004], 'G.S.': [3005], 'Zacharias': [3006], 'D.A.': [3007], 'Tsien': [3008, 3112, 3224], 'R.Y.': [3009, 3113, 3225], '11241-11246Crossref': [3019], '(740)': [3022], '23Topell': [3025], 'Hennecke': [3027], 'Glockshuber': [3029], '457:': [3034], '283-289Crossref': [3035], '(110)': [3038, 3578], 'suggesting': [3041, 3879], 'likely': [3048, 4038], 'GFP.': [3052], 'indicates': [3058], 'cylinder-like': [3064], 'β-barrel': [3065, 3078], 'distorted': [3072], 'central': [3073], 'helix': [3074], 'surrounded': [3075], '(Fig.2': [3079], '24Ormö': [3104, 3216], 'Cubitt': [3106, 3218], 'A.B.': [3107, 3219], 'Kallio': [3108, 3220], 'Gross': [3110, 3222], 'L.A.': [3111, 3223], 'Remington': [3114, 3226], 'Science.': [3116, 3228], '1392-1395Crossref': [3119, 3231], '(1927)': [3122, 3234], '25Yang': [3125, 3237], 'F.': [3126, 3238], 'Moss': [3127, 3239], 'L.G.': [3128, 3240], 'Phillips': [3129, 3241], '1246-1251Crossref': [3136, 3248], '(1294)': [3139, 3251], 'visible': [3143], 'very': [3151], 'close': [3152], 'proximity': [3153], '(∼19': [3154], '229),': [3160], 'located': [3163], 'side': [3167], 'cylinder.': [3170], 'residue': [3173, 3179, 3299, 3337, 3375, 3522, 3585], '229': [3180, 3395, 3523], 'structures': [3184], 'often': [3188], 'invisible': [3189], 'probably': [3191, 3467, 4494], 'disordered': [3192], '228': [3263], 'unchanged': [3265], 'construct': [3268, 3509], 'A,underlined),': [3271], 'hexahistidine': [3274, 3317, 3453, 3638, 3664, 4076], 'tag': [3275, 3318, 3454, 3665, 3791, 3808, 4077, 4087], 'added': [3277], 'front': [3279, 4579], 'gene,': [3283], 'thrombin-specific': [3286, 3293, 3591], 'proteolytic': [3287, 3592, 4562], '(LVPRGT,': [3289, 4564], 'LVPRGS,': [3295], 'Thr': [3298], 'being': [3300, 3477], 'generate': [3303], 'KpnI': [3305], 'site)': [3306], 'behind': [3309], 'flanking': [3321], 'together': [3323, 3600], '11-residue': [3326], '(PI-PfuIC)': [3333, 3382], 'recognition': [3342, 4570], '6-residue': [3348], 'Leu229': [3351], '(PI-PfuIN).': [3359], 'B': [3362], 'provides': [3363], 'overview': [3365], 'structure,': [3371, 4489], '(threonine)': [3376], 'ball-and-stick': [3387], 'model.': [3388], '∼19': [3397], '17': [3402], 'acids,': [3404, 3632, 3647, 3658], 'spliced': [3408, 4042, 4528], 'extension,': [3412], 'sufficient': [3417], '229.': [3423], 'Even': [3424, 4365], 'sufficiently': [3435], 'long': [3436], 'span': [3438], 'distance.': [3440], 'removal': [3447], '8': [3449], 'resulted': [3455, 4105], 'weaker': [3457], '(data': [3462], 'shown).': [3464], 'consequence': [3469], 'PI-PfuIC': [3474, 3629, 4079], 'too': [3478], 'short': [3479], 'allow': [3481], 'efficient': [3482], 'correct': [3486, 4488], 'some': [3497], 'steric': [3498], 'hindrance.': [3499], 'focused': [3505], '245': [3511], 'presence': [3520, 4114], '(isoleucine)': [3524], 'essential': [3529], 'truncation': [3538], 'experiment,': [3539], 'could': [3542, 4057, 4293], 'replaced': [3544, 3583], 'leucine': [3546, 3587], 'measurable': [3549], 'loss': [3550], '(26Li': [3553], 'X.': [3554, 3560], 'Zhang': [3555], 'G.': [3556], 'Ngo': [3557], 'Zhao': [3559], 'Kain': [3561], 'S.R.': [3562], 'Huang': [3563], 'C.C.': [3564], '272:': [3569], '28545-28549Abstract': [3570], 'Therefore,': [3581], 'introduce': [3589], 'site.': [3593, 3670], 'GFPuv-extein': [3594], 'inteins': [3599], '720': [3610], '(83': [3613], 'kDa).': [3614], 'Upon': [3615], 'splicing,': [3617], 'released:': [3625], '(315': [3630], '37': [3633], 'kDa)': [3634, 3649, 3660], 'tag,': [3639, 3652], 'PI-PfuIN': [3644, 4051, 4582], '(160': [3645], '18.7': [3648], '(245': [3656], '27.5': [3659], 'internal': [3663], 'cleavage': [3669], 'consisting': [3674], '"Experimental': [3692], 'Procedures."': [3693], 'UV': [3705], 'lamp': [3706], '365-nm': [3708], 'wavelength.': [3709], 'Total': [3710], 'resuspension': [3717, 3750], 'boiling': [3727, 3761, 3832], 'A,lanes': [3736], '5)': [3739], 'DTT,': [3758], '(lanes': [3768, 3793], '6).': [3771], 'analyzed': [3776], '(Fig.3': [3784, 3897, 3940], 'lanes': [3786, 3899, 3942, 4092], '2–4)': [3787], 'anti-His': [3790, 3807, 4086], '5–9).': [3794], 'stronger': [3796], 'bands': [3797, 3845, 3989], '∼80': [3799], '28': [3801, 4017, 4161], 'detected': [3804, 4612], 'antibody,': [3816], 'corresponding': [3817], 'pattern': [3836], 'blots,': [3840], 'indicating': [3841, 4112], '∼30-kDa': [3844], 'disruption': [3851], '28-kDa': [3856], 'position': [3868, 3917, 4272], 'form,': [3872, 4277], 'explained': [3874], 'detail': [3876], 'below,': [3877], 'strongly': [3878], 'GFPuv,': [3888, 3921], 'present': [3891, 4466], 'already': [3894], 'before': [3895], '7).': [3902], 'noteworthy': [3905], 'detectable': [3910, 4235, 4268], 'blots': [3914], 'do': [3924], 'know': [3926], 'nature': [3928], '∼33-kDa': [3931], 'band,': [3932], 'proteolytically': [3937], 'truncated': [3938], 'scale': [3954], 'liter),': [3956], 'centrifuged,': [3961], 'soluble': [3964], 'Bound': [3972], 'four': [3987], 'major': [3988], '80,': [3991], '35,': [3992], '28,': [3993], '19': [3995], 'fraction.': [4000, 4291, 4470], 'highest': [4005], 'molecular': [4006], 'mass': [4007, 4030, 4541], 'unspliced': [4011, 4450], 'identified': [4020], 'step': [4026], 'spectrometry.': [4031], '35-': [4033], '19-kDa': [4035], 'noncovalently': [4044], 'associated': [4045], 'PI-PfuIC,': [4053], 'they': [4056], 'associate': [4058], 'nondenaturing': [4060], 'conditions': [4061], 'whole': [4065], 'via': [4073], '(cf.': [4080], '35-kDa': [4082], 'blot)': [4088], 'and6).': [4094], 'worth': [4097], 'mentioning': [4098], 'elution': [4100, 4124, 4281], 'fraction,': [4111], 'purify': [4120], 'further,': [4122], 'subjected': [4127], 'solvent': [4130], 'extraction,': [4153], '3),': [4167], 'yield': [4170], '∼3': [4172], 'mg': [4173], 'bacterial': [4178], 'culture.': [4179], 'color,': [4186], 'ratio': [4189], 'absorbance': [4191], '280': [4198], '∼1.2,': [4201], 'good': [4205], 'indication': [4206], 'purity': [4209], '(27Ward': [4212], 'W.W.': [4213], 'Prentice': [4214], 'H.J.': [4215], 'Roth': [4216], 'A.F.': [4217], 'Cody': [4218], 'C.W.': [4219], 'Reeves': [4220], 'S.C.': [4221], 'Photochem.': [4222], 'Photobiol.': [4223], '1982;': [4224], '35:': [4225], '803-808Crossref': [4226], '(223)': [4228], 'polymeric': [4236], 'found.': [4238], '"Confirmation': [4254], 'Cyclization': [4256], 'GFPuv"': [4258], 'below).': [4259], 'emphasized': [4263], 'contaminant': [4269], 'neither': [4278], 'nor': [4286], 'over': [4297], 'methods,': [4302], 'involve': [4305], 'mixture': [4307], 'Ssp': [4375], 'yielded': [4377], 'full-length': [4451], 'extracts,': [4457], 'regenerate': [4472], 'activity,': [4475], 'correctly.': [4482], 'Once': [4483], 'high.': [4495], 'Since': [4496], 'difficult': [4501], 'aggregation-prone,': [4524], 'correctly': [4527], 'molecules': [4529], 'escape': [4531], 'precipitation.': [4532], 'confirmed': [4536], 'electron': [4538], 'spray': [4539], 'ionization': [4540], 'spectrometry': [4542], 'Edman': [4544], 'linearization': [4547], 'cleavage.': [4550], 'designed': [4555], 'specific': [4561], 'normal': [4568], 'LVPRGS)': [4572], 'Purified': [4587], 'thrombin,': [4592], 'sequencing.': [4597], 'GTGTG': [4602], 'protein,': [4607], 'digestion,': [4620], 'confirming': [4621], 'successful.': [4626], 'given': [4631], 'GTGCI': [4634], 'faster': [4642], 'electrophoresis,': [4650], 'circula': [4656]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2011159612', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 9}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 5}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 10}, {'year': 2017, 'cited_by_count': 10}, {'year': 2016, 'cited_by_count': 6}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 8}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-12-09T21:53:23.511776', 'created_date': '2016-06-24'}