Publication Information

{'id': 'https://openalex.org/W1991489953', 'doi': 'https://doi.org/10.1039/a908415k', 'title': 'The application of fluorescence polarisation for the enhanced detection of green fluorescent protein (GFP) in the presence of cellular auto-fluorescence and other green fluorescent compounds', 'display_name': 'The application of fluorescence polarisation for the enhanced detection of green fluorescent protein (GFP) in the presence of cellular auto-fluorescence and other green fluorescent compounds', 'publication_year': 2000, 'publication_date': '2000-01-01', 'ids': {'openalex': 'https://openalex.org/W1991489953', 'doi': 'https://doi.org/10.1039/a908415k', 'mag': '1991489953'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1039/a908415k', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S105746325', 'display_name': 'The Analyst', 'issn_l': '0003-2654', 'issn': ['0003-2654', '1364-5528'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320556', 'host_organization_name': 'Royal Society of Chemistry', 'host_organization_lineage': ['https://openalex.org/P4310320556'], 'host_organization_lineage_names': ['Royal Society of Chemistry'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5010550863', 'display_name': 'Andrew W. Knight', 'orcid': 'https://orcid.org/0000-0002-3792-6363'}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'A. W. Knight', 'raw_affiliation_strings': ['Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5084233981', 'display_name': 'N J Goddard', 'orcid': 'https://orcid.org/0000-0002-5697-7254'}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'N. J. Goddard', 'raw_affiliation_strings': ['Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5057234642', 'display_name': 'Peter R. Fielden', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'P. R. Fielden', 'raw_affiliation_strings': ['Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5017571551', 'display_name': 'A. L. Gregson', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'A. L. Gregson', 'raw_affiliation_strings': ['Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, PO Box 88,Manchester,UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5052068473', 'display_name': 'Nicholas Billinton', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'N. Billinton', 'raw_affiliation_strings': ['Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5010258550', 'display_name': 'Michael Barker', 'orcid': 'https://orcid.org/0000-0001-8962-6234'}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'M. G. Barker', 'raw_affiliation_strings': ['Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK', 'institution_ids': ['https://openalex.org/I28407311']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5085064429', 'display_name': 'Richard M. Walmsley', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'R. M. Walmsley', 'raw_affiliation_strings': ['Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK'], 'affiliations': [{'raw_affiliation_string': 'Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester, UK', 'institution_ids': ['https://openalex.org/I28407311']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.736, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 16, 'citation_normalized_percentile': {'value': 0.76441, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 83, 'max': 84}, 'biblio': {'volume': '125', 'issue': '3', 'first_page': '499', 'last_page': '506'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10540', 'display_name': 'Advanced Fluorescence Microscopy Techniques', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1304', 'display_name': 'Biophysics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12856', 'display_name': 'Transgenic Plants and Applications', 'score': 0.9855, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12561', 'display_name': 'bioluminescence and chemiluminescence research', 'score': 0.9854, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [], 'concepts': [{'id': 'https://openalex.org/C142613039', 'wikidata': 'https://www.wikidata.org/wiki/Q272983', 'display_name': 'Green fluorescent protein', 'level': 3, 'score': 0.9499328}, {'id': 'https://openalex.org/C91881484', 'wikidata': 'https://www.wikidata.org/wiki/Q191807', 'display_name': 'Fluorescence', 'level': 2, 'score': 0.93699896}, {'id': 'https://openalex.org/C62535639', 'wikidata': 'https://www.wikidata.org/wiki/Q15055422', 'display_name': 'Fluorescence in the life sciences', 'level': 4, 'score': 0.5162961}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.50715685}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.5022726}, {'id': 'https://openalex.org/C2779222958', 'wikidata': 'https://www.wikidata.org/wiki/Q45422', 'display_name': 'Yeast', 'level': 2, 'score': 0.43902618}, {'id': 'https://openalex.org/C1342733', 'wikidata': 'https://www.wikidata.org/wiki/Q452891', 'display_name': 'Laser-induced fluorescence', 'level': 3, 'score': 0.31911373}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.20146349}, {'id': 'https://openalex.org/C120665830', 'wikidata': 'https://www.wikidata.org/wiki/Q14620', 'display_name': 'Optics', 'level': 1, 'score': 0.15315321}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.14800993}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.092919886}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.051960528}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1039/a908415k', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S105746325', 'display_name': 'The Analyst', 'issn_l': '0003-2654', 'issn': ['0003-2654', '1364-5528'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320556', 'host_organization_name': 'Royal Society of Chemistry', 'host_organization_lineage': ['https://openalex.org/P4310320556'], 'host_organization_lineage_names': ['Royal Society of Chemistry'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 16, 'referenced_works': ['https://openalex.org/W1993645665', 'https://openalex.org/W2021854512', 'https://openalex.org/W2026359987', 'https://openalex.org/W2026533456', 'https://openalex.org/W2030695915', 'https://openalex.org/W2043668484', 'https://openalex.org/W2050553361', 'https://openalex.org/W2053313583', 'https://openalex.org/W2061104342', 'https://openalex.org/W2063483747', 'https://openalex.org/W2079925707', 'https://openalex.org/W2097994227', 'https://openalex.org/W2130652548', 'https://openalex.org/W2140764310', 'https://openalex.org/W2144259254', 'https://openalex.org/W2468303838'], 'related_works': ['https://openalex.org/W4292395489', 'https://openalex.org/W2384774574', 'https://openalex.org/W2159403775', 'https://openalex.org/W2121967527', 'https://openalex.org/W2097994227', 'https://openalex.org/W2066778047', 'https://openalex.org/W2054530395', 'https://openalex.org/W1991489953', 'https://openalex.org/W1963963052', 'https://openalex.org/W1532211930'], 'abstract_inverted_index': {'The': [0, 54, 81, 101], 'jellyfish': [1], 'green': [2], 'fluorescent': [3, 46], 'protein': [4], '(GFP)': [5], 'is': [6, 69, 106], 'a': [7, 75, 133], 'versatile': [8], 'biological': [9], 'marker': [10], 'and': [11, 44, 71, 78, 85, 96], 'reporter.': [12], 'Here': [13], 'we': [14], 'demonstrate': [15], 'that': [16, 48], 'marked': [17], 'polarisation': [18, 68, 88, 105], 'in': [19, 38, 73, 89, 117], 'GFP': [20, 36, 65, 116, 124], 'fluorescence,': [21], 'upon': [22], 'excitation': [23], 'with': [24, 132], 'plane': [25], 'polarised': [26], 'light,': [27], 'can': [28], 'be': [29], 'exploited': [30], 'to': [31, 60, 108, 122], 'enhance': [32, 109], 'the': [33, 39, 51, 61, 90, 110, 113], 'detection': [34, 63], 'of': [35, 41, 56, 64, 87, 92, 103, 112, 115, 128], 'when': [37], 'presence': [40], 'cellular': [42], 'auto-fluorescence': [43, 91], 'other': [45], 'compounds': [47], 'emit': [49], 'at': [50], 'same': [52], 'wavelength.': [53], 'development': [55], 'flow-though': [57], 'instrumentation': [58], 'dedicated': [59], 'sensitive': [62], 'by': [66], 'fluorescence': [67, 104, 135], 'described,': [70], 'used': [72], 'both': [74], 'continuous': [76], 'flow': [77], 'flow-injection': [79], 'format.': [80], 'intensity,': [82], 'spectral': [83], 'properties': [84], 'extent': [86], 'various': [93], 'yeast': [94, 118], 'strains': [95], 'growth': [97], 'media': [98], 'are': [99], 'investigated.': [100], 'application': [102], 'shown': [107], 'measurement': [111], 'induction': [114], 'cells,': [119], 'genetically': [120], 'modified': [121], 'produce': [123], 'as': [125], 'an': [126], 'indicator': [127], 'DNA': [129], 'damage,': [130], 'compared': [131], 'conventional': [134], 'method.': [136]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1991489953', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 2}], 'updated_date': '2024-12-17T14:37:52.826293', 'created_date': '2016-06-24'}