Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2009783955', 'doi': 'https://doi.org/10.1038/nrg3655', 'title': 'The role of replicates for error mitigation in next-generation sequencing', 'display_name': 'The role of replicates for error mitigation in next-generation sequencing', 'publication_year': 2013, 'publication_date': '2013-12-10', 'ids': {'openalex': 'https://openalex.org/W2009783955', 'doi': 'https://doi.org/10.1038/nrg3655', 'mag': '2009783955', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/24322726', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/4103745'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1038/nrg3655', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S8553189', 'display_name': 'Nature Reviews Genetics', 'issn_l': '1471-0056', 'issn': ['1471-0056', '1471-0064'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319908', 'host_organization_name': 'Nature Portfolio', 'host_organization_lineage': ['https://openalex.org/P4310319908', 'https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Nature Portfolio', 'Springer Nature'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'review', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'green', 'oa_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103745', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5039777028', 'display_name': 'Kimberly Robasky', 'orcid': 'https://orcid.org/0000-0002-0090-8698'}, 'institutions': [], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Kimberly Robasky', 'raw_affiliation_strings': ['Wyss Institute for Biologically Inspired Engineering, Boston, United States'], 'affiliations': [{'raw_affiliation_string': 'Wyss Institute for Biologically Inspired Engineering, Boston, United States', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5074538839', 'display_name': 'Nathan E. Lewis', 'orcid': 'https://orcid.org/0000-0001-7700-3654'}, 'institutions': [{'id': 'https://openalex.org/I36258959', 'display_name': 'University of California, San Diego', 'ror': 'https://ror.org/0168r3w48', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I36258959']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Nathan E. Lewis', 'raw_affiliation_strings': ['University of California, San Diego, San Diego, United States'], 'affiliations': [{'raw_affiliation_string': 'University of California, San Diego, San Diego, United States', 'institution_ids': ['https://openalex.org/I36258959']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5058824230', 'display_name': 'George M. Church', 'orcid': 'https://orcid.org/0000-0001-6232-9969'}, 'institutions': [{'id': 'https://openalex.org/I136199984', 'display_name': 'Harvard University', 'ror': 'https://ror.org/03vek6s52', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I136199984']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'George M. Church', 'raw_affiliation_strings': ['Department of Genetics, Harvard Medical School, and the Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts 02115, USA.,'], 'affiliations': [{'raw_affiliation_string': 'Department of Genetics, Harvard Medical School, and the Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts 02115, USA.,', 'institution_ids': ['https://openalex.org/I136199984']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 3.241, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 277, 'citation_normalized_percentile': {'value': 0.691574, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 99, 'max': 100}, 'biblio': {'volume': '15', 'issue': '1', 'first_page': '56', 'last_page': '62'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11287', 'display_name': 'Genomic Landscape of Cancer and Mutational Signatures', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1306', 'display_name': 'Cancer Research'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11287', 'display_name': 'Genomic Landscape of Cancer and Mutational Signatures', 'score': 0.9987, 'subfield': {'id': 'https://openalex.org/subfields/1306', 'display_name': 'Cancer Research'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11642', 'display_name': 'Standards and Guidelines for Genetic Variant Interpretation', 'score': 0.9985, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10015', 'display_name': 'RNA Sequencing Data Analysis', 'score': 0.9971, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/genome-annotation', 'display_name': 'genome annotation', 'score': 0.535614}, {'id': 'https://openalex.org/keywords/word-error-rate', 'display_name': 'Word error rate', 'score': 0.52051985}, {'id': 'https://openalex.org/keywords/sequence-variation', 'display_name': 'sequence variation', 'score': 0.51788}], 'concepts': [{'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.68618625}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.63628316}, {'id': 'https://openalex.org/C51679486', 'wikidata': 'https://www.wikidata.org/wiki/Q380546', 'display_name': 'DNA sequencing', 'level': 3, 'score': 0.631415}, {'id': 'https://openalex.org/C64869954', 'wikidata': 'https://www.wikidata.org/wiki/Q1859747', 'display_name': 'False positive paradox', 'level': 2, 'score': 0.5800724}, {'id': 'https://openalex.org/C132917006', 'wikidata': 'https://www.wikidata.org/wiki/Q5250436', 'display_name': 'Deep sequencing', 'level': 4, 'score': 0.5671054}, {'id': 'https://openalex.org/C40969351', 'wikidata': 'https://www.wikidata.org/wiki/Q3516228', 'display_name': 'Word error rate', 'level': 2, 'score': 0.52051985}, {'id': 'https://openalex.org/C141231307', 'wikidata': 'https://www.wikidata.org/wiki/Q7020', 'display_name': 'Genome', 'level': 3, 'score': 0.4218209}, {'id': 'https://openalex.org/C2776459999', 'wikidata': 'https://www.wikidata.org/wiki/Q2119376', 'display_name': 'Fidelity', 'level': 2, 'score': 0.41916007}, {'id': 'https://openalex.org/C197077220', 'wikidata': 'https://www.wikidata.org/wiki/Q720988', 'display_name': 'Human genome', 'level': 4, 'score': 0.41648465}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.39904344}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.28361088}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.17476815}, {'id': 'https://openalex.org/C119857082', 'wikidata': 'https://www.wikidata.org/wiki/Q2539', 'display_name': 'Machine learning', 'level': 1, 'score': 0.16843149}, {'id': 'https://openalex.org/C154945302', 'wikidata': 'https://www.wikidata.org/wiki/Q11660', 'display_name': 'Artificial intelligence', 'level': 1, 'score': 0.15311411}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.1470058}, {'id': 'https://openalex.org/C76155785', 'wikidata': 'https://www.wikidata.org/wiki/Q418', 'display_name': 'Telecommunications', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D015894', 'descriptor_name': 'Genome, Human', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D059014', 'descriptor_name': 'High-Throughput Nucleotide Sequencing', 'qualifier_ui': 'Q000592', 'qualifier_name': 'standards', 'is_major_topic': True}, {'descriptor_ui': 'D059014', 'descriptor_name': 'High-Throughput Nucleotide Sequencing', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D012107', 'descriptor_name': 'Research Design', 'qualifier_ui': 'Q000592', 'qualifier_name': 'standards', 'is_major_topic': True}, {'descriptor_ui': 'D015894', 'descriptor_name': 'Genome, Human', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D059014', 'descriptor_name': 'High-Throughput Nucleotide Sequencing', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012107', 'descriptor_name': 'Research Design', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018401', 'descriptor_name': 'Sample Size', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1038/nrg3655', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S8553189', 'display_name': 'Nature Reviews Genetics', 'issn_l': '1471-0056', 'issn': ['1471-0056', '1471-0064'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319908', 'host_organization_name': 'Nature Portfolio', 'host_organization_lineage': ['https://openalex.org/P4310319908', 'https://openalex.org/P4310319965'], 'host_organization_lineage_names': ['Nature Portfolio', 'Springer Nature'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103745', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/24322726', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103745', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'acceptedVersion', 'is_accepted': True, 'is_published': False}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 101, 'referenced_works': ['https://openalex.org/W1481275998', 'https://openalex.org/W1973950074', 'https://openalex.org/W1978150238', 'https://openalex.org/W1979866423', 'https://openalex.org/W1984708728', 'https://openalex.org/W1985739089', 'https://openalex.org/W1989298969', 'https://openalex.org/W1990601349', 'https://openalex.org/W1995427235', 'https://openalex.org/W1996181407', 'https://openalex.org/W1997478924', 'https://openalex.org/W1998206066', 'https://openalex.org/W2000905326', 'https://openalex.org/W2003342954', 'https://openalex.org/W2003865229', 'https://openalex.org/W2009441527', 'https://openalex.org/W2009720434', 'https://openalex.org/W2012673284', 'https://openalex.org/W2021604692', 'https://openalex.org/W2022316530', 'https://openalex.org/W2026862650', 'https://openalex.org/W2039785774', 'https://openalex.org/W2044394153', 'https://openalex.org/W2046434400', 'https://openalex.org/W2047541315', 'https://openalex.org/W2047612868', 'https://openalex.org/W2049762689', 'https://openalex.org/W2050169817', 'https://openalex.org/W2050886653', 'https://openalex.org/W2051380427', 'https://openalex.org/W2051635612', 'https://openalex.org/W2054185647', 'https://openalex.org/W2054636737', 'https://openalex.org/W2055553707', 'https://openalex.org/W2060987657', 'https://openalex.org/W2062581381', 'https://openalex.org/W2062895902', 'https://openalex.org/W2068917703', 'https://openalex.org/W2071501400', 'https://openalex.org/W2072880634', 'https://openalex.org/W2073428701', 'https://openalex.org/W2073445420', 'https://openalex.org/W2076475433', 'https://openalex.org/W2079859830', 'https://openalex.org/W2087464200', 'https://openalex.org/W2088170018', 'https://openalex.org/W2090247362', 'https://openalex.org/W2092138236', 'https://openalex.org/W2092988184', 'https://openalex.org/W2093343938', 'https://openalex.org/W2094122486', 'https://openalex.org/W2094813938', 'https://openalex.org/W2095267560', 'https://openalex.org/W2095340238', 'https://openalex.org/W2096192437', 'https://openalex.org/W2098131178', 'https://openalex.org/W2099535366', 'https://openalex.org/W2101248947', 'https://openalex.org/W2101250487', 'https://openalex.org/W2101709592', 'https://openalex.org/W2102021831', 'https://openalex.org/W2103154918', 'https://openalex.org/W2103441770', 'https://openalex.org/W2107903949', 'https://openalex.org/W2108487922', 'https://openalex.org/W2111326065', 'https://openalex.org/W2112113834', 'https://openalex.org/W2113312463', 'https://openalex.org/W2118246164', 'https://openalex.org/W2118526609', 'https://openalex.org/W2119180969', 'https://openalex.org/W2119284644', 'https://openalex.org/W2121984048', 'https://openalex.org/W2123062855', 'https://openalex.org/W2124465358', 'https://openalex.org/W2124985265', 'https://openalex.org/W2127173397', 'https://openalex.org/W2129504003', 'https://openalex.org/W2130077037', 'https://openalex.org/W2133920348', 'https://openalex.org/W2136509799', 'https://openalex.org/W2139437267', 'https://openalex.org/W2140159173', 'https://openalex.org/W2143197030', 'https://openalex.org/W2145031157', 'https://openalex.org/W2151372451', 'https://openalex.org/W2153010521', 'https://openalex.org/W2153238155', 'https://openalex.org/W2154397429', 'https://openalex.org/W2159965404', 'https://openalex.org/W2160444236', 'https://openalex.org/W2162370352', 'https://openalex.org/W2164971951', 'https://openalex.org/W2168133698', 'https://openalex.org/W2168142506', 'https://openalex.org/W2168656450', 'https://openalex.org/W2169873962', 'https://openalex.org/W2396325283', 'https://openalex.org/W2989345970', 'https://openalex.org/W4231539689', 'https://openalex.org/W4247053599'], 'related_works': ['https://openalex.org/W4380449851', 'https://openalex.org/W4318832338', 'https://openalex.org/W4248383205', 'https://openalex.org/W3209204065', 'https://openalex.org/W3125091513', 'https://openalex.org/W2381850946', 'https://openalex.org/W2183246718', 'https://openalex.org/W2099261052', 'https://openalex.org/W1919390113', 'https://openalex.org/W1557094818'], 'abstract_inverted_index': {'Next-generation': [0], 'sequencing': [1, 37, 43, 48], 'for': [2], 'variant': [3, 27, 72], 'identification': [4], 'is': [5], 'now': [6], 'becoming': [7], 'widespread,': [8], 'although': [9], 'pipelines': [10], 'have': [11, 40], 'not': [12], 'yet': [13], 'been': [14], 'optimized.': [15], 'In': [16], 'this': [17], 'Perspective': [18], 'article,': [19], 'the': [20, 90], 'authors': [21], 'discuss': [22, 97], 'ways': [23], 'to': [24, 88, 110], 'minimize': [25, 89], 'erroneous': [26], 'calls,': [28], 'in': [29, 35, 59, 71, 102], 'particular,': [30], 'by': [31], 'using': [32], 'replicates.': [33], 'Advances': [34], 'next-generation': [36], '(NGS)': [38], 'technologies': [39], 'rapidly': [41], 'improved': [42], 'fidelity': [44], 'and': [45, 80, 104], 'substantially': [46], 'decreased': [47], 'error': [49, 66], 'rates.': [50], 'However,': [51], 'given': [52], 'that': [53], 'there': [54], 'are': [55], 'billions': [56], 'of': [57, 92, 99], 'nucleotides': [58], 'a': [60], 'human': [61], 'genome,': [62], 'even': [63], 'low': [64], 'experimental': [65, 100], 'rates': [67], 'yield': [68], 'many': [69], 'errors': [70, 101], 'calls.': [73], 'Erroneous': [74], 'variants': [75], 'can': [76, 107], 'mimic': [77], 'true': [78], 'somatic': [79], 'rare': [81], 'variants,': [82], 'thus': [83], 'requiring': [84], 'costly': [85], 'confirmatory': [86], 'experiments': [87], 'number': [91], 'false': [93], 'positives.': [94], 'Here,': [95], 'we': [96], 'sources': [98], 'NGS': [103], 'how': [105], 'replicates': [106], 'be': [108], 'used': [109], 'abate': [111], 'such': [112], 'errors.': [113]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2009783955', 'counts_by_year': [{'year': 2024, 'cited_by_count': 17}, {'year': 2023, 'cited_by_count': 19}, {'year': 2022, 'cited_by_count': 22}, {'year': 2021, 'cited_by_count': 17}, {'year': 2020, 'cited_by_count': 32}, {'year': 2019, 'cited_by_count': 25}, {'year': 2018, 'cited_by_count': 22}, {'year': 2017, 'cited_by_count': 28}, {'year': 2016, 'cited_by_count': 44}, {'year': 2015, 'cited_by_count': 30}, {'year': 2014, 'cited_by_count': 20}], 'updated_date': '2024-09-20T00:46:54.833902', 'created_date': '2016-06-24'}