Title: Kinetic evidence for the presence of two forms of M2-type pyruvate kinase in rat small intestine
Abstract: Some kinetic properties of pyruvate kinase from rat small intestine have been investigated. The relative insensitivity of the enzyme to ATP inhibition and the amino acid inhibition pattern allows the conclusion that intestinal pyruvate kinase belongs to the M2-type. The pyruvate kinase activity as a function of the phosphoenol pyruvate concentration is characterized by two different n values. The activity correlating with the low n value is stimulated by Fru-1,6-P2, whereas the activity at higher phosphoenol pyruvate concentrations is not influenced by this glycolytic intermediate. These results, together with the partial relief of the amino acid inhibition by Fru-1,6-P2, show that two forms of the enzyme are present with different kinetic properties. The metabolic implication of the kinetic properties of pyruvate kinase for rat small intestine is discussed.
Publication Year: 1974
Publication Date: 1974-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 22
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