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1108, 1116, 1127, 1130, 1173, 1178, 1236, 1240, 1331, 1349, 1371, 1430, 1466, 1477, 1551, 1558, 1605, 1629], 'single': [44, 199, 317, 810, 825, 1467, 1482], 'chain': [45, 200, 318, 811, 826, 1468, 1483], 'antibody': [46, 201, 322, 827, 1484], 'raised': [47, 202, 814, 1471], 'against': [48, 203, 815, 1472], 'lipopolysaccharide': [49, 97, 204, 252, 316, 817, 1474], 'outer': [52, 207], 'wall': [54, 209], 'Gram-negative': [56, 87, 211, 242, 821, 1478], 'bacteria': [57, 88, 212, 243], '(abbreviated': [58, 213], 'scFv-GFP).': [60, 215], 'The': [61, 99, 216, 254, 325, 484, 513, 561, 952, 981, 1140, 1169, 1217, 1610], 'scFv': [62, 217], 'moiety': [63, 218, 794, 1451], 'was': [64, 67, 219, 222, 828, 871, 915, 950, 958, 1485, 1529, 1573, 1608, 1616], 'functional': [65, 220], 'proven': [68, 223], 'binding': [70, 83, 225, 238], 'assays,': [71, 226], 'which': [72, 227], 'involved': [73, 228], 'use': [75, 230], 'both': [77, 232, 860, 1518], 'fluorescence': [78, 109, 233, 264, 313, 919, 1577], 'correlation': [79, 114, 234, 269, 314, 868, 1526], 'spectroscopy': [80, 235, 315, 869, 1527], 'observing': [81, 236], 'scFv-GFP': [85, 103, 117, 151, 240, 258, 272, 306, 890, 914, 1548, 1572], 'surface': [91, 246], 'plasmon': [92, 247], 'resonance': [93, 248], 'containing': [95, 250], 'adsorbed': [96, 251], 'antigen.': [98, 253], 'rotational': [100, 113, 255, 268, 907, 1565], 'motion': [101, 141, 256, 296, 908, 976, 1566, 1634], 'has': [104, 259, 334, 467, 491, 545, 569, 990, 1123, 1147, 1201, 1225], 'been': [105, 260, 492, 546, 570, 681, 1148, 1202, 1226, 1338], 'time-resolved': [108, 263, 918, 1576], 'anisotropy.': [110, 265, 920, 1578], 'However,': [111, 266], 'time': [115, 270], 'too': [119, 274], 'short': [120, 275], 'account': [122, 277], 'for': [123, 278, 343, 847, 940, 999, 1505, 1598, 1653, 1664], 'globular': [124, 279], 'rotation': [125, 280, 944, 1602], 'whole': [128, 283], 'protein.': [129, 284, 731, 1388], 'This': [130, 285], 'result': [131, 286], 'can': [132, 287, 737, 1394], 'only': [133, 288], 'be': [134, 289, 577, 738, 1233, 1395], 'explained': [135, 290], 'by': [136, 291, 521, 970, 1177, 1628], 'assuming': [137, 292], 'fast': [139, 294], 'hinge': [140, 295, 973, 1631], 'between': [142, 297], 'two': [144, 299, 963, 1621], 'proteins.': [146, 301], 'A': [147, 302, 540, 1196], 'modeled': [148, 303], 'structure': [149, 304, 486, 563, 957, 1142, 1219, 1615], 'supports': [152, 307], 'this': [153, 308], 'observation.': [154, 309], 'fragment': [319, 813, 1470], 'variable': [323], 'domains': [324], '(GFP)1': [329, 985], 'from': [330, 967, 986, 1625, 1645, 1661], 'jellyfishAequorea': [332, 988], 'received': [335, 991], 'widespread': [336, 992], 'utilization': [337, 993], 'natural': [340, 996], 'marker': [342, 998], 'gene': [344, 348, 687, 1000, 1004, 1344], 'expression,': [345, 1001], 'localization': [346, 1002], 'products': [349, 1005], '(1.Heim': [350, 1006], 'R.': [351, 391, 406, 1007, 1047, 1062], 'Prasher': [352, 379, 496, 1008, 1035, 1152], 'D.C.': [353, 433, 497, 1009, 1089, 1153], 'Tsien': [354, 394, 407, 595, 632, 1010, 1050, 1063, 1251, 1288], 'R.Y.': [355, 395, 408, 549, 596, 633, 1011, 1051, 1064, 1205, 1252, 1289], 'Proc.': [356, 638, 1012, 1294], 'Natl.': [357, 639, 1013, 1295], 'Acad.': [358, 640, 1014, 1296], 'Sci.': [359, 641, 1015, 1297], 'U.': [360, 642, 1016, 1298], 'S.': [361, 425, 643, 1017, 1081, 1299], 'A.': [362, 393, 644, 760, 1018, 1049, 1300, 1417], '1994;': [363, 382, 1019, 1038], '91:': [364, 1020], '12501-12504Crossref': [365, 1021], 'PubMed': [366, 385, 400, 419, 438, 508, 556, 603, 619, 648, 667, 766, 1022, 1041, 1056, 1075, 1094, 1164, 1212, 1259, 1275, 1304, 1323, 1423], 'Scopus': [367, 386, 401, 420, 439, 509, 557, 604, 620, 649, 668, 767, 1023, 1042, 1057, 1076, 1095, 1165, 1213, 1260, 1276, 1305, 1324, 1424], '(1476)': [368, 1024], 'Google': [369, 388, 403, 422, 441, 511, 559, 606, 622, 651, 670, 769, 1025, 1044, 1059, 1078, 1097, 1167, 1215, 1262, 1278, 1307, 1326, 1426], 'Scholar,': [370, 389, 404, 423, 607, 623, 652, 1026, 1045, 1060, 1079, 1263, 1279, 1308], '2.Chalfie': [371, 1027], 'M.': [372, 588, 635, 1028, 1244, 1291], 'Tu': [373, 1029], 'Y.': [374, 1030], 'Euskirchen': [375, 1031], 'G.': [376, 1032], 'Ward': [377, 502, 1033, 1158], 'W.': [378, 1034], 'D.': [380, 1036], 'Science.': [381, 599, 1037, 1255], '263:': [383, 1039], '802-805Crossref': [384, 1040], '(5451)': [387, 1043], '3.Heim': [390, 1046], 'Cubitt': [392, 589, 1048, 1245], 'Nature.': [396, 1052], '1995;': [397, 435, 1053, 1091], '373:': [398, 1054], '663-664Crossref': [399, 1055], '(1508)': [402, 1058], '4.Heim': [405, 1061], 'Curr.': [409, 1065], 'Biol.': [410, 1066], '1996;': [411, 600, 616, 1067, 1256, 1272], '6:': [412, 1068], '178-182Abstract': [413, 1069], 'Full': [414, 416, 1070, 1072], 'Text': [415, 417, 1071, 1073], 'PDF': [418, 1074], '(1191)': [421, 1077], '5.Delagrave': [424, 1080], 'Hawtin': [426, 1082], 'R.E.': [427, 1083], 'Silva': [428, 1084], 'C.M.': [429, 1085], 'Yang': [430, 1086], 'M.M.': [431, 1087], 'Youvan': [432, 1088], 'Bio/Technology.': [434, 1090], '13:': [436, 1092], '151-154Crossref': [437, 1093], '(265)': [440, 1096], 'Scholar),': [442, 1098], 'identification': [444, 1100], 'interaction': [447, 887, 1103, 1545], 'function.': [449, 690, 1105, 1347], 'consisting': [454, 1110], '238': [456, 1112], 'amino': [457, 744, 751, 1113, 1401, 1408], 'acids': [458, 745, 752, 1114, 1402, 1409], 'molecular': [461, 1117], 'mass': [462, 1118], '27': [464, 1120], 'kDa': [465, 1121], 'shape': [469, 1125], 'cylinder': [472, 1128], 'length': [475, 1131], '4.2': [477, 1133], 'nm': [478, 1134], 'diameter': [480, 1136], '2.4': [482, 1138], 'nm.': [483, 1139], 'chemical': [485, 1141], 'hexapeptide': [489, 575, 1145, 1231], 'chromophore': [490, 1146], 'elucidated': [493, 1149], '(6.Cody': [494, 1150], 'C.W.': [495, 1151], 'Westler': [498, 1154], 'W.M.': [499, 1155], 'Prendergast': [500, 1156], 'F.G.': [501, 1157], 'W.W': [503, 1159], 'Biochemistry.': [504, 663, 1160, 1319], '1993;': [505, 1161], '32:': [506, 1162], '1212-1218Crossref': [507, 1163], '(571)': [510, 1166], 'Scholar).': [512, 560, 770, 1168, 1216, 1327, 1427], 'intrinsic': [514, 1170], 'fluorophore': [515, 1171], 'p-hydroxybenzylidene-imidazolidine': [518, 1174], 'derivative': [519, 1175], 'formed': [520, 1176], 'covalent': [523, 1179], 'modification': [524, 1180], 'sequence': [527, 1183], 'Ser65': [528, 1184], '(or': [529, 1185], 'Thr65': [530, 1186], 'enhanced': [532, 567, 806, 1188, 1223, 1463], 'GFP),': [533, 1189], 'Tyr66,': [534, 1190], 'Gly67': [536, 1192], 'hexapeptide.': [539, 1195], 'comprehensive': [541, 1197], 'review': [542, 1198], 'published': [547, 1203], '(7.Tsien': [548, 1204], 'Annu.': [550, 1206], 'Rev.': [551, 1207], 'Biochem.': [552, 1208], '1998;': [553, 1209], '67:': [554, 1210], '509-544Crossref': [555, 1211], '(4882)': [558, 1214], 'crystal': [562, 1218], 'solved': [571, 1227], 'showed': [573, 1229], 'part': [578, 912, 1234, 1570], 'central': [581, 1237], 'helix': [582, 1238], 'inside': [583, 1239], '11-stranded': [585, 1241], 'β-barrel': [586, 1242], '(8.Ormö': [587, 1243], 'A.B.': [590, 1246], 'Kallio': [591, 657, 1247, 1313], 'K.': [592, 625, 658, 1248, 1281, 1314], 'Gross': [593, 1249], 'L.A.': [594, 1250], 'Remington': [597, 636, 661, 1253, 1292, 1317], 'S.J.': [598, 637, 662, 1254, 1293, 1318], '273:': [601, 1257], '1392-1395Crossref': [602, 1258], '(1902)': [605, 1261], '9.Yang': [608, 1264], 'F.': [609, 1265], 'Moss': [610, 1266], 'L.G.': [611, 1267], 'Phillips': [612, 1268], 'G.N.': [613, 1269], 'Nat.': [614, 1270], 'Biotechnol.': [615, 1271], '14:': [617, 1273], '1246-1251Crossref': [618, 1274], '(1286)': [621, 1277], '10.Brejc': [624, 1280], 'Sixma': [626, 1282], 'T.K.': [627, 1283], 'Kitts': [628, 1284], 'P.A.': [629, 1285], 'Kain': [630, 1286], 'S.R.': [631, 1287], 'Ormö': [634, 1290], '1997;': [645, 1301], '94:': [646, 1302], '2306-2311Crossref': [647, 1303], '(647)': [650, 1306], '11.Elsiger': [653, 1309], 'M.A.': [654, 1310], 'Wachter': [655, 1311], 'R.M.': [656, 1312], 'Hanson': [659, 1315], 'G.T.': [660, 1316], '1999;': [664, 763, 1320, 1420], '38:': [665, 1321], '5296-5301Crossref': [666, 1322], '(288)': [669, 1325], 'Scholar).Genetic': [671], 'variety': [675, 1332], 'numerous': [684, 1341], 'studies': [685, 1342], 'or': [688, 724, 1345, 1381], 'In': [691, 705, 905, 1348, 1362, 1563], 'sense': [693, 1350], 'it': [694, 1351], 'miraculous': [696, 1353], 'that': [697, 753, 961, 1354, 1410, 1619], 'most': [699, 1356], 'fusion': [700, 708, 730, 784, 796, 837, 852, 880, 891, 934, 1357, 1365, 1387, 1441, 1453, 1494, 1510, 1538, 1549, 1592], 'functional.': [704, 1361], 'many': [706, 1363], 'other': [707, 969, 1364, 1627], 'proteins,': [709, 785, 1366, 1442], 'reporter': [715, 1372], 'does': [716, 1373], 'often': [717, 1374], 'not': [718, 780, 1375, 1437], 'fold': [719, 1376], 'well,': [720, 1377], 'resulting': [721, 1378], 'aggregates': [723, 1380], 'inclusion': [725, 1382], 'bodies': [726, 1383], 'entire': [729, 1386], 'Sometimes': [732, 1389], 'causes': [734, 1391], 'aggregation': [736, 1393], 'attributed': [739, 1396], 'certain': [741, 1398], '(clusters': [742, 1399], 'of)': [743, 1400], 'such': [746, 1403], 'hydrophobic': [748, 1405], 'clusters': [749, 1406], 'become': [754, 1411], 'solvent': [755, 1412], 'exposed': [756, 1413], '(12.Wall': [757, 1414], 'J.G.': [758, 1415], 'Plückthun': [759, 1416], 'Prot.': [761, 1418], 'Eng.': [762, 1419], '12:': [764, 1421], '605-611Crossref': [765, 1422], '(33)': [768, 1425], 'To': [771, 936, 1428, 1594], 'obtain': [772, 937, 1429, 1595], 'better': [774, 1431], 'picture': [775, 1432], 'why': [776, 1433], 'these': [777, 1434], 'phenomena': [778, 1435], 'do': [779, 1436], 'occur': [781, 1438], 'we': [786, 804, 843, 1443, 1461, 1501], 'behavior': [790, 1447], 'emphasized': [799, 1456], 'motional': [801, 929, 1458, 1587], 'properties.': [802, 1459], 'Thereto,': [803, 1460], 'Fv': [812, 1469], '(LPS)': [818, 1475], 'bacterium.': [822, 1479], 'Because': [823, 1480], 'linked': [829, 1486], 'N-terminal': [832, 1489], 'residue': [833, 1490], 'GFP,': [835, 1492], 'abbreviated': [840, 1497], 'scFv-GFP.Here': [842], 'report': [844, 1502], 'relevant': [846, 1504], 'dynamics': [849, 930, 1507, 1588], 'monitor': [856, 1514], 'function': [858, 1516], 'vitroand': [862, 1520], 'vivo': [864, 1522], 'biological': [865, 1523], 'systems.': [866, 1524], 'Fluorescence': [867, 1525], '(FCS)': [870, 1528], 'measure': [874, 1532], 'translational': [876, 1534], 'diffusion': [877, 1535], 'visualize': [885, 1543], 'much': [894, 901, 1552, 1559], 'larger': [895, 1553], 'ligand': [896, 1554], '(i.e.Gram-negative': [897, 1555], 'bacteria),': [898, 1556], 'having': [899, 1557], 'slower': [902, 1560], 'translation': [903, 1561], 'diffusion.': [904, 1562], 'addition,': [906, 1564], 'studied': [916, 1574], 'Both': [921, 1579], 'techniques': [922, 1580], 'were': [923, 1581], 'then': [924, 1582], 'characterize': [927, 1585], 'construct.': [935, 1593], 'additional': [938, 1596], 'support': [939, 1597], 'observed': [942, 1600], 'rapid': [943, 1601], 'scFv-GFP,': [946, 1604], 'structural': [948, 1606], 'model': [949, 1607], 'built.': [951, 1609], 'incentive': [953, 1611], 'present': [955, 1613], 'demonstrate': [960, 1618], 'separated': [966, 1624], 'each': [968, 1626], 'flexible': [972, 1630], 'allowing': [974, 1632], 'free': [975, 1633], 'no': [978, 1636], 'mutual': [979, 1637], 'interference.': [980, 1638], 'Genetic': [1328], 'scFv-GFP.': [1499], 'Here': [1500], 'thank': [1640], 'Jan': [1641], 'van': [1642], 'der': [1643], 'Wolf': [1644], 'Instituut': [1646], 'voor': [1647], 'Plantenziektenkundig': [1648], 'Onderzoek-Dienst': [1649], 'Landouwkundig': [1650], 'Onderzoek': [1651], '(IPO-DLO)': [1652], 'supplying': [1654], 'bacterial': [1656], 'cultures': [1657], 'Maurice': [1659], 'Kunen': [1660], 'Maastricht': [1662], 'University': [1663], 'assistance': [1665], 'BIAcore': [1668], 'experiments.': [1669]}, 'cited_by_api_url': 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