Title: Porcine Sugar Nucleotide: Glycoprotein Glycosyltransferases. III. Blood Serum and Liver <i>N</i>-Acetylglucosaminyltransferase
Abstract: Pork liver and serum contain UDP-N-acetylglucosamine: glycoprotein N-acetylglucosaminyltransferase activity. The serum enzyme is soluble and the liver enzyme is membrane-bound; the latter can be solubilized by organic solvent extraction but the yield is low and the soluble preparation is unstable. Comparison of membrane-bound and soluble liver enzymes indicates that the membrane has only a minor modifying effect on the enzyme kinetics. Comparison of serum and liver enzymes shows that the two activities are similar but not identical; in particular, the two enzymes have similar cation requirements, substrate specificities, pH profiles, and K m values for both nucleotide sugar and glycoprotein acceptor. The source and function of the serum enzyme cannot be deduced from this information.
Publication Year: 1971
Publication Date: 1971-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 22
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