Title: Modified Technique for the Development of Paper Chromatograms
Abstract: IN the original publication by Consden, Gordon and Martin1 on the application of partition chromatography on filter paper to the separation of amino-acids, the positions of these acids were determined by spraying the whole of the paper with a 0 1 or 0·05 per cent solution of ninhydrin in butanol. This procedure appears to have been adopted by subsequent workers in spite of inherent objections to the use of butanol in a fine spray in more or less close proximity to the operator. To avoid this objection, and to determine whether this stage could be entirely eliminated, a series of 'runs' has been made in which the ninhydrin has been incorporated in the moving phase.