Title: Differential expression of the transfected liver-specific α1-inhibitor III gene in normal hepatocytes and hepatoma cells in culture
Abstract: Normal and malignant hepatocytes were transfected during log phase culture with a nested series of DNA plasmids containing 5′-flanking regions of the rat liver-specific acute phase plasma proteinase α1-inhibitor III (α1I3) gene. Under these conditions, luciferase reporter gene expression in primary adult rat and mouse hepatocytes was 10-fold higher than luciferase expression in hepatoma lines (human HepG2 and Hep3B; rat FAZA). Optimal expression in primary rat hepatocytes required regions stretching 2214 bp 5′-upstream of the transcription start site. Shorter 5′-flanking sequences were optimal for expression in hepatoma cells(−1025 and −186 bp for rat and human lines, respectively) and primary mouse hepatocytes (−225 bp). In contrast, regions from −186 to −225 bp drove luciferase expression in primary rat hepatocytes, but only 20–75% of optimal levels. Qualitative differences were unaccounted for by non-equivalent uptake of plasmid DNA, suggesting that tissue specific gene expression is regulated differently in normal and malignant cells, and with apparent species specificity.
Publication Year: 1992
Publication Date: 1992-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 3
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