Title: Isolation of Chlorella vulgaris and its DNA extraction methods
Abstract: [Objective] The aim of this study is to isolate Chlorella vulgaris (chlorella) and extract its genomic DNA. [Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples; the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method. [Result] The proper conditions for chlorella culture were as following: temperature 20-25℃, illumination 4.39-5.86 W/m2 and rotational speed 100-150 r/min; improved CTAB method was suitable for extracting genomic DNA from chlorella. [Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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