Title: Use of Random Amplified Polymorphic DNA (RAPD) to Study Genetic Diversity among Romanian Local Vine (Vitis vinifera L.) Cultivars
Abstract: To estimate genetic relationships among 36 local vine cultivars, RAPD analysis was performed with 24 decamer primers selected from a total of 40 primers. These primers generated polymorphic bands among the studied genotypes. The purity and amount (ng/?L) of total DNA extracted from each sample were sufficient for optimal RAPD analysis. UPGMA dendrogram was constructed based on genetic distances using the program Tree View. The genotypes analyzed clustered into three main groups and the values of genetic distances between data shows that there are differences at molecular level.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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