Abstract: Chapter 4 Single-Use Bioreactors—An Overview Regine Eibl, Regine EiblSearch for more papers by this authorChristian Löffelholz, Christian LöffelholzSearch for more papers by this authorDieter Eibl, Dieter EiblSearch for more papers by this author Regine Eibl, Regine EiblSearch for more papers by this authorChristian Löffelholz, Christian LöffelholzSearch for more papers by this authorDieter Eibl, Dieter EiblSearch for more papers by this author Book Editor(s):Regine Eibl, Regine EiblSearch for more papers by this authorDieter Eibl, Dieter EiblSearch for more papers by this author First published: 08 December 2010 https://doi.org/10.1002/9780470909997.ch4Citations: 12 AboutPDFPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShareShare a linkShare onEmailFacebookTwitterLinkedInRedditWechat Summary This chapter contains sections titled: Introduction Single-Use Bioreactor History Comparison of the Current, Most Common Single-Use Bioreactors Decision Criteria for Selection of the Most Suitable Single-Use Bioreactor Single-Use Bioreactors—Summary and Future Trends References Fenge C, Lüllau E. (2006). Cell culture bioreactors. In SS Ozturk, WS Hu (eds.), Cell Culture Technology for Pharmaceutical and Cell-Based Therapies. New York: CRC Press, pp. 155–224. Google Scholar Marks DM. (2003). Equipment design considerations for large scale cell culture. Cytotechnology 42: 21–33. 10.1023/A:1026103405618 CASPubMedWeb of Science®Google Scholar Henzler HJ. (2000). Particle stress in bioreactors. In K Schügerl, G Kretzmer (eds.), Influence of Stress on Cell Growth and Product Formation, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 67. Berlin; Heidelberg: Springer, pp. 38–82. Google Scholar Nienow AW. (2006). Reactor engineering in large scale animal cell culture. Cytotechnolgy 50: 9–33. 10.1007/s10616-006-9005-8 CASPubMedWeb of Science®Google Scholar Eibl R, Eibl D. (2007). Disposable bioreactors for cell culture-based bioprocessing. ACHEMA Worldwide News 2: 8–10. Google Scholar Eibl R, Eibl D. (2007). Disposable bioreactors for inoculum production and protein expression. In R Pörtner (ed.), Animal Cell Biotechnology: Methods and Protocols, Series: Methods in Biotechnology, Vol. 24. Totowa, NJ: Humana Press, pp. 321–335. 10.1007/978-1-59745-399-8_15 Google Scholar Eibl R, Eibl D. (2009). Application of disposable bag bioreactors in tissue engineering and for the production of therapeutic proteins. In C Kasper, M van Griensven, R Pörtner (eds.), Bioreactor Systems for Tissue Engineering, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 112. Berlin; Heidelberg: Springer, pp. 183–207. Web of Science®Google Scholar Wilson JS. (2006). A fully disposable monoclonal antibody manufacturing train. BioProcess Int. 4 (Suppl. 4): 34–36. Google Scholar Genzel Y, Reichl U. (2007). Vaccine production: State of the art and future needs in upstream processing. In R Pörtner (ed.), Animal Cell Biotechnology: Methods and Protocols, Series: Methods in Biotechnology, Vol. 24. Totowa, NJ: Humana Press, pp. 457–473. 10.1007/978-1-59745-399-8_21 Google Scholar Scott C. (2008). Biotech leads a revolution in vaccine manufacturing. BioProcess Int. 6 (Suppl. 6): 12–18. Google Scholar Hamis LS, Green C, Leshinsky N, Markham E, Miller K, Craig S. (2004). GMP production and testing of Xcellerated T cells for the treatment of patients with CLL Cytotherapy 6: 554–562. 10.1080/14653240410005348 CASPubMedWeb of Science®Google Scholar GE Healthcare. (2008). Rapid production of clinical grade T lymphocytes in the Wave Bioreactor. Available: http://www.5.gelifesciences.com/aptrix/upp0091.nsf./Content/F7AD616DACC22171C125747400812B51/$file/28933149AA.pdf. Accessed August 15,2009. Google Scholar Negrete A, Kotin RM. (2008). Large-scale production of recombinant adeno-associated viral vectors. In JM Le Doux (ed.), Gene Therapy Protocols: Vol. I, Series: Methods in Molecular Biology, Vol. 433. Totowa, NJ: Humana Press, pp. 79–96. 10.1007/978-1-59745-237-3_5 Google Scholar Hsiao TY, Bacani FT, Carvalho EB, Curtis WR. (1999). Development of a low capital investment reactor system: Application for plant cell suspension culture. Biotechnol. Prog. 15: 114–122. 10.1021/bp980103+ CASPubMedWeb of Science®Google Scholar Palazón J, Mallol A, Eibl R, Lettenbauer C, Cusidó RM, Piñol MT. (2003). Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Med. 69: 344–349. 10.1055/s-2003-38873 CASPubMedWeb of Science®Google Scholar Bentebibel S, Moyano E, Palazón J, Cusidó RM, Bonfill M, Eibl R, Piñol MT. (2005). Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnol. Bioeng. 89: 647–655. 10.1002/bit.20321 CASPubMedWeb of Science®Google Scholar Georgiev MI, Weber J, Maciuk A. (2009). Bioprocessing of plant cell cultures for mass propagation of targeted compounds. Appl. Microbiol. Biotechnol. 83: 809–823. 10.1007/s00253-009-2049-x CASPubMedWeb of Science®Google Scholar Eibl R, Werner S, Eibl D. (2009). Disposable bioreactors for plant liquid cultures at litre-scale. Eng. Life Sci. 9: 156–164. 10.1002/elsc.200800102 CASWeb of Science®Google Scholar Eibl R, Werner S, Eibl D. (2009). Bag bioreactor based on wave-induced motion: Characteristics and applications. In D Eibl, R Eibl (eds.), Disposable Bioreactors, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 115. Berlin; Heidelberg: Springer, pp. 55–87. 10.1007/10_2008_15 Web of Science®Google Scholar Mikola M, Seto J, Amanullah A. (2007). Evaluation of a novel Wave Bioreactor cellbag for aerobic yeast cultivation. Bioprocess Biosyst. Eng. 30: 231–241. 10.1007/s00449-007-0119-y CASPubMedWeb of Science®Google Scholar Eibl R, Eibl D. (2009). Disposable bioreactors in cell culture-based upstream processing. BioProcess Int. 7 (Suppl. 1): 18–23. CASGoogle Scholar Falch FA, Heden CG. (1963). Disposable shaker flasks. Biotechnol. Bioeng. 5: 211–220. 10.1002/bit.260050306 CASWeb of Science®Google Scholar Knazek RA, Gullino PM, Kohler PO, Dedrick RL. (1972). Ceil culture on artificial capillaries: An approach to tissue growth in vitro. Science 178: 65–67. 10.1126/science.178.4056.65 CASPubMedWeb of Science®Google Scholar Hopkinson J. (1985). Hollow fibre cell culture systems for economical cell-product manufacturing. BioTechnology 3: 225–230. 10.1038/nbt0385-225 Web of Science®Google Scholar Gorter A, van de Griend RJ, van Eendenburg JD, Haasnot WH, Fleuren GJ. (1993). Production of bi-specific monoclonal antibodies in a hollow-fibre bioreactor. J. Immunol. Methods 161: 145–150. 10.1016/0022-1759(93)90289-J CASPubMedWeb of Science®Google Scholar Marx U. (1998). Membrane-based cell culture technologies: A scientifically economically satisfactory alternative malignant ascites production for monoclonal antibodies. Res. Immunol. 6: 557–559. 10.1016/S0923-2494(98)80006-0 Web of Science®Google Scholar Brecht R. (2009). Disposable bioreactors—Maturation into pharmaceutical glycoprotein manufacturing. In D Eibl, R Eibl (eds.), Disposable Bioreactors, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 115. Berlin; Heidelberg: Springer, pp. 1–31. 10.1007/10_2008_33 Web of Science®Google Scholar Davis JM. (2007). Hollow fibre cell culture. In R Pörtner (ed.), Animal Cell Biotechnology: Methods and Protocols, Series Methods in Biotechnology, Vol. 24. Totowa, NJ: Humana Press, pp. 337–352. 10.1007/978-1-59745-399-8_16 Google Scholar Norris BJ, Gramer MJ, Hirschek MD. (2000). Growth of cell lines in bioreactors. In GC Howard, DR Bethell (eds.), Basic Methods in Antibody Production and Characterization. Boca Raton, FL: CRC Press, pp. 87–104. 10.1201/9781420036534.ch9 Google Scholar Davis JM. (2007). Systems for cell culture scale-up. In G Stacey, JM Davis (eds.), Medicines from Animal Cell Culture. Chichester, UK: John Wiley & Sons, pp. 145–171. 10.1002/9780470723791.ch10 Google Scholar Schwander E, Rasmusen H. (2005). Scalable, controlled growth of adherent cells in a disposable, multilayer format. Genet Eng. Biotechnol. News 25: 29. Google Scholar DePalma A. (2002). Options for anchorage-dependent cell culture. Genet Eng. Biotechnol. News 22: 58–62. Google Scholar Bishop NE, Hugo DL, Borovec SV, Anderson DA. (1994). Rapid and efficient purification of hepatitis A virus from cell culture. J. Virol. Methods 47: 203–216. 10.1016/0166-0934(94)90078-7 CASPubMedWeb of Science®Google Scholar Nelson K, Bielicki J, Anson DS. (1997). Immobilization and characterization of a cell line exhibiting a severe multiple sulphatase deficiency phenotype. Biochem. J. 326: 125–130. 10.1042/bj3260125 CASPubMedWeb of Science®Google Scholar Lee SY, Kim SH, Kim VN, Hwang JH, Jin M, Lee J, Kim S. (1999). Heterologous gene expression in avian cells: Potential as a producer of recombinant proteins. J. Biomed. Sci. 6: 8–17. 10.1007/BF02256418 CASPubMedWeb of Science®Google Scholar Hagen AJ, Aboud RA, DePhillips PA, Oliver CN, Orella CJ, Sitrin RD. (1996). Use of nuclease enzyme in the purification of VAQTA, a hepatits A vaccine. Biotechnol. Appl. Biochem. 23: 209–215. CASPubMedWeb of Science®Google Scholar Bail P, Crawford B, Lindström K. (2009). 21st century vaccine manufacturing. BioProcess Int. 4: 18–28. Google Scholar Beeksma LA, Kompier R. (1995). Cell growth and virus propagation in the Costar Cell Cube system. In EC Beuvery, JB Griffiths, WP Zeijlemaker (eds.), Animal Cell Technology: Developments towards the 21st Century. Dordrecht, The Netherlands: Kluwer, pp. 661–663. 10.1007/978-94-011-0437-1_103 Web of Science®Google Scholar Aunins JB, Bibila TA, Gatchalian S, Hunt GR, Junker BH, Lewis JA, Seifert DB, Licari P, Ramasubramanyan K, Ranucci CS, Seamans TC, Zhou W, Waterbury W, Buckland BC. (1997). Reactor development for the hepatitis A vaccine VAQTA. In MJT Carrondo, B Griffiths, JLP Moreira (eds.), Animal Cell Technology: From Vaccine to Genetic Medicine. Dordrecht, The Netherlands: Kluwer, pp. 175–183. 10.1007/978-94-011-5404-8_29 Web of Science®Google Scholar Ziv M, Ronen G, Raviv M. (1998). Proliferation of meristematic clusters in disposable pre-sterilized plastic biocontainers for the large-scale propagation of plants. In Vitro Cell Dev. Biol. Plant 34: 152–158. 10.1007/BF02822781 Web of Science®Google Scholar Ziv M. (1999). Organogenic plant regeneration in bioreactors. In A Altmann, M Ziv, S Izhar (eds.), Plant Biotechnology and In Vitro Biology in the 21st Century. Dordrecht, The Netherlands: Kluwer, pp. 673–676. 10.1007/978-94-011-4661-6_152 Web of Science®Google Scholar Ziv M. (2000). Bioreactor technology for plant micropropagation. Hort. Rev. 24: 1–30. CASGoogle Scholar Ziv M. (2005). Simple bioreactors for mass propagation of plants. Plant Cell Tissue Organ Cult 81: 277–285. 10.1007/s11240-004-6649-y Web of Science®Google Scholar Curtis WR. (1999). Achieving economic feasibility for moderate-value food and flavour additives. In T Fu, G Singh, WR Curtis (eds.), Plant Cell and Tissue Culture for the Production of Food Ingredients. New York: Kluwer Academic, pp. 225–236. 10.1007/978-1-4615-4753-2_19 Google Scholar Curtis WR. (2004). Growing cells in a reservoir formed of a flexible sterile plastic liner. United States Patent 6709862B2. Google Scholar Falkenberg FW. (1998). Production of monoclonal antibodies in the miniPerm bioreactor: Comparison with other hybridoma culture methods. Res. Immunol. 6: 560–570. 10.1016/S0923-2494(98)80007-2 Google Scholar McArdle J. (2004). Report of the workshop on monoclonal antibodies. ATM 32 (Suppl. 1): 119–122. CASGoogle Scholar Trebak M, Chong JM, Herlyn D, Speicher DW. (1999). Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology. J. Immunol. Methods 230: 59–70. 10.1016/S0022-1759(99)00122-2 CASPubMedWeb of Science®Google Scholar Bruce MP, Boyd V, Duch C, White JR. (2002). Dialysis-based bioreactor systems for the production of monoclonal antibodies—Alternatives to ascites production in mice. J. Immunol. Methods 264: 59–68. 10.1016/S0022-1759(02)00081-9 CASPubMedWeb of Science®Google Scholar Adam E, Sarrazin S, Landolfi C, Motte V, Lortat-Jacob H, Vassalle P, Delehedde M. (2008). Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor. Biochem. Biophys. Res. Commun. 369: 297–302. 10.1016/j.bbrc.2008.01.141 CASPubMedWeb of Science®Google Scholar Jain E, Kumar A. (2008). Upstream processes in antibody production: Evaluation of critical parameters. Biotechnol. Adv. 26: 46–72. 10.1016/j.biotechadv.2007.09.004 CASPubMedWeb of Science®Google Scholar McDonald KA, Hong LM, Trombly DM, Xie Q, Jackman AP. (2005). Production of human α-l-antitrypsin from transgenic rice cell culture in a membrane bioreactor. Biotechnol. Prog. 21: 728–734. 10.1021/bp0496676 CASPubMedWeb of Science®Google Scholar Mitchell JP, Court J, Mason MD, Tabi Z, Clayton A. (2008). Increased exosome production from tumor cell cultures using the Integra CELLine culture system. J. Immunol, Methods 335: 98–105. 10.1016/j.jim.2008.03.001 CASPubMedWeb of Science®Google Scholar Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokihisa T, Iwamoto I, Nakajima H, (2006). IL-25 enhances allergic airway inflammation by amplifying a TH2-cell dependent pathway in mice. J. Allergy Clin. Immunol. 118: 606–614. 10.1016/j.jaci.2006.04.051 CASPubMedWeb of Science®Google Scholar Willet BJ, McMonagle EL, Logan N, Samman A, Hosie MJ. (2008). A single site for N-linked glycosylation in the envelope glycoprotein of feline immunodeficiency virus modulates the virus-receptor interaction. Retrovirology 5: 77–93. 10.1186/1742-4690-5-77 CASPubMedWeb of Science®Google Scholar Kybal J, Vlcek V. (1976). A simple device for stationary cultivation of microorganisms. Biotechnol. Bioeng. 18: 1713–1718. 10.1002/bit.260181206 CASPubMedWeb of Science®Google Scholar Kybal J, Sikyta B. (1985). A device for cultivation of plant and animal cells. Biotechnol. Lett 7: 467–47. 10.1007/BF01199860 Web of Science®Google Scholar Singh V. (1999). Disposable bioreactor for cell culture using wave-induced motion. Cytotechnology 30: 149–158. 10.1023/A:1008025016272 CASPubMedWeb of Science®Google Scholar Lehmann J, Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H. (1992). Der Superspinner—Ein Brutschrankfermenter für die Massenkultur tierischer Zellen. BioEngineering 5/6: 112–117. Google Scholar De Jesus MJ, Girard P, Bourgeois M, Baumgartner G, Kacko B, Amstutz H, Wurm FM. (2004). TubeSpin satellites: A fast track approach for process development with animal cells using shaking technology. Biochem. Eng. J. 17: 217–223. 10.1016/S1369-703X(03)00180-3 CASWeb of Science®Google Scholar Muller N, Girard P, Hacker D, Jordan M, Wurm FM. (2004). Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol. Bioeng. 89: 400–406. 10.1002/bit.20358 CASWeb of Science®Google Scholar De Jesus MJ, Wurm FM. (2009). Medium and process optimization for high yield, high density suspension cultures: From low throughput spinner flasks to high throughput millilitre reactors. BioProcess Int. 7 (Suppl. 1): 12–17. Google Scholar Zhang X, Bürki CA, Stettler M, De Sanctis D, Perrone M, Discacciati M, Parolini N, DeJesus M, Hacker DL, Quarteroni A, Wurm FM. (2009). Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L. Biochem. Eng. J. 45: 41–47. 10.1016/j.bej.2009.02.003 CASWeb of Science®Google Scholar Zhang X, Stettler M, De Sanctis D, Perrone M, Parolini N, Discacciati M, De Jesus M, Hacker D, Quarteroni A, Wurm F. (2009). Use of orbital shaken disposable bioreactors for mammalian cell cultures from the mL scale to the 1,000 L scale. In D Eibl, R Eibl (eds.), Disposable Bioreactors, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 115. Berlin; Heidelberg: Springer, pp. 33–53. 10.1007/10_2008_18 Web of Science®Google Scholar Jia Q, Li H, Hui M, Hui N, Joudi A, Rishton G, Bao L, Shi M, Zhang X, Luanfeng L, Xu J, Leng G. (2008). A bioreactor system based on a novel oxygen transfer method. BioProcess Int. 6: 66–78. CASWeb of Science®Google Scholar Potera C. (2009). Firm on quest to improve biomanufacturing. Genet Eng. Biotechnol. News 7: 20–21. Google Scholar Werner S, Nägeli M. (2007). Good vibrations. BioTechnology 3: 22–24. Google Scholar Kauling J, Brod H, Schmidt S, Poggel M, Frahm B, Rose R. (2007). Einweg-Bioreaktor. Patent DE 102006018824A1. Google Scholar Davis RM, Taylor G. (1950). The mechanics of large bubbles rising through liquids in tubes. Proc. R Soc. Lond. A 200: 375–392. 10.1098/rspa.1950.0023 Web of Science®Google Scholar Nicklin DJ, Wilkes JO, Davidson JF. (1962). Two-phase flow in vertical tubes. Trans. Inst. Chem. Engrs. 40: 61–68. CASGoogle Scholar Terrier B, Courtois C, Hénault N, Cuvier A, Bastin M, Aknin A, Dubreuil J, Pétiard V. (2007). Two new disposable bioreactors for plant cell cultures: The wave & undertow bioreactor and the slug bubble bioreactor. Biotechnol. Bioeng. 96: 914–923. 10.1002/bit.21187 CASPubMedWeb of Science®Google Scholar Ducos JP, Terrier B, Courtois D, Pétiard V. (2008). Improvement of plastic-based disposable bioreactors for plant science needs. Phytochem. Rev. 7: 607–613. 10.1007/s11101-008-9089-1 CASGoogle Scholar Ducos JP, Terrier B, Courtois D. (2009). Disposable bioreactors for plant micropropagation and cell cultures. In D Eibl, R Eibl (eds.), Disposable Bioreactors, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 115. Berlin; Heidelberg: Springer, pp. 89–115. 10.1007/10_2008_28 Web of Science®Google Scholar Peacock L, Auton KA. (2008). Comparing shaker flasks with a single-use bioreactor for growing yeast seed cultures. BioProcess Int. 6: 54–57. CASGoogle Scholar Heath C, Kiss R. (2007). Cell culture process development: Advances in process engineering. Biotechnol. Prog. 23: 46–51. 10.1021/bp060344e CASPubMedWeb of Science®Google Scholar Tutorial. (2005). High-yield single-use cell culture systems. Genet Eng. Biotechnol. News. Available: http://www.genengnews.com/articles/chtitem.aspx?tid=1093&chid=3. Accessed August 22, 2009. Google Scholar Drugmand JC, Havelange N, Debras F, Collignon F, Mathieu E, Castillo J. (2009). Human and animal vaccine production in a new disposable fixed-bed bioreactor. Available: http://www.artelis.be./uploads/pdf.POSTER%20ARTEFIX%20BD.pdf. Accessed August 22, 2009. Google Scholar Glaser V. (2009). Bioreactor and fermentor trends. Genet Eng. Biotechnol. News. Available: http://www.genengnews.com/issues/item.aspx. Accessed August 22, 2009. Google Scholar Schreyer HB, Miller SE, Rodgers S. (2007). Application note: High-throughput process development. Genet Eng. Biotechnol. News. Available: http://www.genengnews.com/issues.com/issues/item.aspx?issue_id=78. Accessed August 22, 2009. Google Scholar Rao G, Moreira A, Brorson K. (2009). Disposable bio-processing: The future has arrived. Biotechnol. Bioeng. 102: 348–356. 10.1002/bit.22192 CASPubMedWeb of Science®Google Scholar Houtzager E, van der Linden R, de Roo G, Huurman S, Priem P, Sijmons PC. (2005). Linear scale-up of cell cultures. The next level in disposable bioreactor design. BioProcess Int. 6: 60–66. Google Scholar Eibl R, Eibl D. (2006). Design and use of the Wave bioreactor for plant cell culture. In S Dutta Gupta, Y Ibaraki (eds.), Plant Tissue Culture Engineering, Series: Focus on Biotechnology, Vol. 6. Dordrecht, The Netherlands: Springer, pp. 203–227. 10.1007/1-4020-3694-9_12 Google Scholar Knevelman C, Hearle DC, Osman JJ, Khan M, Dean M, Smith M, Aiyedebinu Cheung K. (2002). Characterization and operation of a disposable bioreactor as a replacement for conventional steam-in-place inoculum bioreactors for mammalian cell culture processes. Available: http://www.5.gelifesciences.com/aptrix/upp01077.nsf/Content/wave_bioreactor_home-wave_literature_WindowsInternetExplorer. Accessed August 23, 2009. Google Scholar Hitchcock T. (2009). Production of recombinant whole-cell vaccines with disposable manufacturing systems. BioProcess Int. 5: 36–45. Google Scholar D'Avino A, Zijlstra G, Oosterhuis N, van der Berg H. (2009). High cell density cultivation of PER.C6 cells in the CELL-tainer single-use bioreactor. Available: http://www.cellutionbiotech.com/Technology/Literature/image/ESACT-2009-PERC6-DSM. Accessed August 22, 2009. Google Scholar Lonza. (2008). CELL-tainer single-use bioreactors. Walkersville, Brochure. Google Scholar Paschedag AR. (2004). CFD in der Verfahrenstechnik. Weinheim: Wiley-VCH. 10.1002/3527603859 Google Scholar Menter FR. (1993). Zonal two equation k-ω turbulence models for aerodynamic flows. AIAA Paper: 93–2906. Google Scholar Öncül AA, Kalmbach A, Genzel Y, Reichl U, Thèvenin D. (2009). Numerische und experimentelle Untersuchung der Fliessbedingungen in Wave-Bioreaktoren. CIT 81: 1241. Google Scholar Haldankar R, Li D, Saremi Z, Baikalov C, Deshpande R. (2006). Serum-free suspension large-scale transient transfection of CHO cells in Wave bioreactors. Mol. Biotechnol. 34: 191–199, 10.1385/MB:34:2:191 CASPubMedWeb of Science®Google Scholar Genzel Y, Behrendt I, Koenig S, Sann H, Reichl U. (2004). Metabolism of MDCK cells during cell growth and influence on virus production in large-scale microcarrier culture. Vaccine 22: 2202–2208. 10.1016/j.vaccine.2003.11.041 CASPubMedWeb of Science®Google Scholar Genzel Y, Olmer RM, Schaefer B, Reichl U. (2006). Wave microcarrier cultivation of MDCK cells for influenza virus production in serum containing and serum-free media. Vaccine 24: 6074–6087. 10.1016/j.vaccine.2006.05.023 CASPubMedWeb of Science®Google Scholar Rios M. (2006). Process considerations for cell-based influenza vaccines. Pharm. Technol. 4: 1–6. Google Scholar Slivac I, Srček VG, Radoševic K, Kmetič I, Kniewald Z. (2006). Aujeszky's disease virus production in disposable bioreactors. J. Biosci. 3: 363–368. 10.1007/BF02704109 Web of Science®Google Scholar Hundt B, Best C, Schlawin N, Kassner H, Genzel Y, Reichl U. (2007). Establishment of a mink enteritis vaccine production process in stirred-tank reactor and Wave®Bioreactor microcarrier cultures in 1–10 L scale. Vaccine 25: 3987–3995. 10.1016/j.vaccine.2007.02.061 CASPubMedWeb of Science®Google Scholar Hami LS, Ghana H, Yuan V, Craig S. (2003). Comparison of a static process and a bioreactor-based process for the GMP manufacture of autologous Xcellerated T cells for clinical trials. BioProcessing J. 2: 1–10. Google Scholar Hami LS, Green C, Leshinsky N, Markham E, Miller K, Craig S. (2004). GMP production of Xcellerated T cells for the treatment of patients with CLL. Cytotherapy 6: 554–562. 10.1080/14653240410005348 CASPubMedWeb of Science®Google Scholar Levine B. (2007). Making waves in cell therapy: The Wave bioreactor for the generation of adherent and non-adherent cells for clinical use. Available: http://www.wavebiotech.com/pdf/literature/ISCT_2007_Levine_Final.pdf. Accessed November 4, 2007. Google Scholar Ritala A, Wahlström EH, Holkeri H, Hafren A, Mäkelainen K, Baez J, Mäkinen K, Nuutila AM. (2008). Production of a recombinant industrial protein using barley cell cultures. Protein Expr. Purif. 59: 274–281. 10.1016/j.pep.2008.02.013 CASPubMedWeb of Science®Google Scholar Jablonski-Lorin C, Mellio V, Hungerbühler E. (2003). Stereoselective bioreduction to a chiral building block on a kilogram scale. Chimia 57: 574–576. 10.2533/000942903777678894 CASWeb of Science®Google Scholar Ries C. (2008). The process engineering characteristics of the Thermo Fisher Scientific Single-Use Bioreactor 50 L: Determination of mixing time, power input and kLa values. Application note. Available from Thermo Fisher Scientific. Google Scholar Ozturk SS. (2007). Comparison of product quality: Disposable and stainless steel bioreactor. BioProduction 2007, Berlin, Germany. Google Scholar Tollnik C. (2009). Einsatz von Disposables in der Praxis—ein Erfahrungsbericht zu Design und Betrieb einer Pilotanlage für klinische Wirkstoffproduktionen. 2. Konferenz Einsatz von Single-Use-Disposables (Concept Heidelberg). Mannheim, Germany. Google Scholar Valentine P. (2009). Implementation of a single-use stirred bioreactor at pilot and GMP manufacturing scale for mammalian cell culture. ESACT 2009 Meeting, Dublin, Ireland. Google Scholar Mardirosian D, Guertin P, Crowell J, Yetz-Aldape J, Hall M, Hodge G, Jonnalagadda K, Holmgren A, Galliher P. (2009). Scaling up a CHO-produced hormone-protein fusion product. BioProcess Int. 7 (Suppl. 4): 30–35. 10.12665/J74.Varghese CASGoogle Scholar Galliher P. (2008). Achieving high-efficiency production with microbial technology in a single-use bioreactor platform. BioProcess Int. 11: 60–65. Google Scholar De Wilde D, Noack U, Kahlert W, Barbaroux M, Greller G. (2009). Bridging the gap from reusable to single-use manufacturing with stirred, single-use bioreactors. BioProcess Int. 7 (Suppl.4): 36–41. CASGoogle Scholar Castillo J, Vanhamel S. (2007). Cultivating anchorage-dependent cells. Genet Eng. Biotechnol. News 16: 40–41. Google Scholar Zambaux JP. (2007). How synergy answers the biotech industry needs. BioProduction 2007, Berlin, Germany. Google Scholar Zambeaux JP, Vanhamel S, Bosco F, Castillo J. (2007). Disposable bioreactor. Patent EP 1961606A2. Google Scholar Limke T. (2009). Comparability between the Mobius CellReady 3 L bioreactor and 3 L glass bioreactors. BioProcess Int. 7: 122–123. Google Scholar Maier U, Büchs J. (2001). Characterization of the gas-liquid mass transfer in shaking bioreactors. Biochem. Eng. J. 7: 99–106. 10.1016/S1369-703X(00)00107-8 CASPubMedWeb of Science®Google Scholar Gupta A, Rao G. (2003). A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnol. Bioeng. 84: 351–358. 10.1002/bit.10740 CASPubMedWeb of Science®Google Scholar Maier U, Losen M, Büchs J. (2004). Advances in understanding and modeling the gas-liquid mass transfer in shake flasks. Biochem. Eng. J. 17: 155–167. 10.1016/S1369-703X(03)00174-8 CASWeb of Science®Google Scholar Kato Y, Peter CP, Akgün A, Büchs J. (2004). Power consumption and heat transfer resistance in large rotary shaking vessels. Biochem. Eng. J. 21: 83–91. 10.1016/j.bej.2004.04.011 CASWeb of Science®Google Scholar Zhang H, Williams-Dalson W, Keshavarz-Moore E, Shamlou PA. (2005). Computational-fluid-dynamics (CFD) analysis of mixing and gas-liquid mass transfer in shake flasks. Biotechnol. Appl. Biochem. 41: 1–8. 10.1042/BA20040082 CASPubMedWeb of Science®Google Scholar Wurm FM. (2007). Novel technologies for rapid and low cost provisioning of antibodies and process details in mammalian cell culture-based biomanufacturing. BioProduction 2007, Berlin, Germany. Google Scholar Ries C, John C, Eibl R. (2009). Einwegbioreaktoren für die Prozessentwicklung mit Insektenzellen. Bioforum 3: 11–13. Google Scholar Liu CM, Hong LN. (2001). Development of a shaking bio-reactor system for animal cell cultures. Biochem. Eng. J. 2: 121–125. 10.1016/S1369-703X(00)00111-X Web of Science®Google Scholar Raval K, Liu CM, Büchs J. (2006). Large-scale disposable shaking bioreactors. BioProcess Int. 1: 46–49. Google Scholar Eibl R, Kaiser S, Lombriser R, Eibl D. (2010). Disposable bioreactors: The current state-of-the-art and recommended applications in biotechnology. Appl. Microbiol. Biotechnol. DOI: 10.1007/s00253-009-2422-9. Google Scholar Liu CZ, Towler MJ, Medrano G, Cramer CL, Weathers PJ. (2009). Production of mouse interleukin-12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor. Biotechnol. Bioeng. 102: 1074–1086. 10.1002/bit.22154 CASPubMedWeb of Science®Google Scholar Weathers PJ, Towler MJ, Xu J. (2010). Bench to batch: Advances in plant cell culture for producing useful products. Appl. Microbiol. Biotechnol. 85: 1339–1351. 10.1007/s00253-009-2354-4 CASPubMedWeb of Science®Google Scholar D Eibl, R Eibl (eds.). (2009). Disposable Bioreactors, Series: Advances in Biochemical Engineering/Biotechnology, Vol. 115. Berlin; Heidelberg: Springer. Google Scholar Citing Literature Single‐Use Technology in Biopharmaceutical Manufacture ReferencesRelatedInformation
Publication Year: 2010
Publication Date: 2010-12-08
Language: en
Type: other
Indexed In: ['crossref']
Access and Citation
Cited By Count: 30
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot