Title: Construction and expression of hCHD5 gene in eukaryotic.
Abstract: Objective:To construct an expression vector that can express CHD5 gene in eukaryotic. Methods:The human gene CHD5 was amplified from human brain cDNA by PCR. The expression vector peGFPc1-CHD5 was constructed by DNA recombination. The constructed plasmid was transformed into 293T cell by lipofectamine 2000. The expression level of CHD5 mRNA in293T cell was determined by real-time PCR. Results:The human gene CHD5 was effectively amplified. The expression vector peGFPc1-CHD5 was constructed,the DNA sequencing result showed that the constructed plasmid containing CHD5 gene was the same as designed. Real-time PCR analysis showed that the recombinant plasmid peGFPc1-CHD5 could express the CHD5 mRNA in 293T cell. Conclusions:The human gene CHD5 has been successfully cloned and expressed,which could be used as the tool for research on function and mechanism of CHD5 gene.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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