Title: DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF GRANISETRON HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORMS
Abstract: Article history A simple, rapid, accurate, sensitive and precise stability indicating UV Spectrophotometric method has been developed for the quantitative estimation of Granisetron hydrochloride (GTH) in bulk and tablet dosage forms. Granisetron hydrochloride exhibited maximum wavelength for absorption at 301 nm in 0.1 N HCL as solvent with molar absorptivity of 1.34 × 10 4 L mole -1 cm -1 . Beer's law was found to be obeyed in the concentration range of 5-30μg/ml with correlation coefficient value of 0.9995. The limit of detection and limit of quantification were found to be 0.54μg/ml & 2.02μg/ml respectively. Results of the analysis were validated as per ICH guidelines. Forced degradation studies include the effect of temperature, peroxide oxidation, photolysis and susceptibility to hydrolysis across a wide range of pH values, were carried out according to the ICH requirements. The developed method was validated respect to linearity, precision and accuracy. The proposed stability indicating UV Spectrophotometric method is useful for the routine estimation of Granisetron hydrochloride in bulk and tablet dosage forms.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot