Title: Biochemical basis of resistance in wheat on inoculation with cereal cyst nematode, Heterodera avenae.
Abstract: Biochemical basis of nematode disease resistance was studied by analysis of the activity of three enzymes, peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) in the shoot and root tissues of resistant and susceptible genotypes of wheat before and after inoculation with the cereal cyst nematode H. avenae. The biochemical studies were conducted with the parent cultivars/lines, the F1 population, F2, BC1 and test cross populations of the cross Raj 1482 × CCNRV1 (Raj MR1) An elevated level of enzyme activity was observed in all the inoculated plants both in the susceptible and resistant cultivars, though, the resistant cultivars have shown maximum enzyme activity in comparison to the susceptible cultivars. The root tissues had shown increased enzyme activity in comparison to the shoot tissues, irrespective of nematode inoculation. Among the three biochemical enzymes studied, PAL had shown the maximum enzyme activity, in both the shoots and roots irrespective of susceptible and resistant cultivars. Analysis of enzyme activity in the cross between Raj1482 × Raj MR1, depicted that F1 generation had shown tremendous increase in the enzyme activity in all the three enzymes studied, over either of the parents. The F2 population showed lower enzyme activity in comparison to F1 population. The results with BC1 (test cross) and BC1 population were almost at par. In comparison to phenylalanine ammonia lyase (PAL) and peroxidase (PO), Polyphenol-oxidase (PPO) did not reveal a significant difference in the control and inoculated plants irrespective of susceptible and resistant cultivar taken into consideration or the type of tissue (shoot/root) analysed.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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