Title: [7] Isolation and culture of marine nitrogen-fixing unicellular cyanobacteria Synechococcus
Abstract: Publisher Summary This chapter describes methods of sample collection, enrichment, and isolation. Culture methods for one of the fast-growing aerobic N 2 -fixing marine Synechococcus species are also described. Nitrogen-fixing cyanobacteria can be encountered in a variety of marine environments. Samples should be placed into sterilized plastic bags. Samples taken from the water column can be obtained with a discrete depth sampler, a number of which are available. Sediment samples can be obtained using a grab sampler or similar apparatus. Finally, as some cyanobacteria are known to grow epiphytically, samples of macroalgae and seagrasses can be taken along with scrapings from rocks and other stationary objects. Subsequent to the enrichment process the subsamples are examined microscopically, and those subsamples containing unicellular forms are singled out for further isolation procedures. This is initiated with a serial dilution process using the same enriched medium as above. The serial dilution is followed by plating on sterile agar media (enrichment media above in 2% agar), Streaking is also employed to help isolate monoclonal colonies.
Publication Year: 1988
Publication Date: 1988-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 24
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