Title: [8] The inhibition of catalase (hydrogen-peroxide: Hydrogen peroxide oxireductase, EC 1.11.1.6) by ascorbate
Abstract: This chapter describes the procedure for the preparation and assay of catalase. The inhibition of catalase by ascorbate is described in the chapter. A mechanism involving the inactivation of catalase (due to disintegration of the protein) by free radicals generated during the auto-oxidation of ascorbate is discussed in the chapter. The presence of divalent cations (particularly Cu2+ and Fe2+) and Fe3+ during the auto-oxidation greatly increases this inhibition. Catalytic activity is determined by measuring the decrease in absorbance at 240mμ when catalase is incubated with H2O2. The measurements can be made manually or, more simply, by a recording spectrophotometer. Assay procedure involves the detection of inhibition of catalase by ascorbate and factors affecting the inhibition.
Publication Year: 1970
Publication Date: 1970-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 19
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot