Title: Chapter 23 Methods for Assessing the Binding of Steroid Hormones in Nuclei
Abstract: Publisher Summary This chapter describes the methods for assessing the binding of steroid hormones in nuclei. When a steroid hormone enters a target cell, it binds to a receptor molecule that is then translocated into the nucleus. There it associates with nuclear sites and stimulates a sequence of events resulting in a series of biological responses. The binding of receptor-steroid complexes by nuclei can be studied by several methods. A radioactive steroid can be administered to intact animals or to cell or organ cultures, the nuclei isolated, and the content of radioactivity then measured directly. Nuclei can be isolated from cells and then incubated with radioactive receptor–steroid complexes prepared independently. Nuclei can be isolated and the receptor–steroid complexes measured by incubation with an excess of radioactive steroid at an elevated temperature permitting exchange with the unlabeled steroid. The difficulty of this assay is the technical problem of high nonspecific steroid binding in nuclei of tissues. To circumvent this problem, an alternative nuclear exchange assay is discussed in the chapter. Some of the methods developed and used to examine the binding of steroid receptors to isolated nuclei are also discussed.
Publication Year: 1978
Publication Date: 1978-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 1
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