Title: Enzyme Activity Analysis of Protease Produced by Marine Bacteria
Abstract: Proteolytic enzymes are ubiquitous in nature, found in all living organisms, and have been applied to many aspects of industry. In this study, 19 strains hydrolyzing protein were screened from deep-sea sediments, of which No. 9 strain has the largest protease activity. Based on morphological characterization and 16S rDNA gene sequences analysis, the strain was identified to be Bacillus subtilis. The optimum medium for producing protease was determined through orthogonal test as follows: 2.5 % xylose, 3.5 % peptone, 1.6 % yeast extract and 1 % NaCl, the highest protease activity reached 215.95 U/mL, and increased by 128.06 % than the origin.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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