Title: Abstract #LB-249: Modulation of gene hypermethylation in colorectal cancer cells by anthocyanins from black raspberries
Abstract: AACR Annual Meeting-- Apr 18-22, 2009; Denver, CO
Hypermethylation of tumor suppressor gene promoters is a distinguishing characteristic of cancer genomes and plays an important role in the pathogenesis of many tumor types including colorectal cancer. Our laboratory has shown that anthocyanins are among the most effective cancer preventive compounds in black raspberries (BRBs). In the present study, we determined whether BRB anthocyanins render positive effects on aberrant methylation of genes in human colorectal cancer cells. The colorectal cancer cell line, HCT116, was treated every 24 hours for three days with a 5 \#956;g/ml anthocyanin-rich fraction of BRBs. The methylation status of 24 cancer-related genes in anthocyanin-treated and untreated cells was investigated using restriction enzyme digestion combined with quantitative PCR and methylation specific PCR. Notable genes that exhibited decreased methylation with anthocyanin treatment include PTEN , p16 , TNFRSF10c , and HIC1 . This study has recently been extended to examine the effect of anthocyanin-containing BRB powder on the methylation status of genes in colorectal tumor specimens taken from patients who were treated for 2-4 weeks with 60g/day of BRB powder administered orally in water. The results of these investigations will be presented. This is the first study of the effects of anthocyanins on hypermethylation of cancer-associated genes. Our results suggest a possible mechanism for the preventive abilities of anthocyanins in diets with attainable doses and reasonable treatment periods. The ability of anthocyanins to induce promoter demethylation in colon cancer cells is encouraging considering the high availability of these compounds in the human diet. Supported by NCI grant CA103180 and USDA grant 38903-03560 .
Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr LB-249.
Publication Year: 2009
Publication Date: 2009-05-01
Language: en
Type: article
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