Publication Information

{'id': 'https://openalex.org/W805590754', 'doi': None, 'title': 'Design and Experimental Testing of Nanoinjection Protocols for Delivering Molecules into HeLa Cells with a Bio-MEMS Device', 'display_name': 'Design and Experimental Testing of Nanoinjection Protocols for Delivering Molecules into HeLa Cells with a Bio-MEMS Device', 'publication_year': 2014, 'publication_date': '2014-01-01', 'ids': {'openalex': 'https://openalex.org/W805590754', 'mag': '805590754'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5034&context=etd', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5051905054', 'display_name': 'Zachary K. Lindstrom', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I100005738', 'display_name': 'Brigham Young University', 'ror': 'https://ror.org/047rhhm47', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I100005738']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Zachary Kendall Lindstrom', 'raw_affiliation_strings': ['Brigham Young Univ'], 'affiliations': [{'raw_affiliation_string': 'Brigham Young Univ', 'institution_ids': ['https://openalex.org/I100005738']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': ['https://openalex.org/A5051905054'], 'corresponding_institution_ids': ['https://openalex.org/I100005738'], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 66}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9915, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9915, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9907, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10613', 'display_name': 'Virus-based gene therapy research', 'score': 0.9903, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/propidium-iodide', 'display_name': 'Propidium iodide', 'score': 0.86406946}, {'id': 'https://openalex.org/keywords/hela', 'display_name': 'HeLa', 'score': 0.7776574}, {'id': 'https://openalex.org/keywords/viability-assay', 'display_name': 'Viability assay', 'score': 0.6390616}], 'concepts': [{'id': 'https://openalex.org/C2775934118', 'wikidata': 'https://www.wikidata.org/wiki/Q424169', 'display_name': 'Propidium iodide', 'level': 4, 'score': 0.86406946}, {'id': 'https://openalex.org/C2777366897', 'wikidata': 'https://www.wikidata.org/wiki/Q847482', 'display_name': 'HeLa', 'level': 3, 'score': 0.7776574}, {'id': 'https://openalex.org/C53227056', 'wikidata': 'https://www.wikidata.org/wiki/Q189152', 'display_name': 'Viability assay', 'level': 3, 'score': 0.6390616}, {'id': 'https://openalex.org/C24107716', 'wikidata': 'https://www.wikidata.org/wiki/Q123619', 'display_name': 'Nucleic acid', 'level': 2, 'score': 0.5704477}, {'id': 'https://openalex.org/C186852380', 'wikidata': 'https://www.wikidata.org/wiki/Q1142521', 'display_name': 'Electroporation', 'level': 3, 'score': 0.48293823}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.46828315}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.42317355}, {'id': 'https://openalex.org/C171250308', 'wikidata': 'https://www.wikidata.org/wiki/Q11468', 'display_name': 'Nanotechnology', 'level': 1, 'score': 0.42048666}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.39881408}, {'id': 'https://openalex.org/C1491633281', 'wikidata': 'https://www.wikidata.org/wiki/Q7868', 'display_name': 'Cell', 'level': 2, 'score': 0.39764258}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.38448572}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.35660988}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.29755265}, {'id': 'https://openalex.org/C192562407', 'wikidata': 'https://www.wikidata.org/wiki/Q228736', 'display_name': 'Materials science', 'level': 0, 'score': 0.2691481}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.25533694}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.23371285}, {'id': 'https://openalex.org/C190283241', 'wikidata': 'https://www.wikidata.org/wiki/Q14599311', 'display_name': 'Apoptosis', 'level': 2, 'score': 0.19566566}, {'id': 'https://openalex.org/C31573885', 'wikidata': 'https://www.wikidata.org/wiki/Q304484', 'display_name': 'Programmed cell death', 'level': 3, 'score': 0.14483887}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5034&context=etd', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.47, 'id': 'https://metadata.un.org/sdg/16', 'display_name': 'Peace, justice, and strong institutions'}, {'score': 0.41, 'id': 'https://metadata.un.org/sdg/10', 'display_name': 'Reduced inequalities'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 48, 'referenced_works': ['https://openalex.org/W1496782356', 'https://openalex.org/W1520821663', 'https://openalex.org/W1554324421', 'https://openalex.org/W1880293219', 'https://openalex.org/W1968458490', 'https://openalex.org/W1968800607', 'https://openalex.org/W1973579525', 'https://openalex.org/W1982532269', 'https://openalex.org/W1982757342', 'https://openalex.org/W1988338936', 'https://openalex.org/W1989227183', 'https://openalex.org/W1990509864', 'https://openalex.org/W1997108574', 'https://openalex.org/W1998386880', 'https://openalex.org/W2007353945', 'https://openalex.org/W2009070325', 'https://openalex.org/W2018391369', 'https://openalex.org/W2025528973', 'https://openalex.org/W2028597062', 'https://openalex.org/W2032579724', 'https://openalex.org/W2034416132', 'https://openalex.org/W2037105403', 'https://openalex.org/W2049013068', 'https://openalex.org/W2049621351', 'https://openalex.org/W2049980461', 'https://openalex.org/W2050030633', 'https://openalex.org/W2056367325', 'https://openalex.org/W2059262359', 'https://openalex.org/W2067038367', 'https://openalex.org/W2070909031', 'https://openalex.org/W2078483179', 'https://openalex.org/W2079756782', 'https://openalex.org/W2082781273', 'https://openalex.org/W2085879913', 'https://openalex.org/W2099507346', 'https://openalex.org/W2131087094', 'https://openalex.org/W2131767946', 'https://openalex.org/W2133163037', 'https://openalex.org/W2140031668', 'https://openalex.org/W2142319129', 'https://openalex.org/W2148911589', 'https://openalex.org/W2169790726', 'https://openalex.org/W2466241608', 'https://openalex.org/W2490696883', 'https://openalex.org/W2565076352', 'https://openalex.org/W2740319795', 'https://openalex.org/W806077861', 'https://openalex.org/W971851131'], 'related_works': ['https://openalex.org/W3142122616', 'https://openalex.org/W3024510762', 'https://openalex.org/W3007536231', 'https://openalex.org/W2949803925', 'https://openalex.org/W2908858721', 'https://openalex.org/W2904257715', 'https://openalex.org/W2858192943', 'https://openalex.org/W2778295886', 'https://openalex.org/W2773136784', 'https://openalex.org/W2581087628', 'https://openalex.org/W2559969061', 'https://openalex.org/W2551423860', 'https://openalex.org/W2337970408', 'https://openalex.org/W2323808264', 'https://openalex.org/W2299191356', 'https://openalex.org/W2086400534', 'https://openalex.org/W2072376005', 'https://openalex.org/W2057196210', 'https://openalex.org/W2024152888', 'https://openalex.org/W1993056838'], 'abstract_inverted_index': {'Design': [0], 'and': [1, 37, 70, 76, 134, 162, 282, 289, 342, 439], 'Experimental': [2], 'Testing': [3], 'of': [4, 21, 26, 40, 105, 114, 116, 144, 189, 227, 303, 318, 409, 427, 437], 'Nanoinjection': [5], 'Protocols': [6], 'for': [7, 60, 212], 'Delivering': [8, 28], 'Molecules': [9], 'into': [10, 31, 48, 83, 170, 185, 218, 242, 273, 420], 'HeLa': [11, 117, 192, 219, 274], 'Cells': [12], 'with': [13, 267, 339, 349, 369], 'a': [14, 35, 86, 153, 190, 213, 248, 268, 350, 370], 'Bio-MEMS': [15], 'Device': [16], 'Zachary': [17], 'K.': [18], 'Lindstrom': [19], 'Department': [20], 'Mechanical': [22], 'Engineering,': [23], 'BYU': [24], 'Master': [25], 'Science': [27], 'foreign': [29], 'molecules': [30, 82, 313], 'living': [32], 'cells': [33, 49, 84, 119, 171, 237, 261, 288, 296, 307], 'is': [34, 55, 323], 'broad': [36], 'ongoing': [38], 'area': [39, 59], 'research.': [41], 'Gene': [42], 'therapy,': [43], 'or': [44, 52, 80, 368], 'delivering': [45, 81], 'nucleic': [46, 160], 'acids': [47, 161], 'via': [50], 'non-viral': [51], 'viral': [53], 'pathways,': [54], 'an': [56, 103, 145, 223, 375], 'especially': [57], 'promising': [58, 416], 'pharmaceutics.': [61], 'All': [62], 'gene': [63, 232, 441], 'therapy': [64], 'methods': [65], 'have': [66, 239, 431], 'their': [67], 'respective': [68], 'advantages': [69], 'disadvantages,': [71], 'including': [72], 'limited': [73], 'delivery': [74, 334, 360, 418], 'efficiency': [75, 133], 'low': [77, 345], 'viability.': [78, 136, 198], 'Nanoinjection,': [79], 'using': [85, 172], 'solid': [87, 106], 'lance,': [88], 'has': [89, 414], 'proven': [90], 'to': [91, 129, 140, 159, 187, 229, 255, 280, 287, 306, 325, 364, 381, 394], 'be': [92], 'highly': [93], 'efficient': [94], 'while': [95, 195], 'maintaining': [96, 196], 'high': [97], 'viability': [98], 'levels.': [99], 'In': [100, 336, 405], 'this': [101], 'thesis,': [102], 'array': [104, 181], 'silicon': [107], 'lances': [108], 'was': [109, 138, 168, 216, 253, 278, 308, 379, 392], 'tested': [110], 'by': [111, 403], 'nanoinjecting': [112], 'tens': [113], 'thousands': [115], 'cancer': [118], 'simultaneously.': [120], 'Several': [121], 'molecule': [122, 149, 359, 417], 'types': [123], 'were': [124, 271], 'injected': [125], 'in': [126, 203, 222, 236, 245, 260, 293, 356, 384, 397, 434], 'different': [127, 249], 'tests': [128, 365], 'understand': [130, 328], 'cell': [131, 135, 193], 'uptake': [132, 401], 'Voltage': [137], 'used': [139], 'determine': [141, 256], 'the': [142, 173, 179, 210, 243, 246, 319, 329, 357, 410, 428, 435], 'impact': [143], 'electric': [146], 'field': [147, 436], 'on': [148], 'delivery.': [150], 'Propidium': [151], 'iodide,': [152], 'dye': [154], 'that': [155, 178, 291, 331, 424], 'fluoresces': [156], 'when': [157, 166, 362], 'bound': [158], 'does': [163], 'not': [164], 'fluoresce': [165], 'unbound,': [167], 'delivered': [169, 286, 305], 'lance': [174, 180], 'array.': [175], 'Results': [176], 'show': [177], 'delivers': [182], 'propidium': [183, 204, 340, 399], 'iodide': [184, 205, 341, 400], 'up': [186, 228], '78%': [188], 'nanoinjected': [191, 217, 272], 'culture,': [194], '78%-91%': [197], 'Using': [199], 'similar': [200], 'protocol': [201], 'as': [202], 'experiments,': [206], 'plasmid': [207], 'DNA': [208, 241, 250, 258, 264, 285, 290, 304, 312, 333, 343], 'containing': [209], 'code': [211], 'fluorescent': [214], 'protein': [215], 'cells,': [220, 422], 'resulting': [221], 'average': [224, 301, 398], 'expression': [225, 233], 'rate': [226], '0.21%.': [230], 'Since': [231], 'only': [234], 'occurs': [235], 'which': [238], 'integrated': [240], 'genome': [244], 'nucleus,': [247], 'detection': [251], 'method': [252], 'developed': [254, 380], 'total': [257], 'count': [259], 'following': [262, 297], 'nanoinjection.': [263, 298], 'strands': [265], 'tagged': [266], 'radioactive': [269, 320], 'isotope': [270], 'cells.': [275], 'Liquid': [276], 'scintillation': [277], 'employed': [279], 'quantify': [281], 'discriminate': [283], 'between': [284], 'remained': [292], 'solution': [294], 'around': [295], 'The': [299, 389], 'largest': [300], 'amount': [302], '20.0': [309], 'x': [310], '103': [311], 'per': [314], 'cell.': [315], 'Further': [316], 'development': [317], 'nanoinjection': [321, 377, 387, 412], 'process': [322, 413, 429], 'needed': [324], 'more': [326], 'fully': [327], 'parameters': [330], 'affect': [332], 'efficiency.': [335], 'all': [337], 'experiments': [338], 'molecules,': [344], 'accumulation': [346], 'voltage,': [347, 354], 'coupled': [348], 'short': [351], 'pulsed': [352], 'release': [353], 'resulted': [355], 'greatest': [358], 'efficiencies': [361], 'compared': [363], 'without': [366], 'voltage': [367, 372], 'constant': [371], 'only.': [373], 'Lastly,': [374], 'automated': [376, 390], 'system': [378, 391], 'eliminate': [382], 'variability': [383, 396], 'user': [385], 'applied': [386], 'force.': [388], 'found': [393], 'reduce': [395], 'values': [402], '56%.': [404], 'conclusion,': [406], 'experimental': [407], 'testing': [408], 'multi-cell': [411], 'shown': [415], 'results': [419], 'human': [421], 'suggesting': [423], 'further': [425], 'optimization': [426], 'would': [430], 'positive': [432], 'implications': [433], 'academic': [438], 'clinical': [440], 'therapy.': [442]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W805590754', 'counts_by_year': [], 'updated_date': '2024-12-06T04:55:36.909179', 'created_date': '2016-06-24'}