Abstract: Objective To study the effects of interleukin-10 on expressions of IL-10 and IL-10 receptor (IL-10R) mRNA in hepatic stellate cells in experimental liver fibrosis rats. Methods Sixty SD rats were randomly divided into control group (group A), liver fibrotic group (group B) and IL-10 treated group (group C). Fibrotic group and IL-10 treated group were injected intraperitoneally with carbon tetrachloride (CCl4). IL-10 treated group was given IL-10 intraperitoneally from the third week. Hepatic stellate cells were isolated from these rats at the seventh and eleventh week during the course of liver fibrosis, respectively. The total RNAs were extracted. The expressions of IL-10 and IL-10R mRNA in HSCs were measured by RT-PCR. Results The CCl4-induced experimental rat liver fibrosis model was established successfully. The hepatic stellate cells were extracted successfully. The expressions of IL-10 and IL-10R mRNA in group B significantly increased compared to those in control group. But the gradual decrease was observed in the expression of IL-10 during hepatic fibrogenesis. These changes were partially reversed by simultaneous administration of IL-10.After being treated with IL-10, the expressions of IL-10 and IL-10R mRNA were higher than those in group B, especially in the 11th week. Conclusion The HSCs of rats could express IL-10 and IL-10R. The IL-10 can play the antifibrogenic effect on HSCs.
Publication Year: 2010
Publication Date: 2010-01-20
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot