Title: Detection of genetically modified maize by multiplex PCR-gene chip.
Abstract: Based on integrated DNAs structure of seven genetically modified maize (Zea mays), Bt11, Bt176, Mon810, Mon863, TC1507, GA21 and NK603, event specific primers were designed, and the PCR system and program were optimized. The results indi- cated that the sensitivity of PCR detection was 0.1%. In addition, two multiplex PCR reactions were separately developed by specific fragments with different lengths in Bt11, Mon810, TC1507 and GA21, NK603 and Bt176 which could be amplified simultaneously in one PCR reaction. Meanwhile, maize endogenous reference gene ZSSIIb could also be detected. Based on the unique and specific in- tegration junction sequences between the host plant genome DNA and the integrated gene, a 40mer Oligo probes with high specificity have being developed. Then the transgenic detection DNA chips were got. The results indicated that the sensitivity of microarray detection was 0.01% and the event specific oligonucleotide probes could meet the detecting requirements of sensitivity and specificity as well as high efficiency. One set of 3×multiplex PCR system and two sets of 4×multiplex PCR system for detecting and identifying transgenic maize were set up and optimized. Different genes could be detected specifically in one oligonucleotide microarray using multiplex PCR-gene chip which improved the detection efficiency and accuracy.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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