Title: MUTAGENIC ACTION OF SOME RADIOMIMETIC AGENTS ON T2 COLIPHAGE
Abstract: The mutagenic action of various cytostatic agents used in cancer therapy were assayed by comparing their effect on the mutation rate of T2h/sup +/ phage with the spontaneous rate. Myleran increased the rate 10 fold, and a lesser degree of mutagenicity was found with the following drugs (in decreasing order of effectiveness): A139 (2,5-bis-methoxyethoxy3,6-bis -ethyleneiminobenzoquinone), Mitarson, Endoxan, N-oxide mustard, N-methyl mustard, and N mustard. Their lethal doses in the phage and effect on Escherichia coli B generation time were also determined. The mutagenic action of the N mustards was correlated with the release of chloride ion. Mutagenic action of the agents tested was most pronounced at concentrations approximates 10/sup -5/M; at higher concentrations cytotoxic effects predominated over mutagenic ones. Demonstration of mutagenic activity of N mustards was carried out in phosphate buffer; it was confirmed that this activity is readily lost in bicarbonate buffer. (H.H.D.)
Publication Year: 1963
Publication Date: 1963-01-01
Language: en
Type: article
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